生物法降解黄曲霉毒素B_1的机理研究进展
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摘要
黄曲霉毒素B_1是由寄生曲霉、黄曲霉等真菌产生的次级代谢物,具有极强的致畸、致癌等毒性,不仅对人和动物健康构成严重的威胁,而且也给食品和饲料工业带来巨大的经济损失。如何消除黄曲霉毒素B_1的污染依然是目前学者迫切要解决的重点问题。相比理化法消除黄曲霉毒素B_1的缺点和局限,生物法降解具有安全、高效和绿色环保等优点,使其成为更具有潜力的解毒方法。本文主要对生物吸附作用和生物酶解作用降解黄曲霉毒素B_1的机理及降解产物的结构分析进行了论述。
Aflatoxin B_1(AFB_1),with strong toxicity and lead to macrocephaly,cancer and mutation,is a group of secondary metabolites produced by the Aspergillus flavus,Aspergillus parasitieus and some other fungies,which not only poses a serious threat to animals and humans,but also cause tremendous economies loss.Nowadays,AFB_1 pollution is regarded as a common phenomenon in the food and feed industry,yet there is still some difficulty on the methods to eliminate it.Compared with physicochemical method,the biologic degradation has the advantages of effective,safe and environment-friendly.This article introduces the biological detoxication especially the adsorption and degradation of AFB_1 by microorganism.
Aflatoxin B_1(AFB_1),with strong toxicity and lead to macrocephaly,cancer and mutation,is a group of secondary metabolites produced by the Aspergillus flavus,Aspergillus parasitieus and some other fungies,which not only poses a serious threat to animals and humans,but also cause tremendous economies loss.Nowadays,AFB_1 pollution is regarded as a common phenomenon in the food and feed industry,yet there is still some difficulty on the methods to eliminate it.Compared with physicochemical method,the biologic degradation has the advantages of effective,safe and environment-friendly.This article introduces the biological detoxication especially the adsorption and degradation of AFB_1 by microorganism.
引文
[1]陈仪本,蔡斯赞,黄伯爱,等.生物学法降解花生油中黄曲霉毒素的研究[J].卫生研究,1998,27(S1):79~83.
[2]程伟,左瑞雨,常娟,等.益生菌和黄曲霉毒素B1降解酶对肉仔鸡生长性能的影响及其作用机理[J].动物营养学报,2014,26(6):1608~1615.
[3]戴军.黄曲霉毒素B1降解酶产生菌的筛选及发酵制备酶制剂的研究:[硕士学位论文][D].武汉:湖北工业大学,2015.
[4]胡常英,胡科峰,秦慧娟,等.复合酶制剂降解黄曲霉毒素B1对蛋鸡生产性能和蛋品质的影响[J].生物学杂志,2015,2:24~27.
[5]姜富贵,韩红,陈雪梅,等.乳酸菌和纳豆芽孢杆菌对黄曲霉毒素B1和伏马毒素B1的吸附作用研究[J].山东农业科学,2018,50(1):127~130.
[6]刘畅,刘阳,邢福国,等.黄曲霉毒素B1吸附菌株的筛选及吸附机理研究[J].核农学报,2010,24(4):766~771.
[7]刘睿杰,孙丰芹,常明,等.巨大芽孢杆菌固态发酵法去除黄曲霉毒素B1工艺对花生粕品质的影响[J].中国油脂,2012,37(12):18~21.
[8]钱潘攀,李红波,赵岩岩,等.双效型酵母细胞壁提取物及其对黄曲霉毒素B1吸附特性研究[J].食品工业科技,2017,38(15):25~29.
[9]沈祥震.花生油中黄曲霉毒素B1紫外降解及安全性评价[硕士学位论文][D].泰安:山东农业大学,2014.
[10]王雪妍,陈晓飞,周伏忠,等.一株具有黄曲霉毒素B1降解功能的食醚红球菌的分离鉴定[J].河南科学,2017,35(7):1070~1074.
[11]王勇,赵秀平,王韧,等.微波辅助碱法降解黄曲霉毒素工艺对米蛋白品质的影响[J].中国粮油学报,2014,29(4):1~4.
[12]杨乃欢,李彪.新型霉菌毒素吸附剂-酵母细胞壁提取物[J].中国畜牧业,2009,21:23~24.
[13]杨玉红.乳酸菌对真菌毒素的吸附效果研究[J].粮油加工,2009,6:140~143.
[14]翟翠萍.红平红球菌的培养及其对黄曲霉毒素B1的降解研究[硕士学位论文][D].青岛:中国海洋大学,2012.
