冠突散囊菌发酵产物的抑菌作用及特性研究
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摘要
目的测定冠突散囊菌发酵液不同溶剂提取物的抑菌作用、抑菌物质的温度及其p H稳定性。方法依次采用石油醚、乙酸乙酯、正丁醇提取冠突散囊菌发酵液,采用打孔法测定冠突散囊菌发酵液不同溶剂提取物对细菌、真菌及放线菌的抑菌作用及在不同温度及p H值下的稳定性。结果发酵液乙酸乙酯提取相的抑菌作用较其他提取相强。其中,对枯草芽孢杆菌与白色链霉菌的抑制作用最显著,且该抑菌物质在25～121℃,p H 4～8范围内均能保持稳定的抑菌活性。结论乙酸乙酯能有效提取冠突散囊菌发酵液中的抑菌物质,抑菌物质在一定的温度及p H范围内具有良好的稳定性。
Objective To determine the antimicrobial effect of different solvent extracts from the fermentation broth of Eurotium cristatum, and the temperature and pH stability of the antimicrobial substances. Methods The substances was extracted with petroleum ether, ethyl acetate and n-butanol successively, and the punching method was used to determine the antimicrobial effect of different solvent extracts from the fermentation broth of Eurotium cristatum on bacteria, fungi and actinomycetes. The stability of antimicrobial substances was investigated at different temperatures and pH values. Results The antimicrobial activity of ethyl acetate phase was stronger than that of other extracts, and the inhibitive effect on Bacillus subtilis and Streptomyces albicans was signi?cant. The antimicrobial substances were stable under the condition of 25-80 ℃, pH 3-9. Conclusion Ethyl acetate can effectively extract the antimicrobial substances in the fermentation broth of Eurotium cristatum, and the antimicrobial effect has a good stability in a certain temperature and pH range.
Objective To determine the antimicrobial effect of different solvent extracts from the fermentation broth of Eurotium cristatum, and the temperature and pH stability of the antimicrobial substances. Methods The substances was extracted with petroleum ether, ethyl acetate and n-butanol successively, and the punching method was used to determine the antimicrobial effect of different solvent extracts from the fermentation broth of Eurotium cristatum on bacteria, fungi and actinomycetes. The stability of antimicrobial substances was investigated at different temperatures and pH values. Results The antimicrobial activity of ethyl acetate phase was stronger than that of other extracts, and the inhibitive effect on Bacillus subtilis and Streptomyces albicans was signi?cant. The antimicrobial substances were stable under the condition of 25-80 ℃, pH 3-9. Conclusion Ethyl acetate can effectively extract the antimicrobial substances in the fermentation broth of Eurotium cristatum, and the antimicrobial effect has a good stability in a certain temperature and pH range.
引文
[1]温琼英.茯砖茶中优势菌的种名鉴定[J].中国茶叶,1990,(6):2-3.
[2]傅冬和.高通量筛选研究茯砖茶降脂功效[J].茶叶科学,2006,26(3):209-214.
[3]傅冬和.高通量筛选研究茯砖茶对FXR模型的作用[J].食品科学, 2007, 28(5):331-334.
[4]吕嘉枥,郑欣欣,马亚宁.冠突散囊菌液体发酵产降脂类物质的初步研究[J].陕西科技大学学报(自然科学版), 2014, 32(6):115-118.
[5]蔡正安,刘素纯,刘仲华,等.茯砖茶中冠突散囊菌纤维素酶的酶学性质研究[J].茶叶科学, 2010, 30(1):57-62.
[6]李玉婷,吕嘉枥,李良凤,等.冠突散囊菌固态发酵产消化酶活力的研究[J].中国酿造, 2012, 31(10):93-95.
[7]黄群,陈林杰,李彦坡,等.冠突散囊菌黑茶发酵液对消化酶活性影响的研究[J].微生物学通报, 2007, 34(5):917-920.
[8]李莹,邹先伟,张小娜,等.冠突散囊菌不同溶剂提取物体外抗氧化活性研究[J].中国实验方剂学杂志, 2014, 20(7):143-146.
[9]欧阳梅,熊昌云,屠幼英,等.冠突散囊菌对茶叶品质成分及其抗氧化活性影响[J].菌物学报, 2011, 30(2):343-348.
[10]李佳莲,胡博涵,赵勇彪,等.冠突散囊菌发酵液的抑菌作用[J].食品科学, 2011, 32(11):157-160.
[11]袁超,袁丹丹,王淼,等.黑茶益生菌种“金花”菌基因组测序及分析[J].食品与药品, 2016, 18(4):259-262.
[2]傅冬和.高通量筛选研究茯砖茶降脂功效[J].茶叶科学,2006,26(3):209-214.
[3]傅冬和.高通量筛选研究茯砖茶对FXR模型的作用[J].食品科学, 2007, 28(5):331-334.
[4]吕嘉枥,郑欣欣,马亚宁.冠突散囊菌液体发酵产降脂类物质的初步研究[J].陕西科技大学学报(自然科学版), 2014, 32(6):115-118.
[5]蔡正安,刘素纯,刘仲华,等.茯砖茶中冠突散囊菌纤维素酶的酶学性质研究[J].茶叶科学, 2010, 30(1):57-62.
[6]李玉婷,吕嘉枥,李良凤,等.冠突散囊菌固态发酵产消化酶活力的研究[J].中国酿造, 2012, 31(10):93-95.
[7]黄群,陈林杰,李彦坡,等.冠突散囊菌黑茶发酵液对消化酶活性影响的研究[J].微生物学通报, 2007, 34(5):917-920.
[8]李莹,邹先伟,张小娜,等.冠突散囊菌不同溶剂提取物体外抗氧化活性研究[J].中国实验方剂学杂志, 2014, 20(7):143-146.
[9]欧阳梅,熊昌云,屠幼英,等.冠突散囊菌对茶叶品质成分及其抗氧化活性影响[J].菌物学报, 2011, 30(2):343-348.
[10]李佳莲,胡博涵,赵勇彪,等.冠突散囊菌发酵液的抑菌作用[J].食品科学, 2011, 32(11):157-160.
[11]袁超,袁丹丹,王淼,等.黑茶益生菌种“金花”菌基因组测序及分析[J].食品与药品, 2016, 18(4):259-262.