七味白术散加蔗糖对菌群失调腹泻肠道乳酸杆菌的影响
摘要
目的探讨蔗糖对七味白术散治疗菌群失调腹泻肠道乳酸杆菌多样性的影响,为蔗糖对七味白术散疗效的影响提供依据,指导服用七味白术散时,科学选择矫味剂。方法应用抗生素制备菌群失调腹泻小鼠,通过50%量超微七味白术散汤剂、50%量超微七味白术散汤剂加蔗糖、七味白术散传统汤剂灌胃治疗。治疗结束后,提取肠道内容物,选用特定引物对肠道乳酸菌扩增后进行多样性分析。结果 50%量超微七味白术散汤剂加蔗糖组OTUs数4低于正常组和50%量超微七味白术散汤剂组;通过聚类分析和主成分分析得知50%量超微七味白术散汤剂组比50%量超微七味白术散汤剂加蔗糖组更接近正常组。说明50%量超微七味白术散汤剂组与50%量超微七味白术散汤剂加糖组均能治疗小鼠菌群失调腹泻,50%量超微七味白术散汤剂组对治疗菌群失调腹泻小鼠乳酸杆菌多样性的影响优于50%量超微七味白术散汤剂加糖组。结论蔗糖对七味白术散的疗效有一定影响,临床用药时,最好不添加蔗糖,关于最佳矫味剂,有待进一步实验研究。
引文
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[5]PENG Xinxin.Effecte of sucrose on intestinal microorganisms andenzymes in diarrhea mice[D],Journal of Hunan Uniwersity of Traditional Chinese Medicine,2015.
[6] YU XW.Pediatric diarrhea-qiweibaizhusan[J].Family medicine,2007,(6):25.
[7]TAN Zhoujin ,WU Hai,LIU FuLin,et al.Effect of ultra-micro powder Qiweibaizhusan on the intestinal microbiota and enzymeactivities in mice [J].Acta Ecol Sin,2012,32 (21):6856-6863.
[8]ZHANG Silu,LIU Xiaoyun,ZHANG Haoqi,etal Development of a genus- specific T-RFLP technique for differentiating Lacttobacillus community[J].Microbiology China,2012,39(8):1179-1189.
[9] ZENG Ao,ZHANG Hualing,TAN Zhoujin et al.The construction of mice diarrhea model due to dysbacteriosis and curative effect of ultra-micro Qiweibaizhusan[J].Microbiology China..2012,39(9):1341-1348.
[10]WU Hai .Effect of ultra-Qiweibaizhusan on microbial molecular diversity of intestinal[D].Hunan Uniwersity of Traditional Chinese Medicine.2012.
[11] WU Hai,ZHOU Sainan,GUO Chun,et al.A Method for the Extraction of Intestinal Microorganisms in Mice by Genome Based on PCR Analysis[J],Chinese Journal of Microecology2012,24(7):648-651.
[12] LI Yang,ZHANG Tiejun,LIU Suxiang,et al.Adxances in chenmical canstituents and pharmacology[J].Chinese herbal medicine.
[13] PENG Xinxin.Effecte of sucrose on intestinal microorganisms andenzymes in diarrhea mice[D],Journal of Hunan Uniwersity of Traditional Chinese Medicine,2015.
[14]LONG Chengxing,LIU Youjia,DENG Tianhao,et al.The influence of sucrose on the efficacy of Qiwei Baizhu powder cambined with yeast in treatment of dysbacteriotic diarrhea in mice[J].Chinese Journal of Microecology,2015,27(11):1241-1249.
[15] PU Junan,ZHENG Tao,LI Can,et al.Effect of sucrose on Qiweibaizhusan to intestinal bacteria diversity dysbacterotic diarrhea mice[J].China Academic Journal Electronic Publishing House,2017,36(6):583-589.
[15]SHAO Liu,XU Zuxin,YIN Hailong.Reseach progress of cabon source in denitrification process of polluted water[J].Industral Water Treament ,2007,27(12):12-13.
[16] Rodriguez S,Santamaria RI,Fernandez-Abalos JM,et al.Identification of the sequences involved in the glucose-repressed transcription of the Streptomyces halstedii JM8 xysA promoter[J].Gene ,2005,351:1-9.
[17] LIU Wei,YAO Wen ,ZHU Weiyun,reciprocal symbiotic relationship between human body and intestinal microbes[J].Chinese journal of digestology,2006,14(11):1084.
[18]Min C,Xu CD,Chen SN.Disaccharidase deficiency.Foreign Medical Sciences Section of Pediatrics[M] 2003:179-180.
[19] WANG Xiaoling,PENG Dingxiang.Effects of light abd temperature conditions and carbon source on callus growth and differentiation of ramie[J].Chinese agricultural science,2004,20(2):4.
[20] XIAO Xinyun ,LIU Youjia ,DENG Yanling et al.The Effect of Sucrose on Blood Routine of Dysbacteriosis Diarrheal Mice Treated with Qiweibaizhu powder[J].JOURNAL OF JIANGXI UNIVERSITY OF TCM,2017,29 (1):76-79.