肾蕨水提液对小鼠急性酒精肝损伤的影响
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摘要
目的:探究肾蕨水提液对小鼠急性酒精肝损伤的影响。方法:将50只健康小鼠随机分为5组:空白组、模型组、联苯双酯滴丸组(0.10 g/kg)、肾蕨水提液低剂量组(3.25 g/kg)、肾蕨水提液高剂量组(6.50 g/kg)。采用56度白酒(15 ml/kg)一次性灌胃制备小鼠急性酒精肝损伤模型。计算各组小鼠肝指数,检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)含量、谷胱甘肽(GSH)水平以及过氧化物歧化酶(SOD)活性,观察肝组织HE染色后病理变化。结果:与空白组比较,模型组肝指数、ALT、AST、MDA水平明显增高(P<0.01),SOD活性及GSH含量明显降低(P<0.05),肝细胞组织结构有显著的病理变化,肝组织结构弥漫性水肿,有大量炎症细胞浸润,肝细胞溶解坏死明显;与模型组比较,肾蕨水提液低、高剂量组均能明显降低肝指数、ALT、AST和MDA含量(P<0.01),肝匀浆中SOD活性、GSH水平明显升高(P<0.05或P<0.01);病理结果显示,肾蕨水提液低、高剂量组肝组织结构较模型组有明显改善,肝细胞坏死数量减少,有肝细胞再生表现。结论:肾蕨水提液对小鼠急性酒精肝损伤有保护作用,其机制可能与抑制肝脏炎症反应、抗脂质过氧化、清除超氧阴离子自由基有关。
Objective:To explore the effect of water extract of Nephrolepi scordifolia(L.)Presl on mice with acute alcoholic liver injury. Method:50 mice were randomly divided into 5 groups as blank control group,model group,Bifendate pills group(0.1 g/kg),Nephrolepi scordifolia(L.)Presl low dosage group(3.25 g/kg)and high dosage group(6.50 g/kg). A model of acute alcoholic liver injury in mice was prepared by intragastric administration with 56 degree alcohol(0.15 ml/10 g). By calculating the liver index,the serum liver function alanine aminotransferase and glutamicoxaloacetic transaminase(ALT and AST),the content of malondialdehyde(MDA),the level of glutathione(GSH)and the activity of superoxide dismutase(SOD)in the liver tissues were detected. The pathological changes of liver tissue after HE staining were observed. To explore the effect of water extract of Nephrolepi scordifolia(L.)Presl on mice with acute alcoholic liver injury. Results:compared with the blank control group,the liver index,ALT,AST,MDA level of the model group were significantly increased(P<0.01),while SOD,GSH decreased(P<0.05),the cellular tissue structure of liver showed significant pathological changes,liver tissue diffuse edema,a lot of inflammatory cell infiltration occurred,hepatocyte lysis and necrosis were obvious. Compared with the model group,the liver index,ALT,AST activity and MDA content decreased significantly(P <0.01)in the Nephrolepi scordifolia(L.)Presl low dosage group and high dosage group,and the the SOD activity of liver homogenate and GSH level increased(P<0.01 or P <0.05). It shows that the improved liver tissue structure,reduced number of liver cell necrosis and regeneration of liver cells in the low and high Dose group of water extract of Nephrolepi scordifolia(L.)Presl.Conclusion:The water extract of Nephrolepi scordifolia(L.)Presl shows protective effect on acute alcoholic liver injury in mice,and its mechanism may be related to anti lipid peroxidation,scavenging superoxide anion radical and inhibition of liver inflammation.
Objective:To explore the effect of water extract of Nephrolepi scordifolia(L.)Presl on mice with acute alcoholic liver injury. Method:50 mice were randomly divided into 5 groups as blank control group,model group,Bifendate pills group(0.1 g/kg),Nephrolepi scordifolia(L.)Presl low dosage group(3.25 g/kg)and high dosage group(6.50 g/kg). A model of acute alcoholic liver injury in mice was prepared by intragastric administration with 56 degree alcohol(0.15 ml/10 g). By calculating the liver index,the serum liver function alanine aminotransferase and glutamicoxaloacetic transaminase(ALT and AST),the content of malondialdehyde(MDA),the level of glutathione(GSH)and the activity of superoxide dismutase(SOD)in the liver tissues were detected. The pathological changes of liver tissue after HE staining were observed. To explore the effect of water extract of Nephrolepi scordifolia(L.)Presl on mice with acute alcoholic liver injury. Results:compared with the blank control group,the liver index,ALT,AST,MDA level of the model group were significantly increased(P<0.01),while SOD,GSH decreased(P<0.05),the cellular tissue structure of liver showed significant pathological changes,liver tissue diffuse edema,a lot of inflammatory cell infiltration occurred,hepatocyte lysis and necrosis were obvious. Compared with the model group,the liver index,ALT,AST activity and MDA content decreased significantly(P <0.01)in the Nephrolepi scordifolia(L.)Presl low dosage group and high dosage group,and the the SOD activity of liver homogenate and GSH level increased(P<0.01 or P <0.05). It shows that the improved liver tissue structure,reduced number of liver cell necrosis and regeneration of liver cells in the low and high Dose group of water extract of Nephrolepi scordifolia(L.)Presl.Conclusion:The water extract of Nephrolepi scordifolia(L.)Presl shows protective effect on acute alcoholic liver injury in mice,and its mechanism may be related to anti lipid peroxidation,scavenging superoxide anion radical and inhibition of liver inflammation.
