短期温度、盐度胁迫对海洋青鳉鱼(Oryziasmelastigma)摄食行为及抗氧化的影响
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摘要
本研究通过设置温度突变组(15°C、25°C、31°C)和盐度突变组(5、15、25、35),探讨了温度、盐度变化胁迫对海洋青鳉鱼(Oryzias melastigma)摄食行为及抗氧化生理的影响。结果表明,在温度为15°C和31°C(盐度为25)时以及盐度为5和35(温度为25°C)条件下,青鳉鱼摄食响应时间增长,摄食量、摄食效率和摄食成功率显著下降(P<0.05)。超氧化物歧化酶(SOD)、过氧化氢酶(CAT)酶活性和丙二醛(MDA)含量在15—31°C时随温度增加先降低后增加。SOD酶活性31°C时第7天显著性低于对照组(P<0.05)。MDA含量在15°C和31°C时在显著性高于对照组,第7天显著性升高(P<0.05)。不同盐度处理组,SOD、CAT活性和MDA含量均随盐度增加先降低后增加,MDA含量在盐度为5和35时均显著高于对照组(P<0.05)。综上,海洋青鳉鱼在盐度15—25,水温25°C条件下摄食及游动行为活跃,抗氧化活性最低,生长良好。当温度高于31°C或低于15°C;盐度高于35或低于5时胁迫对青鳉鱼摄食行为及抗氧化性造成明显影响。
The effects of variable temperature acute change(15°C, 25°C, 31°C) and salinity acute change(5, 15, 25, 35)on the feeding behavior and antioxidant of marine medaka(Oryzias melastigma) were investigated. The results show that the feeding response time increased, while the feed intake, feeding efficiency, and feeding success rate decreased significantly(P<0.05) at a temperature of 15°C and 31°C. The SOD, CAT activities, and MDA content increased first and then decreased with the temperature increased from 15°C to 30°C. The SOD activities increased significantly at 31°C group on the 7 th day(P<0.05). The MDA content significantly higher than the control group and increased significantly(P<0.05) at the 7 th day at the temperature of 15°C and 31°C. Also, SOD, CAT activities, and MDA content decreased at first and then increased with increased salinity from 5 to 35, and the content of MDA was significantly higher(P<0.05)than that of the control. Therefore, we believe that a temperature or salinity acute change can affect significantly the feeding behavior and antioxidant system of O. melastigma, and the suitable temperature was 25°C, salinity was 15—25.
The effects of variable temperature acute change(15°C, 25°C, 31°C) and salinity acute change(5, 15, 25, 35)on the feeding behavior and antioxidant of marine medaka(Oryzias melastigma) were investigated. The results show that the feeding response time increased, while the feed intake, feeding efficiency, and feeding success rate decreased significantly(P<0.05) at a temperature of 15°C and 31°C. The SOD, CAT activities, and MDA content increased first and then decreased with the temperature increased from 15°C to 30°C. The SOD activities increased significantly at 31°C group on the 7 th day(P<0.05). The MDA content significantly higher than the control group and increased significantly(P<0.05) at the 7 th day at the temperature of 15°C and 31°C. Also, SOD, CAT activities, and MDA content decreased at first and then increased with increased salinity from 5 to 35, and the content of MDA was significantly higher(P<0.05)than that of the control. Therefore, we believe that a temperature or salinity acute change can affect significantly the feeding behavior and antioxidant system of O. melastigma, and the suitable temperature was 25°C, salinity was 15—25.
