从生长年限和入药部位探讨厚朴净制的意义
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摘要
目的:研究厚朴不同生长年限、不同入药部位净制去粗皮的科学性。方法:采用高效液相色谱法,测定已知不同生长年限(10,15,20,25,30,40年)和市售未知年限的不同部位(干皮、枝皮、根皮)的带皮厚朴、去皮厚朴、厚朴粗皮中有效成分厚朴酚与和厚朴酚的含量。结果:已知不同生长年限、不同药用部位的厚朴净制前后两种酚类成分含量均为去皮厚朴>带皮厚朴>厚朴粗皮;枝皮、根皮部位粗皮所占比例分别为5.09%-6.66%、4.42%-10.84%;生长年限在10-25年间的厚朴,其干皮粗皮所占比例为10%以下,30-40年干皮粗皮所占比例增长为20%左右;根皮中两种酚类含量显著高于枝皮、干皮;未知年限、不同部位的厚朴净制后两种酚类成分含量变化规律与已知生长年限一致。结论:不同年限、不同部位的厚朴净制后两种酚类含量均升高,表明粗皮为厚朴中的非药用部位,厚朴净制具有一定的科学性;厚朴粗皮所占比例与其生长年限有关;厚朴不同部位酚类成分含量差异较大,建议厚朴应按部位不同区分等级以保证临床药效;厚朴净制是否去粗皮单从其酚类含量等方面确定似有不足,还应结合对其他成分的影响以及药效等多方面综合考虑。
Objective: To study the scientificity of Magnolia officinalis cleansing by different growth years and different medical parts. Methods: The contents of magnolol and honokiol in different parts(trunk bark, branch bark, root bark) of known growth years(10, 15, 20, 25, 30, 40 years) and unknown years Magnolia officinalis with peels, peeled Magnolia officinalis and rough leather of Magnolia officinalis were determined by high performance liquid chromatography.Results: The two phenolic contents of different known growth years of Magnolia officinalis cleansing in different parts was: peeled Magnolia officinalis> Magnolia Officinalis with peels> rough barks of Magnolia Officinalis. The ratio of medicinal materials in branches and roots was 5.09%-6.66% and 4.42%-10.84% respectively. The proportion of dry and rough bark of Magnolia which has a growth period of 10 to 25 years is below 10%, and the proportion of dry and rough bark of Magnolia which has a growth period of 30 to 40 years increased by about 20%. The two phenolic contents in the root bark was significantly higher than that of the branch bark and trunk bark. The changes of the content of two phenolic components in different parts of the unknown growth years were consistent with the known growth years. Conclusion: The phenolic content increases in different years and different parts of Magnolia officinalis after cleansing, which means rough bark was a non-medicinal part of Magnolia officinalis indicating that cleansing has a certain degree of scientificity.The proportion of rough bark in Magnolia officinalis was related to its growth period. The content of phenolic components in different parts of Magnolia officinalis was quite different. It is recommended that Magnolia officinalis should be graded according to different parts to ensure clinical efficacy. Whether or not the Magnolia officinalis was net to the rough bark was determined to be insufficient in terms of its phenolic content, and should be considered in combination with the effects of other ingredients and the efficacy.
Objective: To study the scientificity of Magnolia officinalis cleansing by different growth years and different medical parts. Methods: The contents of magnolol and honokiol in different parts(trunk bark, branch bark, root bark) of known growth years(10, 15, 20, 25, 30, 40 years) and unknown years Magnolia officinalis with peels, peeled Magnolia officinalis and rough leather of Magnolia officinalis were determined by high performance liquid chromatography.Results: The two phenolic contents of different known growth years of Magnolia officinalis cleansing in different parts was: peeled Magnolia officinalis> Magnolia Officinalis with peels> rough barks of Magnolia Officinalis. The ratio of medicinal materials in branches and roots was 5.09%-6.66% and 4.42%-10.84% respectively. The proportion of dry and rough bark of Magnolia which has a growth period of 10 to 25 years is below 10%, and the proportion of dry and rough bark of Magnolia which has a growth period of 30 to 40 years increased by about 20%. The two phenolic contents in the root bark was significantly higher than that of the branch bark and trunk bark. The changes of the content of two phenolic components in different parts of the unknown growth years were consistent with the known growth years. Conclusion: The phenolic content increases in different years and different parts of Magnolia officinalis after cleansing, which means rough bark was a non-medicinal part of Magnolia officinalis indicating that cleansing has a certain degree of scientificity.The proportion of rough bark in Magnolia officinalis was related to its growth period. The content of phenolic components in different parts of Magnolia officinalis was quite different. It is recommended that Magnolia officinalis should be graded according to different parts to ensure clinical efficacy. Whether or not the Magnolia officinalis was net to the rough bark was determined to be insufficient in terms of its phenolic content, and should be considered in combination with the effects of other ingredients and the efficacy.
