丹参、黄芪注射液对自体脂肪移植存活率的影响
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摘要
目的观察中药丹参、黄芪注射液对自体脂肪移植成活率的影响,为后续实验及临床应用提供依据。方法建立新西兰白兔自体脂肪移植动物模型,实验组分别予以腹腔注射丹参、黄芪注射液,于不同时间点采血、取材,测定血清VEGF表达水平和脂肪存活率,HE染色观察组织细胞形态,血小板-内皮细胞黏附分子CD31、周脂素免疫组化染色观察毛细血管密度及细胞活性。结果自体脂肪移植术后第12周,实验组脂肪存活率显著高于对照组;各时间点实验组血清VEGF表达水平均显著高于对照组;HE、CD31、周脂素染色示,实验组在细胞形态、新生毛细血管密度及细胞活性方面均明显优于对照组。结论配合应用丹参、黄芪注射液,能够显著提高新西兰白兔自体脂肪移植的存活率。
Objective To explore the effects of Salvia miltiorrhiza and Astragalus propinquus injection on the survival of transplanted grafts,so as to provide evidence for further research and clinical application.Methods Autologous adipose tissue transplantation model was established using New Zealand white rabbit.The experimental groups were given S.miltiorrhiza and A.propinquus injection intraperitoneally.The expression levels of VEGF in serum and survival rates were measured and calculated at different time points.Tissue morphology was observed by HE staining.The expression of platelet endothelial cell adhesion molecule-1(CD31) and perilipin were observed by immune-histochemistry to evaluate the neocapillary growth and cyto-activity of transplanted adipose tissue.Results Twelve weeks after transplantation,the survival rates in experimental groups were higher than the control group.The expression levels of VEGF in serum in experimental groups were significantly higher than those in the control group at each time point.HE and immuno-histochemical staining demonstrated better morphology,denser neo-capillaries and higher cyto-activity in experimental groups,compared with the control group.Conclusion By applying S.miltiorrhiza and A.propinquus injection,the survival of autologous transplanted adipose tissue can be significantly enhanced in New Zealand rabbits.
Objective To explore the effects of Salvia miltiorrhiza and Astragalus propinquus injection on the survival of transplanted grafts,so as to provide evidence for further research and clinical application.Methods Autologous adipose tissue transplantation model was established using New Zealand white rabbit.The experimental groups were given S.miltiorrhiza and A.propinquus injection intraperitoneally.The expression levels of VEGF in serum and survival rates were measured and calculated at different time points.Tissue morphology was observed by HE staining.The expression of platelet endothelial cell adhesion molecule-1(CD31) and perilipin were observed by immune-histochemistry to evaluate the neocapillary growth and cyto-activity of transplanted adipose tissue.Results Twelve weeks after transplantation,the survival rates in experimental groups were higher than the control group.The expression levels of VEGF in serum in experimental groups were significantly higher than those in the control group at each time point.HE and immuno-histochemical staining demonstrated better morphology,denser neo-capillaries and higher cyto-activity in experimental groups,compared with the control group.Conclusion By applying S.miltiorrhiza and A.propinquus injection,the survival of autologous transplanted adipose tissue can be significantly enhanced in New Zealand rabbits.
引文
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[19]Yoshimura K,Shigeura T,Matsumoto D,et al.Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates[J].J Cell Physiol,2006,208(1):64-76.
[20]毋巨龙,李世荣.bFGF对颗粒脂肪移植成活率的影响及CT影像学研究[J].重庆医学,2008,37(12):1314-1315.
[21]Sato K,Nakanishi N,Mitsumoto M.Culture condition supporting adipocyte conversion of stromal-vascular cells from bovine intramuscullar adipocyte tissues[J].J Vet Med Sci,1996,58(2):1073-1078.
[22]梁杰,银桂彬,彭智,等.血管生成素协同血管内皮细胞生长因子对自体脂肪移植血运重建的影响[J].中国美容医学,2007,16(7):891-894.
[23]彭青和.瘦素影响SD大鼠移植颗粒脂肪成活率的实验研究[D].桂林:桂林医学院.2010.
[24]李锋,李昆,李杰,等.富血小板血浆对颗粒脂肪移植的影响[J].中国组织工程研究,2013,17(31):5645-5651.
[25]Craft RO,Rophael J,Morrison WA,et al.Effect of local,longterm delivery of platelet-derived growth factor(PDGF)on injected fat graft survival in severe combined immunodeficient(SCID)mice[J].J Plast Reconstr Aesthet Surg,2009,62(2):235-243.
[26]梁杰,赵坤,吴浩俊,等.重组人VEGF和前列腺素E1对移植脂肪早期血管生成的作用[J].中国实用医药,2009,4(11):7-9.
[27]Plate KH,Breier G,Weich HA,et al.Vascular endothelial growth factor is a potential tumour angiogenesis factor in human gliomas in vivo[J].Nature,1992,359(6398):845-848.
[28]付小兵,程飚,盛志勇.生长因子应用于临床创伤修复——十年的主要进展与展望[J].中国修复重建外科杂志,2004,18(6):508-512.
[29]黄双盛,吴肋杰.丹参的抗氧化与抗炎作用研究进展[J].中国中医药信息杂志,2002,9(1):86-87.
[30]李莉,刘耕陶.丹酚酸A对大鼠脑突触体和线粒体氧应激损伤的体外保护作用[J].中国药理学与毒理学杂志,1998,12(4):275-279.
