中药诃子抑制金黄色葡萄球菌生物被膜形成机制研究
|
摘要
目的探讨中药诃子对金黄色葡萄球菌(金葡菌)生物被膜形成的抑制作用,为临床联合用药提供理论依据和指导。方法首先检测诃子水提物对游离态金葡菌的抑制效果,之后在建立生物被膜诱导模型基础上评估诃子对生物被膜态金葡菌的抑制效果;进一步评估诃子水提物对金葡菌生物被膜的干预作用,在此基础上探索诃子联合红霉素对金葡菌的抑制效果。结果体外抑菌实验结果显示,诃子水提物对浮游态金葡菌的最小抑菌浓度和最小杀菌浓度分别为3.90mg/ml和31.20mg/ml,对生物被膜态金葡菌的最小抑菌浓度和最小杀菌浓度分别为15.60mg/ml和62.50mg/ml,均具有较好的杀伤效果。生物被膜测定结果显示,诃子水提物对金葡菌生物被膜形成具有抑制作用,呈剂量依赖性。体外联合用药的结果表明,联合使用一定浓度的诃子水提物、红霉素对金葡菌的抑制和杀伤作用显著增强。结论中药诃子对金葡菌生物被膜形成有抑制作用,与红霉素联用能够增强其对金葡菌的杀伤作用,本研究为临床上金葡菌感染的诊治提供了理论指导。
OBJECTIVE To explore the effect of Chinese herb myrobalan on inhibiting the formation of biofilms in Staphylococcus aureus so as to provide guidance for combined use of antibiotics.METHODS Firstly,the effect of herb myrobalan aqueous extracts on S.aureus in suspension was tested,and the inhibitory effect on S.aureus in biofilm state was also examined after the biofilm-induced model was established,the intervention effect of the herb myrobalan aqueous extracts on the S.aureus biofilm was further evaluated,and the inhibitory effect of herb myrobalan combined with erythromycin was explored.RESULTS The result of in vitro bacteriostatic test showed that the minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of herb myrobalan aqueous extracts on S.aureus in suspension were 3.90 mg/ml and 31.20 mg/ml,respectively,the MIC and MBC on S.aureus in biofilm state were 15.60 mg/ml and 62.50 mg/ml,respectively.The result of biofilm test indicated that the herb myrobalan aqueous extracts had inhibitory effect on formation of the S.aureus biofilms in a dose-dependent way.The result of in vitro combined medication showed that erythromycin combined with herb myrobalan aqueous extracts had remarkable inhibitory and lethal effect on the S.aureus.CONCLUSION The herb myrobalan has the inhibitory effect on formation of S.aureus biofilms,and its combination with erythromycin may intensify the lethal effect on the S.aureus strains,which provide theoretical basis for clinical diagnosis and treatment of the S.aureus infection.
OBJECTIVE To explore the effect of Chinese herb myrobalan on inhibiting the formation of biofilms in Staphylococcus aureus so as to provide guidance for combined use of antibiotics.METHODS Firstly,the effect of herb myrobalan aqueous extracts on S.aureus in suspension was tested,and the inhibitory effect on S.aureus in biofilm state was also examined after the biofilm-induced model was established,the intervention effect of the herb myrobalan aqueous extracts on the S.aureus biofilm was further evaluated,and the inhibitory effect of herb myrobalan combined with erythromycin was explored.RESULTS The result of in vitro bacteriostatic test showed that the minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of herb myrobalan aqueous extracts on S.aureus in suspension were 3.90 mg/ml and 31.20 mg/ml,respectively,the MIC and MBC on S.aureus in biofilm state were 15.60 mg/ml and 62.50 mg/ml,respectively.The result of biofilm test indicated that the herb myrobalan aqueous extracts had inhibitory effect on formation of the S.aureus biofilms in a dose-dependent way.The result of in vitro combined medication showed that erythromycin combined with herb myrobalan aqueous extracts had remarkable inhibitory and lethal effect on the S.aureus.CONCLUSION The herb myrobalan has the inhibitory effect on formation of S.aureus biofilms,and its combination with erythromycin may intensify the lethal effect on the S.aureus strains,which provide theoretical basis for clinical diagnosis and treatment of the S.aureus infection.
引文
[1]Gagnaire J,Verhoeven PO,Grattard F,et al.Epidemiology and clinical relevance of Staphylococcus aureus intestinal carriage:a systematic review and meta-analysis[J].Expert Rev Anti Infect Ther,2017,15(8):767-785.
[2]Gaur R,Gupta VK,Singh P,et al.Drug resistance reversal potential of isoliquiritigenin and liquiritigenin isolated from Glycyrrhiza glabra against methicillin-resistant Staphylococcus aureus(MRSA)[J].Phytother Res,2016,30(10):1708-1715.
