黄芪甲苷对乌头碱诱导的斑马鱼心脏损伤保护作用的研究

详细信息    查看全文 | 推荐本文 |

英文篇名：Study on the Protective Effect of Astragaloside Ⅳ on Aconitine-Induced Zebrafish Heart Injury 作者：石亚楠 ; 王佑华 ; 李强 ; 姚磊 ; 符德玉 英文作者：SHI Ya-nan;WANG You-hua;LI Qiang;YAO Lei;FU De-yu;Yueyang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Translational Medical Center for Development and Disease Institute of Pediatric, Shanghai Key Laboratory of Birth Defect Children's Hospital of Fudan University; 关键词：黄芪甲苷 ; 斑马鱼 ; 乌头碱 ; 心脏损伤 ; 心率 英文关键词：Astragaloside Ⅳ;;zebrafish;;aconitine;;heart damage;;heart rate 中文刊名：HNZB 英文刊名：Guiding Journal of Traditional Chinese Medicine and Pharmacy 机构：上海中医药大学附属岳阳医院;上海中医药大学附属龙华医院;复旦大学附属儿科医院儿科研究所上海市出生缺陷防治重点实验室; 出版日期：2019-04-15 出版单位：中医药导报 年：2019 期：v.25;No.329 语种：中文; 页：HNZB201907005 页数：3 CN：07 ISSN：43-1446/R 分类号：29-31

摘要

目的:探讨不同浓度的黄芪甲苷对乌头碱诱导的斑马鱼心脏损伤的保护作用。方法:挑选受精后发育正常的48 h斑马鱼胚胎,用30 mg/L的乌头碱溶液药浴,同时给予10、25、40μg/m L的黄芪甲苷溶液,观察斑马鱼的心率、心脏形态以及静脉窦-动脉球(SV-BA)距离的变化,观察黄芪甲苷对其心脏损伤的保护作用。结果:30 mg/L的乌头碱溶液处理24 h可致斑马鱼心率加快、心包水肿、SV-BA间距延长。25、40μg/m L黄芪甲苷溶液能降低斑马鱼的心率,减轻斑马鱼心包水肿的程度。结论:黄芪甲苷对乌头碱诱导的斑马鱼心脏损伤具有一定的保护作用,且呈剂量依赖性。
        Objective: To investigate the protective effect of astragaloside Ⅳ at different concentrations on aconitine-induced zebrafish heart injury. Methods: Zebrafish of 48 hours post fertilization were treated with 30 mg/L aconitine solution and 10, 25, and 40 μg/m L astragaloside Ⅳ solution for 24 h. We observed the heart rate,heart shape, and SV-BA of zebrafish. Results: The 30 mg/L aconitine solution can cause zebrafish heart injury such as heart rate increase, ventricular arrest, pericardial edema. The study found that 25 and 40 μg/m L astragaloside Ⅳ solution can reduce the heart rate of zebrafish and relieve the extent of pericardium edema. Conclusion: The Astragaloside Ⅳ shows a certain protective effect on aconitine-induced cardiac damage in zebrafish,which is dose-dependent.

引文

[1] Xu X L,Ji H,Gu S Y,et al. Modification of alterations in cardiac function and sarcoplasmic reticulum by astragaloside IV in myocardial injury in vivo[J].Eur J Pharmacol,2007,568(1/3):203.
    [2] Zhang W D,Chen H,Zhang C,et al. Astragaloside IV from Astragalus membranaceus shows cardioprotection during myocardialischemia in vivo and in vitro[J].Planta Med,2006,72(1):4-8.
    [3] Zhao Z,Wang W,Wang F,et al. Effects of astragaloside IV onheart failure in rats[J].Chin Med,2009(4):1-6.
    [4]方芳,赵杰,余林中,等.乌头碱(Aconitine)对斑马鱼心脏毒性的初步研究[J].中药药理与临床,2012,28(2):31-33.
    [5]武琳,杨明,陈忠.斑马鱼模型在心血管疾病研究领域的应用及进展[J].现代药学,2018,46(3):354-357.
    [6] Daniel R. Brown, Leigh Ann Samsa, Li Q, et al. Advances in the Study of Heart Development and Disease Using Zebrafish[J].Cardiovasc Dev Dis,2016(3):13.
    [7]吴晓敏,何秋霞,韩利文,等.阿司咪唑对斑马鱼心脏毒性的初步研究[J].中国药理学通报,2013,29(9):1251-1254.
    [8]陈可冀,付长庚.黄芪在心血管疾病中的临床应用[J].中国循证心血管医学杂志,2014,6(5):509-511.
    [9]薛迪,韩利文,杨继芳,等.白藜芦醇对阿司咪唑诱导斑马鱼心脏损伤的保护作用[J].山东科学,2015,28(3):29-33.
    [10]朱晓宇,刘洪翠,俞航萍,等.神香苏合丸防治心血管疾病的实验研究[J].浙江理工大学学报(自然科学版),2016,35(4):630-635.
    [11]李智平,韩利文,何秋霞,等.原花青素对特非那定诱导的斑马鱼心脏损伤的保护作用[J].中药药理与临床,2017,33(3):54-57.
    [12]田真,马丕勇,杨春燕,等.乌头碱(Aconitine)诱发大鼠心律失常的研究[J].中国实验诊断学,2016,20(9):1447-1448.
    [13]张宪明,王天英.炙甘草汤治疗缓慢型心律失常65例的疗效观察[J].中国医药导报,2007,4(29):72.
    [14]程齐来,李洪亮,张道英.藏红花酸抗心律失常作用研究[J].解放军医学杂志,2010,35(4):395-397.
    [15]陈育群,张鹏飞,黄政德.桂枝甘草龙骨牡蛎汤治疗心律失常疗效及安全性的系统评价[J].中医药导报,2018,24(10):113-116,120.
    [16]晏程远,陈云,刘云云,等.柴胡加龙牡复律汤抗大鼠室性心律失常的实验研究[J].中医药导报,2014,20(7):20-22.