磁性纳米分子印迹聚合物的制备及其对生物体内PQQ-DA痕量产物的研究
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摘要
以磁性Fe_3O_4为内核,对磁性纳米粒子表面进行包裹和修饰,制备了对吡咯并喹啉醌-多巴胺(PQQ-DA)具有高识别性、高选择性的磁性纳米分子印迹材料,并结合超高效液相色谱-质谱(UPLC-MS)测定了生物体内PQQ-DA的含量。结果显示,PQQ-DA在0.02～0.1 mg/mL范围内呈良好的线性关系,相关系数(r~2)为0.996 6,检出限为0.2×10~(-11) mg/mL,加标回收率为88.7%～95.5%,相对标准偏差为2.6%～3.2%。该方法灵敏度高、重复性好,可用于生物体内PQQ-DA痕量产物的检测。
A pyrroloquinoquinone(PQQ)-dopamine(DA)magnetic nanoparticle imprinted polymers were prepared by the combination of PQQ-DA and magnetic nano-Fe_3O_4 as the core wrapped with SiO_2,which possess high recognition and high selectivity toward PQQ-DA and have great advantages in the separation,purification and enrichment of low concentration compounds in complex matrix of brain tissue after molecular imprinting modification.The magnetic nanoparticles were applied in the determination of trace PQQ-DA in brain tissue samples by ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS).Results showed that,under the optimized conditions,there was a good linear relationship for PQQ-DA in the range of 0.02-0.1 mg/mL with a correlation coefficient(r~2)of 0.996 6,and the detection limit was 0.2×10~(-11) mg/mL.The recoveries for brain tissue samples were in the range of 88.7%-95.5%,with relative standard deviations(n=6)of 2.6%-3.2%.The method could be used in the detection of trace PQQ-DA in vivo with high sensitivity and good repeatability.
A pyrroloquinoquinone(PQQ)-dopamine(DA)magnetic nanoparticle imprinted polymers were prepared by the combination of PQQ-DA and magnetic nano-Fe_3O_4 as the core wrapped with SiO_2,which possess high recognition and high selectivity toward PQQ-DA and have great advantages in the separation,purification and enrichment of low concentration compounds in complex matrix of brain tissue after molecular imprinting modification.The magnetic nanoparticles were applied in the determination of trace PQQ-DA in brain tissue samples by ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS).Results showed that,under the optimized conditions,there was a good linear relationship for PQQ-DA in the range of 0.02-0.1 mg/mL with a correlation coefficient(r~2)of 0.996 6,and the detection limit was 0.2×10~(-11) mg/mL.The recoveries for brain tissue samples were in the range of 88.7%-95.5%,with relative standard deviations(n=6)of 2.6%-3.2%.The method could be used in the detection of trace PQQ-DA in vivo with high sensitivity and good repeatability.
引文
[1]Segura-Aguilar J,Paris I,Mu1oz P,Ferrari E,Zecca L,Zucca F A.J.Neurochem.,2014,129(6):898-915.
[2]Pahuja R,Seth K,Shukla A,Shukla R K,Priyanka B P,Chauhan L K S,Saxena P N,Arun J,Chaudhari B P,Patel D K,Singh S P,Shukla R,Khanna V K,Kumar P,Chaturvedi R K,Gupta K C.ACS Nano,2015,9(5):4850-4871.
[3]Stites T,Storms D,Bauerly K,Mah J,Harris C,Fascetti A,Rogers Q,Tchaparian E,Satre M,Rucker R B.J.Nutr.,2006,136:390-396.
[4]Tang L,Li L Z,Hu W M,Hu Z H,Zhang L,Zhang L J,Mei S,Chen J G,Liu D Y,Liu Z,Wang Y.Chin.J.Food Hyg.(唐靓,李林子,胡文敏,胡志航,张岭,张丽婧,梅松,陈建国,刘冬英,刘臻,王茵.中国食品卫生杂志),2017,29(4):407-411.
[5]Zhou X Q,Qin X F,Zhang J K,Cao G X.Chin.J.Chromatogr.(周杏琴,钦晓峰,张建康,曹国宪.色谱),2012,30(2):196-200.
[6]Shen C F,Zhang Z F,Guo Y A,Yan G Q.J.Instrum.Anal.(申晨凡,张直峰,郭瑜桉,闫桂琴.分析测试学报),2016,35(08):949-954.
[7]Lin L H,Zhou Q,Bu S L.J.Instrum.Anal.(林立华,周群,卜胜利.分析测试学报),2016,35(11):1481-1485.
[8]Liao X T,Liao M M.J.Instrum.Anal.(廖晓棠,廖明媚.分析测试学报),2018,37(04):487-491.
[9]Guo T X,Lou D W,Lian L L,Wang X Y,Chen H J,Li Q Y,Wang R.J.Instrum.Anal.(郭亭秀,娄大伟,连丽丽,王希越,陈慧君,李秋颖,王润.分析测试学报),2016,35(9):1137-1141.
[10]Fouad D M,El-Said W A,Mohamed M B.Spectrochim.Acta A,2015,140:392-397.
[11]Kang J R,Mao X,Wu X H,Yang H.Chin.J.Anal.Lab.(康静茹,毛翔,吴星杭,杨海.分析试验室),2018,9(37):1102-1109.
