平卧菊三七各提取物抗痛风作用的实验研究
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摘要
目的:研究平卧菊三七各提取物的抗痛风作用。方法:腹腔注射尿酸酶抑制剂氧嗪酸钾建立小鼠高尿酸血症模型,灌胃给予平卧菊三七各提取物,用磷钨酸法检测动物血清中尿酸水平,评价平卧菊三七各提取物的降尿酸作用;腹腔注射冰醋酸,灌胃给予平卧菊三七各提取物,统计小鼠扭体次数,评价平卧菊三七各提取物的镇痛作用;直接涂抹二甲苯,致小鼠耳廓肿胀,灌胃给予平卧菊三七各提取物,计算小鼠耳肿胀度,评价平卧菊三七各提取物的抗炎作用。结果:4000mg生药/kg的平卧菊三七醇提物能显著性降低高尿酸血症小鼠血清尿酸水平,4000mg生药/kg的平卧菊三七水提物、醇提物和水提醇沉物以及8000mg生药/kg的平卧菊三七水提醇沉物能够显著减少冰醋酸刺激引起的扭体次数;4000mg生药/kg的平卧菊三七水提物、醇提物和水提醇沉物以及8000mg生药/kg的平卧菊三七水提醇沉物能够显著抑制二甲苯致小鼠耳肿胀。结论:4000mg生药/kg的平卧菊三七醇提物具有较强的抗痛风作用。
Objective: To investigate the anti-gout effect in each extraction from Gynura Procumbens. Methods: The hypouricemic effect of Gynura Procumbens in each extrac was investigated in hyperuricemia mice by Intraperitoneal injection the uricase inhibitor Potassium Oxonate,The mice were intragastric filled by Gynura Procumbens in each extrac,and the Uric Acid levels in mice was detected by phosphotungstic acid method. The method of acetic acid writhing was used to study the antinociceptive property of each extrac from Gynura Procumbens. the mice were intraperitoneal injection of acetic acid and intragastric filled by Gynura Procumbens in each extrac,and Collect the writhing times of mice,and the anti-inflammatory effect of Gynura Procumbens in each extrac was observed by the swelling of ear induced by dimethylbenzene directly in mice,The mice were intragastric filled by Gynura Procumbens in each extrac also,and the ear swelling in mice were calculated. Results: The results showed that 4000 mg crude drugs/kg of Gynura Procumbens alcohol precipitation can significantly reduced the serum urate levels in hyperuricemia mice; 4000 mg crude drugs/kg of Gynura Procumbens Water extract、alcohol precipitation、water extract and alcohol extract and 8000 mg crude drugs/kg of Gynura Procumbens water extract and alcohol extract can significantly reduced glacial acetic acid stimulus caused of twist body times; 4000 mg crude drugs/kg of Gynura Procumbens Water extract、alcohol precipitation,water extract and alcohol extract and 8000 mg crude drugs/kg of Gynura Procumbens alcohol precipitation can significantly inhibit xylene to small rat ear swelling. Conclusion: 4000 mg crude drugs/kg of Gynura Procumbens alcohol precipitation has anti-gout effects.
Objective: To investigate the anti-gout effect in each extraction from Gynura Procumbens. Methods: The hypouricemic effect of Gynura Procumbens in each extrac was investigated in hyperuricemia mice by Intraperitoneal injection the uricase inhibitor Potassium Oxonate,The mice were intragastric filled by Gynura Procumbens in each extrac,and the Uric Acid levels in mice was detected by phosphotungstic acid method. The method of acetic acid writhing was used to study the antinociceptive property of each extrac from Gynura Procumbens. the mice were intraperitoneal injection of acetic acid and intragastric filled by Gynura Procumbens in each extrac,and Collect the writhing times of mice,and the anti-inflammatory effect of Gynura Procumbens in each extrac was observed by the swelling of ear induced by dimethylbenzene directly in mice,The mice were intragastric filled by Gynura Procumbens in each extrac also,and the ear swelling in mice were calculated. Results: The results showed that 4000 mg crude drugs/kg of Gynura Procumbens alcohol precipitation can significantly reduced the serum urate levels in hyperuricemia mice; 4000 mg crude drugs/kg of Gynura Procumbens Water extract、alcohol precipitation、water extract and alcohol extract and 8000 mg crude drugs/kg of Gynura Procumbens water extract and alcohol extract can significantly reduced glacial acetic acid stimulus caused of twist body times; 4000 mg crude drugs/kg of Gynura Procumbens Water extract、alcohol precipitation,water extract and alcohol extract and 8000 mg crude drugs/kg of Gynura Procumbens alcohol precipitation can significantly inhibit xylene to small rat ear swelling. Conclusion: 4000 mg crude drugs/kg of Gynura Procumbens alcohol precipitation has anti-gout effects.
引文
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[10]Niu YF,Lu W,Gao LH,et al.Reducing effect of mangiferin on serum uric acid levels in mice[J].Pharmaceutical Biology,2012,50(9):1177-1182.
