复方中草药对山女鳟、白点鲑和亚东鲑肝脏抗氧化及非特异性免疫指标的影响
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摘要
在基础饲料中添加0.5%复方中草药,饲喂山女鳟、白点鲑、亚东鲑,并分别于第0、7、14、21、28 d检测其肝脏中丙二醛(MDA)含量、总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)活力、一氧化氮(NO)浓度、溶菌酶(LSZ)含量,以研究复方中草药对山女鳟、白点鲑、亚东鲑肝脏抗氧化及非特异性免疫的影响。结果表明,山女鳟肝脏中,T-SOD活力7 d时显著高于其他时间段(P<0.05),AKP活力7 d时显著高于0、21、28 d(P<0.05),NO含量和LSZ含量7 d时显著高于其他时间段(P<0.05)。白点鲑肝脏中,T-SOD、CAT、T-AOC活力14 d时显著高于0 d(P<0.05),ACP活力呈现升高后降低的趋势,7 d时升至最高且显著高于其他时间段(P<0.05)。亚东鲑肝脏中,T-SOD和T-AOC活力14 d时显著高于0 d(P<0.05),MDA含量14 d时显著低于其他时间段(P<0.05),AKP活力21 d时显著高于其他时间段(P<0.05);ACP活力7、14 d时显著高于其他时间段P<0.05),NO含量和LSZ含量14 d时显著高于0 d(P<0.05)。
0.5% Chinese herbals medicine compounds were supplemented to the basal formulated diet. Treatment groups were set up to feed Oncorhynchus masou masou and Salvelinus leucomaenisf pipiuvius and Salmo trutta fario. The content of MDA、T-SOD、CAT、T-AOC、AKP、ACP、NO、LSZ in serum liver were measured respectively on the 0, 7, 14, 21 and 28 d, with the view of studying on the influence of compound Chinese medicine to its antioxidation and nonspecific immunity. The results showed that the activity of T-SOD in the liver of Oncorhynchus masou masou was significantly higher with other time(P<0.05) on the 7 d. The activity of AKP on 7 d was significantly higher than that 0, 21 and 28 d(P<0.05). The content of NO and the activity of LSZ was significantly higher with other time(P<0.05). The activity of T-SOD, CAT, T-AOC on 14 d in the liver of Salvelinus leucomaenis significantly higher(P<0.05). The activity of ACP showed a trend of decrease after the increase and reached the highest 7 d. The activity of ACP was significantly higher than other time on 7 d(P<0.05).The activity of T-SOD and T-AOC in the liver of Salmo trutta fario was significantly higher than that on 0 d. The content of MDA was significantly lower with other time(P<0.05) on 14 d. The activity of AKP was significantly higher with other time(P<0.05) on the 21 th day. The activity of ACP was significantly higher with other time(P<0.05) on 7 and 14 d. The activity of LSZ and the content of NO was significantly higher with other time(P<0.05) on 14 d.
0.5% Chinese herbals medicine compounds were supplemented to the basal formulated diet. Treatment groups were set up to feed Oncorhynchus masou masou and Salvelinus leucomaenisf pipiuvius and Salmo trutta fario. The content of MDA、T-SOD、CAT、T-AOC、AKP、ACP、NO、LSZ in serum liver were measured respectively on the 0, 7, 14, 21 and 28 d, with the view of studying on the influence of compound Chinese medicine to its antioxidation and nonspecific immunity. The results showed that the activity of T-SOD in the liver of Oncorhynchus masou masou was significantly higher with other time(P<0.05) on the 7 d. The activity of AKP on 7 d was significantly higher than that 0, 21 and 28 d(P<0.05). The content of NO and the activity of LSZ was significantly higher with other time(P<0.05). The activity of T-SOD, CAT, T-AOC on 14 d in the liver of Salvelinus leucomaenis significantly higher(P<0.05). The activity of ACP showed a trend of decrease after the increase and reached the highest 7 d. The activity of ACP was significantly higher than other time on 7 d(P<0.05).The activity of T-SOD and T-AOC in the liver of Salmo trutta fario was significantly higher than that on 0 d. The content of MDA was significantly lower with other time(P<0.05) on 14 d. The activity of AKP was significantly higher with other time(P<0.05) on the 21 th day. The activity of ACP was significantly higher with other time(P<0.05) on 7 and 14 d. The activity of LSZ and the content of NO was significantly higher with other time(P<0.05) on 14 d.
