煎药器皿对白芥子煎液中芥子碱含量及其特征图谱的影响
|
摘要
目的:比较4种煎药器皿对白芥子煎液中芥子碱含量及煎液特征图谱的影响,为经典名方的开发优选最佳的煎煮容器。方法:选择传统砂锅、陶瓷锅、圆底烧瓶、不锈钢锅4种煎煮器皿,以白芥子煎液中芥子碱含量及煎液的特征图谱为指标,探究不同煎煮器皿对白芥子指标性成分的含量和煎液特征图谱的影响。采用"中药色谱指纹图谱相似度评价系统"(2004A版)对特征图谱进行相似度评价。结果:传统砂锅、陶瓷锅、圆底烧瓶、不锈钢锅提取的芥子碱质量分数分别为0. 04%,0. 07%,0. 84%,0. 97%。传统砂锅与陶瓷锅、圆底烧瓶、不锈钢锅所制煎液的特征图谱相似度分别为0. 98,0. 82,0. 68;陶瓷锅与圆底烧瓶、不锈钢锅所制煎液的特征图谱的相似度分别为0. 79,0. 62;圆底烧瓶与不锈钢锅所制煎液的特征图谱的相似度0. 97。结论:不同煎煮容器所得白芥子煎液中芥子碱的含量有较大差异,传统砂锅与圆底烧瓶、不锈钢锅所得煎液的特征图谱的相似度较低。
Objective: To compare the effect of four kinds of decocting containers on the content of sinapine and the HPLC specific chromatograms of Sinapis Semen decoction,so as to optimize decocting container for the development of classical formulas. Method: Selecting four kinds of decoction vessels,named traditional casserole,ceramic pot,round-bottom flask and stainless-steel pot as the research object,the content of sinapine in Sinapis Semen decoction and its HPLC specific chromatograms were used as indexes to investigate the influence of different decoction vessels on the decoction. Similarity evaluation of specific chromatograms was performed by the " Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine"(edition of2004 A). Result: The contents of sinapine in the decoction prepared by traditional casserole,ceramic pot,roundbottom flask and stainless-steel pot were 0. 04%,0. 07%,0. 84% and 0. 97%,respectively. Compared with specific chromatograms of the decoction prepared by traditional casserole,the similarities of specific chromatograms of the decoction prepared by ceramic pot,round-bottom flask and stainless-steel pot were 0. 98,0. 82 and 0. 68,respectively. Compared with specific chromatograms of the decoction prepared by ceramic pot,the similarities of specific chromatograms of the decoction prepared by round-bottom flask and stainless-steel pot were 0. 79 and 0. 62,respectively. Compared with specific chromatograms of the decoction prepared by round-bottom flask, the similarity of specific chromatograms of the decoction prepared by stainless-steel pot was 0. 97. Conclusion: The content of sinapine and HPLC specific chromatograms of Sinapis Semen decoction obtained from different decocting containers are quite different.
Objective: To compare the effect of four kinds of decocting containers on the content of sinapine and the HPLC specific chromatograms of Sinapis Semen decoction,so as to optimize decocting container for the development of classical formulas. Method: Selecting four kinds of decoction vessels,named traditional casserole,ceramic pot,round-bottom flask and stainless-steel pot as the research object,the content of sinapine in Sinapis Semen decoction and its HPLC specific chromatograms were used as indexes to investigate the influence of different decoction vessels on the decoction. Similarity evaluation of specific chromatograms was performed by the " Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine"(edition of2004 A). Result: The contents of sinapine in the decoction prepared by traditional casserole,ceramic pot,roundbottom flask and stainless-steel pot were 0. 04%,0. 07%,0. 84% and 0. 97%,respectively. Compared with specific chromatograms of the decoction prepared by traditional casserole,the similarities of specific chromatograms of the decoction prepared by ceramic pot,round-bottom flask and stainless-steel pot were 0. 98,0. 82 and 0. 68,respectively. Compared with specific chromatograms of the decoction prepared by ceramic pot,the similarities of specific chromatograms of the decoction prepared by round-bottom flask and stainless-steel pot were 0. 79 and 0. 62,respectively. Compared with specific chromatograms of the decoction prepared by round-bottom flask, the similarity of specific chromatograms of the decoction prepared by stainless-steel pot was 0. 97. Conclusion: The content of sinapine and HPLC specific chromatograms of Sinapis Semen decoction obtained from different decocting containers are quite different.
