山东省某地区牛、羊肠道病毒感染的流行病学调查
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摘要
牛、羊肠道病毒感染为国内新近报道的动物传染病,其流行病学本底在国内缺乏。为了解牛、羊肠道病毒感染的情况,本研究应用双抗体夹心ELISA方法对采集于山东省某地区的奶牛、肉牛、绵羊和山羊粪便样品进行了肠道病毒的检测。同时对ELISA检测为阳性的部分样品进行了病毒分离,并以免疫过氧化物酶单层试验对分离毒株进行了鉴定。结果发现,奶牛的肠道病毒感染率为25.0%,肉牛群的感染率为7.6%,绵羊的肠道病毒感染率58.6%,山羊的肠道病毒感染率为48.5%,该结果为肠道病毒感染的防制提供流行病学的理论依据。
Enterovirus infections in cattle and sheep/goat are emerging animal infectious diseases in our country,and their epidemiological backgrounds remain largely unknown.To understand the potential enterovirus infection in ruminants(cattle/sheep/goat),a double-antibody sandwich ELISA method was employed to detect enterovirus antigen in specimens collected from dairy cows,feedlot cattle,sheep,and goat herds in Shandong Province.The results showed that BEV infection rates in dairy cows and feedlot cattle were 25.0%and 7.6%,respectively.The positive rates for enterovirus infection in sheep and goat were 58.6%and 48.5%,respectively.These findings provided epidemiological basis for the prevention and control of enterovirus infections.
Enterovirus infections in cattle and sheep/goat are emerging animal infectious diseases in our country,and their epidemiological backgrounds remain largely unknown.To understand the potential enterovirus infection in ruminants(cattle/sheep/goat),a double-antibody sandwich ELISA method was employed to detect enterovirus antigen in specimens collected from dairy cows,feedlot cattle,sheep,and goat herds in Shandong Province.The results showed that BEV infection rates in dairy cows and feedlot cattle were 25.0%and 7.6%,respectively.The positive rates for enterovirus infection in sheep and goat were 58.6%and 48.5%,respectively.These findings provided epidemiological basis for the prevention and control of enterovirus infections.
引文
[1]邢泽黎,朱利塞,王新平,等.从吉林省肉牛场新发疫病分离出E种肠道病毒[J].中国兽医学报,2014,34(3):390-394.
[2]ZHU L,XING Z,GAI X,et al.Identification of a novel enterovirus E isolates HY12from cattle with severe respiratory and enteric diseases[J].PLoS One,2014,9(5):e97730.
[3]王明月,王新平,朱利塞,等.从暴发严重腹泻羊群中检出G种肠道病毒[J].中国兽医学报,2016,36(10):1692-1695.
[4]WANG M,JIA H,LU H,et al.A novel enterovirus species identified from severe diarrheal goats[J].PLoSOne,2017,12(4):e0174600.
[5]李英利,常继涛,于力.牛肠道病毒的分离鉴定[J].中国预防兽医学报,2011,33(5):388-390.
[6]彭小薇,董浩,吴丹,等.牛肠道病毒2型的分离与鉴定[J].畜牧兽医学报,2013,44(5):823-828.
[7]张海丽,周萍萍,高明春,等.一株牛肠道病毒的分离与鉴定[J].中国奶牛,2015(5):31-34.
[8]ADAMS M J,LEKKOWITZ E J,KING A M Q,et al.Changes to taxonomy and the international code of virus classification and nomenclature ratified by the international committee on taxonomy of viruses(2017)[J].Arch Virol,2017,162(8):2505-2538.
[9]ZELL R.Picornaviridae-the ever-growing virus family[J].Arch Virol,2018,163(2):299-317.
[10]BOROS A,PANKOVICS P,KNOWLES N J,et al.Natural interspecies recombinant bovine/porcine enterovirus in sheep[J].J Gen Virol,2012,93(9):1941-1951.
[11]OMATSU T,TSUCHIAKA S,HIRATA T,et al.Detection of enterovirus genome sequence from diarrheal feces of goat[J].Virus Gen,2014,48(3):550-552.
[12]MOLL T,DAVIS A D.Isolation and characterization of cytopathogenic enteroviruses from cattle with respiratory disease[J].Am J Vet Res,1959,20(8):27.
[13]DUNNE H W,HUANG C M,LIN W J.Bovine enteroviruses in the calf:an attempt at serologic,biologic,and pathologic classification[J].J Am Vet Med A,1974,164(3):290-294.
[14]CARTHEW P,MARTIN S J.The iodination of bovine enterovirus particles[J].J Gen Virol,1974,24(3):525-534.
[15]ACAR A,GUR S.Seroprevalance of bovine enterovirus type 1(BEV1)in goats in Turkey[J].J Anim Vet Advan,2012,8(6):1075-1078.
[16]MCCLEANAHAN S D,SCHERBA G,BORST L,et al.Discovery of a bovine enterovirus in Alpaca[J].PLoS One,2013,8(8):e68777.
[17]GOENS S D,BOTERO S,ZEMILA A,et al.Bovine enterovirus 2:complete genomic sequence and molecular modelling of a reference strain and a wild-type isolate from endemically infected US cattle[J].J Gen Virol,2004,85(11):3195-3203.