引文
[1]钟鸣,韦松基.常用壮药临床手册[M].南宁:广西科学技术出版社,2010:668.
[2]梁启成,钟鸣.中国壮药学[M]南宁:广西民族出版社,2005:265.
[3]朱华,马雯芳,邓慧连,等.壮药肾蕨质量标准初步研究[J].广西中医药,2012,35(3):52-54.
[4]黄锁义,罗建华,蒙春越,等.超声波提取肾蕨总黄酮及鉴别[J].时珍国医国药,2007,18(1):154-155.
[5]康丽杰,赵涵,李妍,等.肾蕨叶总黄酮提取条件优化[J].甘肃医药,2017,36(10):878-880.
[6]谢凤凤,黎理,马雯芳,等.肾蕨多糖提取工艺优化及其含量测定研究[J].临床医药文献杂志,2017,4(41):7933-7935.
[7]陈晓清,苏育才,李晓晶,等.抗菌肾蕨多糖的提取与分离[J].漳州师范学院学报:自然科学版,2006,19(4):112-115.
[8]龙友国,余跃生,戎聚全,等.肾蕨抗菌和抗衰老作用的实验研究[J].黔南民族医专学报,2007,20(1):4-6.
[9]王音音.红豆杉(Taxus chinensis)植物替代资源的调查研究[D].广州:华南农业大学,2007.
[10]闫循齐.肾蕨查尔酮合成酶基因的克隆、表达及其影响因素[D].上海:上海师范大学,2016.
[11]程学敏,黎海娟,黄茜,等.绿茶多酚对急性酒精性肝损伤小鼠抗氧化能力及细胞色素C水平的影响[J].中国老年学杂志,2017,37(1):40-42.
[12]姚秀芬,程栋,王承明.花生粗多糖对四氯化碳及酒精所致小鼠急性肝损伤的保护作用[J].食品科学,2011,32(9):261-265.
[13]黄庆科,林菊生,陈必成.血红素加氧酶1对大鼠急性酒精性肝损害保护作用的实验研究[J].中华消化杂志,2007,27(7):490-491.
[14]韦日明,高雅,张可锋,等.杠板归总黄酮对小鼠酒精性肝损伤的保护作用[J].南方农业学报,2016,47(4):664-669.
[15]杨牧祥,姚树坤.解酒护肝饮对酒精性肝损伤大鼠血清和肝组织ALT,AST的影响[J].河北中医,2000,22(10):793-796.
[16]吴道勋,张娜,邵维莉,等.云南松松塔提取物对小鼠急性酒精性肝损伤的影响[J].中国中医药信息杂志,2017,24(8):46-49.
[2]梁启成,钟鸣.中国壮药学[M]南宁:广西民族出版社,2005:265.
[3]朱华,马雯芳,邓慧连,等.壮药肾蕨质量标准初步研究[J].广西中医药,2012,35(3):52-54.
[4]黄锁义,罗建华,蒙春越,等.超声波提取肾蕨总黄酮及鉴别[J].时珍国医国药,2007,18(1):154-155.
[5]康丽杰,赵涵,李妍,等.肾蕨叶总黄酮提取条件优化[J].甘肃医药,2017,36(10):878-880.
[6]谢凤凤,黎理,马雯芳,等.肾蕨多糖提取工艺优化及其含量测定研究[J].临床医药文献杂志,2017,4(41):7933-7935.
[7]陈晓清,苏育才,李晓晶,等.抗菌肾蕨多糖的提取与分离[J].漳州师范学院学报:自然科学版,2006,19(4):112-115.
[8]龙友国,余跃生,戎聚全,等.肾蕨抗菌和抗衰老作用的实验研究[J].黔南民族医专学报,2007,20(1):4-6.
[9]王音音.红豆杉(Taxus chinensis)植物替代资源的调查研究[D].广州:华南农业大学,2007.
[10]闫循齐.肾蕨查尔酮合成酶基因的克隆、表达及其影响因素[D].上海:上海师范大学,2016.
[11]程学敏,黎海娟,黄茜,等.绿茶多酚对急性酒精性肝损伤小鼠抗氧化能力及细胞色素C水平的影响[J].中国老年学杂志,2017,37(1):40-42.
[12]姚秀芬,程栋,王承明.花生粗多糖对四氯化碳及酒精所致小鼠急性肝损伤的保护作用[J].食品科学,2011,32(9):261-265.
[13]黄庆科,林菊生,陈必成.血红素加氧酶1对大鼠急性酒精性肝损害保护作用的实验研究[J].中华消化杂志,2007,27(7):490-491.
[14]韦日明,高雅,张可锋,等.杠板归总黄酮对小鼠酒精性肝损伤的保护作用[J].南方农业学报,2016,47(4):664-669.
[15]杨牧祥,姚树坤.解酒护肝饮对酒精性肝损伤大鼠血清和肝组织ALT,AST的影响[J].河北中医,2000,22(10):793-796.
[16]吴道勋,张娜,邵维莉,等.云南松松塔提取物对小鼠急性酒精性肝损伤的影响[J].中国中医药信息杂志,2017,24(8):46-49.