[31]武承兴,王健,张波,等.黄芪对自体脂肪颗粒移植存活率的影响[J].组织工程与重建外科,2013,9(3):141-144.
[2]Rietjens M,De Lorenzi F,Rossetto F,et al.Safety of fat grafting in secondary breast reconstruction after cancer[J].J Plast Reconstr Aesthet Surg,2011,64(3):477-483.
[3]张涤生.张涤生整复外科学[M].上海:上海科学技术出版社,2002.
[4]Nishimura T,Hashimoto H,Nakanishi I,et al.Microvascular angiogenesis and apoptosis in the survival of free fat grafts[J].Laryngoscope,2000,110(8):1333-1338.
[5]Ellenbogen R.Free autogenous pearl fat grafts in the face-a preliminary report of a rediscovered technique[J].Ann Plast Surg,1986,16(3):179-194.
[6]Yi CG,Xia W,Zhang LX,et al.VEGF gene therapy for the survival of transplanted fat tissue in nude mice[J].J Plast Reconstr Aesthet Surg,2007,60(3):272-278.
[7]马丙祥,董宠凯.丹参的药理作用研究新进展[J].中国药房,2014,25(7):663-665.
[8]宋伟.丹参的药理作用的研究进展[J].北方药学,2013,10(5):52-53.
[9]杨雪鸱,王小云,开国银,等.丹参酮ⅡA抗肿瘤作用及其新剂型的研究进展[J].中成药,2011,33(8):1389-1392.
[10]张晓雷,陈俊华,郭春霞,等.丹参多酚酸盐的药理作用研究[J].世界临床药物.2013.34(5):292-297.
[11]肖志华.丹参的临床应用[J].中外医学研究2012,10(36):154-155.
[12]朱瑄.黄芪的现代药理研究及其临床新用[J].中国中医药现代远程教育.2010.8(15):72-72.
[13]袁红霞,陈艳春.黄芪的现代药理研究及其临床应用[J].山东中医药大学学报.2000.24(5):397-400.
[14]陈国辉,黄文凤.黄芪的化学成分及药理作用研究进展[J].中国新药杂志,2008.17(17):1482-1485.
[15]葛斌,许爱霞,杨社华.黄芪多糖抗衰老作用机制的研究[J].中国医院药学杂志.2004.24(10):610-612.
[16]Zhao J,Yi C,Zheng Y,et al.Enhancement of fat graft survival by bone marrow-derived mesenchymal stem cell therapy[J].Plast Reconstr Surg,2013,132(5):l 149-1157.
[17]吴慧玲.自体颗粒脂肪移植成活率的实验研究及川芎嗪对提高脂肪移植成活率的影响[D].杭州:浙江大学,2009.
[18]Hamed S,Egozi D,Kruchevsky D,et al.Erythropoietin improves the survival of fat tissue after its transplantation in nude mice[J].Plos One,2010,5(11):419-453.
[19]Yoshimura K,Shigeura T,Matsumoto D,et al.Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates[J].J Cell Physiol,2006,208(1):64-76.
[20]毋巨龙,李世荣.bFGF对颗粒脂肪移植成活率的影响及CT影像学研究[J].重庆医学,2008,37(12):1314-1315.
[21]Sato K,Nakanishi N,Mitsumoto M.Culture condition supporting adipocyte conversion of stromal-vascular cells from bovine intramuscullar adipocyte tissues[J].J Vet Med Sci,1996,58(2):1073-1078.
[22]梁杰,银桂彬,彭智,等.血管生成素协同血管内皮细胞生长因子对自体脂肪移植血运重建的影响[J].中国美容医学,2007,16(7):891-894.
[23]彭青和.瘦素影响SD大鼠移植颗粒脂肪成活率的实验研究[D].桂林:桂林医学院.2010.
[24]李锋,李昆,李杰,等.富血小板血浆对颗粒脂肪移植的影响[J].中国组织工程研究,2013,17(31):5645-5651.
[25]Craft RO,Rophael J,Morrison WA,et al.Effect of local,longterm delivery of platelet-derived growth factor(PDGF)on injected fat graft survival in severe combined immunodeficient(SCID)mice[J].J Plast Reconstr Aesthet Surg,2009,62(2):235-243.
[26]梁杰,赵坤,吴浩俊,等.重组人VEGF和前列腺素E1对移植脂肪早期血管生成的作用[J].中国实用医药,2009,4(11):7-9.
[27]Plate KH,Breier G,Weich HA,et al.Vascular endothelial growth factor is a potential tumour angiogenesis factor in human gliomas in vivo[J].Nature,1992,359(6398):845-848.
[28]付小兵,程飚,盛志勇.生长因子应用于临床创伤修复——十年的主要进展与展望[J].中国修复重建外科杂志,2004,18(6):508-512.
[29]黄双盛,吴肋杰.丹参的抗氧化与抗炎作用研究进展[J].中国中医药信息杂志,2002,9(1):86-87.
[30]李莉,刘耕陶.丹酚酸A对大鼠脑突触体和线粒体氧应激损伤的体外保护作用[J].中国药理学与毒理学杂志,1998,12(4):275-279.
[31]武承兴,王健,张波,等.黄芪对自体脂肪颗粒移植存活率的影响[J].组织工程与重建外科,2013,9(3):141-144.