发酵饲料对生长育肥猪结肠微生物发酵及菌群组成的影响
|
摘要
【目的】研究复合菌发酵饲料对生长育肥猪结肠发酵、结肠黏膜与结肠内容物菌群组成的影响。【方法】采用气相色谱法检测育肥猪结肠内容物中挥发性脂肪酸浓度;采用MiSeq高通量测序方法检测育肥猪结肠黏膜与内容物中细菌菌群组成。【结果】饲喂发酵饲料对结肠黏膜及内容物中菌群多样性无显著影响(P>0.05);显著提高了猪结肠黏膜中魏斯菌属和柔嫩梭菌属的相对丰度(P<0.05),提高了结肠内容物中魏斯菌属、Subdoligranulum菌属相对丰度(P<0.05);饲喂发酵饲料对结肠内容物中pH、乳酸、乙酸、丙酸、异丁酸、戊酸、异戊酸和总挥发性脂肪酸浓度无显著影响(P>0.05),但显著提高了结肠内容物中的丁酸水平(P<0.05)。【结论】饲喂复合菌发酵饲料可在一定程度上影响育肥猪结肠中细菌菌群的组成,促进丁酸生成,对肠道健康具有改善作用。
[Objective] This study was to investigate the effect of a fermented feed on colonic fermentation and microflora of finishing pigs. [Methods] Capillary column gas chromatography was used to measure volatile fatty acid(VFA) concentration. Illumina MiSeq platform was used to detect community structure and diversity of the microflora of colonic mucosa and digesta. [Results] Fermented feed had no significant effect on microbial diversity of colonic mucosa and digesta(P>0.05), while increased the relative abundance of Weissella and Faecalibacteriumin colonic mucosa(P<0.05). The relative abundance of Weissella and Subdoligranulum was higher in colonic digesta(P<0.05) compared with the control group. No significant difference was observed in pH, lactate, acetate, propionate, isobutyrate, valerate, isovalerate and total VFA concentration between the two groups(P>0.05), whereas butyrate concentration was greater in the treatment(P<0.05). [Conclusion] Fermented feed could affect the composition of colonic microflora and promote the formation of colonic butyric acid in finishing pigs, which could improve their intestinal health.
[Objective] This study was to investigate the effect of a fermented feed on colonic fermentation and microflora of finishing pigs. [Methods] Capillary column gas chromatography was used to measure volatile fatty acid(VFA) concentration. Illumina MiSeq platform was used to detect community structure and diversity of the microflora of colonic mucosa and digesta. [Results] Fermented feed had no significant effect on microbial diversity of colonic mucosa and digesta(P>0.05), while increased the relative abundance of Weissella and Faecalibacteriumin colonic mucosa(P<0.05). The relative abundance of Weissella and Subdoligranulum was higher in colonic digesta(P<0.05) compared with the control group. No significant difference was observed in pH, lactate, acetate, propionate, isobutyrate, valerate, isovalerate and total VFA concentration between the two groups(P>0.05), whereas butyrate concentration was greater in the treatment(P<0.05). [Conclusion] Fermented feed could affect the composition of colonic microflora and promote the formation of colonic butyric acid in finishing pigs, which could improve their intestinal health.
引文
[1]Li YK,Mao SY,Zhu WY.Current situation in research and application of fermented feed with probiotics.Animal Husbandry&Veterinary Medicine,2009,41(3):90-93.(in Chinese)李永凯,毛胜勇,朱伟云.益生菌发酵饲料研究及应用现状.畜牧与兽医,2009,41(3):90-93.
[2]Wang C,Shi CY,Zhang Y,Song DG,Lu ZQ,Wang YZ.Microbiota in fermented feed and swine gut.Applied Microbiology and Biotechnology,2018,102(7):2941-2948.
[3]Canibe N,Jensen BB.Fermented and nonfermented liquid feed to growing pigs:effect on aspects of gastrointestinal ecology and growth performance.Journal of Animal Science,2003,81(8):2019-2031.
[4]Qin WL.Determination of rumen volatile fatty acids by means of gas chromatography.Journal of Nanjing Agricultural University,1982,5(4):110-116.(in Chinese)秦为琳.应用气相色谱测定瘤胃挥发性脂肪酸方法的研究改进.南京农业大学学报,1982,5(4):110-116.
