不同分子量坛紫菜多糖体内抗氧化活性研究
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摘要
为了解不同分子量坛紫菜多糖的体内抗氧化活性,为低值坛紫菜开发利用技术的进一步优化和应用提供理论依据。采用超滤法将坛紫菜粗多糖进行分离,通过4种不同截留分子量的超滤膜得到5种不同分子量的多糖,对其中体外活性较好的坛紫菜多糖PPⅡ和PPⅣ进行体内抗氧化活性研究。试验结果表明,衰老模型组小鼠在连续注射D-半乳糖60 d后,肝脏组织、脑组织和血液中超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化酶(glutathione peroxidase,GSH-Px)、过氧化氢酶(catalase,CAT)活性均显著下降,丙二醛(malondialdehyde,MDA)含量显著升高,说明该试验中的衰老模型建立成功。PPⅡ和PPⅣ的中剂量组和高剂量组均能不同程度显著提高肝脏组织、脑组织和血液中SOD活力、GSH-Px活力和CAT活力,显著降低MDA含量,表现出较好的抗氧化作用,其中PPⅣ组大部分抗氧化指标明显优于PPⅡ,研究结果将对坛紫菜的深加工提供理论指导。
The aim of the study is to explore the antioxidant activity of Porphyra Haitanensis polysaccharide with different molecular weight in vivo,and therefore provide theoretical basis for the optimizations and application of development techniques of low-value Porphyra Haitanensis. Through ultrafiltration,five polysaccharides with different molecular masses were extracted form four ultrafiltration membranes with different molecular weight cut,PPⅡ and PPⅣ of them were used in antioxidant activity study as their had strong in vivo activity. The results showed that:after 60 days when the mice were injected with D-galactose continuously,the activities of SOD,GSH-Px and CAT in the liver tissue,brain tissue and blood of the mice in the aging model group all decreased significantly. MDA content,on the other had,significantly increased. Which indicated the aging mice model was suited to the study. Moderate and high dose groups of PPⅡ and PPⅣ could significantly improve the SOD,GSH-Px and CAT activities in the liver tissue,brain tissue and blood to varying degrees.Similar doses also reduced the MDA content. Taken together these dates suggest a preferable antioxidant effect.However,most of the antioxidant indexes of PPⅣ group were obviously better than those of PPⅡ group. Above results will benefit to the deep-processing of Porphyra Haitanensis.
The aim of the study is to explore the antioxidant activity of Porphyra Haitanensis polysaccharide with different molecular weight in vivo,and therefore provide theoretical basis for the optimizations and application of development techniques of low-value Porphyra Haitanensis. Through ultrafiltration,five polysaccharides with different molecular masses were extracted form four ultrafiltration membranes with different molecular weight cut,PPⅡ and PPⅣ of them were used in antioxidant activity study as their had strong in vivo activity. The results showed that:after 60 days when the mice were injected with D-galactose continuously,the activities of SOD,GSH-Px and CAT in the liver tissue,brain tissue and blood of the mice in the aging model group all decreased significantly. MDA content,on the other had,significantly increased. Which indicated the aging mice model was suited to the study. Moderate and high dose groups of PPⅡ and PPⅣ could significantly improve the SOD,GSH-Px and CAT activities in the liver tissue,brain tissue and blood to varying degrees.Similar doses also reduced the MDA content. Taken together these dates suggest a preferable antioxidant effect.However,most of the antioxidant indexes of PPⅣ group were obviously better than those of PPⅡ group. Above results will benefit to the deep-processing of Porphyra Haitanensis.
引文
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[2]聂永心.黄伞子实体多糖的分离纯化,结构鉴定及生物活性的研究[D].泰安:山东农业大学,2011
[3]周利亘,王春辉,王君虹,等.紫菜多糖的分离纯化及生物学功能研究进展[J].粮油食品科技,2006,14(4):67-69
[4]张杰,杨旭东,王崴.条斑紫菜多糖对人肝癌Bel7402抗肿瘤作用的初步研究[J].中国食物与营养,2010(8):82-84
[5]Shi J,Cheng C,Zhao H,et al.In vivo anti-radiation activities of the Ulva pertusa polysaccharides and polysaccharide-iron(III)complex[J].International journal of biological macromolecules,2013,60:341-346
[6]郑飞.蓝莓多糖对衰老小鼠运动耐力及抗疲劳效果研究[J].食品科学,2014,35(21):249-252
[7]Zhao Z Y,Zhang Q,Li Y F,et al.Optimization of ultrasound extraction of Alisma orientalis polysaccharides by response surface methodology and their antioxidant activities[J].Carbohydrate Polymers,2015,119:101-109
[8]Zhou C,Yu X,Zhang Y,et al.Ultrasonic degradation,purification and analysis of structure and antioxidant activity of polysaccharide from Porphyra yezoensis Udea[J].Carbohydrate Polymers,2012,87(3):2046-2051
[9]Li C,Huang Q,Fu X,et al.Characterization,antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn.[J].International journal of biological macromolecules,2015,75:298-305
[10]赵婷婷.不同分子量坛紫菜多糖的制备及其抗衰老活性研究[D].青岛:中国科学院研究生院(海洋研究所),2007
[11]郝杰,查学强,鲍素华,等.霍山石斛不同分子量多糖体外抗氧化研究[J].食品科学,2009,30(15):94-98
[12]Marx J L.Oxygen free radicals linked to many diseases[J].Science,1987,4788(235):529-531
[13]鲍素华,查学强,郝杰,等.不同分子量铁皮石斛多糖体外抗氧化活性研究[J].食品科学,2009,30(21):123-127
[14]李广富,陈伟,范路平,等.茶树菇多糖酸奶的抗氧化活性与抗衰老研究[J].现代食品科技,2015,31(10):140-146