引文
王奇,范灿鹏,陈锟慈等,2010.三种磺胺类药物对罗非鱼肝脏组织中谷胱甘肽转移酶(GST)和丙二醛(MDA)的影响.生态环境学报,19(5):1014-1019
王涛,苗亮,李明云等,2013.突降盐度胁迫对大黄鱼(Pseudosciaena crocea)血清生理生化及鳃丝Na+/K+-ATP酶活性的影响.海洋与湖沼,44(2):421-426
王晓杰,连丞,降瑞婵等,2018.海洋酸化对青鳉鱼(Oryzias melastigma)耳石形态和成分的影响.海洋环境科学,37(2):168-174
王晓杰,肖潇,李超等,2015.海洋酸化对海水青鳉胚胎骨骼发育的影响.海洋学报,37(12):116-122
王海亮,温海深,张晓燕,2016.盐度胁迫对花鲈幼鱼肠道抗氧化和非特异性免疫能力的影响.现代农业科技,(4):261-263,269
尹飞,孙鹏,彭士明等,2011.低盐度胁迫对银鲳幼鱼肝脏抗氧化酶、鳃和肾脏ATP酶活力的影响.应用生态学报,22(4):1059-1066
叶富良,张健东,2002.鱼类生态学.广州:广东高等教育出版社,2-84
伍辛泷,黄乾生,方超等,2012.新兴海洋生态毒理学模式生物--海洋青鳉鱼(Oryzias melastigma).生态毒理学报,7(4):345-353
刘峰,刘阳阳,楼宝等,2016.温度对小黄鱼体内抗氧化酶及消化酶活性的影响.海洋学报,38(12):76-85
孙儒泳,张玉书,1982.温度对罗非鱼生长的影响.生态学报,2(2):181-188
余德光,杨宇晴,王海英等,2011.盐度变化对斜带石斑鱼生理生化因子的影响.水产学报,35(5):719-728
张春禄,陈超,李炎璐等,2017.盐度对条纹锯鮨(Centropristis striata)仔鱼的活力、开口、摄食的影响.渔业科学进展,38(2):77-82
张晨捷,张艳亮,高权新等,2015.低盐胁迫对黄姑鱼幼鱼肝脏抗氧化功能的影响.南方水产科学,11(4):59-64
陈瑶,方志俊,郑金树等,2010.海洋青鳉鱼专用型基因芯片的设计及其在生态毒理学上的应用研究.应用海洋学学报,29(3):359-366
陈漪,王晓杰,冉皓宇等,2016.海水模式种青鳉鱼(Oryzias melastigma)的胚胎发育观察.海洋与湖沼,47(1):71-82
周银环,曹伏君,罗杰等,2017.不同温度梯度和规格体重对大弹涂鱼(Boleophthalmus pectinirostris)耗氧率(RO)和排氨率(RN)的影响.海洋与湖沼,48(2):392-397
柳旭东,张利民,王际英等,2008.盐度对水产动物体组成与组织结构的影响.养殖与饲料,(9):60-64
徐革锋,牟振波,韩英等,2015.温度和流速对细鳞鲑(Brachymystax lenok)幼鱼游泳能力的影响及其与呼吸代谢的关系.海洋与湖沼,46(4):786-792
郭黎,马爱军,王新安等,2012.盐度和温度对大菱鲆幼鱼抗氧化酶活性的影响.大连海洋大学学报,27(5):422-428
郭勤单,王有基,吕为群,2014.温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响.水生生物学报,38(1):58-67
黄玉瑶,1988.青鳉的生物学特性与饲养管理技术.动物学杂志,23(6):28-31
崔前进,陈冰,邱丽华等,2017.低盐胁迫对钝吻黄盖鲽幼鱼鳃Na+/K+-ATP酶、肝脏抗氧化酶和非特异免疫酶的影响.广东海洋大学学报,(6):26-32
梁国栋,王辉,刘加慧等,2014.罗非鱼仔鱼开口率与温度、盐度间模型的建立与优化.广东海洋大学学报,34(4):33-34
廖雅丽,张晨捷,彭士明等,2016.盐度对云纹石斑鱼抗氧化酶及溶菌酶活性的影响.上海海洋大学学报,25(2):169-176
Abou Anni I S,Bianchini A,Barcarolli I F et al,2016.Salinity influence on growth,osmoregulation and energy turnover in juvenile pompano Trachinotus marginatus Cuvier 1832.Aquaculture,455:63-72
Bisazza A,Butterworth B,Piffer L et al,2014.Collective enhancement of numerical acuity by meritocratic leadership in fish.Scientific Reports,4:4560
Canzian J,Fontana B D,Quadros V A et al,2017.Conspecific alarm substance differently alters group behavior of zebrafish populations:Putative involvement of cholinergic and purinergic signaling in anxiety-and fear-like responses.Behavioural Brain Research,320:255-263
Chen X P,Li L,Wong C K et al,2009.Rapid adaptation of molecular resources from zebrafish and medaka to develop an estuarine/marine model.Comparative Biochemistry and Physiology Part C:Toxicology&Pharmacology,149(4):647-655
De Azevedo R V,Dos Santos-Costa K,De Oliveira K F et al,2015.Responses of Nile tilapia to different levels of water salinity.Latin American Journal of Aquatic Research,43(5):828-835
Foster S J,Vincent A C J,2004.Life history and ecology of seahorses:implications for conservation and management.Journal of Fish Biology,65(1):1-61
Gabillard J C,Weil C,Rescan P Y et al,2003.Effects of environmental temperature on IGF1,IGF2,and IGF type Ireceptor expression in rainbow trout(Oncorhynchus mykiss).General and Comparative Endocrinology,133(2):233-242
Handeland S O,Berge?,Bj?rnsson B T et al,2000.Seawater adaptation by out-of-season Atlantic salmon(Salmo salar L.)smolts at different temperatures.Aquaculture,181(3-4):377-396
Handeland S O,Imsland A K,Stefansson S O,2008.The effect of temperature and fish size on growth,feed intake,food conversion efficiency and stomach evacuation rate of Atlantic salmon post-smolts.Aquaculture,283(1-4):36-42