[15]张晓雪.诱变黑曲霉及其固定化对黄曲霉毒素B1的脱毒作用及安全性评价:[硕士学位论文][D].无锡:江南大学,2015.
[16]张秀江,胡虹,张永战,等.酵母菌对饲料中黄曲霉毒素B1的降解和脱除作用研究[J].河南科学,2016,34(10):1686~1690.
[17]赵春霞,王轶,程薇,等.复合菌系降解黄曲霉毒素B1的效果及组成多样性研究[J].食品科学,2017,38(9):106~112.
[18]赵丽红,高欣,马秋刚,等.黄曲霉毒素降解菌对饲喂含AFB1霉变玉米日粮蛋鸡生产性能和蛋品质的影响[J].中国畜牧杂志,2012,48(11):31~35.
[19]周建新,吴萌萌,包月红,等.臭氧处理稻谷降解黄曲霉毒素B1的工艺条件优化[J].粮食储藏,2014,43(4):17~21.
[20]周露,王会娟,邢福国,等.平菇P1培养条件优化及其黄曲霉毒素降解酶的初步分离[J].核农学报,2014,28(9):1625~1631.
[21]Abrar M,Anjum F M,Butt M S,et al.Aflatoxins:Biosynthesis,Occurrence,Toxicity,and Remedies[J].Critical Reviews in Food Science and Nutrition,2013,53(8):862~874.
[22]Adebo O A,Njobeh P B,Sibusiso S,et al.Aflatoxin B1 degradation by liquid cultures and lysates of three bacterial strains[J].International Journal of Food Microbiology,2016,233:11~19.
[23]Alberts J F,Engelbrecht Y,Steyn P S,et al.Biological degradation of aflatoxin B1by Rhodococcus erythropolis cultures[J].International Journal of Food Microbiology,2006,109(1~2):121~126.
[24]Chen Y,Kong Q,Chi C,et al.Biotransformation of aflatoxin B1and aflatoxin G1in peanut meal by anaerobic solid fermentation of Streptococcus thermophilus and Lactobacillus delbrueckii subsp.bulgaricus[J].International Journal of Food Microbiology,2015,211:1~5.
[25]Ciegler A,Lillehoj B,Peterson R E,et al.Mierobial.Detoxieation of Aflatoxins[J].Appl Microbiol,1966,14:934~939.
[26]Das A,Bhattacharya S,Palaniswamy M,et al.Biodegradation of aflatoxin B1in contaminated rice straw by Pleurotus ostreatus MTCC 142 and Pleurotus ostreatus GHBBF10 in the presence of metal salts and surfactants[J].World Journal of Microbiology and Biotechnology,2014,30(8):2315~2324.
[27]Detroy R,Hesseltine C.Transformation of aflatoxin B1by steroid-hydroxylating fungi[J].Canadian Journal of Microbiology,1969,15(6):495~500.
[28]Doyle M P,Marth E H.Peroxidase activity in mycelia of Aspergillus parasiticus,that degrade aflatoxin[J].European Journal of Applied Microbiology and Biotechnology,1979,7(2):211~217.
[29]Gao X,Ma Q,Zhao L,et al.Isolation of Bacillus subtilis:screening for aflatoxins B1,M1,and G1detoxification[J].European Food Research and Technology,2011,232(6):957~962.
[30]Guan S,Ji C,Zhou T,et al.Aflatoxin B1Degradation by Stenotrophomonas Maltophilia and Other Microbes Selected Using Coumarin Medium[J].International Journal of Molecular Sciences,2008,9(8):1489~1503.
[31]Hamidi A,Mirnejad R,Yahaghi E,et al.The aflatoxin B1isolating potential of two lactic acid bacteria[J].Asian Pacific Journal of Tropical Biomedicine,2013,3(9):732~736.
[32]Hernandez-Mendoza A,Garcia H S,Steele J L.Screening of Lactobacillus casei strains for their ability to bind aflatoxin B1[J].Food and Chemical Toxicology,2009,47(6):1064~1068.
[33]Hernandez-Mendoza A,Guzman-de-Pena D,Garcia H S.Key role of teichoic acids on aflatoxin B1binding by probiotic bacteria[J].Journal of Applied Microbiology,2009,107(2):395~403.
[34]Hormisch D,Brost I,Kohring G W,et al.Mycobacterium fluoranthenivorans sp.nov.A fluoranthene and aflatoxin B1degrading bacterium from contaminated soil of a former coal gas plant[J].Systematic and Applied Microbiology,2004,27(6):653~660.
