怒江州深纹核桃种质资源SSR遗传多样性分析
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摘要
采用SSR分子标记技术对怒江州4个群体122份深纹核桃种质资源进行遗传多样性分析,揭示其多样性水平及遗传结构情况.结果表明:(1)怒江州核桃资源的遗传多样性属于中等水平.4个核桃群体的期望杂合度介于0.503～0.650之间,平均为0.591;Shannon信息指数介于0.937～1.365之间,平均为1.157;Nei′基因多样性指数在0.485～0.641之间,平均为0.576.4个群体中兰坪群体的遗传多样性最高,泸水群体的遗传多样性最低.(2)群体间的遗传分化系数介于0.027 1～0.126 4之间,平均为0.060 9,表明遗传变异主要来自群体内;基因流平均为3.858 3.(3)UPGMA聚类显示4个群体在遗传一致度为0.85时被聚成了4组,兰坪与福贡聚为一组,贡山和泸水分别单独聚为一组,Mantel检验结果表明,群体间遗传距离与地理距离相关性显著(r=0.702 9,P=0.032 9).
To reveal the genetic diversity of walnut germplasm resources in Nujiang Prefecture in Yunnan Province, 122 samples from 4 populations of Juglans sigillata was analyzed by SSR molecular markers technology. The results showed that the genetic diversity of walnut germplasm in Nujiang was at a medium level. The value of expected heterozygosity varied between 0.503 and 0.650, with an average value of 0.591. Shannon′s information index was between 0.937 and 1.365, averaging at 1.157. Nei′s gene diversity ranged from 0.485 to 0.641, with an average value of 0.576. Among 4 populations, Lanping population showed the highest genetic diversity while that of Lushui population was the lowest. The proportion of genetic differentiation among populations ranged between 0.027 1 and 0.126 4, with an average value of 0.060 9, indicating that its genetic variation mainly derived from intragroup resources. The gene flow was 3.858 3. UPGMA cluster analysis showed that populations from Lanping and Fugong clustered as 1 group while Lushui and Gongshan was 2 independent groups. Mantel test indicated that genetic distance was positively related to geographic distance(r=0.702 9, P=0.032 9).
To reveal the genetic diversity of walnut germplasm resources in Nujiang Prefecture in Yunnan Province, 122 samples from 4 populations of Juglans sigillata was analyzed by SSR molecular markers technology. The results showed that the genetic diversity of walnut germplasm in Nujiang was at a medium level. The value of expected heterozygosity varied between 0.503 and 0.650, with an average value of 0.591. Shannon′s information index was between 0.937 and 1.365, averaging at 1.157. Nei′s gene diversity ranged from 0.485 to 0.641, with an average value of 0.576. Among 4 populations, Lanping population showed the highest genetic diversity while that of Lushui population was the lowest. The proportion of genetic differentiation among populations ranged between 0.027 1 and 0.126 4, with an average value of 0.060 9, indicating that its genetic variation mainly derived from intragroup resources. The gene flow was 3.858 3. UPGMA cluster analysis showed that populations from Lanping and Fugong clustered as 1 group while Lushui and Gongshan was 2 independent groups. Mantel test indicated that genetic distance was positively related to geographic distance(r=0.702 9, P=0.032 9).
引文
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[23] 廖柏勇,王芳,陈丽君,等.基于SRAP分子标记的苦楝种质资源遗传多样性分析[J].林业科学,2016,52(4):48-58.
[24] 文亚峰,韩文军,吴顺.植物遗传多样性及其影响因素[J].中南林业科技大学学报,2010,30(12):80-87.
[25] SLATKIN M, BARTON N H. A comparison of three indirect methods for estimating average levels of gene flow[J]. Evolution, 1989,43(7):1 349-1 368.
[26] HAMRICK J L, GODT M J W, SHERMAN-BROYLES L S. Gene flow among plant populations: Evidence from genetic markers[C]//HOCH D C, STEPHNON A G. Experimental and Molecular Approaches to Plant Biosystematics. Missouri Botanical Garden, 1995:215-232.
[27] 陈良华,胡庭兴,张帆,等.用AFLP技术分析四川核桃资源的遗传多样性[J].植物生态学报,2008,32(6):1 362-1 372.
[28] 顾万春.统计遗传学[M].北京:科学出版社,2006:98-101.
[29] 张登福,任列花.13个雄先型早实核桃品种孤雌生殖和自交及自然授粉结实试验[J].林业实用技术,2013(12):30-31.
[30] KRUTOVSKY K V, CLAIR J B S, SAICH R, et al. Estimate of population structure in coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] using allozyme and microsatellite markers[J]. Tree Genetic & Genomes, 2009,5(4):641-658.
[2] 莫傲,王生.怒江州生态安全屏障构建思路[J].林业调查规划,2017(1):54-58.
[3] AGARWAL M, SHRIVASTAVA N, PADH H. Advances in molecular marker techniques and their applications in plant sciences[J]. Plant Cell Reports, 2008,27(4):617-631.
