遮阴条件下冬小麦材料生长发育特性相关基因分子鉴定
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摘要
【目的】研究耐阴小麦品种及种质资源遗传特性,对南疆主栽品种、部分新育成品种、国内引进品种,自育品系等材料以1Bl/1RS,春化基因,光周期基因,产量相关基因开展分子标记鉴定。了解现有材料的遗传背景,为选育适合新疆南疆"果麦间作"模式小麦新品种奠定基础。【方法】利用1Bl/1RS、Vrn-A1、Ppd-D1、Ta Sus2、Ta Cwi-A1a b(CW121) Ta Cwi-A1b(CW122)分子标记,对遮阴条件下的冬小麦材料主要生长发育特性基因进行分子鉴定分析。【结果】在鉴定70份冬麦材料中、1Bl/1RS(ω-sec-p1-p2)(ω-sec-p3-p4)类型为57. 1%、100%; Vrn-A1(Vrn-A1c)(vrn-A1)类型为97. 1%、74. 2%; Ppd-D1类型为100%; Ta Sus2(Hap-H)(Hap-L)类型为41. 4%、30%; CW121、CW122类型为50%、75. 7%;在4份主栽及部分育成品种、66份其他国内引进冬麦材料中,1Bl/1RS(sec-p1-p2)(sec-p3-p4)类型分别为25%、59%、100%、100%;Vrn-A1(Vrn-A1c)(vrn-A1)类型分别为75%、98. 4%、100%、72. 7%; Ppd-D1类型同为100%;主栽品种和新育成品种中未检测出Ta Sus2(Hap-H)类型,Ta Sus2(Hap-L)类型的比例为75%;其他国内引进品种TaSus2(Hap-H)(Hap-L)的比例为分别为43. 9%、27. 2%,南疆主栽和部分育成品种中CW121、CW122类型的分布频率75%、75%,其他国内引进品种中CW121、CW122类型分别为48. 4%、75. 7%(图1)。【结论】70份材料中的全部含有1BL/1RS (sec-p3-p4)易位系及对光周期非敏感性类型。4份主栽品和新育成主栽品种中含隐性春化基因,68份材料同时携带光周期非敏感性及显性春化基因。高千粒重相关基因类型的分布率均相当高(≥75%)。
【Objective】In order to further study the genetic characteristics of shade-tolerant wheat varieties and germplasm resources,the main cultivars in southern Xinjiang,some newly bred varieties,domestic introduced varieties,self-breeding lines,etc.,were selected as 1bl/1RS,vernalization gene and photoperiod gene for molecular marker identification. The aim was to understand the genetic background of the existing materials and lay the foundation for breeding new wheat varieties suitable for " fruit-wheat intercropping" model in southern Xinjiang. 【Method】The genes of main growth and development characteristics of winter wheat under shaded conditions were identified and analyzed by molecular markers 1Bl/1RS,Vrn-A1,Ppd-D1,TaSus2,TaCwi-A1a( CW121) and TaCwi-A1b( CW122).【Result】The results showed that of the 70 winter wheat materials identified,1Bl/1RS( ω-sec-p1-p2) and( ω-sec-p3-p4) were 57. 1% and100%; Vrn-A1( Vrn-A1c) and( vrn-A1) were 97. 1% and 74. 2%; Ppd-D1 gene type was 100%;TaSus2( Hap-H) and( Hap-L) were 41. 4% and 30%;; CW121 and CW122( Table1) were 50% and 75.7%( see Table 1); 1Bl/1RS( ω-sec-p1-p2)( ω-sec-p3-p4) types were 25%,59%,100%,100%,respectively among the four main newly bred variety cultivars,some bred varieties and 66 other domestic introduced winter wheat varieties,their gen type Vrn-A1,( Vrn-A1c) and( vrn-A1) accounting for75%,98. 4%,100% and 72. 7%,respectively; Ppd-D1 was 100%; TaSus2( Hap-H) was not detected in main and newly bred varieties. The proportion of tasus2( Hap-L) type was 75%; The percentage of other domestic introduced varieties TaSus2( Hap-H)( Hap-L) was 43. 9% and 27. 2%,respectively; The distribution frequency of CW121 and CW 122 type was 75% and 75% in the main and partially bred varieties in southern Xinjiang,and 48. 4% and 75. 7% in the other imported varieties in China( See fig. 1).【Conclusion】All materials of 70 materials were gene type contain 1Bl/1RS( sec-p3-p4) and non-photoperiod sensitivity. The recessive vernalization gene was found in 4 main cultivars and newly bred major cultivars,and68 materials carried photoperiod insensitive and dominant vernalization genes simultaneously. The distribution rates of gene types associated with 1,000-grain weight was fairly high( ≥75%).
