一测多评法测定甜荞中6种酚类成分
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摘要
建立了一测多评法(QAMS)测定甜荞中6种酚类成分的含量。采用超高效液相色谱法,色谱柱为ACQUITYUPLC~?BEHC18(1.7μm,2.1 mm×50 mm),甲醇-0.2%冰乙酸水溶液为流动相梯度洗脱,检测波长254 nm,流速0.2 mL/min,柱温35℃;以芦丁为内参物,建立其与儿茶素、表儿茶素、阿魏酸、槲皮苷、槲皮素的相对校正因子,采用一测多评法和外标法(ESM)同时计算各待测成分的含量,并进行比较。结果表明,10种甜荞品种中儿茶素、表儿茶素、阿魏酸、芦丁、槲皮苷、槲皮素的含量可以用一测多评法进行测定,一测多评法测定的结果与外标法无明显差异(P>0.05),并且10个品种中红花甜荞的6种酚类成分量最高,为112.94μg/g;最低是丰甜1号,含量为54.60μg/g。文章建立的一测多评法操作简单、重复性好,可为甜荞的质量评价提供参考。
A quantitative method of analysis of multi-components by single marker(QAMS) method for the quantification of six polyphenols in buckwheat was established. Samples were analyzed by Ultra Performance Liquid Chromatography(UPLC) system, equipped with a ACQUITYUPLC?BEHC18(1.7 μm,2.1 mm×50 mm) chromatographic column, with the mobile phase of methanol-0.2% glacial acetic acid solution at a flow rate of 0.2 mL/min. The column temperature and the detection wavelength set at 35 ℃and 254 nm respectively. The rutin was used as the internal reference to determine the relative correction factors(RCFs) of other five components, and their contents were calculated by their RCFs at the same time, then compared with the external standard method(ESM). No significant differences were found in the quantitative results of six polyphenols in 10 varieties of buckwheat determined by ESM and QAMS(RSD<5%). The highest total content of 6 phenolic substances in 10 varieties of buckwheat is Honghua(112.94 μg/g), The lowest is Fengtian(54.60 μg/g). It is feasible and suitable to evaluate the quality of buckwheat by QAMS.
A quantitative method of analysis of multi-components by single marker(QAMS) method for the quantification of six polyphenols in buckwheat was established. Samples were analyzed by Ultra Performance Liquid Chromatography(UPLC) system, equipped with a ACQUITYUPLC?BEHC18(1.7 μm,2.1 mm×50 mm) chromatographic column, with the mobile phase of methanol-0.2% glacial acetic acid solution at a flow rate of 0.2 mL/min. The column temperature and the detection wavelength set at 35 ℃and 254 nm respectively. The rutin was used as the internal reference to determine the relative correction factors(RCFs) of other five components, and their contents were calculated by their RCFs at the same time, then compared with the external standard method(ESM). No significant differences were found in the quantitative results of six polyphenols in 10 varieties of buckwheat determined by ESM and QAMS(RSD<5%). The highest total content of 6 phenolic substances in 10 varieties of buckwheat is Honghua(112.94 μg/g), The lowest is Fengtian(54.60 μg/g). It is feasible and suitable to evaluate the quality of buckwheat by QAMS.
引文
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[15]宋永贵,张武岗,刘岩庭,等.一测多评法同时测定预知子中4种三萜皂苷[J].中草药,2012,43(7):1418-1421.
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[17]Ali Rifat Gulpinar,Ilkay Erdogan Orhan,Asuman Kan,et al.Estimation of in vitro neuroprotective properties and quantification of rutin and fatty acids in buckwheat(Fagopyrum esculentum Moench)cultivated in Turkey[J].Food Research International,2012,46:536-543.
[18]Ahmad Ghorbani.Mechanisms of antidiabetic effects of flavonoid rutin[J].Biomedicine&Pharmacotherapy,2017,96:305-312.
[19]Aditya Ganeshpurkar,Ajay K Saluja.The Pharmacological Potential of Rutin[J].Saudi Pharmaceutical Journal,2017,25:149-164.
[20]马麟,彭镰心,曹靖,等.超声波辅助提取RP-HPLC法测定藜麦中的3种碳苷黄酮[J].食品工业,2016,37(7):192-195.
[21]魏丽娟,易倩,张曲,等.一测多评法测定藜麦中6种酚类成分[J].食品工业科技,2018,39(19):232-236,242.