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[12]李蒙,沈佳瑜,李听弦,等.苍耳子炮制前后的抗炎、镇痛作用比较[J].中国医院药学杂志,2017,37(3):232-234.
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[14]王会,吴秀英,李长贵,等.痛风与脂代谢紊乱及肥胖的关系[J].山东医药,2012,52(5):10-12.
[15]杜玲.血尿酸与心脑血管疾病易患因素相关性分析[J].中国医院用药评价与分析,2016,16(12):1 723-1 725.
[16]Doherty M.New insights into the epidemiology of gout[J].Rheumatology(Oxford),2009,48(2):i2-i8.
[17]姜贵云,杨晓莲.痛风病的诊断评估与临床特征[J].中国临床康复,2006,10(28):150-155.
[18]刘秀婵,白人骁.痛风发病机制及药物治疗最新研究进展[J].河北医科大学学报,2016,37(9):1 108-1 112.
[19]金信良.四妙丸加味配合中药外敷治疗风湿热型痛风性关节炎168例[J].浙江中医杂志,2012,47(10):739-739.
[20]黄丽贞,杨玲玲,邓家刚,等.复方威茯颗粒抗痛风作用的实验研究[J].中药材,2016,39(1):188-192.
[21]余雄英,周军,曹树稳,等.茶树根抗痛风作用的实验研究[J].江西中医药,2014,45(382):36-38.
[2]Algariri K,Atangwho I J,Meng K Y,et al.antihy-perglycaemic and toxicological evaluations of extract andfractions of Gynura procumbens leaves[J].Trop Life Scires.2014,25(1):75-93.
[3]尹玉辉.药食兼用植物-平卧菊三七[J].农村百事通,2012,14:42-43.
[4]朱玉婷,廖为明,郑国栋,等.平卧菊三七中绿原酸提取及纯化工艺的优化[J].湖北农业科学,2012,51(19):4 349-4 351.
[5]Sriwanthana B,Treesangsri W,Boriboontrakul B,et al.In vitro effects of Thai medicinal plants on human lymphocyte activity[J].Songklanakarin J Sci Technol,2007,29(Sup 1):17-28.
[6]Li XJ,Mu YM,Li TT,et al.Gynura procumbens reverses acute and chronic ethanal-induced liver steatosis through MAPK/SREBP-1C-dependent and-independent pathways[J].J Agric Food Chem,2015,63(38):8 640-8 471.
[7]Tan HL,Chan KG,Pusparajah P,et al.Gynura procumbens:An Overview of the Biological Activities[J].Front Pharmacol,2016,7:52.
[8]Hew CS,Gam LH.Proteome analysis of abundant proteins extracted from the leaf of Gynura procumbens(Lour.)Merr[J].Appl Biochem Biotechnol,2011,165(7/8):1 577-1 586.
[9]姚亮亮,杨丽,万春鹏,等.菊三七属植物化学成分及药理活性研究进展[J].北方园艺,2016(24):195-200.
[10]Niu YF,Lu W,Gao LH,et al.Reducing effect of mangiferin on serum uric acid levels in mice[J].Pharmaceutical Biology,2012,50(9):1177-1182.
[11]陈奇,张伯礼.中药药效研究方法学[M].北京:人民卫生出版社,2016:484-503.
[12]李蒙,沈佳瑜,李听弦,等.苍耳子炮制前后的抗炎、镇痛作用比较[J].中国医院药学杂志,2017,37(3):232-234.
[13]Mazzali M,Hughes J,Kim YG,et al.Elevated uric acid increases blood pressure in the rat by a novel crystal-independent mechanism[J].Hypertension,2001,38(5):1 101-1 206.
[14]王会,吴秀英,李长贵,等.痛风与脂代谢紊乱及肥胖的关系[J].山东医药,2012,52(5):10-12.
[15]杜玲.血尿酸与心脑血管疾病易患因素相关性分析[J].中国医院用药评价与分析,2016,16(12):1 723-1 725.
[16]Doherty M.New insights into the epidemiology of gout[J].Rheumatology(Oxford),2009,48(2):i2-i8.
[17]姜贵云,杨晓莲.痛风病的诊断评估与临床特征[J].中国临床康复,2006,10(28):150-155.
[18]刘秀婵,白人骁.痛风发病机制及药物治疗最新研究进展[J].河北医科大学学报,2016,37(9):1 108-1 112.
[19]金信良.四妙丸加味配合中药外敷治疗风湿热型痛风性关节炎168例[J].浙江中医杂志,2012,47(10):739-739.
[20]黄丽贞,杨玲玲,邓家刚,等.复方威茯颗粒抗痛风作用的实验研究[J].中药材,2016,39(1):188-192.
[21]余雄英,周军,曹树稳,等.茶树根抗痛风作用的实验研究[J].江西中医药,2014,45(382):36-38.