初发急性白血病患者骨髓乳腺癌耐药蛋白基因表达及其与预后关系的研究
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摘要
目的研究初发急性白血病患者骨髓乳腺癌耐药蛋白(BCRP)基因表达及其与预后关系的相关性。方法选取2014年1月-2014年12月入住该院血液内科的急性白血病患者67例,同时选择50例健康志愿者作为对照组。通过实时荧光定量PCR检测骨髓BCRP基因表达,并分析比较骨髓BCRP基因表达和临床预后的关系。结果急性白血病患者骨髓BCRP基因表达高于对照组(P<0.01),按照BCRP均值(3.0)将患者分为BCRP高表达组和BCRP低表达组。BCRP高表达组患者初始WBC计数较BCRP低表达组高(P<0.01),且两组生存分析比较差异有统计学意义(Log-rank=21.78,P<0.05)。结论急性白血病患者骨髓BCRP基因表达高于健康志愿者,且BCRP高表达患者的预后更差。
Objective To investigate the gene expression of breast cancer resistance protein(BCRP) in bone marrow of acute leukemia(AL) and its prognosis. Methods A total of 67 AL patients admitted in our hospital from January 2014 to December 2014 were enrolled in the study, and 10 healthy volunteers were involved as control group. Real-time PCR was utilized to measure the gene expression of BCRP in bone marrow. And the difference in the BCRP gene expression and its prognosis were analyzed. Results The gene expression of BCRP in bone marrow of AL patients was statistically higher than that in the healthy volunteer group(P < 0.01). Acute leukemia patients were divided into high BCRP group and low BCRP group according to the mean of BCRP(3.0). Compared with low BCRP group, the primary WBC count was higher in BCRP high group(P < 0.01). The survival analysis between the two groups was statistically significant(Log-rank = 21.78, P < 0.05). Conclusion The gene expression of BCRP in bone marrow of AL patients was statistically increased and associated with the prognosis. High BCRP gene expression was a risk factor for poor prognosis in patients with AL.
Objective To investigate the gene expression of breast cancer resistance protein(BCRP) in bone marrow of acute leukemia(AL) and its prognosis. Methods A total of 67 AL patients admitted in our hospital from January 2014 to December 2014 were enrolled in the study, and 10 healthy volunteers were involved as control group. Real-time PCR was utilized to measure the gene expression of BCRP in bone marrow. And the difference in the BCRP gene expression and its prognosis were analyzed. Results The gene expression of BCRP in bone marrow of AL patients was statistically higher than that in the healthy volunteer group(P < 0.01). Acute leukemia patients were divided into high BCRP group and low BCRP group according to the mean of BCRP(3.0). Compared with low BCRP group, the primary WBC count was higher in BCRP high group(P < 0.01). The survival analysis between the two groups was statistically significant(Log-rank = 21.78, P < 0.05). Conclusion The gene expression of BCRP in bone marrow of AL patients was statistically increased and associated with the prognosis. High BCRP gene expression was a risk factor for poor prognosis in patients with AL.
引文
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[11]吴祥猛,盛莉,贾雨霏,等.乳腺癌耐药蛋白在药物代谢动力学中的作用[J].药学学报,2016,51(9):1368-1377.
[12]李薇,周明,罗科玲,等.急性髓系白血病中乳腺癌耐药蛋白基因表达与临床意义[J].中国现代医学杂志,2016,26(2):29-32.
[13]VY G,FILION M.New developments in quantitative real-time polymerase chain reaction technology[J].Curr Issues Mol Biol,2014,16(1):1-5.
[2]FORAN J M.New prognostic markers in acute myeloid leukemia:perspective from the clinic[J].Hematology,2010,2010(1):47-55.
[3]NOGUCHI K,KATAYAMA K,MITSUHASHI J,et al.Functions of the breast cancer resistance protein(BCRP/ABCG2)in chemotherapy[J].Adv Drug Deliv Rev,2009,61(1):26-33.
[4]BRACKMAN DEANNA J,GIACOMINI KATHLEEN M.Reverse translational research of ABCG2(BCRP)in human disease and drug response[J].Clin.Pharmacol.Ther,2018,103(2):233-242.
[5]中华医学会血液学分会.成人急性髓系白血病(非急性早幼粒细胞白血病)中国诊疗指南(2011年版)[J].中华血液学杂志,2011,32(11):804-807.
[6]ADNAN M,MORTON G,HADI S.Analysis of rpo S and bol Agene expression under various stress-induced environments in planktonic and biofilm phase using 2(-ΔΔCT)method[J].Mol Cell Biochem,2011,357(1):275-282.
[7]LIN T L,SMITH B D.Prognostically important molecular markers in cytogenetically normal acute myeloid leukemia[J].Am J Med Sci,2011,341(5):404-408.
[8]de MORAES A C,MARANHO C K,RAUBER G S,et al.Importance of detecting multidrug resistance proteins in acute leukemia prognosis and therapy[J].J Clin Lab Anal,2013,27(1):62-71.
[9]顾卓伟,狄文.应用ABC转运蛋白抑制剂治疗肿瘤化疗耐药的现状与对策[J].现代妇产科进展,2015(4):299-302.
[10]NATARAJAN K,XIE Y,BAER M R,et al.Role of breast cancer resistance protein(BCRP/ABCG2)in cancer drug resistance[J].Biochem Pharmacol,2012,83(8):1084-1103.
[11]吴祥猛,盛莉,贾雨霏,等.乳腺癌耐药蛋白在药物代谢动力学中的作用[J].药学学报,2016,51(9):1368-1377.
[12]李薇,周明,罗科玲,等.急性髓系白血病中乳腺癌耐药蛋白基因表达与临床意义[J].中国现代医学杂志,2016,26(2):29-32.
[13]VY G,FILION M.New developments in quantitative real-time polymerase chain reaction technology[J].Curr Issues Mol Biol,2014,16(1):1-5.
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