[35]Lee Y K,El-Nezami H,Haskard C A,et al.Kinetics of adsorption and desorption of aflatoxin B1by viable and nonviable bacteria[J].Journal of Food Protection,2003,66(3):426~430.
[36]Liu D L,Yao D S,Liang I R,et al.Detoxification of aflatoxin B1by enzymes isolated from Armillariella tabescens[J].Food and Chemical Toxicology,1998,36(7):563~574.
[37]Moretti A F,Gamba R R,Puppo J,et al.Incorporation of Lactobacillus plantarum and zeolites in poultry feed can reduce aflatoxin B1levels[J].Journal of Food Science and Technology,2018,55(1):431~436.
[38]Motomura M,Toyomasu T,Mizuno K,et al.Purification and characterization of an aflatoxin degradation enzyme from Pleurotus ostreatus[J].Microbiological Research,2003,158(3):237~242.
[39]Oluwafemi F,Kumar M,Bandyopadhyay R,et al.Bio-detoxification of aflatoxin B1in artificially contaminated maize grains using lactic acid bacteria[J].Toxin Reviews,2010,29(3~4):115~122.
[40]Oruc H H,Cibik R,Yilmaz E,et al.Distribution and stability of Aflatoxin M1during processing and ripening of traditional white pickled cheese[J].Food Additives and Contaminants,2006,23(2):190~195.
[41]Samuel M S,Sivaramakrishna A,Mehta A.Degradation and detoxification of aflatoxin B1by Pseudomonas putida[J].International Biodeterioration and Biodegradation,2014,86(1):202~209.
[42]Shantha T.Fungal degradation of aflatoxin B1[J].Natural Toxins,1999,7(5):175~178.
[43]Teniola O D,Addo P A,Brost I M,et al.Degradation of aflatoxin B1by cell-free extracts of Rhodococcus erythropolis and Mycobacterium fluoranthenivorans sp.nov.DSM44556T[J].International Journal of Food Microbiology,2005,105(2):111~117.
[44]Wang G,Lian C,Xi Y,et al.Evaluation of nonionic surfactant modified montmorillonite as mycotoxins adsorbent for aflatoxin B1and zearalenone[J].Journal of Colloid and Interface Science,2018,518:48~56.
[45]Wang J,Ogata M,Hirai H,et al.Detoxification of aflatoxin B1by manganese peroxidase from the white-rot fungus Phanerochaete sordida YK-624[J].FEMS Microbiology Letters,2011,314(2):164~169.
[46]Wang Y,Zhao C,Zhang D,et al.Effective degradation of aflatoxin B1 using a novel thermophilic microbial consortium TADC7[J].Bioresource Technology,2017,224:166~173.
[47]Yazdanpanah H,Mohammadi T,Abouhossain G,et al.Effect of roasting on degradation of Aflatoxins in contaminated pistachio nuts[J].Food and Chemical Toxicology,2005,43(7):1135~1139.
[48]Zhang W,Xue B,Li M,et al.Screening a Strain of Aspergillus niger and Optimization of Fermentation Conditions for Degradation of Aflatoxin B1[J].Toxins,2014,6(11):3157~3172.
[49]Zhao L H,Guan S,Gao X,et al.Preparation,purification and characteristics of an aflatoxin degradation enzyme from Myxococcus fulvus ANSM068[J].Journal of Applied Microbiology,2011,110(1):147~145.
[2]程伟,左瑞雨,常娟,等.益生菌和黄曲霉毒素B1降解酶对肉仔鸡生长性能的影响及其作用机理[J].动物营养学报,2014,26(6):1608~1615.
[3]戴军.黄曲霉毒素B1降解酶产生菌的筛选及发酵制备酶制剂的研究:[硕士学位论文][D].武汉:湖北工业大学,2015.
[4]胡常英,胡科峰,秦慧娟,等.复合酶制剂降解黄曲霉毒素B1对蛋鸡生产性能和蛋品质的影响[J].生物学杂志,2015,2:24~27.
[5]姜富贵,韩红,陈雪梅,等.乳酸菌和纳豆芽孢杆菌对黄曲霉毒素B1和伏马毒素B1的吸附作用研究[J].山东农业科学,2018,50(1):127~130.
[6]刘畅,刘阳,邢福国,等.黄曲霉毒素B1吸附菌株的筛选及吸附机理研究[J].核农学报,2010,24(4):766~771.
[7]刘睿杰,孙丰芹,常明,等.巨大芽孢杆菌固态发酵法去除黄曲霉毒素B1工艺对花生粕品质的影响[J].中国油脂,2012,37(12):18~21.
