基于苯并噻唑腙的铜离子荧光探针

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英文篇名：A Turn-on Fluorescent Probe for Cu~(2+) Based on Benzothiazole-2-ylhydrazone 作者：李清泉 ; 沈静 ; 唐灿 ; 张征宇 ; 王恩举 英文作者：LI Qingquan;SHEN Jing;TANG Can;ZHANG Zhengyu;WANG Enju;College of Chemistry and Chemical Engineering,Hainan Normal University; 关键词：荧光探针 ; 2 ; 4-二甲氧基苯甲醛 ; 2-肼基苯并噻唑 ; 铜离子 英文关键词：fluorescent probe;;2-hydrazinylbenzothiazole;;2,4-dimethoxybenzaldehyde;;copper ion 中文刊名：HNXZ 英文刊名：Journal of Hainan Normal University(Natural Science) 机构：海南师范大学化学与化工学院; 出版日期：2019-03-15 出版单位：海南师范大学学报(自然科学版) 年：2019 期：v.32 基金：海南省自然科学基金(20162028);; 大学生创新训练计划项目 语种：中文; 页：HNXZ201901007 页数：4 CN：01 ISSN：46-1075/N 分类号：42-45

摘要

铜是生命必需微量元素,是许多金属酶发挥催化作用的辅因子,同时,过量摄入铜能引起许多神经退行性疾病。因此,方便、快速地检测铜离子非常有价值。通过缩合2,4-二甲氧基苯甲醛与2-肼基苯并噻唑合成了一个可以高选择性识别铜离子的荧光探针,反应简单,产率较高,具有一定的实用价值。在乙腈/HEPES缓冲溶液中(pH=7.0),探针没有荧光,加入铜离子能引起发射强度显著增加,发出明亮的蓝色荧光,其它常见金属离子不能引起探针的发射光谱发生明显变化,而且对铜离子的检测没有影响。Job曲线研究表明二者形成了1:1的络合物。
        Copper is an essential trace element necessary for all organisms. It plays an important role as a catalytic cofactor for a broad range of metalloenzymes. Nevertheless, copper overload is associated with many neurodegenerative diseases including Alzheimer diseases. A novel fluorescent probe was developed by incorporating 2,4-dimethoxybenzaldehyde and 2-hydrazinylbenzo [d] thiazole for the detection of Cu~(2+). In MeCN/HEPES buffer solution, the probe exhibited a pronounced Cu~(2+)-selective fluorescence enhancement over other common metal ions. The bright blue fluorescence provides the nakedeye detection of Cu~(2+). The 1:1 stochiomety of the complex was determined by Job's plot.

引文

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