[18]邢泽黎,王新平,朱利塞,等.双抗体夹心ELISA检测牛肠道病毒抗原方法的建立及流行病学调查[J].中国兽医学报,2016,36(4):567-571.
[19]BUNKE J,RECEVEUR K,OESER A C,et al.High genetic diversity of porcine enterovirus G in Schleswig-Holstein,Germany[J].Arch Virol,2017,163(9):1-5.
[20]鲁海冰,胡俊英,张群,等.双抗体夹心ELISA检测羊肠道病毒抗原方法的建立及病原流行病学调查[J].中国兽医学报,2017,37(4):653-657.
[21]侯佩莉,王洪梅,何洪彬.牛病毒性腹泻病毒、牛冠状病毒和牛肠道病毒多重RT-PCR检测方法的建立及初步应用[J].中国兽医学报,2016,36(10):1672-1675,1700.
[22]刘亚静,李欣,张群,等.吉林省不同地区牛肠道病毒遗传变异分析[J].中国兽医学报,2018,38(1):59-63.
[23]张群,鲁海冰,郭昌明,等.羊肠道病毒与小反刍兽疫病毒混合感染及流行病学调查[J].中国兽医学报,2018,38(3):464-468.
[2]ZHU L,XING Z,GAI X,et al.Identification of a novel enterovirus E isolates HY12from cattle with severe respiratory and enteric diseases[J].PLoS One,2014,9(5):e97730.
[3]王明月,王新平,朱利塞,等.从暴发严重腹泻羊群中检出G种肠道病毒[J].中国兽医学报,2016,36(10):1692-1695.
[4]WANG M,JIA H,LU H,et al.A novel enterovirus species identified from severe diarrheal goats[J].PLoSOne,2017,12(4):e0174600.
[5]李英利,常继涛,于力.牛肠道病毒的分离鉴定[J].中国预防兽医学报,2011,33(5):388-390.
[6]彭小薇,董浩,吴丹,等.牛肠道病毒2型的分离与鉴定[J].畜牧兽医学报,2013,44(5):823-828.
[7]张海丽,周萍萍,高明春,等.一株牛肠道病毒的分离与鉴定[J].中国奶牛,2015(5):31-34.
[8]ADAMS M J,LEKKOWITZ E J,KING A M Q,et al.Changes to taxonomy and the international code of virus classification and nomenclature ratified by the international committee on taxonomy of viruses(2017)[J].Arch Virol,2017,162(8):2505-2538.
[9]ZELL R.Picornaviridae-the ever-growing virus family[J].Arch Virol,2018,163(2):299-317.
[10]BOROS A,PANKOVICS P,KNOWLES N J,et al.Natural interspecies recombinant bovine/porcine enterovirus in sheep[J].J Gen Virol,2012,93(9):1941-1951.
[11]OMATSU T,TSUCHIAKA S,HIRATA T,et al.Detection of enterovirus genome sequence from diarrheal feces of goat[J].Virus Gen,2014,48(3):550-552.
[12]MOLL T,DAVIS A D.Isolation and characterization of cytopathogenic enteroviruses from cattle with respiratory disease[J].Am J Vet Res,1959,20(8):27.
[13]DUNNE H W,HUANG C M,LIN W J.Bovine enteroviruses in the calf:an attempt at serologic,biologic,and pathologic classification[J].J Am Vet Med A,1974,164(3):290-294.
[14]CARTHEW P,MARTIN S J.The iodination of bovine enterovirus particles[J].J Gen Virol,1974,24(3):525-534.
[15]ACAR A,GUR S.Seroprevalance of bovine enterovirus type 1(BEV1)in goats in Turkey[J].J Anim Vet Advan,2012,8(6):1075-1078.
[16]MCCLEANAHAN S D,SCHERBA G,BORST L,et al.Discovery of a bovine enterovirus in Alpaca[J].PLoS One,2013,8(8):e68777.
[17]GOENS S D,BOTERO S,ZEMILA A,et al.Bovine enterovirus 2:complete genomic sequence and molecular modelling of a reference strain and a wild-type isolate from endemically infected US cattle[J].J Gen Virol,2004,85(11):3195-3203.
[18]邢泽黎,王新平,朱利塞,等.双抗体夹心ELISA检测牛肠道病毒抗原方法的建立及流行病学调查[J].中国兽医学报,2016,36(4):567-571.
[19]BUNKE J,RECEVEUR K,OESER A C,et al.High genetic diversity of porcine enterovirus G in Schleswig-Holstein,Germany[J].Arch Virol,2017,163(9):1-5.
[20]鲁海冰,胡俊英,张群,等.双抗体夹心ELISA检测羊肠道病毒抗原方法的建立及病原流行病学调查[J].中国兽医学报,2017,37(4):653-657.
[21]侯佩莉,王洪梅,何洪彬.牛病毒性腹泻病毒、牛冠状病毒和牛肠道病毒多重RT-PCR检测方法的建立及初步应用[J].中国兽医学报,2016,36(10):1672-1675,1700.
[22]刘亚静,李欣,张群,等.吉林省不同地区牛肠道病毒遗传变异分析[J].中国兽医学报,2018,38(1):59-63.
[23]张群,鲁海冰,郭昌明,等.羊肠道病毒与小反刍兽疫病毒混合感染及流行病学调查[J].中国兽医学报,2018,38(3):464-468.