洋桔梗组培苗炼苗研究
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摘要
[目的]建立高效而稳定的洋桔梗组培苗炼苗技术。[方法]以洋桔梗带芽茎段离体培养的组培苗为供试材料,在不同基质、温度和幼苗根长条件下开展组培苗的成活率、根系和茎叶生长研究。[结果]洋桔梗组培苗炼苗的最适基质为草炭土∶蛭石∶珍珠岩=2∶2∶1(V∶V),最适温度为25℃,最适的根长为4.0 cm。[结论]该研究为建立高效而稳定的洋桔梗组培苗的炼苗和移栽技术提供了基础资料。
[Objective] To establish a highly efficient and stable technology of hardening seedling for tissue culture seedling of Eustoma grandiflorum.[Method] Taking tissue culture seedlings which were obtained by stem with young bud of seedlings of E.grandiflorum as experimental materials,the survival rate of the tissue culture seedling,growth development of the roots and the stem leaves were studied under the condition of different cultivation substrate,temperature,root length of seedling.[Result]The hardening seedling substrate consisted turf+roseite+perlite with 2∶2∶1(V∶V) was the best.The best temperature was 25 ℃,and the best root length of tissue culture seedling was 4.0 cm while tissue culture seedling hardening of E.grandiflorum.[Conclusion]The study provides a basic data for establishing a highly efficient and stable technology of hardening seedling for tissue culture seedling of E.grandiflorum.
[Objective] To establish a highly efficient and stable technology of hardening seedling for tissue culture seedling of Eustoma grandiflorum.[Method] Taking tissue culture seedlings which were obtained by stem with young bud of seedlings of E.grandiflorum as experimental materials,the survival rate of the tissue culture seedling,growth development of the roots and the stem leaves were studied under the condition of different cultivation substrate,temperature,root length of seedling.[Result]The hardening seedling substrate consisted turf+roseite+perlite with 2∶2∶1(V∶V) was the best.The best temperature was 25 ℃,and the best root length of tissue culture seedling was 4.0 cm while tissue culture seedling hardening of E.grandiflorum.[Conclusion]The study provides a basic data for establishing a highly efficient and stable technology of hardening seedling for tissue culture seedling of E.grandiflorum.
引文
[1]陈小凤,龚明霞,康德贤,等.国内洋桔梗组培快繁技术的研究进展[J].北方园艺,2008(6):67-69.
[2]李竹英,吴军.洋桔梗品种发芽试验[J].北方园艺,2011(19):63-65.
[3]陆继亮.洋桔梗有望成为云南又一强势鲜切花品种[J].中国花卉园艺,2012(5):36.
[4]潘绍斌,邢定生.众花商通海争睹洋桔梗新品种[N].玉溪日报,2010-07-02(006).
[5]王瑷玲.洋桔梗的栽培(一)[J].园林,1999(3):22-23.
[6]黄正秉.洋桔梗遭遇育种育苗难题[N].中国花卉报,2012-05-01(010).
[7]孙敏杰,张晓艳,吴媛媛.不同基质配比对洋桔梗育苗的影响[J].园艺与种苗,2012(8):1-3.
[8]苏琛.洋桔梗组培技术研究[J].安徽农业科学,2011,39(30):18417-18418.
[9]贺美忠,王桂梅,杨富.桔梗组培快繁技术研究[J].山西农业科学,2009,37(12):9-11.
[10]孙慧晶,李建宾,希从芳,等.两种植物生长调节剂对洋桔梗离体培养的效应[J].云南农业大学学报,2014,29(2):208-215.
[11]张子学,丁为群,乔华伟,等.洋桔梗的快速繁殖研究[J].中国林副特产,2005,77(4):1-3.
[12]钟波.洋桔梗组培苗生产关键技术研究[J].北方园艺,2012(16):90-92.
[13]赵红霞,高文强,张利,等.杜鹃组培苗的练苗技术研究[J].北方园艺,2012(7):75-76.
[14]谢文申,周露,许锐.薄荷试管苗练苗移栽技术[J].北方园艺,2012(18):133-134.
[15]周文兰.蝴蝶兰组培苗的炼苗移栽技术研究[J].现代园艺,2012(6):6-8.
[16]蒲海萍.丽格海棠组培苗炼苗移栽技术[J].现代农业科技,2009(14):188.
[17]李群,刘光勇,王丽.激素对洋桔梗植株再生的影响及生根培养的研究[J].广西植物,2004,24(1):40-42.
[18]马洪英.天津地区洋桔梗引种观察试验[J].北方园艺,2012(12):67-71.
[19]姜跃丽,叶桦.洋桔梗簇叶化现象探析[J].现代农业科技,2009(17):198-199.
[20]李金泽,刘关所,杨春梅,等.洋桔梗6大种植难点分析[J].中国园艺文摘,2015(4):156-158.
[21]万珠珠,谭秀梅,牛来春,等.不同低温春化天数和赤霉素处理对解除洋桔梗莲座化的影响[J].北方园艺,2016(14):62-65.
[2]李竹英,吴军.洋桔梗品种发芽试验[J].北方园艺,2011(19):63-65.
[3]陆继亮.洋桔梗有望成为云南又一强势鲜切花品种[J].中国花卉园艺,2012(5):36.
[4]潘绍斌,邢定生.众花商通海争睹洋桔梗新品种[N].玉溪日报,2010-07-02(006).
[5]王瑷玲.洋桔梗的栽培(一)[J].园林,1999(3):22-23.
[6]黄正秉.洋桔梗遭遇育种育苗难题[N].中国花卉报,2012-05-01(010).
[7]孙敏杰,张晓艳,吴媛媛.不同基质配比对洋桔梗育苗的影响[J].园艺与种苗,2012(8):1-3.
[8]苏琛.洋桔梗组培技术研究[J].安徽农业科学,2011,39(30):18417-18418.
[9]贺美忠,王桂梅,杨富.桔梗组培快繁技术研究[J].山西农业科学,2009,37(12):9-11.
[10]孙慧晶,李建宾,希从芳,等.两种植物生长调节剂对洋桔梗离体培养的效应[J].云南农业大学学报,2014,29(2):208-215.
[11]张子学,丁为群,乔华伟,等.洋桔梗的快速繁殖研究[J].中国林副特产,2005,77(4):1-3.
[12]钟波.洋桔梗组培苗生产关键技术研究[J].北方园艺,2012(16):90-92.
[13]赵红霞,高文强,张利,等.杜鹃组培苗的练苗技术研究[J].北方园艺,2012(7):75-76.
[14]谢文申,周露,许锐.薄荷试管苗练苗移栽技术[J].北方园艺,2012(18):133-134.
[15]周文兰.蝴蝶兰组培苗的炼苗移栽技术研究[J].现代园艺,2012(6):6-8.
[16]蒲海萍.丽格海棠组培苗炼苗移栽技术[J].现代农业科技,2009(14):188.
[17]李群,刘光勇,王丽.激素对洋桔梗植株再生的影响及生根培养的研究[J].广西植物,2004,24(1):40-42.
[18]马洪英.天津地区洋桔梗引种观察试验[J].北方园艺,2012(12):67-71.
[19]姜跃丽,叶桦.洋桔梗簇叶化现象探析[J].现代农业科技,2009(17):198-199.
[20]李金泽,刘关所,杨春梅,等.洋桔梗6大种植难点分析[J].中国园艺文摘,2015(4):156-158.
[21]万珠珠,谭秀梅,牛来春,等.不同低温春化天数和赤霉素处理对解除洋桔梗莲座化的影响[J].北方园艺,2016(14):62-65.