引文
1汉张仲景.金匮玉函经.北京:北京科学技术出版社, 2016:83.
2 龚千锋.中药炮制学.北京:中国中医药出版社, 2012:81.
3 清黄奭.神农本草经.北京:中医古籍出版社, 1987:212.
4 国家药典委员会.中华人民共和国药典一部.北京:中国医药科技出版社, 2015:251-252.
5 王智鹏.姜炙厚朴的相关研究.成都:成都中医药大学硕士研究生学位论文, 2011.
6 冯瑾,李继遥,周学东.厚朴活性成分对致龋菌生长和产酸影响的体外研究.四川大学学报(医学版), 2007(3):456-458.
7 李杰萍,梁统,周克元.厚朴酚对趋化三肽激活的大鼠中性粒细胞功能的影响.中国药科大学学报, 2003(3):66-69.
8[日]池田浩治.厚朴酚抑制肿瘤细胞增殖.国外医学(中医中药分册),2002, 24(4):248-250.
9 张勇,唐方.厚朴酚药理作用的最新研究进展.中国中药杂志, 2012,37(23):3526-3530.
10 宋刘景源.太平惠民和剂局方(卷上).北京:人民卫生出版社,2017:371.
11 明李梴.医学入门.北京:中国医药科技出版社, 2011:147.
12 王家骅,任玉基.中药去皮壳的合理性探讨.基层中药杂志, 1992(1):12-13.
13 杨红兵,詹亚华,陈科力,等.发汗与去皮对厚朴中酚类成分含量的影响.中药材, 2007(1):22-23.
14 王智鹏,胡昌江,帅小翠,等.厚朴“去皮”的净制研究.时珍国医国药, 2011, 22(10):2573-2574.
15 张丹,颜学伟,曹纬国,等.重庆产厚朴不同部位厚朴酚与和厚朴酚分布规律的研究.中国现代应用药学, 2012, 29(7):614-617.
16 宋寇宗奭.本草衍义.中国医药科技出版社, 2012:56.
2 龚千锋.中药炮制学.北京:中国中医药出版社, 2012:81.
3 清黄奭.神农本草经.北京:中医古籍出版社, 1987:212.
4 国家药典委员会.中华人民共和国药典一部.北京:中国医药科技出版社, 2015:251-252.
5 王智鹏.姜炙厚朴的相关研究.成都:成都中医药大学硕士研究生学位论文, 2011.
6 冯瑾,李继遥,周学东.厚朴活性成分对致龋菌生长和产酸影响的体外研究.四川大学学报(医学版), 2007(3):456-458.
7 李杰萍,梁统,周克元.厚朴酚对趋化三肽激活的大鼠中性粒细胞功能的影响.中国药科大学学报, 2003(3):66-69.
8[日]池田浩治.厚朴酚抑制肿瘤细胞增殖.国外医学(中医中药分册),2002, 24(4):248-250.
9 张勇,唐方.厚朴酚药理作用的最新研究进展.中国中药杂志, 2012,37(23):3526-3530.
10 宋刘景源.太平惠民和剂局方(卷上).北京:人民卫生出版社,2017:371.
11 明李梴.医学入门.北京:中国医药科技出版社, 2011:147.
12 王家骅,任玉基.中药去皮壳的合理性探讨.基层中药杂志, 1992(1):12-13.
13 杨红兵,詹亚华,陈科力,等.发汗与去皮对厚朴中酚类成分含量的影响.中药材, 2007(1):22-23.
14 王智鹏,胡昌江,帅小翠,等.厚朴“去皮”的净制研究.时珍国医国药, 2011, 22(10):2573-2574.
15 张丹,颜学伟,曹纬国,等.重庆产厚朴不同部位厚朴酚与和厚朴酚分布规律的研究.中国现代应用药学, 2012, 29(7):614-617.
16 宋寇宗奭.本草衍义.中国医药科技出版社, 2012:56.