酶法-超声提取黄精总黄酮及其抗氧化活性研究
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摘要
目的研究酶法-超声提取黄精中总黄酮(total flavonoids from Polygonatum sibirici,TFPS)的最佳工艺及总黄酮的体外抗氧化活性。方法以总黄酮含量为指标,采用单因素和正交试验对纤维素酶用量、乙醇浓度、液料比和超声时间进行考察,优化工艺条件并考察总黄酮对DPPH自由基、ABTS+自由基的清除能力和亚铁离子螯合活性。结果最佳提取条件:纤维素酶用量为0.75%,乙醇浓度为40%,液料比为20mL·g~(-1),超声时间30min,总黄酮含量为1.595%。体外抗氧化试验表明,黄精总黄酮对DPPH自由基、ABTS+自由基有很强的清除能力,IC50分别为27.55,11.47μg·mL~(-1),对亚铁离子的螯合活性较强,IC50为32.26μg·mL~(-1)。结论酶法-超声提取工艺具有提取效率高、稳定性及重复性好等优点,可用于黄精中总黄酮的提取,在该工艺下提取的总黄酮具有较强的抗氧化活性。
OBJECTIVE To study the optimum extraction technology for the total flavonoids from Polygonatum sibiricum(TFPS) by enzymatic-ultrasonic method, and to research its antioxidant activities. METHODS Using total flavonoids compounds as investigation indexes, single factor and orthogonal test were used to investigate the cellulase amount, ethanol concentration, liquid-solid ratio and ultrasonic time, and the extraction technology was optimized. Furthermore, the antioxidant activities of TFPS were evaluated, using various antioxidant assays included the scavenging on DPPH, ABTS+ and ferrous ion-chelating capacity. RESULTS The optimum extraction conditions were as follows: cellulase amount was 0.75%, ethanol concentration was 40%, liquid-solid ratio was 20 mL·g(-1), ultrasonic time was 30 min, and extraction rate of total flavonoids was1.595%. TFPS had strong scavenging activity on ·DPPH and ABTS+ free radicals, the IC50 was 27.55, 11.47 μg·mL~(-1)? and also had strong ferrous ion-chelating capacity, the IC50 was 32.26 μg·mL~(-1). CONCLUSION The enzymatic-ultrasonic method is stable, feasible and has the advantage of high extraction efficiency, and can be used for the extraction of TFPS. These results show that TFPS has strong antioxidant activity under the optimal extraction technology.
OBJECTIVE To study the optimum extraction technology for the total flavonoids from Polygonatum sibiricum(TFPS) by enzymatic-ultrasonic method, and to research its antioxidant activities. METHODS Using total flavonoids compounds as investigation indexes, single factor and orthogonal test were used to investigate the cellulase amount, ethanol concentration, liquid-solid ratio and ultrasonic time, and the extraction technology was optimized. Furthermore, the antioxidant activities of TFPS were evaluated, using various antioxidant assays included the scavenging on DPPH, ABTS+ and ferrous ion-chelating capacity. RESULTS The optimum extraction conditions were as follows: cellulase amount was 0.75%, ethanol concentration was 40%, liquid-solid ratio was 20 mL·g(-1), ultrasonic time was 30 min, and extraction rate of total flavonoids was1.595%. TFPS had strong scavenging activity on ·DPPH and ABTS+ free radicals, the IC50 was 27.55, 11.47 μg·mL~(-1)? and also had strong ferrous ion-chelating capacity, the IC50 was 32.26 μg·mL~(-1). CONCLUSION The enzymatic-ultrasonic method is stable, feasible and has the advantage of high extraction efficiency, and can be used for the extraction of TFPS. These results show that TFPS has strong antioxidant activity under the optimal extraction technology.
引文
[1]中国药典.一部[S].2015:306-307.
[2]陈丽霞,王建科,耿晓玲.综合评分法优选黄精蒸制工艺[J].微量元素与健康研究,2017,34(5):38-40.
[3]YU H,ZHANG X P,DENG M Q,et al.Study on constituents and biological activity of volatile oil from tubers of Polygonatum cyrtonema Hua[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2008,14(5):4-6.
[4]WANG W J,XIANG C H,LIU C H.Extraction and properties analysis of total flavonoids of Rhizoma Polygonati[J].Food Indust(食品工业),2014,35(10):258-262.
[5]CHEN Y J,SHI X,QU R,et al.Study on extraction process of flavonoids from Polygonatum kingianum Coll.et Hemsl and antioxidative activities of flavonoids[J].Sci Technol Food Indust(食品工业科技),2013,34(5):222-225.
[6]SU Y J,NI G F.Determination of the content and method of extracting total flavonoids in Herba Cirsii[J].Zhejiang JTradit Chin Med(浙江中医杂志),2013,48(2):143-144.
[7]陈克克,史丽,李莺,等.黄精总黄酮和总酚的含量测定[J].陕西农业科学,2012(1):58-60.