引文
[1]国家药品监督管理局.关于发布《古代经典名方中药复方制剂简化注册审批管理规定》的公告(2018年第27号)[EB/OL]. http://cnda. cfda. gov. cn/WS04/CL2050/228247. html,2018-06-01.
[2]王智民,刘菊妍,刘晓谦,等.谈经典名方的化学、生产和质量控制研发和监管[J].中国中药杂志,2017,42(10):1819-1824.
[3]卢芳国,张世鹰,吴治谚,等.中药煎煮的容器、溶媒、时间、火候因素[J].中医杂志,2016,57(1):78-80.
[4]马爱华,张俊慧,赵仲坤,等.容器溶媒热源对中药煎出率的影响[J].时珍国医国药,1997,8(6):501-503.
[5]孙银芳.中药白芥子最新研究进展[J].新中医,2015,47(10):209-211.
[6]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:160-161.
[7]闫兴柱,秦艳虹.秦艳虹应用白芥子散穴位贴敷验案三则[J].世界最新医学信息文摘,2018,18(6):174,177.
[8]杨艳,付昆,徐敏,等.伏九贴敷疗法皮肤刺激性与抗哮喘作用相关性研究[J].辽宁中医药大学学报,2018,20(1):193-196.
[9]赵娟萍,刘剑桥,杨柳,等.白芥子涂法的研究进展[J].中国实验方剂学杂志,2018,24(10):221-226.
[10]马天姝,王富春.穴位敷贴疗法治疗慢性阻塞性肺疾病[J].吉林中医药,2017,37(3):217-220.
[11]万军梅.中药白芥子研究进展[J].中国民族民间医药,2014,23(11):20-22.
[12]刘菊燕,巢建国,谷巍,等.泻肺平喘灵的血清药物化学成分[J].中国实验方剂学杂志,2014,20(17):110-113.
[13]冯文杰,贾晓斌,刘丹.影响煎煮汤剂质量的多因素分析及规范化管理研究[J].中草药,2014,45(16):2422-2426.
[2]王智民,刘菊妍,刘晓谦,等.谈经典名方的化学、生产和质量控制研发和监管[J].中国中药杂志,2017,42(10):1819-1824.
[3]卢芳国,张世鹰,吴治谚,等.中药煎煮的容器、溶媒、时间、火候因素[J].中医杂志,2016,57(1):78-80.
[4]马爱华,张俊慧,赵仲坤,等.容器溶媒热源对中药煎出率的影响[J].时珍国医国药,1997,8(6):501-503.
[5]孙银芳.中药白芥子最新研究进展[J].新中医,2015,47(10):209-211.
[6]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:160-161.
[7]闫兴柱,秦艳虹.秦艳虹应用白芥子散穴位贴敷验案三则[J].世界最新医学信息文摘,2018,18(6):174,177.
[8]杨艳,付昆,徐敏,等.伏九贴敷疗法皮肤刺激性与抗哮喘作用相关性研究[J].辽宁中医药大学学报,2018,20(1):193-196.
[9]赵娟萍,刘剑桥,杨柳,等.白芥子涂法的研究进展[J].中国实验方剂学杂志,2018,24(10):221-226.
[10]马天姝,王富春.穴位敷贴疗法治疗慢性阻塞性肺疾病[J].吉林中医药,2017,37(3):217-220.
[11]万军梅.中药白芥子研究进展[J].中国民族民间医药,2014,23(11):20-22.
[12]刘菊燕,巢建国,谷巍,等.泻肺平喘灵的血清药物化学成分[J].中国实验方剂学杂志,2014,20(17):110-113.
[13]冯文杰,贾晓斌,刘丹.影响煎煮汤剂质量的多因素分析及规范化管理研究[J].中草药,2014,45(16):2422-2426.