Hergenrader G L,Hasler A D,1967.Seasonal changes in swimming rates of Yellow Perch in lake Mendota as measured by sonar.Transactions of the American Fisheries Society,96(4):373-382
Huang Z H,Ma A J,Wang X A et al,2015.Interaction of temperature and salinity on the expression of immunity factors in different tissues of juvenile turbot Scophthalmus maximus based on response surface methodology.Chinese Journal of Oceanology and Limnology,33(1):28-36
Jain K E,Farrell A P,2003.Influence of seasonal temperature on the repeat swimming performance of rainbow trout Oncorhynchus mykiss.Journal of Experimental Biology,206:3569-3579
Jobling M,1993.Bioenergetics:Feed intake and energy partitioning.In:Rankin J C,Jensen F B eds.Fish Ecophysiology.Dordrecht:Springer
Keen J E,Farrell A P,1994.Maximum prolonged swimming speed and maximum cardiac performance of rainbow trout,Oncorhynchus mykiss,acclimated to two different water temperatures.Comparative Biochemistry and Physiology Part A:Physiology,108(2-3):287-295
Kinoshita M,Murata K,Naruse K et al,2009.Medaka:Biology,Management,and Experimental Protocols.Iowa City,IA,Iowa,USA:Wiley-Blackwell,9-11,23-24
Lee H B,Kim D S,Gil H W et al,2018.Pysiological responses of diploid and triploid far eastern catfish,Silurus asotus to water temperature stress.Development&Reproduction,22(2):165-174
Livingstone D R,2001.Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms.Marine Pollution Bulletin,42(8):656-666
Luo Y P,Xie X J,2008.Effects of temperature on the specific dynamic action of the southern catfish,Silurus meridionalis.Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology,149(2):150-156
Meyer H A,Chipps S R,Graeb B D S et al,2016.Growth,food consumption,and energy status of juvenile pallid sturgeon fed natural or artificial diets.Journal of Fish and Wildlife Management,7(2):389-396
Mork O I,Bjerkeng B,Rye M,1999.Aggressive interactions in pure and mixed groups of juvenile farmed and hatchery-reared wild Atlantic salmon Salmo salar L.in relation to tank substrate.Aquaculture Research,30(8):571-578
Mourente G,Díaz-Salvago E,Bell J G et al,2002.Increased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream(Sparus aurata L.)fed dietary oxidised oil:attenuation by dietary vitamin E.Aquaculture,214(1-4):343-361
Rambo C L,Mocelin R,Marcon M et al,2017.Gender differences in aggression and cortisol levels in zebra fish subjected to unpredictable chronic stress.Physiology and Behavior,171:50-54
Rome L C,Funke R P,Alexander R M,1990.The influence of temperature on muscle velocity and sustained performance in swimming carp.Journal of Experimental Biology,154:163-178
Russell N R,Fish J D,Wootton R J,1996.Feeding and growth of juvenile sea bass:the effect of ration and temperature on growth rate and efficiency.Journal of Fish Biology,49(2):206-220
Sergio C V,Jesús T P P,Gerónimo R C et al,2013.Effects of temperature and salinity on growth and survival of the Pacific red snapper Lutjanus peru(Pisces:Lutjanidae)juvenile.Latin American Journal of Aquatic Research,41(5):1013-1018
Silverstein J T,Wolters W R,Shimizu M et al,2000.Bovine growth hormone treatment of channel catfish:strain and temperature effects on growth,plasma IGF-I levels,feed intake and efficiency and body composition.Aquaculture,190(1-2):77-88
