多指标综合评分法结合Box-Behnken响应面法优选心痛宁胶囊醇提工艺
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摘要
目的:采用多指标综合评分与Box-Behnken响应面法,优选心痛宁胶囊的醇提工艺。方法:以阿魏酸含量、总黄酮含量、总厚朴酚含量和浸膏得率的综合评分为指标,在单因素试验结果的基础上,运用Box-Behnken响应面法对影响醇提工艺的提取次数、时间、加醇浓度、加醇倍量进行考察研究,从而得到最优的醇提工艺。结果:最佳醇提工艺为加10倍量60%的乙醇,回流提取2次,每次提取1 h。结论:优选后的工艺方便、稳定、可行,可为该课题后续研究的开展提供参考。
Objective: Through the multi-index comprehensive evaluation method combined with the Box-Behnken response surface method to optimizing the alcohol extraction process of Xin Tong Ning capsule. Methods: The contents of ferulic acid flavonoids and honokiol,and extract yield were used as index,based on the results of single factor experiment,BoxBehnken response surface method was applied to optimize the ethanolic extraction process considering the extraction frequency,time,ethanol concentration. Results: The optimal parameters for ethanol extraction process were 10 times the amount of 60%ethanol,reflux extraction 2 times,each time 1 h. Conclusion: The optimized extractiong process is convenient,stable and feasible,can provide theoretical basis for the further study.
Objective: Through the multi-index comprehensive evaluation method combined with the Box-Behnken response surface method to optimizing the alcohol extraction process of Xin Tong Ning capsule. Methods: The contents of ferulic acid flavonoids and honokiol,and extract yield were used as index,based on the results of single factor experiment,BoxBehnken response surface method was applied to optimize the ethanolic extraction process considering the extraction frequency,time,ethanol concentration. Results: The optimal parameters for ethanol extraction process were 10 times the amount of 60%ethanol,reflux extraction 2 times,each time 1 h. Conclusion: The optimized extractiong process is convenient,stable and feasible,can provide theoretical basis for the further study.
引文
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[17]王永香,米慧娟,张传力,等. Box-Behnken响应面法优化热毒宁注射液金银花和青蒿(金青)的醇沉工艺研究[J].中草药,2015,46(5):671-678.
[18]夏米西奴尔·艾买提江,马建文,王萍. Box-Behnken响应面法优化白鲜皮解毒丸的提取工艺[J].中国药师,2018,21(4):611-616.
[19]李东,宗梁,郝福,等. Box-Behnken响应面法优选七味双花柴胡胶囊的水提工艺[J].中国现代中药,2018,20(1):88-92.
[20]李红伟,刘晴,孟祥乐,等. Box-Behnken响应面法优化葶苈-大枣药对提取工艺[J].中成药,2017,39(1):200-204.
[21]汪坤,赵旭,王楠斐,等. Box-Behnken法优选川乌、草乌、马钱子超声提取工艺[J].中国现代中药,2018,20(1):83-87+92.
[2]周鸿,黄含含,张静泽,等.川芎-当归药对研究进展[J].中成药,2015,37(1):184-188.
[3]罗年翠,丁雯,钱大玮,等.当归补血-活血药对功效取向研究[J].中国实验方剂学杂志,2013,19(4):240-246.
[4]李曦,张丽宏,王晓晓,等.当归化学成分及药理作用研究进展[J].中药材,2013,36(6):1023-1028.
[5]张淑洁,钟凌云.厚朴化学成分及其现代药理研究进展[J].中药材,2013,36(5):838-843.
[6]张文秀,朱卫丰,聂鹤云,等. Box-Behnken响应面法优化马钱子提取工艺[J].中成药,2015,37(9):1932-1936.
[7]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015:899-900
[8]刘旺培,黄义纯,陈华龙. HPLC法测定天王补心丸中阿魏酸的含量[J].中国药房,2015,26(21):3003-3005.
[9]李洋,季方茹,潘洪秀,等. HPLC法同时测定不同配伍比例三棱-莪术药对中P-香豆酸、阿魏酸的含量[J].中国药房,2017,28(27):3859-3861.
[10]高伟,朱叶珊,蔡春江,等.丁沉透膈丸中和厚朴酚、厚朴酚、白术内酯Ⅰ、白术内酯Ⅲ和儿茶素的测定[J].中成药,2014,36(5):980-985.
[11]杨秀娟,杨志军,牛鹏贤,等.甘肃不同产地红芪中总黄酮及总多糖含量测定研究[J].中国中医药信息杂志,2018,25(2):79-82.
[12]毛鑫,石开,王旭东,等.金针酸枣袋泡剂的质量标准研究[J].中华中医药学刊,2017,35(1):152-155.
[13]樊星,闫静,刘舒,等.刺五加根有效组分的抗氧化活性分析[J].中国实验方剂学杂志,2016,22(9):5-8.
[14]王萍,李茜,周颖,等.星点设计-效应面法优化苓柏解毒丸的提取工艺[J].中成药,2015,37(7):1617-1621.
[15]孙胜杰,郭亚菲,王艳,等.药对酸枣仁-远志总黄酮及总皂苷提取工艺的响应曲面试验优选[J].时珍国医国药,2018,29(3):568-571.
[16]吴璐,吴维刚,江亭,等.制川乌凝胶膏剂基质处方优化及体外释放研究[J].中国药房,2018,29(1):37-41.
[17]王永香,米慧娟,张传力,等. Box-Behnken响应面法优化热毒宁注射液金银花和青蒿(金青)的醇沉工艺研究[J].中草药,2015,46(5):671-678.
[18]夏米西奴尔·艾买提江,马建文,王萍. Box-Behnken响应面法优化白鲜皮解毒丸的提取工艺[J].中国药师,2018,21(4):611-616.
[19]李东,宗梁,郝福,等. Box-Behnken响应面法优选七味双花柴胡胶囊的水提工艺[J].中国现代中药,2018,20(1):88-92.
[20]李红伟,刘晴,孟祥乐,等. Box-Behnken响应面法优化葶苈-大枣药对提取工艺[J].中成药,2017,39(1):200-204.
[21]汪坤,赵旭,王楠斐,等. Box-Behnken法优选川乌、草乌、马钱子超声提取工艺[J].中国现代中药,2018,20(1):83-87+92.