[8]钱潘攀,李红波,赵岩岩,等.双效型酵母细胞壁提取物及其对黄曲霉毒素B1吸附特性研究[J].食品工业科技,2017,38(15):25~29.
[9]沈祥震.花生油中黄曲霉毒素B1紫外降解及安全性评价[硕士学位论文][D].泰安:山东农业大学,2014.
[10]王雪妍,陈晓飞,周伏忠,等.一株具有黄曲霉毒素B1降解功能的食醚红球菌的分离鉴定[J].河南科学,2017,35(7):1070~1074.
[11]王勇,赵秀平,王韧,等.微波辅助碱法降解黄曲霉毒素工艺对米蛋白品质的影响[J].中国粮油学报,2014,29(4):1~4.
[12]杨乃欢,李彪.新型霉菌毒素吸附剂-酵母细胞壁提取物[J].中国畜牧业,2009,21:23~24.
[13]杨玉红.乳酸菌对真菌毒素的吸附效果研究[J].粮油加工,2009,6:140~143.
[14]翟翠萍.红平红球菌的培养及其对黄曲霉毒素B1的降解研究[硕士学位论文][D].青岛:中国海洋大学,2012.
[15]张晓雪.诱变黑曲霉及其固定化对黄曲霉毒素B1的脱毒作用及安全性评价:[硕士学位论文][D].无锡:江南大学,2015.
[16]张秀江,胡虹,张永战,等.酵母菌对饲料中黄曲霉毒素B1的降解和脱除作用研究[J].河南科学,2016,34(10):1686~1690.
[17]赵春霞,王轶,程薇,等.复合菌系降解黄曲霉毒素B1的效果及组成多样性研究[J].食品科学,2017,38(9):106~112.
[18]赵丽红,高欣,马秋刚,等.黄曲霉毒素降解菌对饲喂含AFB1霉变玉米日粮蛋鸡生产性能和蛋品质的影响[J].中国畜牧杂志,2012,48(11):31~35.
[19]周建新,吴萌萌,包月红,等.臭氧处理稻谷降解黄曲霉毒素B1的工艺条件优化[J].粮食储藏,2014,43(4):17~21.
[20]周露,王会娟,邢福国,等.平菇P1培养条件优化及其黄曲霉毒素降解酶的初步分离[J].核农学报,2014,28(9):1625~1631.
[21]Abrar M,Anjum F M,Butt M S,et al.Aflatoxins:Biosynthesis,Occurrence,Toxicity,and Remedies[J].Critical Reviews in Food Science and Nutrition,2013,53(8):862~874.
[22]Adebo O A,Njobeh P B,Sibusiso S,et al.Aflatoxin B1 degradation by liquid cultures and lysates of three bacterial strains[J].International Journal of Food Microbiology,2016,233:11~19.
[23]Alberts J F,Engelbrecht Y,Steyn P S,et al.Biological degradation of aflatoxin B1by Rhodococcus erythropolis cultures[J].International Journal of Food Microbiology,2006,109(1~2):121~126.
[24]Chen Y,Kong Q,Chi C,et al.Biotransformation of aflatoxin B1and aflatoxin G1in peanut meal by anaerobic solid fermentation of Streptococcus thermophilus and Lactobacillus delbrueckii subsp.bulgaricus[J].International Journal of Food Microbiology,2015,211:1~5.
[25]Ciegler A,Lillehoj B,Peterson R E,et al.Mierobial.Detoxieation of Aflatoxins[J].Appl Microbiol,1966,14:934~939.
[26]Das A,Bhattacharya S,Palaniswamy M,et al.Biodegradation of aflatoxin B1in contaminated rice straw by Pleurotus ostreatus MTCC 142 and Pleurotus ostreatus GHBBF10 in the presence of metal salts and surfactants[J].World Journal of Microbiology and Biotechnology,2014,30(8):2315~2324.
[27]Detroy R,Hesseltine C.Transformation of aflatoxin B1by steroid-hydroxylating fungi[J].Canadian Journal of Microbiology,1969,15(6):495~500.
[28]Doyle M P,Marth E H.Peroxidase activity in mycelia of Aspergillus parasiticus,that degrade aflatoxin[J].European Journal of Applied Microbiology and Biotechnology,1979,7(2):211~217.
[29]Gao X,Ma Q,Zhao L,et al.Isolation of Bacillus subtilis:screening for aflatoxins B1,M1,and G1detoxification[J].European Food Research and Technology,2011,232(6):957~962.