[2]Laura Siracusa,Fabio Gresta,Elisa Sperlinga,et al.Effect of sowing time and soil water content on grain yield and phenolic profile of four buckwheat(Fagopyrum esculentum Moench.)varieties in a Mediterranean environment[J].Journal of Food Composition and Analysis,2017,62:1-7.
[3]Siu-Mei Choi,Ching-Yung Ma.Extraction,purification and characterization of globulin from common buckwheat(Fagopyrum esculentum Moench)seeds[J].Food Research International,2006,39:974-981.
[4]张美莉,吴继红,赵镭,等.苦荞和甜荞萌发后氨基酸含量变化及其营养评价[J].食品与发酵工业,2005,31(3):115-118.
[5]成少宁.萌发荞麦抗氧化活性及抑菌效果研究[D].上海:上海师范大学,2010:42-46.
[6]韩淑英,吕华,朱丽莎,等.荞麦种子总黄酮降血脂、血糖及抗脂质过氧化作用的研究[J].中国药理学通报,2001,17(6):694-696.
[7]Dimitrios Stagos,Gregorios D Amoutzias,Antonios Matakos,et al.Chemoprevention of liver cancer by plant polyphenols[J].Food and Chemical Toxicology,2012,50:2155-2170.
[8]Raffaella Soleti,Ramaroson Andriantsitohaina,MCarmen Martinez.Impact of polyphenols on extracellular vesicle levels and effects and their properties as tools for drug delivery for nutrition and health[J].Archives of Biochemistry and Biophysics,2018,664:57-63.
[9]Biljana Kiprovski,Maja Mikulic-Petkovsek,Ana Slatnar,et al.Comparison of phenolic profiles and antioxidant properties of European Fagopyrumes culentum cultivars[J].Food Chemistry,2015,185:41-47.
[10]LI F H,YUAN Y,YANG X L,et al.Phenolic Profiles and Antioxidant Activity of Buckwheat(Fagopyrum esculentum M?ench and Fagopyrum tartaricum L.Gaerth)Hulls,Brans and Flours[J].Journal of Integrative Agriculture,2013,12(9):1684-1693.
[11]张琪,刘慧灵,朱瑞,等.苦荞麦中总黄酮和芦丁的含量测定方法的研究[J],食品科学,2003,24(7):113-116.
[12]Vaida Kraujalien?,Audrius Pukalskas,Petras Rimantas Venskutonis.Multi-stage recovery of phytochemicals from buckwheat(Fagopyrum esculentum Moench)flowers by supercritical fluid and pressurized liquid extraction methods[J].Industrial Crops&Products,2017,107:271-280.
[13]胡鞒缤,姚瑛瑛,李艳琴,等.荞麦植株各部位总黄酮含量的测定与比较[J].食品与药品,2013,15(6):394-396.
[14]匡艳辉,朱晶晶,王智民,等.一测多评法测定黄连中小檗碱、巴马汀、黄连碱、表小檗碱、药根碱含量[J].中国药学杂志,2009,44(5):390-394.
[15]宋永贵,张武岗,刘岩庭,等.一测多评法同时测定预知子中4种三萜皂苷[J].中草药,2012,43(7):1418-1421.
[16]赵玉平,肖春玲.苦荞麦不同器官总黄酮含量测定及分析[J],食品科学,2004,25(10):264-266.
[17]Ali Rifat Gulpinar,Ilkay Erdogan Orhan,Asuman Kan,et al.Estimation of in vitro neuroprotective properties and quantification of rutin and fatty acids in buckwheat(Fagopyrum esculentum Moench)cultivated in Turkey[J].Food Research International,2012,46:536-543.
[18]Ahmad Ghorbani.Mechanisms of antidiabetic effects of flavonoid rutin[J].Biomedicine&Pharmacotherapy,2017,96:305-312.
[19]Aditya Ganeshpurkar,Ajay K Saluja.The Pharmacological Potential of Rutin[J].Saudi Pharmaceutical Journal,2017,25:149-164.
[20]马麟,彭镰心,曹靖,等.超声波辅助提取RP-HPLC法测定藜麦中的3种碳苷黄酮[J].食品工业,2016,37(7):192-195.
[21]魏丽娟,易倩,张曲,等.一测多评法测定藜麦中6种酚类成分[J].食品工业科技,2018,39(19):232-236,242.