引文
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[23]崔惟东,冷向军,李小勤,等.虾青素和角黄素对虹鳟肌肉着色和肝脏总抗氧化能力的影响[J].水产学报,2009(6):987-995.
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[28]王明华,彭刚,秦钦,等.饲料添加中草药制剂对日本沼虾免疫因子的影响[J].水产养殖,2008,29(2):8-10.
[29]王广军,谢骏,余德光,等.一氧化氮及一氧化氮合酶在水生动物免疫系统中的研究进展[J].水产科技,2009(3):1-6,13.
[30]孙裔雷,王荻,刘红柏.复方中草药对施氏鲟非特异性免疫功能的影响[J].中国农学通报,2015(8):45-49.
[31]苏岭,刘红柏,王荻,等.四种复方中药和黄芪多糖对鲫鱼生长、组织中NO含量与NOS活性的影响[J].水产学杂志,2010,23(3):11-15.
[32]吴旋.四种中草药多糖对黄颡鱼生长、体成分及部分生理生化指标的影响[D].天津:天津农学院,2011.
[33]Tort L,Balasch J C,Mackenzie S.Fish health challenge after stress.Indicators of immunocompetence[J].Contributionsto Science,2004,2:443-454.
[34]崔敏,郭冉,夏辉.用饲料酵母替代鱼粉对大菱鲆幼鱼生长及免疫机能的影响[J].大连海洋大学学报,2012(1):58-63.
[35]Zhang G,Gong S,Yu D,et al.Propolis and Herba Epimedii extracts enhance the non-specific immune response and disease resistance of Chinese sucker,Myxocyprinus asiaticus[J].Fish&Shellfish Immunology,2009,26(3):467-472.
[36]Tang J,Cai J,Liu R,et al.Immunostimulatory effects of artificial feed supplemented with a Chinese herbal mixture on Oreochromis niloticus against Aeromonas hydrophila[J].Fish&Shellfish Immunology,2014,39(2):401-406.
[2]陈术强.我国山女鳟引进群体遗传多样性评估及其改良[D].上海:上海海洋大学,2011.
[3]张永泉,白庆利,王炳谦,等.人工养殖的哲罗鱼、白点鲑和美洲红点鲑血液指标[J].中国农学通报,2011,32:56-60.
[4]沈钦一.不同饲料对白点鲑生长性能的影响[J].饲料广角,2016(7):42-44.
[5]豪富华.亚东鲑的生物学和遗传多样性研究[D].武汉:中国科学院研究生院(水生生物研究所),2006.
[6]王芳.亚东鲑若干养殖生物学特性研究[D].上海:上海海洋大学,2015.
[7]户国,王芳,周建设,等.野生亚东鲑群体遗传多样性及其种质来源研究[J].东北农业大学学报,2016(1):58-65.
[8]周凡,王亚琴.中草药多糖在水产中的研究进展[J].饲料与畜牧,2012(2):27-30.
[9]李金龙,胡毅,郇志利,等.复方中草药对黄鳝生长、非特异性免疫及肠道消化酶活性的影响[J].饲料工业,2013,34(16):26-30.
[10]张照红.复方中草药对罗非鱼、草鱼生长性能和非特异免疫功能的影响[D].福州:福建农林大学,2011.
[11]张耀武,郑建武,李文辉,等.复方中草药制剂对黄颡鱼生长和非特异性免疫功能的影响[J].水产科学,2010(4):225-228.
[12]吴卫星,申亮,张秀文,等.复方中草药提取物对鲤鱼非特异性免疫及生长性能的影响[J].江苏农业科学,2014(8):236-238.
[13]McCord J M.The evolution of free radicals and oxidative stress[J].Medicine,2000,108(8):652-659.