【Objective】In order to further study the genetic characteristics of shade-tolerant wheat varieties and germplasm resources,the main cultivars in southern Xinjiang,some newly bred varieties,domestic introduced varieties,self-breeding lines,etc.,were selected as 1bl/1RS,vernalization gene and photoperiod gene for molecular marker identification. The aim was to understand the genetic background of the existing materials and lay the foundation for breeding new wheat varieties suitable for " fruit-wheat intercropping" model in southern Xinjiang. 【Method】The genes of main growth and development characteristics of winter wheat under shaded conditions were identified and analyzed by molecular markers 1Bl/1RS,Vrn-A1,Ppd-D1,TaSus2,TaCwi-A1a( CW121) and TaCwi-A1b( CW122).【Result】The results showed that of the 70 winter wheat materials identified,1Bl/1RS( ω-sec-p1-p2) and( ω-sec-p3-p4) were 57. 1% and100%; Vrn-A1( Vrn-A1c) and( vrn-A1) were 97. 1% and 74. 2%; Ppd-D1 gene type was 100%;TaSus2( Hap-H) and( Hap-L) were 41. 4% and 30%;; CW121 and CW122( Table1) were 50% and 75.7%( see Table 1); 1Bl/1RS( ω-sec-p1-p2)( ω-sec-p3-p4) types were 25%,59%,100%,100%,respectively among the four main newly bred variety cultivars,some bred varieties and 66 other domestic introduced winter wheat varieties,their gen type Vrn-A1,( Vrn-A1c) and( vrn-A1) accounting for75%,98. 4%,100% and 72. 7%,respectively; Ppd-D1 was 100%; TaSus2( Hap-H) was not detected in main and newly bred varieties. The proportion of tasus2( Hap-L) type was 75%; The percentage of other domestic introduced varieties TaSus2( Hap-H)( Hap-L) was 43. 9% and 27. 2%,respectively; The distribution frequency of CW121 and CW 122 type was 75% and 75% in the main and partially bred varieties in southern Xinjiang,and 48. 4% and 75. 7% in the other imported varieties in China( See fig. 1).【Conclusion】All materials of 70 materials were gene type contain 1Bl/1RS( sec-p3-p4) and non-photoperiod sensitivity. The recessive vernalization gene was found in 4 main cultivars and newly bred major cultivars,and68 materials carried photoperiod insensitive and dominant vernalization genes simultaneously. The distribution rates of gene types associated with 1,000-grain weight was fairly high( ≥75%).
引文
[1]方升佐,黄宝龙,徐锡增.高效杨树人工林复合经营体系的构建与应用[J].西南林学院学报,2005,(4):36-41.Fang Sheng-zuo,Huang Bao-long,Xu Xi-zeng.(2005). Establishment and potential application for effective agroforestrysystem of populus plantations. J Southwest For Coll,25(4):36-41.(in Chinese)
[2]于成景,方升佐,罗诚彬.杨树人工林林麦间作研究初报[J].林业科技开发,2007,(2):47-51.Yu Chen-jing,Fang Sheng-zuo,Luo Chen-bin.(2007). Apreliminary study on poplar-wheat intercropping patterns. China For Sci Technol,21(2):47-51.(in Chinese)
[3]YU Xiang.(2003). Effect on wheat yield and the quality due to variations of ecological factors by poplar intercropping with wheat.Journal of Anhui Agricultural Sciences,31(5):882-883.