Xiu M,Pan L Q,Jin Q et al,2015.Gender differences in detoxification metabolism of polycyclic aromatic hydrocarbon(chrysene)in scallop Chlamys farreri during the reproduction period.Comparative Biochemistry and Physiology Part C:Toxicology&Pharmacology,170:50-59
Zhou C,Zhang B H,Lin K et al,2017.Near-infrared imaging to quantify the feeding behavior of fish in aquaculture.Computers and Electronics in Agriculture,135:233-241
王涛,苗亮,李明云等,2013.突降盐度胁迫对大黄鱼(Pseudosciaena crocea)血清生理生化及鳃丝Na+/K+-ATP酶活性的影响.海洋与湖沼,44(2):421-426
王晓杰,连丞,降瑞婵等,2018.海洋酸化对青鳉鱼(Oryzias melastigma)耳石形态和成分的影响.海洋环境科学,37(2):168-174
王晓杰,肖潇,李超等,2015.海洋酸化对海水青鳉胚胎骨骼发育的影响.海洋学报,37(12):116-122
王海亮,温海深,张晓燕,2016.盐度胁迫对花鲈幼鱼肠道抗氧化和非特异性免疫能力的影响.现代农业科技,(4):261-263,269
尹飞,孙鹏,彭士明等,2011.低盐度胁迫对银鲳幼鱼肝脏抗氧化酶、鳃和肾脏ATP酶活力的影响.应用生态学报,22(4):1059-1066
叶富良,张健东,2002.鱼类生态学.广州:广东高等教育出版社,2-84
伍辛泷,黄乾生,方超等,2012.新兴海洋生态毒理学模式生物--海洋青鳉鱼(Oryzias melastigma).生态毒理学报,7(4):345-353
刘峰,刘阳阳,楼宝等,2016.温度对小黄鱼体内抗氧化酶及消化酶活性的影响.海洋学报,38(12):76-85
孙儒泳,张玉书,1982.温度对罗非鱼生长的影响.生态学报,2(2):181-188
余德光,杨宇晴,王海英等,2011.盐度变化对斜带石斑鱼生理生化因子的影响.水产学报,35(5):719-728
张春禄,陈超,李炎璐等,2017.盐度对条纹锯鮨(Centropristis striata)仔鱼的活力、开口、摄食的影响.渔业科学进展,38(2):77-82
张晨捷,张艳亮,高权新等,2015.低盐胁迫对黄姑鱼幼鱼肝脏抗氧化功能的影响.南方水产科学,11(4):59-64
陈瑶,方志俊,郑金树等,2010.海洋青鳉鱼专用型基因芯片的设计及其在生态毒理学上的应用研究.应用海洋学学报,29(3):359-366
陈漪,王晓杰,冉皓宇等,2016.海水模式种青鳉鱼(Oryzias melastigma)的胚胎发育观察.海洋与湖沼,47(1):71-82
周银环,曹伏君,罗杰等,2017.不同温度梯度和规格体重对大弹涂鱼(Boleophthalmus pectinirostris)耗氧率(RO)和排氨率(RN)的影响.海洋与湖沼,48(2):392-397
柳旭东,张利民,王际英等,2008.盐度对水产动物体组成与组织结构的影响.养殖与饲料,(9):60-64
徐革锋,牟振波,韩英等,2015.温度和流速对细鳞鲑(Brachymystax lenok)幼鱼游泳能力的影响及其与呼吸代谢的关系.海洋与湖沼,46(4):786-792
郭黎,马爱军,王新安等,2012.盐度和温度对大菱鲆幼鱼抗氧化酶活性的影响.大连海洋大学学报,27(5):422-428
郭勤单,王有基,吕为群,2014.温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响.水生生物学报,38(1):58-67
黄玉瑶,1988.青鳉的生物学特性与饲养管理技术.动物学杂志,23(6):28-31
崔前进,陈冰,邱丽华等,2017.低盐胁迫对钝吻黄盖鲽幼鱼鳃Na+/K+-ATP酶、肝脏抗氧化酶和非特异免疫酶的影响.广东海洋大学学报,(6):26-32
梁国栋,王辉,刘加慧等,2014.罗非鱼仔鱼开口率与温度、盐度间模型的建立与优化.广东海洋大学学报,34(4):33-34
廖雅丽,张晨捷,彭士明等,2016.盐度对云纹石斑鱼抗氧化酶及溶菌酶活性的影响.上海海洋大学学报,25(2):169-176
Abou Anni I S,Bianchini A,Barcarolli I F et al,2016.Salinity influence on growth,osmoregulation and energy turnover in juvenile pompano Trachinotus marginatus Cuvier 1832.Aquaculture,455:63-72
Bisazza A,Butterworth B,Piffer L et al,2014.Collective enhancement of numerical acuity by meritocratic leadership in fish.Scientific Reports,4:4560
Canzian J,Fontana B D,Quadros V A et al,2017.Conspecific alarm substance differently alters group behavior of zebrafish populations:Putative involvement of cholinergic and purinergic signaling in anxiety-and fear-like responses.Behavioural Brain Research,320:255-263
Chen X P,Li L,Wong C K et al,2009.Rapid adaptation of molecular resources from zebrafish and medaka to develop an estuarine/marine model.Comparative Biochemistry and Physiology Part C:Toxicology&Pharmacology,149(4):647-655
De Azevedo R V,Dos Santos-Costa K,De Oliveira K F et al,2015.Responses of Nile tilapia to different levels of water salinity.Latin American Journal of Aquatic Research,43(5):828-835
Foster S J,Vincent A C J,2004.Life history and ecology of seahorses:implications for conservation and management.Journal of Fish Biology,65(1):1-61
Gabillard J C,Weil C,Rescan P Y et al,2003.Effects of environmental temperature on IGF1,IGF2,and IGF type Ireceptor expression in rainbow trout(Oncorhynchus mykiss).General and Comparative Endocrinology,133(2):233-242
Handeland S O,Berge?,Bj?rnsson B T et al,2000.Seawater adaptation by out-of-season Atlantic salmon(Salmo salar L.)smolts at different temperatures.Aquaculture,181(3-4):377-396