[4] 徐永杰,韩华柏,王滑,等.大巴山区核桃实生居群的坚果表型和遗传多样性[J].林业科学,2016,52(5):111-118.
[5] 金强,杨宇,王新建,等.新疆核桃种质资源遗传多样性AFLP分析[J].江苏农业科学,2009(2):28-31.
[6] 郝艳宾,黄武刚,王克建,等.中国核桃组(Sect.Juglans)种质资源的SSR标记分析[J].果树学报,2007,24(5):620-625.
[7] 王滑,郝俊民,王宝庆,等.中国核桃8个天然居群遗传多样性分析[J].林业科学,2007,43(7):120-124.
[8] WANG H, PEI D, GU R S, et al. Genetic diversity and structure of walnut populations in central and southwestern China revealed by microsatellite markers[J]. Journal America Soc Horticulture Science, 2008,133(2):197-203.
[9] BAYAZIT S, KAZAN K. AFLP analysis of genetic diversity in low chill requiring walnut (Juglans regia L.) genotypes from Hatay, Turkey[J]. Scientia Horticulturae, 2007,111(4):394-398.
[10] CHEN L N, MA Q G, CHEN Y K, et al. Identification of major walnut cultivars grown in China based on nut phenotypes and SSR markers[J]. Scientia Horticulturae, 2014,168:240-248.
[11] 杨恩,陈少瑜,张雨,等.漾濞核桃叶片基因组DNA的两种提取方法效果比较[J].西部林业科学,2005,34(4):72-75.
[12] 陈少瑜,宁德鲁,吴涛,等.泡核桃SSR标记开发及在遗传多样性研究中的应用[J].西北林学院学报,2017,32(3):91-96.
[13] YEH F C, YANG R C, BOYLE T B J, et al. POPGENE, The User-Friendly Shareware For Population Genetic Analysis[M]. Molecular Biology and Biotechnology Center, Canada: University of Alberta, 1997.
[14] HARTL D L, CLARK A G. Principles of Population Genetics[M]. 2nd ed. Sunderland, Massachusetts: Sinauer Associates, Inc., 1989.
[15] SLATKIN M, BARTON N H. A comparison of three indirect methods for estimating average levels of gene flow[J]. Evolution, 1989,43(7):1 349-1 368.
[16] TAMURA K, STECHER G, PETERSON D, et al. MEGA6: Molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution, 2013,30(12):2 725-2 729.
[17] MANTEL N. The detection of disease clustering and a generalized regression approach[J]. Cancer Research, 1967,27(2):209-220.
[18] 黄勇.基于SRAP分子标记的小果油茶遗传多样性分析[J].林业科学,2013,49(3):43-50.
[19] 徐刚标.植物群体遗传学[M].北京:科学出版社,2011.
[20] 刘晓丽,陈学森,张美勇,等.普通核桃(Juglans regia)3个群体遗传结构的SSR分析[J].果树学报,2008,25(4):526-530.
[21] WANG H, PAN G, MA Q G, et al. The genetic diversity and introgression of Juglans regia and Juglans sigillata in Tibet as revealed by SSR markers[J]. Tree Genetics & Genomes, 2015,11(1):1-11.
[22] VICTORY E R, GLAUBITZ J C, RHODES O E, et al. Genetic homogeneity in Juglans nigra (Juglandaceae) at nuclear microsatellites[J]. Am J Bot, 2006,93(1):118-126.
[23] 廖柏勇,王芳,陈丽君,等.基于SRAP分子标记的苦楝种质资源遗传多样性分析[J].林业科学,2016,52(4):48-58.
[24] 文亚峰,韩文军,吴顺.植物遗传多样性及其影响因素[J].中南林业科技大学学报,2010,30(12):80-87.
[25] SLATKIN M, BARTON N H. A comparison of three indirect methods for estimating average levels of gene flow[J]. Evolution, 1989,43(7):1 349-1 368.
[26] HAMRICK J L, GODT M J W, SHERMAN-BROYLES L S. Gene flow among plant populations: Evidence from genetic markers[C]//HOCH D C, STEPHNON A G. Experimental and Molecular Approaches to Plant Biosystematics. Missouri Botanical Garden, 1995:215-232.
[27] 陈良华,胡庭兴,张帆,等.用AFLP技术分析四川核桃资源的遗传多样性[J].植物生态学报,2008,32(6):1 362-1 372.
[28] 顾万春.统计遗传学[M].北京:科学出版社,2006:98-101.
[29] 张登福,任列花.13个雄先型早实核桃品种孤雌生殖和自交及自然授粉结实试验[J].林业实用技术,2013(12):30-31.
[30] KRUTOVSKY K V, CLAIR J B S, SAICH R, et al. Estimate of population structure in coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] using allozyme and microsatellite markers[J]. Tree Genetic & Genomes, 2009,5(4):641-658.
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