[4]杜长恒,范家骅,刘俊秀,等.小麦熟期与产量构成因素的关系及育种途径的探讨[J].北京农业科学,1991,(4):1-5.DU Chang-heng,FAN Jia-hua,LIU Jun-xiu.(1991). Relationship between Maturity Period and Yield Components of Wheat and Approaches to Breeding[J]. Beijing Agricultural Sciences,(4):1-5.(in Chinese)
[5]周广生,梅方竹,陈艳华.冬小麦根系活力与产量形状关系的研究[J].华中农业大学学报,2001,(6):531-534ZHOU Guang-sheng,MEI Fang-zhu,CHEN Yan-hua.(2001). Study on the Relation between Root Activity and Yield Shape of Winter Wheat[J]. Journal of Huazhong Agricultural University,(6):531-534.(in Chinese)
[6]袁秀云.普通小麦春化基因的克隆及表达特性分析[D].郑州:河南农业大学硕士论文,2009.YUAN Xiu-yun.(2009). Cloning and Expression Characterization of Vernalization Gene in Common Wheat[D]. Master Dissertation. Henan Agricultural University,Zhengzhou.(in Chinese)
[7]赵彦坤,张文胜,王秀堂,等.黄淮北片主栽小麦品种冬春性与主要春化基因组成的关系[J].麦类作物学报,2016,36(11):1440-1 448.ZHAO Yan kun,ZHANG Wen sheng,WANG Xiu tang,et al.(2016). Analysis of winterness-springness type and vernalization Genes in the main wheat cultivars from the north of yellow and Huai valley of China[J]. Journal of Triticea Crops,36(11):1,440-1,448.(in Chinese)
[8]袁秀云,李永春,闫延涛,等.小麦春化发育的分子调控机理研究进展[J].西北植物学报,2008,28(7):1 486.YUAN Xiu-yun,LI Yong-chun,YAN Yan-tao,et al.(2008). Molecular regulatory mechanisms of vernalization in wheat[J]. Acta Botanica Bo-reali-Occidentaliasinica,28(7):1,486.(in Chinese)
[9]尹钧,任江萍,潘登奎,等.小麦春化发育相关蛋白质同工酶的研究[J].麦类作物学报,2002,22(1):33.YIN Jun,REN Jiang-ping,PAN Deng-kui,et al.(2002).The protein and isoenzymes related to vernalization of wheat[J].Journal of Triticeae Crops,22(1):33.(in Chinese)
[10]Hu,X.,Kong,X.,Wang,C.,Ma,L.,Zhao,J.,&Wei,J.,et al.(2014). Proteasome-mediated degradation of frigida modulates flowering time in arabidopsis during vernalization. Plant Cell,26(12):4,763-4,781.
[11]Xiao,J.,Xu,S.,Li,C.,Xu,Y.,Xing,L.,&Niu,Y.,et al.(2014). O-GIc NAc-mediated interaction between ver2and tagrp2 elicits tavrn1 mRNA accumulation during vernalization in winter wheat. Nature Communications,5(2):4,572.
[12]Kippes,N.,Zhu,J.,Chen,A.,Vanzetti,L.,&Dubcovsky,J.(2013). Fine mapping and epistatic interactions of the vernalization gene vrn-d4 in hexaploid wheat. MGG Molecular&General Genetics,289(1):47.
[13]赵虹,胡卫国,詹克慧,等.黄淮南片冬麦区主导品种春化基因及冬春性分析[J].西北植物学报,2010,30(3):495.ZHAO Hong,HU Wei-guo,ZHAN Ke-hui,et al.(2010).Analysis on vernalization alleles and winter-spring characteristic of wheat cultivars from the south of yellow and Huai River valley winter wheat zone[J]. Acta Botanica Boreali Occidentaliasinica,30(3):495.(in Chinese)
[2]于成景,方升佐,罗诚彬.杨树人工林林麦间作研究初报[J].林业科技开发,2007,(2):47-51.Yu Chen-jing,Fang Sheng-zuo,Luo Chen-bin.(2007). Apreliminary study on poplar-wheat intercropping patterns. China For Sci Technol,21(2):47-51.(in Chinese)
[3]YU Xiang.(2003). Effect on wheat yield and the quality due to variations of ecological factors by poplar intercropping with wheat.Journal of Anhui Agricultural Sciences,31(5):882-883.
