基于液相色谱-质谱联用技术的妊娠期糖尿病患者脐带血代谢组学网络分析
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摘要
妊娠期糖尿病(GDM)患病率呈现逐年上升的趋势,探究其对母婴之间代谢影响变得尤为重要。该研究基于液相色谱-质谱的代谢组学技术,分析GDM条件下脐带血血清代谢组的变化。采用正交偏最小二乘判别分析模型(OPLS-DA)与网络模型结合进行分析。结果表明代谢标志物花生四烯酸(AA)为关键代谢网络节点。通路分析显示,GDM会引起不饱和脂肪酸代谢异常,而该通路变化可能是GDM影响母婴代谢的重要分子机制。该文建立的代谢组学网络分析方法为研究GDM母婴间代谢变化提供了新的思路。
The global increase in the prevalence of gestational diabetes mellitus(GDM)in recent years has prompted the study of the effect of GDM on the metabolism between mother and fetus. In this study, the metabolomic investigation of the umbilical cord blood of mothers presenting GDM was performed using liquid chromatography-mass spectrometry(LC-MS), orthogonal projections to latent structures discriminant analysis(OPLS-DA), and network analysis to assess GDM-related metabolic biomarkers. The results showed that arachidonic acid(AA) played an important role in the key metabolic network while further pathway analysis suggested that GDM induced unsaturated fatty acid metabolic disorder. This study provides the underlying metabolic mechanism of GDM-induced metabolic abnormalities between mother and fetus.
The global increase in the prevalence of gestational diabetes mellitus(GDM)in recent years has prompted the study of the effect of GDM on the metabolism between mother and fetus. In this study, the metabolomic investigation of the umbilical cord blood of mothers presenting GDM was performed using liquid chromatography-mass spectrometry(LC-MS), orthogonal projections to latent structures discriminant analysis(OPLS-DA), and network analysis to assess GDM-related metabolic biomarkers. The results showed that arachidonic acid(AA) played an important role in the key metabolic network while further pathway analysis suggested that GDM induced unsaturated fatty acid metabolic disorder. This study provides the underlying metabolic mechanism of GDM-induced metabolic abnormalities between mother and fetus.
引文
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[3] Lu L,Koulman A,Petry C J,et al.Diabetes Care,2016,39(12):2232
[4] Hernandez T L,Van Pelt R E,Anderson M A,et al.Diabetes Care,2016,39(1):39
[5] Ortega-Senovilla H,Alvino G,Taricco E,et al.Diabetes Care,2009,32(1):120
[6] Dani C,Bresci C,Berti E,et al.J Maternal-Fetal Neo M,2014,27(6):537
[7] Radaelli T,Lepercq J,Varastehpour A,et al.Am J Obstet Gynecol,2009,201(2):209.e1
[8] Geng Y,Sun F X,Ma Y,et al.Chinese Journal of Chromatography,2014,32(12):1301 耿越,孙丰霞,马玉,等.色谱,2014,32(12):1301
[9] Mai D T,Yang C,Xue Y,et al.Chinese Journal of Chromatography,2017,35(6):578 麦旦提,杨婵,薛芸,等.色谱,2017,35(6):578
[10] Peng S,Zhang J,Liu L,et al.J Proteome Res,2015,14(4):1799
[11] Dunn W B,Broadhurst D,Begley P,et al.Nat Protoc,2011,6(7):1060
[12] Liu R,Hong J,Xu X,et al.Nat Med,2017,23(7):859
[13] Luan H,Ji F,Chen Y,et al.Anal Chim Acta,2018,1036:66
[14] Torloni M,Betran A,Horta B,et al.Obes Rev,2009,10(2):194
[15] Wang H X,Zhang D T.Chinese Journal of Modern Clinical Medicine,2011,37(4):248 王寒旭,张德太.现代临床医学,2011,37(4):248
[16] Borkman M,Storlien L H,Pan D A,et al.New Engl J Med,1993,328(4):238
[17] Bukowiecka M,Burzynska I,Sansone A,et al.PLoS One,2018,13(9):e0203799