[8]ZHANG J J,ZHANG R,NING W.The method research of Polygonatum macropodium Turcz content and total flavonoids by ultrasonic extraction[J].Lishizhen Med Mater Med Res(时珍国医国药),2015,26(12):2870-2871.
[9]LI L,LI L,QIU X,et al.Study on extraction of total flavonoids from microwave-assisted route[J].J Baoshan Teach Coll(保山学院学报),2014,33(5):39-42.
[10]LI Y H,MA Y H,HUANG K H,et al.Extraction of Aloe Polysaccharides by cellulase assisted with ultrasonic and studies on antitumor activity[J].J Chin Ins Food Sci Technol(中国食品学报),2015,15(11):91-97.
[11]PAN D M,LI X R.Optimization of ultrasonic assisted extraction of total flavonoids from Semen Plantaginis[J].Zhejiang J Tradit Chin Med(浙江中医杂志),2014,49(12):924-925.
[12]SHAO S J,LI X,WEI J L.Extraction of total flavonoids from Pinus massoniana pollen by ultrasonic assisted cellulase[J].Chin Tradit Pat Med(中成药),2016,38(1):204-207.
[13]CHU W,LI L L,LI Y,et al.Extraction process and antioxidation activities of Cortex Dictamni polysaccharide[J].Chin Arch Tradit Chin Med(中华中医药学刊),2017,35(7):1809-1812.
[14]XIA D Z,YU X F,ZHU Z Y,et al.Antioxidant and antibacterialactivity of six edible wild plants(Sonchus spp.)in China[J].Nat Prod Res,2011,25(20):1893-1901.
[15]LIU Q,XIA D Z,YE X P,et al.Processing and antioxidant activities of anjibaichatea polyphenols tablets[J].Sci Technol Food Indust(食品工业科技),2012,33(18):245-247,252.
[2]陈丽霞,王建科,耿晓玲.综合评分法优选黄精蒸制工艺[J].微量元素与健康研究,2017,34(5):38-40.
[3]YU H,ZHANG X P,DENG M Q,et al.Study on constituents and biological activity of volatile oil from tubers of Polygonatum cyrtonema Hua[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2008,14(5):4-6.
[4]WANG W J,XIANG C H,LIU C H.Extraction and properties analysis of total flavonoids of Rhizoma Polygonati[J].Food Indust(食品工业),2014,35(10):258-262.
[5]CHEN Y J,SHI X,QU R,et al.Study on extraction process of flavonoids from Polygonatum kingianum Coll.et Hemsl and antioxidative activities of flavonoids[J].Sci Technol Food Indust(食品工业科技),2013,34(5):222-225.
[6]SU Y J,NI G F.Determination of the content and method of extracting total flavonoids in Herba Cirsii[J].Zhejiang JTradit Chin Med(浙江中医杂志),2013,48(2):143-144.
[7]陈克克,史丽,李莺,等.黄精总黄酮和总酚的含量测定[J].陕西农业科学,2012(1):58-60.
[8]ZHANG J J,ZHANG R,NING W.The method research of Polygonatum macropodium Turcz content and total flavonoids by ultrasonic extraction[J].Lishizhen Med Mater Med Res(时珍国医国药),2015,26(12):2870-2871.
[9]LI L,LI L,QIU X,et al.Study on extraction of total flavonoids from microwave-assisted route[J].J Baoshan Teach Coll(保山学院学报),2014,33(5):39-42.
[10]LI Y H,MA Y H,HUANG K H,et al.Extraction of Aloe Polysaccharides by cellulase assisted with ultrasonic and studies on antitumor activity[J].J Chin Ins Food Sci Technol(中国食品学报),2015,15(11):91-97.
[11]PAN D M,LI X R.Optimization of ultrasonic assisted extraction of total flavonoids from Semen Plantaginis[J].Zhejiang J Tradit Chin Med(浙江中医杂志),2014,49(12):924-925.
[12]SHAO S J,LI X,WEI J L.Extraction of total flavonoids from Pinus massoniana pollen by ultrasonic assisted cellulase[J].Chin Tradit Pat Med(中成药),2016,38(1):204-207.
[13]CHU W,LI L L,LI Y,et al.Extraction process and antioxidation activities of Cortex Dictamni polysaccharide[J].Chin Arch Tradit Chin Med(中华中医药学刊),2017,35(7):1809-1812.
[14]XIA D Z,YU X F,ZHU Z Y,et al.Antioxidant and antibacterialactivity of six edible wild plants(Sonchus spp.)in China[J].Nat Prod Res,2011,25(20):1893-1901.
[15]LIU Q,XIA D Z,YE X P,et al.Processing and antioxidant activities of anjibaichatea polyphenols tablets[J].Sci Technol Food Indust(食品工业科技),2012,33(18):245-247,252.