[5]Khafipour E,Li SC,Plaizier JC,Krause DO.Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis.Applied and Environmental Microbiology,2009,75(22):7115-7124.
[6]Kuroiwa T,Iwanaga M,Kobari K,Higashionna A,Kinjyo F,Saito A.Preventive effect of Clostridium butyricum M588against the proliferation of Clostridium difficile during antimicrobial therapy.The Journal of the Japanese Association for Infectious Diseases,1990,64(11):1425-1432.
[7]Venkatraman A,Ramakrishna BS,Shaji RV,Kumar NSN,Pulimood A,Patra S.Amelioration of dextran sulfate colitis by butyrate:role of heat shock protein 70 and NF-κB.American Journal of Physiology-Gastrointestinal and Liver Physiology,2003,285(1):G177-G184.
[8]Kinoshita M,Suzuki Y,Saito Y.Butyrate reduces colonic paracellular permeability by enhancing PPARγactivation.Biochemical and Biophysical Research Communications,2002,293(2):827-831.
[9]Van Der Meulen R,Makras L,Verbrugghe K,Adriany T,De Vuyst L.In vitro kinetic analysis of oligofructose consumption by Bacteroides and Bifidobacterium spp.indicates different degradation mechanisms.Applied and Environmental Microbiology,2006,72(2):1006-1012.
[10]Ray AK,Roy T,Mondal S,Ring?E.Identification of gut-associated amylase,cellulase and protease‐producing bacteria in three species of Indian major carps.Aquaculture Research,2010,41(10):1462-1469.
[11]Lamendella R,Domingo JWS,Ghosh S,Martinson J,Oerther DB.Comparative fecal metagenomics unveils unique functional capacity of the swine gut.BMC Microbiology,2011,11(1):103-119.
[12]Ohland CL,Mac Naughton WK.Probiotic bacteria and intestinal epithelial barrier function.American Journal of Physiology-Gastrointestinal and Liver Physiology,2010,298(6):G807-G819.
[13]Ashida H,Ogawa M,Kim M,Mimuro H,Sasakawa C.Bacteria and host interactions in the gut epithelial barrier.Nature Chemical Biology,2012,8(1):36-45.
[14]Patterson MF,Mc Kay AM,Connolly M,Linton M.Effect of high pressure on the microbiological quality of cooked chicken during storage at normal and abuse refrigeration temperatures.Food Microbiology,2010,27(2):266-273.
[15]Tingirikari JMR,Kothari D,Goyal A.Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications.Food&Function,2014,5(9):2324-2330.
[16]Feng J,Shi QS,Ouyang YS,et al.Advance in classification and main application of Gluconobacter.Journal of Microbiology,2010,30(2):86-90.(in Chinese)冯静,施庆珊,欧阳友生,等.葡糖杆菌属分类及其主要应用的研究进展.微生物学杂志,2010,30(2):86-90.
[17]Sun DP,Xu J,Zhou LL,et al.Advances on bacterial cellulose fermented by Acetobacter.Journal of Biology,2004,21(1):12-14,22.(in Chinese)孙东平,徐军,周伶俐,等.醋酸杆菌发酵生产细菌纤维素的研究进展.生物学杂志,2004,21(1):12-14,22.
[18]Wang LX,Zhang W,An J.Advances on bacterial cellulose fermented by acetobacter.Journal of Beijing University of Agriculture,2011,26(2):78-80.(in Chinese)王黎霞,张伟,安健.猪密螺旋体痢疾的诊断方法研究进展.北京农学院学报,2011,26(2):78-80.
[19]Chen FL,Wang GC,Teng SO,Ou TY,Yu FL,Lee WS.Clinical and epidemiological features of Chryseobacterium indologenes infections:analysis of 215 cases.Journal of Microbiology,Immunology and Infection,2013,46(6):425-432.
[20]Saleh M,Trinchieri G.Innate immune mechanisms of colitis and colitis-associated colorectal cancer.Nature Reviews Immunology,2011,11(1):9-20.
[2]Wang C,Shi CY,Zhang Y,Song DG,Lu ZQ,Wang YZ.Microbiota in fermented feed and swine gut.Applied Microbiology and Biotechnology,2018,102(7):2941-2948.
[3]Canibe N,Jensen BB.Fermented and nonfermented liquid feed to growing pigs:effect on aspects of gastrointestinal ecology and growth performance.Journal of Animal Science,2003,81(8):2019-2031.
