编织碳纤维表面胶原/HA涂层的研究
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摘要
采用两种不同的方法在编织碳纤维表面制备了胶原与羟基磷灰石(HA)复合涂层.研究了不同制备方法对涂层的影响,并对复合涂层的物相,元素组成,官能团以及生物活性进行了表征与测试.研究结果表明,利用电沉积法复合浸渍提拉法可以在编织碳纤维表面制备均匀致密的胶原/HA复合涂层.模拟体液浸泡实验表明,编织碳纤维表面所制备的胶原/HA复合涂层,具有优异的生物活性.本研究为以编织碳纤维为支架的人工气管生物材料的研究奠定了理论基础.
Collagen and hydroxyapatite(HA)composite coatings on the surface of woven carbon fibers were prepared by two different methods.Effects of different preparation methods on composite coatings were investigated.The phase,element composition,functional group and biological activity of the composite coating were characterized.It is shown that the uniform and dense collagen/HA composite coating can be prepared on the surface of woven carbon fibers by the combination of electrochemical deposition and dipping method.Simulated body fluid immersion experiments shown that the collagen/HA composite coating prepared on the woven carbon fibers surface has an excellent bioactivity.This work laid the theoretical foundation for the study of artificial tracheal biomaterials with woven carbon fiber as scaffold.
Collagen and hydroxyapatite(HA)composite coatings on the surface of woven carbon fibers were prepared by two different methods.Effects of different preparation methods on composite coatings were investigated.The phase,element composition,functional group and biological activity of the composite coating were characterized.It is shown that the uniform and dense collagen/HA composite coating can be prepared on the surface of woven carbon fibers by the combination of electrochemical deposition and dipping method.Simulated body fluid immersion experiments shown that the collagen/HA composite coating prepared on the woven carbon fibers surface has an excellent bioactivity.This work laid the theoretical foundation for the study of artificial tracheal biomaterials with woven carbon fiber as scaffold.
引文
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[12]Perez R A,Altankov G,Jorgeherrero E,et al.Micro-and nanostructured hydroxyapatite-collagen microcarriers for bone tissue-engineering applications[J].Journal of Tissue Engineering&Regenerative Medicine,2013,7(5):323-343.
[13]Li H,Zhao X,Cao S,et al.Na-doped hydroxyapatite coating on carbon/carbon composites:Preparation,in vitro bioactivity and biocompatibility[J].Applied Surface Science,2012,263(24):163-173.
[14]赵雪妮,王婉英,王旭东,等.镁掺杂羟基磷灰石的制备及其生物活性研究[J].陕西科技大学学报(自然科学版),2017,35(2):61-65.
[15]徐艳丽,林龙翔,耿志旺,等.电化学共沉积HA/胶原复合涂层及其生物性能初探[J].电化学,2007,13(3):238-241.
[16]Raftery R M,Woods B,Marques A L,et al.Multifunctional biomaterials from the sea:Assessing the effects of chitosan incorporation into collagen scaffolds on mechanical and biological functionality[J].Acta Biomaterialia,2016,43:160-169.
[17]Kane R J,Weiss Bilka H E,Meagher M J,et al.Hydroxyapatite reinforced collagen scaffolds with improved architecture and mechanical properties[J].Acta Biomaterialia,2015,17(6):16-25.
[18]Sukul M,Min Y K,Lee B T.Collagen-hydroxyapatite coated unprocessed cuttlefish bone as a bone substitute[J].Materials Letters,2016,181:156-160.
[2]李咏玲.人工气管材料的研究[J].医学信息,2015,28(2):374.
[3]史宏灿,徐志飞,秦雄,等.人工气管组分材料胶原蛋白/羟基磷灰石体外细胞毒理学研究[J].生物医学工程学杂志,2003,20(4):608-611.
[4]Cholas R,Kunjalukkal P S,Gervaso F,et al.Scaffolds for bone regeneration made of hydroxyapatite microspheres in a collagen matrix[J].Materials Science&Engineering C Materials for Biological Applications,2016,63:499-505.
[5]Wang X,Zhao X,Wang W,et al.Controllable preparation of a nano-hydroxyapatite coating on carbon fibers by electrochemical deposition and chemical treatment[J].Materials Science&Engineering C,2016,63:96-105.
[6]Kwak S,Haider A,Gupta K C,et al.Micro/Nano multilayered scaffolds of PLGA and collagen by alternately electrospinning for bone tissue engineering[J].Nanoscale Research Letters,2016,11(1):1-16.
[7]Zhang Y,Tan S,Yin Y.C-fibre reinforced hydroxyapatite bioceramics[J].Ceramics International,2003,29(1):113-116.
[8]Wang X,Zhao X,Zhang L,et al.Design and fabrication of carbon fibers with needle-like nano-HA coating to reinforce granular nano-HA composites[J].Materials Science and Engineering C,2017,77:765-771.
[9]何剑鹏,赵雪妮,张靖,等.碳纤维表面电沉积制备钙磷涂层的研究[J].陕西科技大学学报(自然科学版),2015,33(3):68-72.
[10]Wang X G.Mechanical properties and microstructure of short carbon fiber reinforced hydroxyapatite bio-composite[J].Materials Science Forum,2011,685:357-361.
[11]Murphy C M,Schindeler A,Gleeson J P,et al.A collagenhydroxyapatite scaffold allows for binding and co-delivery of recombinant bone morphogenetic proteins and bisphosphonates[J].Acta Biomaterialia,2014,10(5):2 250-2 258.
[12]Perez R A,Altankov G,Jorgeherrero E,et al.Micro-and nanostructured hydroxyapatite-collagen microcarriers for bone tissue-engineering applications[J].Journal of Tissue Engineering&Regenerative Medicine,2013,7(5):323-343.
[13]Li H,Zhao X,Cao S,et al.Na-doped hydroxyapatite coating on carbon/carbon composites:Preparation,in vitro bioactivity and biocompatibility[J].Applied Surface Science,2012,263(24):163-173.
[14]赵雪妮,王婉英,王旭东,等.镁掺杂羟基磷灰石的制备及其生物活性研究[J].陕西科技大学学报(自然科学版),2017,35(2):61-65.
[15]徐艳丽,林龙翔,耿志旺,等.电化学共沉积HA/胶原复合涂层及其生物性能初探[J].电化学,2007,13(3):238-241.
[16]Raftery R M,Woods B,Marques A L,et al.Multifunctional biomaterials from the sea:Assessing the effects of chitosan incorporation into collagen scaffolds on mechanical and biological functionality[J].Acta Biomaterialia,2016,43:160-169.
[17]Kane R J,Weiss Bilka H E,Meagher M J,et al.Hydroxyapatite reinforced collagen scaffolds with improved architecture and mechanical properties[J].Acta Biomaterialia,2015,17(6):16-25.
[18]Sukul M,Min Y K,Lee B T.Collagen-hydroxyapatite coated unprocessed cuttlefish bone as a bone substitute[J].Materials Letters,2016,181:156-160.