[14]黎瑞珍,杨庆建,陈贻锐.超氧化物歧化酶(SOD)活性的测定及其应用研究[J].琼州大学学报,2004,11(5):34-36.
[15]董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展[J].中国农业科技导报,2013(5):53-58.
[16]孙学亮,郭永军,季延滨,等.3种复方中草药对中华绒鳌蟹生长和部分抗氧化指标的影响[J].经济动物学报,2015(2):86-94.
[17]苏永腾,王恬.乳铁蛋白对鱼类的免疫增强作用[J].中国饲料,2006(12):28-29,34.
[18]张意敏.复方中草药和果寡糖对罗非鱼免疫性能的影响[D].上海:上海海洋大学,2013.
[19]吕耀平,成永旭,吴旭干,等.紫苏子提取物对异育银鲫存活、生长以及体生化组成的影响[J].水生生物学报,2008(2):258-266.
[20]梁拥军,孙向军,孙砚胜,等.复方中草药对宝石鲈抗氧化能力及免疫功能的影响[J].安徽农业科学,2009(15):7026-7029.
[21]Gu J,Gong J H,Yin Y B,et al.Effects of intermittent hyp-erbaric oxygen exposure and oxygen convulsions on a trioxidant enzyme activity and lipid peroxide contents in rats[J].Chinese Medical Journal,1995,2(4):31-34.
[22]朱慧文,张崇禧,马博谦,等.中药复方制剂对锦鲤生长、体色及若干生理生化指标的影响研究[J].淡水渔业,2011(3):9-14.
[23]崔惟东,冷向军,李小勤,等.虾青素和角黄素对虹鳟肌肉着色和肝脏总抗氧化能力的影响[J].水产学报,2009(6):987-995.
[24]Yoshida T,Kruger R,Inglis V.Augmentation of non-specific protection in African catfish,Clarias gariepinus(Burchell),by the long-term oral administration of immunostimulants[J].J.Fish Dis.,1995,18:195-198.
[25]刘志鸿,牟海津,王清印.软体动物免疫相关酶研究进展[J].海洋水产研究,2003(3):86-90.
[26]马维波.复方中草药对香鱼部分免疫生化指标的影响[D].长春:吉林农业大学,2007.
[27]刘丽平,薛晖,葛筱琴,等.复方中草药对青虾肌肉和肝胰脏中几种非特异性免疫因子的影响[J].淡水渔业,2007,37(6):11-14.
[28]王明华,彭刚,秦钦,等.饲料添加中草药制剂对日本沼虾免疫因子的影响[J].水产养殖,2008,29(2):8-10.
[29]王广军,谢骏,余德光,等.一氧化氮及一氧化氮合酶在水生动物免疫系统中的研究进展[J].水产科技,2009(3):1-6,13.
[30]孙裔雷,王荻,刘红柏.复方中草药对施氏鲟非特异性免疫功能的影响[J].中国农学通报,2015(8):45-49.
[31]苏岭,刘红柏,王荻,等.四种复方中药和黄芪多糖对鲫鱼生长、组织中NO含量与NOS活性的影响[J].水产学杂志,2010,23(3):11-15.
[32]吴旋.四种中草药多糖对黄颡鱼生长、体成分及部分生理生化指标的影响[D].天津:天津农学院,2011.
[33]Tort L,Balasch J C,Mackenzie S.Fish health challenge after stress.Indicators of immunocompetence[J].Contributionsto Science,2004,2:443-454.
[34]崔敏,郭冉,夏辉.用饲料酵母替代鱼粉对大菱鲆幼鱼生长及免疫机能的影响[J].大连海洋大学学报,2012(1):58-63.
[35]Zhang G,Gong S,Yu D,et al.Propolis and Herba Epimedii extracts enhance the non-specific immune response and disease resistance of Chinese sucker,Myxocyprinus asiaticus[J].Fish&Shellfish Immunology,2009,26(3):467-472.
[36]Tang J,Cai J,Liu R,et al.Immunostimulatory effects of artificial feed supplemented with a Chinese herbal mixture on Oreochromis niloticus against Aeromonas hydrophila[J].Fish&Shellfish Immunology,2014,39(2):401-406.