痧脊经筋疗法对兔膝骨性关节炎软骨组织及细胞因子的影响
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摘要
目的:研究痧脊经筋疗法对兔膝骨性关节炎软骨的影响,探讨非药物治疗膝骨性关节炎的作用机制。方法:采用管型石膏过伸位制动兔左后膝骨关节,并按照随机数字化法分组,造模2个月后开始痧脊经筋治疗,2个月后将兔处死,取材进行大体观察、Mankin评分,实时荧光定量PCR检测软骨TGF-β1、Smad3 mRNA,ELISA法检测关节液白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)。结果:痧脊经筋组和氨基葡萄糖组软骨TGF-β1、Smad3 mRNA高于模型对照组(P<0.05),关节液中IL-1β、IL-6、TNF-α的水平低于模型对照组(P<0.05)。与氨基葡萄糖组比较,痧脊经筋组TGF-β1、Smad3 mRNA较高(P<0.05)。结论:痧脊经筋疗法可使软骨退变的部分因素得到控制,是值得推广的新型非药物治疗方法。
Objective: To study 'scraping spinal-acupoints and meridian-muscle' therapy on knee osteoarthritis in rabbits,and explore the mechanism of non-drug treatment of knee osteoarthritis. Methods: The rabbit's left posterior knee joint was immobilized by hyperextension of tubular plaster and was grouped according to random digitization. The rabbits were sacrificed after 2 months. The rabbits were observed and scored by Mankin. The mRNA of TGF-β1 and Smad3 in cartilage and the levels of IL-1β, IL-6 and TNF-α in joint fluid were detected. Results: TGF-β1 and Smad3 in scraping spinal-acupoints and glucosamine group cartilage were higher than model control group(P<0.05). The levels of IL-1β, IL-6 and TNF-α in synovial fluid were lower than model control group(P<0.05). The expressiones of TGF-β1 and Smad3 mRNA in glucosamine group were lower than scraping spinal-acupoints and meridian-muscle group(P<0.05). Conclusion: Scraping spinal-acupoints and meridian-muscle therapy can control some factors of cartilage degeneration, it is a new non-drug treatment method which is worth popularizing.
Objective: To study 'scraping spinal-acupoints and meridian-muscle' therapy on knee osteoarthritis in rabbits,and explore the mechanism of non-drug treatment of knee osteoarthritis. Methods: The rabbit's left posterior knee joint was immobilized by hyperextension of tubular plaster and was grouped according to random digitization. The rabbits were sacrificed after 2 months. The rabbits were observed and scored by Mankin. The mRNA of TGF-β1 and Smad3 in cartilage and the levels of IL-1β, IL-6 and TNF-α in joint fluid were detected. Results: TGF-β1 and Smad3 in scraping spinal-acupoints and glucosamine group cartilage were higher than model control group(P<0.05). The levels of IL-1β, IL-6 and TNF-α in synovial fluid were lower than model control group(P<0.05). The expressiones of TGF-β1 and Smad3 mRNA in glucosamine group were lower than scraping spinal-acupoints and meridian-muscle group(P<0.05). Conclusion: Scraping spinal-acupoints and meridian-muscle therapy can control some factors of cartilage degeneration, it is a new non-drug treatment method which is worth popularizing.
引文
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[11]李西海,刘献祥.基于筋骨理论探讨膝骨关节炎筋骨失衡的治疗策略.中华中医药杂志,2017,32(8):3344-3346
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[14]刘镕,赵琴平,董惠芬,等.TGF-β信号传导通路及其生物学功能.中国病原生物学杂志,2014,9(1):77-81
[15]Eritja,Nuria,Felip,et al.A Smad3-PTEN regulatory loop controls proliferation and apoptotic responses to TGF-[beta] in mouse endometrium.Cell Death and Differentiation,2017,24(8):1443-1458
[16]黄媛霞,徐海斌,郭春.白细胞介素-1β、肿瘤坏死因子α及基质金属蛋白酶13在骨性关节炎中的表达及相关性.广东医学,2017,38(15):2301-2304
[2]潘建科,洪坤豪,刘军,等.补肾活血中药治疗膝骨关节炎有效性和安全性的系统评价.中华中医药杂志,2016,31(12):5248-5256
[3]孙宁,李永婷,林璐璐,等.不同针灸疗法治疗膝骨关节炎的比较与分析.中华中医药杂志,2017,32(7):3253-3255
[4]邓健,刘渊,杨立强.痧脊经筋疗法治疗膝骨关节炎的临床研究.时珍国医国药,2017,28(5):1130-1132
[5]曾俊华,马笃军,彭力平,等.实验兔膝骨关节炎模型的建立及鉴定.中国临床研究,2016,29(5):679-682
[6]Szychlinska M A,Trovato F M,Di R M,et al.Co-expression and Co-localization of cartilage glycoproteins CHI3L1 and lubricin in osteoarthritic cartilage:Morphological,immunohistochemical and geng expression profiles.Int J Mol Sci,2016,17(3):359
[7]Sgamma T,Pape J,Massiah A,et al.Selection of reference genes for diurnal and developmental time-course real-time PCR expression analyses in lettuce.Plant Methods,2016(12):21
[8]Bruyere O,Altman R D,Reginster J Y.Efficacy and safety of glucosamine sulfate in the management of osteoarthritis:Evidence from real-life setting trials and surveys.Semin Arthritis Rheum,2016,45(Suppl 4):S12-S17
[9]郭蕾,陈以国.经筋理论和经筋疗法的历史回顾.中华中医药学刊,2011,29(1):169-171
[10]赵勇,秦伟凯,顾力军.从经筋论治膝骨关节炎疼痛临证思维.中国中医药信息杂志,2012,19(3):169-171
[11]李西海,刘献祥.基于筋骨理论探讨膝骨关节炎筋骨失衡的治疗策略.中华中医药杂志,2017,32(8):3344-3346
[12]罗大伦.阴阳一调百病消.北京:中华工商联合出版社,2012:225
[13]赵旭晨,赵畅,曾春,等.小鼠膝关节骨关节炎细胞因子的表达.福建医科大学学报,2016,50(3):143-147
[14]刘镕,赵琴平,董惠芬,等.TGF-β信号传导通路及其生物学功能.中国病原生物学杂志,2014,9(1):77-81
[15]Eritja,Nuria,Felip,et al.A Smad3-PTEN regulatory loop controls proliferation and apoptotic responses to TGF-[beta] in mouse endometrium.Cell Death and Differentiation,2017,24(8):1443-1458
[16]黄媛霞,徐海斌,郭春.白细胞介素-1β、肿瘤坏死因子α及基质金属蛋白酶13在骨性关节炎中的表达及相关性.广东医学,2017,38(15):2301-2304