Handeland S O,Imsland A K,Stefansson S O,2008.The effect of temperature and fish size on growth,feed intake,food conversion efficiency and stomach evacuation rate of Atlantic salmon post-smolts.Aquaculture,283(1-4):36-42
Hergenrader G L,Hasler A D,1967.Seasonal changes in swimming rates of Yellow Perch in lake Mendota as measured by sonar.Transactions of the American Fisheries Society,96(4):373-382
Huang Z H,Ma A J,Wang X A et al,2015.Interaction of temperature and salinity on the expression of immunity factors in different tissues of juvenile turbot Scophthalmus maximus based on response surface methodology.Chinese Journal of Oceanology and Limnology,33(1):28-36
Jain K E,Farrell A P,2003.Influence of seasonal temperature on the repeat swimming performance of rainbow trout Oncorhynchus mykiss.Journal of Experimental Biology,206:3569-3579
Jobling M,1993.Bioenergetics:Feed intake and energy partitioning.In:Rankin J C,Jensen F B eds.Fish Ecophysiology.Dordrecht:Springer
Keen J E,Farrell A P,1994.Maximum prolonged swimming speed and maximum cardiac performance of rainbow trout,Oncorhynchus mykiss,acclimated to two different water temperatures.Comparative Biochemistry and Physiology Part A:Physiology,108(2-3):287-295
Kinoshita M,Murata K,Naruse K et al,2009.Medaka:Biology,Management,and Experimental Protocols.Iowa City,IA,Iowa,USA:Wiley-Blackwell,9-11,23-24
Lee H B,Kim D S,Gil H W et al,2018.Pysiological responses of diploid and triploid far eastern catfish,Silurus asotus to water temperature stress.Development&Reproduction,22(2):165-174
Livingstone D R,2001.Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms.Marine Pollution Bulletin,42(8):656-666
Luo Y P,Xie X J,2008.Effects of temperature on the specific dynamic action of the southern catfish,Silurus meridionalis.Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology,149(2):150-156
Meyer H A,Chipps S R,Graeb B D S et al,2016.Growth,food consumption,and energy status of juvenile pallid sturgeon fed natural or artificial diets.Journal of Fish and Wildlife Management,7(2):389-396
Mork O I,Bjerkeng B,Rye M,1999.Aggressive interactions in pure and mixed groups of juvenile farmed and hatchery-reared wild Atlantic salmon Salmo salar L.in relation to tank substrate.Aquaculture Research,30(8):571-578
Mourente G,Díaz-Salvago E,Bell J G et al,2002.Increased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream(Sparus aurata L.)fed dietary oxidised oil:attenuation by dietary vitamin E.Aquaculture,214(1-4):343-361
Rambo C L,Mocelin R,Marcon M et al,2017.Gender differences in aggression and cortisol levels in zebra fish subjected to unpredictable chronic stress.Physiology and Behavior,171:50-54
Rome L C,Funke R P,Alexander R M,1990.The influence of temperature on muscle velocity and sustained performance in swimming carp.Journal of Experimental Biology,154:163-178
Russell N R,Fish J D,Wootton R J,1996.Feeding and growth of juvenile sea bass:the effect of ration and temperature on growth rate and efficiency.Journal of Fish Biology,49(2):206-220
Sergio C V,Jesús T P P,Gerónimo R C et al,2013.Effects of temperature and salinity on growth and survival of the Pacific red snapper Lutjanus peru(Pisces:Lutjanidae)juvenile.Latin American Journal of Aquatic Research,41(5):1013-1018
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