[12]Zhu G Q,He Q K,Liu C G,Xiao S M,Zeng Y.J.Nat.Sci.Hunan Norm.Univ.(朱广谦,何青科,刘长庚,肖尚马,曾盈.湖南师范大学自然科学学报),2016,39(3):46-55,74.
[13]Mao S S,Qin X F,Zhou X Q.Chin.J.Anal.Lab.(毛师师,钦晓峰,周杏琴.分析试验室),2015,34(8):874-877.
[14]Yin X Y,Zhong Y Q,Jiang Y F,Luo Y M.Spectrosc.Spect.Anal.(尹小英,衷友泉,江一帆,罗永明.光谱学与光谱分析),2010,30(8):2211-2214.
[15]Ren H P,Guan Y Y,Dai R H,Liu G Y,Chai C Y.Spectrosc.Spect.Anal.(任慧鹏,管毓渝,戴荣华,刘国艳,柴春彦.光谱学与光谱分析),2016,36(2):372-378.
[16]Yang T,Chen G,Wu Y L,Zhao J,Zhu Y.Acta Agr.Zhejiangensis(杨挺,陈国,吴银良,赵健,朱勇.浙江农业学报),2012,24(5):792-796.
[17]Chen J B,Sun H F,You J Q,Zhu G T,He J,Xie T.J.Instrum.Anal.(陈嘉彬,孙海峰,游金清,朱钢添,何君,谢涛.分析测试学报),2018,37(5):588-593.
[2]Pahuja R,Seth K,Shukla A,Shukla R K,Priyanka B P,Chauhan L K S,Saxena P N,Arun J,Chaudhari B P,Patel D K,Singh S P,Shukla R,Khanna V K,Kumar P,Chaturvedi R K,Gupta K C.ACS Nano,2015,9(5):4850-4871.
[3]Stites T,Storms D,Bauerly K,Mah J,Harris C,Fascetti A,Rogers Q,Tchaparian E,Satre M,Rucker R B.J.Nutr.,2006,136:390-396.
[4]Tang L,Li L Z,Hu W M,Hu Z H,Zhang L,Zhang L J,Mei S,Chen J G,Liu D Y,Liu Z,Wang Y.Chin.J.Food Hyg.(唐靓,李林子,胡文敏,胡志航,张岭,张丽婧,梅松,陈建国,刘冬英,刘臻,王茵.中国食品卫生杂志),2017,29(4):407-411.
[5]Zhou X Q,Qin X F,Zhang J K,Cao G X.Chin.J.Chromatogr.(周杏琴,钦晓峰,张建康,曹国宪.色谱),2012,30(2):196-200.
[6]Shen C F,Zhang Z F,Guo Y A,Yan G Q.J.Instrum.Anal.(申晨凡,张直峰,郭瑜桉,闫桂琴.分析测试学报),2016,35(08):949-954.
[7]Lin L H,Zhou Q,Bu S L.J.Instrum.Anal.(林立华,周群,卜胜利.分析测试学报),2016,35(11):1481-1485.
[8]Liao X T,Liao M M.J.Instrum.Anal.(廖晓棠,廖明媚.分析测试学报),2018,37(04):487-491.
[9]Guo T X,Lou D W,Lian L L,Wang X Y,Chen H J,Li Q Y,Wang R.J.Instrum.Anal.(郭亭秀,娄大伟,连丽丽,王希越,陈慧君,李秋颖,王润.分析测试学报),2016,35(9):1137-1141.
[10]Fouad D M,El-Said W A,Mohamed M B.Spectrochim.Acta A,2015,140:392-397.
[11]Kang J R,Mao X,Wu X H,Yang H.Chin.J.Anal.Lab.(康静茹,毛翔,吴星杭,杨海.分析试验室),2018,9(37):1102-1109.
[12]Zhu G Q,He Q K,Liu C G,Xiao S M,Zeng Y.J.Nat.Sci.Hunan Norm.Univ.(朱广谦,何青科,刘长庚,肖尚马,曾盈.湖南师范大学自然科学学报),2016,39(3):46-55,74.
[13]Mao S S,Qin X F,Zhou X Q.Chin.J.Anal.Lab.(毛师师,钦晓峰,周杏琴.分析试验室),2015,34(8):874-877.
[14]Yin X Y,Zhong Y Q,Jiang Y F,Luo Y M.Spectrosc.Spect.Anal.(尹小英,衷友泉,江一帆,罗永明.光谱学与光谱分析),2010,30(8):2211-2214.
[15]Ren H P,Guan Y Y,Dai R H,Liu G Y,Chai C Y.Spectrosc.Spect.Anal.(任慧鹏,管毓渝,戴荣华,刘国艳,柴春彦.光谱学与光谱分析),2016,36(2):372-378.
[16]Yang T,Chen G,Wu Y L,Zhao J,Zhu Y.Acta Agr.Zhejiangensis(杨挺,陈国,吴银良,赵健,朱勇.浙江农业学报),2012,24(5):792-796.
[17]Chen J B,Sun H F,You J Q,Zhu G T,He J,Xie T.J.Instrum.Anal.(陈嘉彬,孙海峰,游金清,朱钢添,何君,谢涛.分析测试学报),2018,37(5):588-593.
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