支原体肺炎患儿血清sB7-H3、IFN-γ、GM-CSF的变化观察
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摘要
目的探讨血清共刺激分子可溶性B7-H3(s B7-H3)、干扰素-γ(INF-γ)及粒细胞巨噬细胞刺激因子(GM-CSF)在肺炎支原体肺炎(MP)患儿体内变化的意义。方法选取确诊MP患儿120例(MP组)、选取同期健康儿童120例(对照组),检测两组的血清sB7-H3、IFN-γ、GM-CSF水平,依据MP患儿病情分为重症组、轻症组,进行亚组分析,采用线性相关分析法探索重症MP患儿血清sB7-H3、IFN-γ、GM-CSF水平与儿童危重病例评分(PCIS)的关系。结果 MP组患儿的血清sB7-H3、IFN-γ、GM-CSF水平均显著高于对照组,差异具有统计学意义(P<0.05);重症组MP患儿的血清sB7-H3、IFN-γ、GM-CSF水平均显著高于轻症组,差异具有统计学意义(P<0.05);重症组MP患儿的PCIS评分均显著低于轻症组,差异具有统计学意义(P<0.05);重症组MP患儿的PCIS评分与血清sB7-H3、IFN-γ、GM-CSF水平均呈显著的负相关关系(P<0.05)。结论哮喘患儿血清sB7-H3、IFN-γ、GM-CSF水平较健康患儿显著升高,与患儿病情严重程度具有密切关系。
Objective To investigate the changes of serum costimulatory molecules soluble B7-H3(s B7-H3), interferon-γ(INF-γ) and granulocyte macrophage stimulating factor(GM-CSF) in children with Mycoplasma pneumoniae pneumonia(MP). Methods 120 children with confirmed MP(the MP group) and 120 healthy children(the control group) were selected. Their serum sB7-H3, IFN-γ, and GM-CSF were detected in the two groups. According to the condition of MP children, they were divided into the severe group and the mild group for subgroup analysis. Linear correlation analysis was used to explore serum sB7-H3, IFN-γ, GM-CSF levels and critical illness scores in children with severe MP(PCIS). Results The serum levels of sB7-H3, IFN-γ and GM-CSF in the MPgroup were significantly higher than those in the control group( P < 0. 05),and they were significantly higher in the severe group than in the mild group( P < 0. 05). The PCIS scores were significantly lower in the severe group than in the mild group( P < 0. 05). PCIS scores in children with severe MP were significantly negatively correlated with serum s B7-H3,IFN-γ,and GM-CSF( P < 0. 05). Conclusion Serum levels of s B7-H3,IFN-γ and GM-CSF are significantly higher in children with asthma than in healthy children,which are closely related to the severity of the disease.
Objective To investigate the changes of serum costimulatory molecules soluble B7-H3(s B7-H3), interferon-γ(INF-γ) and granulocyte macrophage stimulating factor(GM-CSF) in children with Mycoplasma pneumoniae pneumonia(MP). Methods 120 children with confirmed MP(the MP group) and 120 healthy children(the control group) were selected. Their serum sB7-H3, IFN-γ, and GM-CSF were detected in the two groups. According to the condition of MP children, they were divided into the severe group and the mild group for subgroup analysis. Linear correlation analysis was used to explore serum sB7-H3, IFN-γ, GM-CSF levels and critical illness scores in children with severe MP(PCIS). Results The serum levels of sB7-H3, IFN-γ and GM-CSF in the MPgroup were significantly higher than those in the control group( P < 0. 05),and they were significantly higher in the severe group than in the mild group( P < 0. 05). The PCIS scores were significantly lower in the severe group than in the mild group( P < 0. 05). PCIS scores in children with severe MP were significantly negatively correlated with serum s B7-H3,IFN-γ,and GM-CSF( P < 0. 05). Conclusion Serum levels of s B7-H3,IFN-γ and GM-CSF are significantly higher in children with asthma than in healthy children,which are closely related to the severity of the disease.
引文
[1] LEE E,CHO H J,HONG S J,et al.Prevalence and clinical manifestations of macrolide resistant Mycoplasma pneumoniae pneumonia in Korean children[J].Korean J Pediatr,2017,60(5):151-157.
[2] DASH S,CHAUDHRY R,DHAWAN B,et al.Clinical spectrum and diagnostic yields of Mycoplasma pneumoniae as a causative agent of community-acquired pneumonia[J].J Lab Physicians,2018,10(1):44-49.
[3] YIN Y D,WANG R,ZHUO C,et al.Macrolide-resistant Mycoplasma pneumoniae prevalence and clinical aspects in adult patients with community-acquired pneumonia in China:a prospective multicenter surveillance study[J].J Thorac Dis,2017,9(10):3774-3781.
[4] 戴贞照.sB7-H3和IFN-γ在儿童支原体肺炎中的表达水平及临床意义[J].赣南医学院学报,2017,37(2):224-226,238.
[5] 任一帆,银羽,张传新,等.34例支原体肺炎患儿血清干扰素-γ、白细胞介素-6、白细胞介素-8及乳酸脱氢酶的检测及临床意义[J].中国卫生检验杂志,2017,27(23):3411-3413.
[6] 马芬,毕勇志,王芳.支原体肺炎患者中性粒细胞碱性磷酸酶、急性时相反应蛋白及细胞因子检测的临床意义[J].中国实验诊断学,2018,22(1):6-9.
