摘要
比较两个金线莲Anoectochilus roxburghii (Wall.) Lindl.种质的生理生化特性及内源激素含量差异,为后续的金线莲优良品种选育提供参考依据。本研究以福建小圆叶金线莲和福建黑叶金线莲30 d扩繁丛生苗、30 d生根试管苗和30 d温室移栽苗为材料,采用酶联免疫吸附测定法(ELISA)分别测定两个金线莲种质的内源激素含量;可溶性蛋白、可溶性糖、叶绿素和丙二醛(MDA)含量,以及过氧化物酶(POD)活性采用分光光度法测定。试验结果显示在扩繁、生根、移栽过程中,福建小圆叶金线莲可溶性蛋白含量、可溶性总糖含量、POD活性、MDA含量及其体内脱落酸(ABA)、赤霉素(GA)含量均高于福建黑叶金线莲,且两个不同种质金线莲扩繁、生根苗的可溶性蛋白、可溶性总糖及其体内玉米素(ZR)、GA和生长素(IAA)含量均显著(P<0.05)高于移栽苗。因此,福建小圆叶金线莲可能更易于适应环境的变化。
The physiological and biochemical characteristics and endogenous hormone content of two germplasms of Anoectochilus roxburghii were compared to provide reference for the subsequent breeding of fine varieties of A.roxburghii. The 30-day of propagation cluster seedlings,30-day of rooting tube seedlings and 30-day of greenhouse transplanting seedlings of Fujian A.roxburghii with small round leaves and Fujian A.roxburghii with black leaves were used as materials. The content of endogenous hormones in two germplasms of A. roxburghii was determined by enzyme-linked immunosorbent assay(ELISA). The contents of soluble protein,soluble sugar,chlorophyll,malondialdehyde(MDA) and the activity of Peroxidase(POD) in two germplasms of A.roxburghii were measured by spectrophotometry. The results showed that during the process of propagation,rooting and transplanting,the contents of soluble protein,total soluble sugar,MDA,ABA,GA and the activity of POD in A.roxburghii with small round leaves were higher than those in A. roxburghii with black leaves. Moreover,in the process of propagation and rooting,the contents of soluble protein,total soluble sugar,ZR,GA and IAA in two germplasms of A.roxburghii were significantly(P<0.05) higher than those of transplanting seedlings. Therefore,A.roxburghii with small round leaves may be easier to adapt to changes in the environment.
引文
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