[3]Chen K,Huang Y,Song Q,et al.Drug-resistance dynamics of Staphylococcus aureus between 2008and 2014at a tertiary teaching hospital,Jiangxi Province,China[J].BMC Infect Dis,2017,17(1):97.
[4]陈朝喜.细菌生物被膜定性和定量研究方法[J].湖北农业科学,2016,55(9):2177-2180.
[5]周至品,杨兴秀,叶晓雪.传统药物对铜绿假单胞菌生物被膜作用研究进展[J].中药药理与临床,2015,31(6):230-235.
[6]李永慧,张志强,吴同磊,等.单味中草药对金黄色葡萄球菌抑菌效果研究[J].健康周刊.2017,16(32):5-7.
[7]张志强,李永慧,吴同垒,等.1株致水貂败血症粘质沙雷菌的分离鉴定[J].中国兽医学报.2018,38(1):148-153.
[8]Clinical and Laboratory Standards Institute.M100S.Performance standards for antimicrobial susceptibility testing:twenty-sixth edition[S].Wayne,PA:CLSI,2016.
[9]李永慧,苏硕青,杨楠,等.大肠杆菌cirA基因缺失对耐药性影响[J].中国人兽共患病学报.2018,34(5):420-423.
[10]乔瑞红,谢鲲鹏,谢明杰,等.和厚朴酚抑制耐甲氧西林金黄色葡萄球菌生物被膜形成[J].微生物学报,2016,56(8):1266-1272.
[11]柳春霞,刘航.蒙药诃子药理作用研究进展[J].北方药学,2015,12(1):91-92.
[12]郑艳阳,曹凤娇,明迪,等.SarA调控金黄色葡萄球菌生物被膜形成的研究进展[J].中国兽药杂志,2017,51(12):62-67.
[13]刘蕾,桂萌,武瑞赟,等.LuxS/AI-2型群体感应系统调控细菌生物被膜形成研究进展[J].食品科学,2016,37(19):254-262.
[14]官妍,陈冉,汪长中,等.苦参碱、鱼腥草素钠及其与红霉素联用对表皮葡萄球菌生物被膜渗透作用的研究[J].中国微生态学杂志.2013,33(2):515-519.
[15]倪玲,肖蕾,陈刚,等.中草药抗白色念珠菌活性成分研究进展[J].中国消毒学杂志,2014,31(5):503-505.
[2]Gaur R,Gupta VK,Singh P,et al.Drug resistance reversal potential of isoliquiritigenin and liquiritigenin isolated from Glycyrrhiza glabra against methicillin-resistant Staphylococcus aureus(MRSA)[J].Phytother Res,2016,30(10):1708-1715.
[3]Chen K,Huang Y,Song Q,et al.Drug-resistance dynamics of Staphylococcus aureus between 2008and 2014at a tertiary teaching hospital,Jiangxi Province,China[J].BMC Infect Dis,2017,17(1):97.
[4]陈朝喜.细菌生物被膜定性和定量研究方法[J].湖北农业科学,2016,55(9):2177-2180.
[5]周至品,杨兴秀,叶晓雪.传统药物对铜绿假单胞菌生物被膜作用研究进展[J].中药药理与临床,2015,31(6):230-235.
[6]李永慧,张志强,吴同磊,等.单味中草药对金黄色葡萄球菌抑菌效果研究[J].健康周刊.2017,16(32):5-7.
[7]张志强,李永慧,吴同垒,等.1株致水貂败血症粘质沙雷菌的分离鉴定[J].中国兽医学报.2018,38(1):148-153.
[8]Clinical and Laboratory Standards Institute.M100S.Performance standards for antimicrobial susceptibility testing:twenty-sixth edition[S].Wayne,PA:CLSI,2016.
[9]李永慧,苏硕青,杨楠,等.大肠杆菌cirA基因缺失对耐药性影响[J].中国人兽共患病学报.2018,34(5):420-423.
[10]乔瑞红,谢鲲鹏,谢明杰,等.和厚朴酚抑制耐甲氧西林金黄色葡萄球菌生物被膜形成[J].微生物学报,2016,56(8):1266-1272.
[11]柳春霞,刘航.蒙药诃子药理作用研究进展[J].北方药学,2015,12(1):91-92.
[12]郑艳阳,曹凤娇,明迪,等.SarA调控金黄色葡萄球菌生物被膜形成的研究进展[J].中国兽药杂志,2017,51(12):62-67.
[13]刘蕾,桂萌,武瑞赟,等.LuxS/AI-2型群体感应系统调控细菌生物被膜形成研究进展[J].食品科学,2016,37(19):254-262.
[14]官妍,陈冉,汪长中,等.苦参碱、鱼腥草素钠及其与红霉素联用对表皮葡萄球菌生物被膜渗透作用的研究[J].中国微生态学杂志.2013,33(2):515-519.
[15]倪玲,肖蕾,陈刚,等.中草药抗白色念珠菌活性成分研究进展[J].中国消毒学杂志,2014,31(5):503-505.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |