RDS大鼠KLF2转录因子和甲状腺素水平相关性研究
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摘要
目的探讨KLF2转录因子在RDS大鼠肺组织的表达以及与甲状腺素水平的关系,为肺泡表面活性物质治疗不敏感的NRDS提供可能的理论依据。方法注射油酸建立的RDS大鼠模型为研究组,分别于注射后3 h、18 h、24 h用电化学发光法检测血清甲状腺素水平,用Western blot法检测肺组织中KLF2蛋白的表达,并与非RDS大鼠做对照。结果油酸3 h组、18 h组中的血清FT4与正常对照组比较差异有统计学意义(P<0. 01);油酸3 h组中T4与正常对照组比较差异有统计学意义(P<0. 01);油酸18 h组中T4与正常对照组比较差异有统计学意义(P<0. 05)。肺组织中的KLF2蛋白量表达油酸3 h组与正常对照组比较差异有统计学意义(P<0. 05),而18 h组、24 h组与正常对照组差异无统计学意义(P>0. 05)。结论呼吸窘迫综合症与血清甲状腺素水平、KLF2蛋白的表达有相关性,FT4、T4与KLF2有相关性。
Objective To investigate the expression of KLF2 transcription factor in lung tissue of RDS rats and its relationship with thyroxine level. Methods The RDS rats models( study group) induced by injecting acid were detected the serum level of thyroxine by Electrochemiluminescence after injection 3 h,18 h,24 h,and the expression of KLF2 protein in lung tissue was tested by Western blot,non-RDS rats were as contral group. Results The serum levels of FT4,T4 were significant differences between 3 h,18 h study groups and normal groups( P<0. 01,P<0. 05). The expression of KLF2 protein in lung tissue was significantly different between the 3 h study group and control group,and there were no significant differences between the 18 h,24 h study groups and control groups. Conclusion Respiratory distress syndrome is related to the serum levels of thyroxine and KLF2 protein,and FT4,T4 is related to KLF2.
Objective To investigate the expression of KLF2 transcription factor in lung tissue of RDS rats and its relationship with thyroxine level. Methods The RDS rats models( study group) induced by injecting acid were detected the serum level of thyroxine by Electrochemiluminescence after injection 3 h,18 h,24 h,and the expression of KLF2 protein in lung tissue was tested by Western blot,non-RDS rats were as contral group. Results The serum levels of FT4,T4 were significant differences between 3 h,18 h study groups and normal groups( P<0. 01,P<0. 05). The expression of KLF2 protein in lung tissue was significantly different between the 3 h study group and control group,and there were no significant differences between the 18 h,24 h study groups and control groups. Conclusion Respiratory distress syndrome is related to the serum levels of thyroxine and KLF2 protein,and FT4,T4 is related to KLF2.
引文
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[13]张亚敬,陈彦香.新生儿呼吸窘迫综合征患儿甲状腺激素、IL-6水平的变化及意义[J].宁夏医学杂志,2013,35(7):577-579.
[14]邓为彬,黄莺,曹敏,等.新生儿呼吸窘迫综合征、感染性肺炎患儿甲状腺激素水平的变化及其与IL-6、TNF-α的相关性分析[J].中国当代医药,2016,23(11):81-83.
[15]石丹丹,朱黎明. KLF2的研究进展[J].国际呼吸杂志,2013,33(14):1074-1077.
[16] Nie FQ,Sun M,Yang JS,et al. Long noncoding RNA anril promotes non-small cell lung cancer cell proliferation and inhibits apoptosis by silencing KLF2 and P21 expression[J]. Mol Cancer Ther,2015,14(1):268-277.
[17]周熙惠,惠智艳,李媛,等.新生儿呼吸窘迫综合征ABCA 3基因遗传缺陷的研究[J],中国实用儿科杂志,2012,50(2):111-116.
[2] Dobbs LG,Johnson MD,Vanderbilt J,et al. The great big alveolar TI cell:evolving concepts and paradigms[J]. Cell Physiol Biochem,2010,25(1):55-62.
[3] Herzog EL,Brody AR,Colby TV,et al. Knowns and unknowns of the alveolus[J]. Proc Am Thorac Soc,2008,5:778-782.
[4]黄烈华,吴慧莲.新生儿呼吸窘迫综合征、感染性肺炎患儿甲状腺激素、IL-6、TNF-α的变化及其相关性分析[J].中国妇幼保健,2015,3(5):716-718.
[5]张李霞,戴怡蘅.窒息新生儿甲状腺激素水平的变化及其临床意义[J].中国新生儿科杂志,2011,26(3):406-408.
[6] Ghirri P,Lunardi S,Boldrini A. Lodine supplementation in the newborn[J]. Nutrients,2014,6(1):382-390.
[7] Ibrahim M,Sinn J,Meguire W. Iodine supplementation for the prevention of mortality and adverse neurodevelopmental outcomes in preterm infants[J/CD]. Cochrane Database Syst Rev, 2006(2):CD005253.
[8] Kratzsch J,Pulzer F. Thyroid gland development and defects[J].Best Pract Res Clin Endocrinol Metab,2008,22(1):55-75.
[9] Chan SY,Franklyn JA,Pemberton HN, et al. Monocarboxylate transporter 8 expression in the human placenta:the effects of severe intrauterine growth restriction[J]. J Endocrinol, 2006, 189(3):465-471.
[10]罗颖,陈贻骥,吴季俭.早产儿暂时性低甲状腺素血症干预价值的分析[J].儿科药学杂志,2005,11(4):64.
[11] Castranova V,Jones GS,Jones GS,et al. Transport properties of isolated typeⅡalveolar epithelial cells[J]. Am Rev Respir Dis,1983,127(5 Pt 2):S28-S32.
[12] Matalon S. Mechanisms and regulation of ion transport in adult mammalian alveolar typeⅡpneumocytes[J]. Am J Physiol, 1991,261(5 Pt 1):C727-C738.
[13]张亚敬,陈彦香.新生儿呼吸窘迫综合征患儿甲状腺激素、IL-6水平的变化及意义[J].宁夏医学杂志,2013,35(7):577-579.
[14]邓为彬,黄莺,曹敏,等.新生儿呼吸窘迫综合征、感染性肺炎患儿甲状腺激素水平的变化及其与IL-6、TNF-α的相关性分析[J].中国当代医药,2016,23(11):81-83.
[15]石丹丹,朱黎明. KLF2的研究进展[J].国际呼吸杂志,2013,33(14):1074-1077.
[16] Nie FQ,Sun M,Yang JS,et al. Long noncoding RNA anril promotes non-small cell lung cancer cell proliferation and inhibits apoptosis by silencing KLF2 and P21 expression[J]. Mol Cancer Ther,2015,14(1):268-277.
[17]周熙惠,惠智艳,李媛,等.新生儿呼吸窘迫综合征ABCA 3基因遗传缺陷的研究[J],中国实用儿科杂志,2012,50(2):111-116.
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