[30]Guan S,Ji C,Zhou T,et al.Aflatoxin B1Degradation by Stenotrophomonas Maltophilia and Other Microbes Selected Using Coumarin Medium[J].International Journal of Molecular Sciences,2008,9(8):1489~1503.
[31]Hamidi A,Mirnejad R,Yahaghi E,et al.The aflatoxin B1isolating potential of two lactic acid bacteria[J].Asian Pacific Journal of Tropical Biomedicine,2013,3(9):732~736.
[32]Hernandez-Mendoza A,Garcia H S,Steele J L.Screening of Lactobacillus casei strains for their ability to bind aflatoxin B1[J].Food and Chemical Toxicology,2009,47(6):1064~1068.
[33]Hernandez-Mendoza A,Guzman-de-Pena D,Garcia H S.Key role of teichoic acids on aflatoxin B1binding by probiotic bacteria[J].Journal of Applied Microbiology,2009,107(2):395~403.
[34]Hormisch D,Brost I,Kohring G W,et al.Mycobacterium fluoranthenivorans sp.nov.A fluoranthene and aflatoxin B1degrading bacterium from contaminated soil of a former coal gas plant[J].Systematic and Applied Microbiology,2004,27(6):653~660.
[35]Lee Y K,El-Nezami H,Haskard C A,et al.Kinetics of adsorption and desorption of aflatoxin B1by viable and nonviable bacteria[J].Journal of Food Protection,2003,66(3):426~430.
[36]Liu D L,Yao D S,Liang I R,et al.Detoxification of aflatoxin B1by enzymes isolated from Armillariella tabescens[J].Food and Chemical Toxicology,1998,36(7):563~574.
[37]Moretti A F,Gamba R R,Puppo J,et al.Incorporation of Lactobacillus plantarum and zeolites in poultry feed can reduce aflatoxin B1levels[J].Journal of Food Science and Technology,2018,55(1):431~436.
[38]Motomura M,Toyomasu T,Mizuno K,et al.Purification and characterization of an aflatoxin degradation enzyme from Pleurotus ostreatus[J].Microbiological Research,2003,158(3):237~242.
[39]Oluwafemi F,Kumar M,Bandyopadhyay R,et al.Bio-detoxification of aflatoxin B1in artificially contaminated maize grains using lactic acid bacteria[J].Toxin Reviews,2010,29(3~4):115~122.
[40]Oruc H H,Cibik R,Yilmaz E,et al.Distribution and stability of Aflatoxin M1during processing and ripening of traditional white pickled cheese[J].Food Additives and Contaminants,2006,23(2):190~195.
[41]Samuel M S,Sivaramakrishna A,Mehta A.Degradation and detoxification of aflatoxin B1by Pseudomonas putida[J].International Biodeterioration and Biodegradation,2014,86(1):202~209.
[42]Shantha T.Fungal degradation of aflatoxin B1[J].Natural Toxins,1999,7(5):175~178.
[43]Teniola O D,Addo P A,Brost I M,et al.Degradation of aflatoxin B1by cell-free extracts of Rhodococcus erythropolis and Mycobacterium fluoranthenivorans sp.nov.DSM44556T[J].International Journal of Food Microbiology,2005,105(2):111~117.
[44]Wang G,Lian C,Xi Y,et al.Evaluation of nonionic surfactant modified montmorillonite as mycotoxins adsorbent for aflatoxin B1and zearalenone[J].Journal of Colloid and Interface Science,2018,518:48~56.
[45]Wang J,Ogata M,Hirai H,et al.Detoxification of aflatoxin B1by manganese peroxidase from the white-rot fungus Phanerochaete sordida YK-624[J].FEMS Microbiology Letters,2011,314(2):164~169.
[46]Wang Y,Zhao C,Zhang D,et al.Effective degradation of aflatoxin B1 using a novel thermophilic microbial consortium TADC7[J].Bioresource Technology,2017,224:166~173.
[47]Yazdanpanah H,Mohammadi T,Abouhossain G,et al.Effect of roasting on degradation of Aflatoxins in contaminated pistachio nuts[J].Food and Chemical Toxicology,2005,43(7):1135~1139.
[48]Zhang W,Xue B,Li M,et al.Screening a Strain of Aspergillus niger and Optimization of Fermentation Conditions for Degradation of Aflatoxin B1[J].Toxins,2014,6(11):3157~3172.
[49]Zhao L H,Guan S,Gao X,et al.Preparation,purification and characteristics of an aflatoxin degradation enzyme from Myxococcus fulvus ANSM068[J].Journal of Applied Microbiology,2011,110(1):147~145.