[4]杜长恒,范家骅,刘俊秀,等.小麦熟期与产量构成因素的关系及育种途径的探讨[J].北京农业科学,1991,(4):1-5.DU Chang-heng,FAN Jia-hua,LIU Jun-xiu.(1991). Relationship between Maturity Period and Yield Components of Wheat and Approaches to Breeding[J]. Beijing Agricultural Sciences,(4):1-5.(in Chinese)
[5]周广生,梅方竹,陈艳华.冬小麦根系活力与产量形状关系的研究[J].华中农业大学学报,2001,(6):531-534ZHOU Guang-sheng,MEI Fang-zhu,CHEN Yan-hua.(2001). Study on the Relation between Root Activity and Yield Shape of Winter Wheat[J]. Journal of Huazhong Agricultural University,(6):531-534.(in Chinese)
[6]袁秀云.普通小麦春化基因的克隆及表达特性分析[D].郑州:河南农业大学硕士论文,2009.YUAN Xiu-yun.(2009). Cloning and Expression Characterization of Vernalization Gene in Common Wheat[D]. Master Dissertation. Henan Agricultural University,Zhengzhou.(in Chinese)
[7]赵彦坤,张文胜,王秀堂,等.黄淮北片主栽小麦品种冬春性与主要春化基因组成的关系[J].麦类作物学报,2016,36(11):1440-1 448.ZHAO Yan kun,ZHANG Wen sheng,WANG Xiu tang,et al.(2016). Analysis of winterness-springness type and vernalization Genes in the main wheat cultivars from the north of yellow and Huai valley of China[J]. Journal of Triticea Crops,36(11):1,440-1,448.(in Chinese)
[8]袁秀云,李永春,闫延涛,等.小麦春化发育的分子调控机理研究进展[J].西北植物学报,2008,28(7):1 486.YUAN Xiu-yun,LI Yong-chun,YAN Yan-tao,et al.(2008). Molecular regulatory mechanisms of vernalization in wheat[J]. Acta Botanica Bo-reali-Occidentaliasinica,28(7):1,486.(in Chinese)
[9]尹钧,任江萍,潘登奎,等.小麦春化发育相关蛋白质同工酶的研究[J].麦类作物学报,2002,22(1):33.YIN Jun,REN Jiang-ping,PAN Deng-kui,et al.(2002).The protein and isoenzymes related to vernalization of wheat[J].Journal of Triticeae Crops,22(1):33.(in Chinese)
[10]Hu,X.,Kong,X.,Wang,C.,Ma,L.,Zhao,J.,&Wei,J.,et al.(2014). Proteasome-mediated degradation of frigida modulates flowering time in arabidopsis during vernalization. Plant Cell,26(12):4,763-4,781.
[11]Xiao,J.,Xu,S.,Li,C.,Xu,Y.,Xing,L.,&Niu,Y.,et al.(2014). O-GIc NAc-mediated interaction between ver2and tagrp2 elicits tavrn1 mRNA accumulation during vernalization in winter wheat. Nature Communications,5(2):4,572.
[12]Kippes,N.,Zhu,J.,Chen,A.,Vanzetti,L.,&Dubcovsky,J.(2013). Fine mapping and epistatic interactions of the vernalization gene vrn-d4 in hexaploid wheat. MGG Molecular&General Genetics,289(1):47.
[13]赵虹,胡卫国,詹克慧,等.黄淮南片冬麦区主导品种春化基因及冬春性分析[J].西北植物学报,2010,30(3):495.ZHAO Hong,HU Wei-guo,ZHAN Ke-hui,et al.(2010).Analysis on vernalization alleles and winter-spring characteristic of wheat cultivars from the south of yellow and Huai River valley winter wheat zone[J]. Acta Botanica Boreali Occidentaliasinica,30(3):495.(in Chinese)