[7] 黄静,代永红.难治性新生儿肺炎危险因素分析[J].现代仪器与医疗,2016,22(3):65-66,88.
[8] SARAYA T,OHKUMA K,TSUKAHARA Y,et al.Correlation between clinical features,high-resolution computed tomography findings,and a visual scoring system in patients with pneumonia due to Mycoplasma pneumoniae[J].Respir Investig,2018,56(4):320-325.
[9] HAN M S,YUN K W,LEE H J,et al.Contribution of Co-detected Respiratory Viruses and Patient Age to the Clinical Manifestations of Mycoplasma Pneumoniae Pneumonia in Children[J].Pediatr Infect Dis J,2018,37(6):531-536.
[10] 杨晓勇,姚庆春,王玮,等.树突状细胞共刺激分子表达与免疫抑制剂的干预[J].中国组织工程研究,2015,19(24):3876-3881.
[11] 陈丹,孙晓敏,郭青.肺炎支原体肺炎患儿血清IL-6、IFN-γ的变化及意义[J].中国现代药物应用,2016,10(2):16-17.
[12] 周红,徐庆雷,马小波,等.粒细胞巨噬细胞集落刺激因子、髓过氧化物酶在肺炎支原体肺炎患儿支气管肺泡灌洗液中的表达及其临床意义[J].中华实用儿科临床杂志,2017,32(4):313-315.
[13] 林涛,孔雅娴,贾蓓,等.脂多糖所致脓毒症诱导的急性肺损伤肺组织T淋巴细胞活化及共刺激分子表达的研究[J/OL].中华实验和临床感染病杂志(电子版),2015,9(2):116-119[2018-11-19].http://med.wanfangdata.com.cn/Paper/Detail?id=PeriodicalPaper_zhsyhlcgrbzz201502031&dbid=WF_QK.DOI:10.3877/cma.j.issn.1674-1358.2015.02.031.
[14] 梁春莉,郝晓燕,杨生梅.肺炎支原体肺炎患儿血清干扰素-γ白细胞介素-6及白三烯水平的变化[J].中国药物与临床,2016,16(7):972-973.
[2] DASH S,CHAUDHRY R,DHAWAN B,et al.Clinical spectrum and diagnostic yields of Mycoplasma pneumoniae as a causative agent of community-acquired pneumonia[J].J Lab Physicians,2018,10(1):44-49.
[3] YIN Y D,WANG R,ZHUO C,et al.Macrolide-resistant Mycoplasma pneumoniae prevalence and clinical aspects in adult patients with community-acquired pneumonia in China:a prospective multicenter surveillance study[J].J Thorac Dis,2017,9(10):3774-3781.
[4] 戴贞照.sB7-H3和IFN-γ在儿童支原体肺炎中的表达水平及临床意义[J].赣南医学院学报,2017,37(2):224-226,238.
[5] 任一帆,银羽,张传新,等.34例支原体肺炎患儿血清干扰素-γ、白细胞介素-6、白细胞介素-8及乳酸脱氢酶的检测及临床意义[J].中国卫生检验杂志,2017,27(23):3411-3413.
[6] 马芬,毕勇志,王芳.支原体肺炎患者中性粒细胞碱性磷酸酶、急性时相反应蛋白及细胞因子检测的临床意义[J].中国实验诊断学,2018,22(1):6-9.
[7] 黄静,代永红.难治性新生儿肺炎危险因素分析[J].现代仪器与医疗,2016,22(3):65-66,88.
[8] SARAYA T,OHKUMA K,TSUKAHARA Y,et al.Correlation between clinical features,high-resolution computed tomography findings,and a visual scoring system in patients with pneumonia due to Mycoplasma pneumoniae[J].Respir Investig,2018,56(4):320-325.
[9] HAN M S,YUN K W,LEE H J,et al.Contribution of Co-detected Respiratory Viruses and Patient Age to the Clinical Manifestations of Mycoplasma Pneumoniae Pneumonia in Children[J].Pediatr Infect Dis J,2018,37(6):531-536.
[10] 杨晓勇,姚庆春,王玮,等.树突状细胞共刺激分子表达与免疫抑制剂的干预[J].中国组织工程研究,2015,19(24):3876-3881.
[11] 陈丹,孙晓敏,郭青.肺炎支原体肺炎患儿血清IL-6、IFN-γ的变化及意义[J].中国现代药物应用,2016,10(2):16-17.
[12] 周红,徐庆雷,马小波,等.粒细胞巨噬细胞集落刺激因子、髓过氧化物酶在肺炎支原体肺炎患儿支气管肺泡灌洗液中的表达及其临床意义[J].中华实用儿科临床杂志,2017,32(4):313-315.
[13] 林涛,孔雅娴,贾蓓,等.脂多糖所致脓毒症诱导的急性肺损伤肺组织T淋巴细胞活化及共刺激分子表达的研究[J/OL].中华实验和临床感染病杂志(电子版),2015,9(2):116-119[2018-11-19].http://med.wanfangdata.com.cn/Paper/Detail?id=PeriodicalPaper_zhsyhlcgrbzz201502031&dbid=WF_QK.DOI:10.3877/cma.j.issn.1674-1358.2015.02.031.
[14] 梁春莉,郝晓燕,杨生梅.肺炎支原体肺炎患儿血清干扰素-γ白细胞介素-6及白三烯水平的变化[J].中国药物与临床,2016,16(7):972-973.