布鲁氏菌胞内存活及疫苗研究进展
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摘要
布病是当前最为流行的细菌性人兽共患传染病,因导致家畜流产可引发重大经济损失,也是发展中国家面临的最为重要的公共卫生问题。阐明布鲁氏菌的致病机制是设计和研发疫苗的关键,而疫苗免疫是预防布病较为有效的方法。但目前无安全有效的人用疫苗,唯一的预防策略是动物免疫,但每年的人间和动物发病率仍然较高,动物免疫可能存在缺陷。当前,布鲁氏菌的多种免疫蛋白已被鉴定、评价,新型亚单位疫苗也表现出了良好的免疫潜能。因此,本文就布鲁氏菌的胞内生存机制、国内外常见疫苗的应用研究情况进行综述,为开展相关研究提供有价值的参考。
Brucellosis is one of the most prevalent bacterial zoonotic disease causing significant economic losses due to the livestock abortion,it is also a most grave public health problem in many developing countries.Elucidate the pathogenesis mechanism of Brucellaspp.is a crucial for vaccine research and development,and vaccine immunization is an effectiveness measures to precaution Brucellosis.At present,there is no safe and protection human vaccine against brucellosis,the only preventive strategy is animal vaccination,but the annual incidence of human and animal brucellosis is still high,which harbors significant disadvantages.Recently,many immune proteins of Brucella were detected and evaluated,and some new subunit vaccine showing excellent immune potential.This review represents an overview of brucellosis in intracelluar survival and vaccine research advance,providing a valuable reference for related research.
Brucellosis is one of the most prevalent bacterial zoonotic disease causing significant economic losses due to the livestock abortion,it is also a most grave public health problem in many developing countries.Elucidate the pathogenesis mechanism of Brucellaspp.is a crucial for vaccine research and development,and vaccine immunization is an effectiveness measures to precaution Brucellosis.At present,there is no safe and protection human vaccine against brucellosis,the only preventive strategy is animal vaccination,but the annual incidence of human and animal brucellosis is still high,which harbors significant disadvantages.Recently,many immune proteins of Brucella were detected and evaluated,and some new subunit vaccine showing excellent immune potential.This review represents an overview of brucellosis in intracelluar survival and vaccine research advance,providing a valuable reference for related research.
引文
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[16] Celli J.Surviving inside a macrophage:the many ways of Brucella[J].Res Microbiol,2006,157:93-98.DOI:10.1016/j.resmic.2005.10.002
[17] Marchesini MI,Morrone Seijo SM,Guaimas FF,et al.A T4SS effector targets host cell alpha-enolase contributing to Brucella abortus intracellular lifestyle[J].Front Cell Infect Microbiol,2016,16(6):153.DOI:10.3389/fcimb.2016.00153
[18] Ieira R,Comerci DJ,Sánchez DO,et al.A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication[J].J Bacteriol,2000,182(17):4849-55.DOI:10.1128/JB.182.17.4849-4855.2000
[19] Liu N,Sun C,Cui G,et al.The Rab1 in host cells modulates Brucella intracellular survival and binds to Brucella DnaK protein[J].Arch Microbiol,2016,198(9):923-31.DOI:10.1007/s00203-016-1246-0
[20] Altamirano-Silva P,Meza-Torres J,Castillo-Zeledón A,et al.Brucella abortus senses the intracellular environment through the two-component system BvrR/BvrS allowing the adaptation to its replicative niche[J].Infect Immun,2018,86(4):e00713-17.DOI:10.1128/iai.00713-17
[21] Li Z,Fu Q,Wang Z,et al.TceSR two-component regulatory system of Brucella melitensis 16M is involved in invasion,intracellular survival and regulated cytotoxicity for macrophages[J].Lett Appl Microbiol,2015,60(6):565-571.DOI:10.1111/lam.12408
[22] Kim WK,Moon JY,Kim S,et al.Comparison between immunization routes of live attenuated Salmonella Typhimurium strains expressing BCSP31,Omp3b,and SOD of Brucella abortus in murine model[J].Front Microbiol,2016,7:550.DOI:10.3389/fmicb.2016.00550
[23] Liu X,Zhou M,Yang Y,et al.Overexpression of Cu-Zn SOD in Brucella abortus suppresses bacterial intracellular replication via down-regulation of Sar1 activity[J].Oncotarget,2018,9 (11):9596-9607.DOI:10.18632/oncotarget.24073
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[31] Huy TX,Reyes AW,Hop HT,et al.Intracellular trafficking modulation by ginsenoside Rg3 inhibits Brucella abortus uptake and intracellular survival within RAW 264.7 Cells[J].J Microbiol Biotechnol,2017,27(3):616-623.DOI:10.4014/jmb.1609.09060
[32] Czyz DM,Willett J,Crosson S.Brucella abortus induces a Warburg shift in host metabolism that is linked to enhanced intracellular survival of the pathogen[J].J Bacteriol,2017,199(15):JB.00227-17.DOI:10.1128/JB.00227-17
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