[4]Qin WL.Determination of rumen volatile fatty acids by means of gas chromatography.Journal of Nanjing Agricultural University,1982,5(4):110-116.(in Chinese)秦为琳.应用气相色谱测定瘤胃挥发性脂肪酸方法的研究改进.南京农业大学学报,1982,5(4):110-116.
[5]Khafipour E,Li SC,Plaizier JC,Krause DO.Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis.Applied and Environmental Microbiology,2009,75(22):7115-7124.
[6]Kuroiwa T,Iwanaga M,Kobari K,Higashionna A,Kinjyo F,Saito A.Preventive effect of Clostridium butyricum M588against the proliferation of Clostridium difficile during antimicrobial therapy.The Journal of the Japanese Association for Infectious Diseases,1990,64(11):1425-1432.
[7]Venkatraman A,Ramakrishna BS,Shaji RV,Kumar NSN,Pulimood A,Patra S.Amelioration of dextran sulfate colitis by butyrate:role of heat shock protein 70 and NF-κB.American Journal of Physiology-Gastrointestinal and Liver Physiology,2003,285(1):G177-G184.
[8]Kinoshita M,Suzuki Y,Saito Y.Butyrate reduces colonic paracellular permeability by enhancing PPARγactivation.Biochemical and Biophysical Research Communications,2002,293(2):827-831.
[9]Van Der Meulen R,Makras L,Verbrugghe K,Adriany T,De Vuyst L.In vitro kinetic analysis of oligofructose consumption by Bacteroides and Bifidobacterium spp.indicates different degradation mechanisms.Applied and Environmental Microbiology,2006,72(2):1006-1012.
[10]Ray AK,Roy T,Mondal S,Ring?E.Identification of gut-associated amylase,cellulase and protease‐producing bacteria in three species of Indian major carps.Aquaculture Research,2010,41(10):1462-1469.
[11]Lamendella R,Domingo JWS,Ghosh S,Martinson J,Oerther DB.Comparative fecal metagenomics unveils unique functional capacity of the swine gut.BMC Microbiology,2011,11(1):103-119.
[12]Ohland CL,Mac Naughton WK.Probiotic bacteria and intestinal epithelial barrier function.American Journal of Physiology-Gastrointestinal and Liver Physiology,2010,298(6):G807-G819.
[13]Ashida H,Ogawa M,Kim M,Mimuro H,Sasakawa C.Bacteria and host interactions in the gut epithelial barrier.Nature Chemical Biology,2012,8(1):36-45.
[14]Patterson MF,Mc Kay AM,Connolly M,Linton M.Effect of high pressure on the microbiological quality of cooked chicken during storage at normal and abuse refrigeration temperatures.Food Microbiology,2010,27(2):266-273.
[15]Tingirikari JMR,Kothari D,Goyal A.Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications.Food&Function,2014,5(9):2324-2330.
[16]Feng J,Shi QS,Ouyang YS,et al.Advance in classification and main application of Gluconobacter.Journal of Microbiology,2010,30(2):86-90.(in Chinese)冯静,施庆珊,欧阳友生,等.葡糖杆菌属分类及其主要应用的研究进展.微生物学杂志,2010,30(2):86-90.
[17]Sun DP,Xu J,Zhou LL,et al.Advances on bacterial cellulose fermented by Acetobacter.Journal of Biology,2004,21(1):12-14,22.(in Chinese)孙东平,徐军,周伶俐,等.醋酸杆菌发酵生产细菌纤维素的研究进展.生物学杂志,2004,21(1):12-14,22.
[18]Wang LX,Zhang W,An J.Advances on bacterial cellulose fermented by acetobacter.Journal of Beijing University of Agriculture,2011,26(2):78-80.(in Chinese)王黎霞,张伟,安健.猪密螺旋体痢疾的诊断方法研究进展.北京农学院学报,2011,26(2):78-80.
[19]Chen FL,Wang GC,Teng SO,Ou TY,Yu FL,Lee WS.Clinical and epidemiological features of Chryseobacterium indologenes infections:analysis of 215 cases.Journal of Microbiology,Immunology and Infection,2013,46(6):425-432.
[20]Saleh M,Trinchieri G.Innate immune mechanisms of colitis and colitis-associated colorectal cancer.Nature Reviews Immunology,2011,11(1):9-20.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |