副鸡禽杆菌发酵培养工艺研究
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摘要
本试验从接种方式、培养时间、初始接种量等方面对副鸡禽杆菌C-Apg-8(A型)的发酵培养条件进行了工艺改进。结果显示,通过将现有副鸡禽杆菌生产的二步接种法(一级种子→二级种子(7 500mL)→300L)改为四步接种法(4mL→160mL→1 600mL→30 000mL→300L),接种比例由之前的2.5%变为2.5%~10%,培养方式由大瓶培养变为发酵罐培养,同时在不改变单批次发酵总体积、不增加人员和原材料成本的情况下,最终可以使副鸡禽杆菌发酵单批产量增加约5倍。
The fermentation cultivation process of Avibacterium paragallinarum C-Apg-8(serovar A)had been improved in terms of inoculation method,culture time,the initial inoculation quantity of microorganism and so on.The results showed that the single-batch production capacity of Avibacterium paragallinarum fermentation could increase almost five times by changing the present two-step inoculation method(the primary seed→the secondary seed(7 500mL)→300L)to the four-step inoculation method(4mL→160mL→1 600mL→30 000mL→300L),and adjusting the inoculation proportion from 2.5%to 2.5%-10%.The improved cultivation way had been changed from spinner cultivation to fermenter cultivation without changing the volume of fermentation,and without adding the staff and the cost of raw material.
The fermentation cultivation process of Avibacterium paragallinarum C-Apg-8(serovar A)had been improved in terms of inoculation method,culture time,the initial inoculation quantity of microorganism and so on.The results showed that the single-batch production capacity of Avibacterium paragallinarum fermentation could increase almost five times by changing the present two-step inoculation method(the primary seed→the secondary seed(7 500mL)→300L)to the four-step inoculation method(4mL→160mL→1 600mL→30 000mL→300L),and adjusting the inoculation proportion from 2.5%to 2.5%-10%.The improved cultivation way had been changed from spinner cultivation to fermenter cultivation without changing the volume of fermentation,and without adding the staff and the cost of raw material.
引文
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[2]冯文达.北京鸡传染性鼻炎病原菌的分离鉴定[J].微生物学通报,1987,5:216-219.
[3]杨国良,郑杰,张洪,等.2株B型副鸡禽杆菌的分离鉴定[J].中国兽药杂志,2013,47(3):14-17.
[4]杨国良,郑杰,张发明,等.A型副鸡嗜血杆菌(山东株)的分离与鉴定[J].中国畜牧兽医,2013,40(2):189-192.
[5]张洪,王文泉,郭致君,等.副鸡嗜血杆菌在疫苗生产中生长特性的研究[J].中国兽药杂志,2003,37(5):15-17.
[6]中华人民共和国农业部兽用生物制品规程委员会.中华人民共和国兽用生物制品规程[S].北京:化学工业出版社,2000.
[7]苗立中.副猪嗜血杆菌荧光定量PCR方法建立及其分离株高密度发酵研究[D].长春:吉林大学,2012.
[8]沈旭.副鸡嗜血杆菌HB株的分离、鉴定及其高密度发酵的研究[D].长春:吉林大学,2013.
[9]李季伦,张伟心,杨启瑞.微生物生理学[M].北京:北京农业大学出版社,1993.
[10]中华人民共和国农业部.中华人民共和国兽用生物制品质量标准[S].北京:中国农业科技出版社,2001.