规模化驴场中马流感病毒H3N8亚型感染情况调查

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英文篇名：Prevalence of Equine Influenza Virus H3N8 Subtype Infection in Large-scale Donkey Farms 作者：于永峰 ; 夏楠 ; 孙晓波 ; 庞雪晴 ; 刘文强 英文作者：YU Yong-feng;XIA Nan;SUN Xiao-bo;PANG Xue-qing;LIU Wen-qiang;Liaocheng University; 关键词：规模化驴场 ; 马流感病毒 ; H3N8亚型 ; RT-PCR ; 抗体 英文关键词：large-scale donkey farms;;equine influenza virus;;H3N8 subtype;;RT-PCR;;antibody 中文刊名：NMXK 英文刊名：Animal Husbandry and Feed Science 机构：聊城大学; 出版日期：2019-07-05 17:29 出版单位：畜牧与饲料科学 年：2019 期：v.40;No.255 基金：山东省现代农业产业技术体系驴创新团队项目(SDAIT-27);; 国家级大学生科技创新创业训练计划(201810447011);; 聊城大学大学生创新创业训练项目(CXCY2018015) 语种：中文; 页：NMXK201906026 页数：4 CN：06 ISSN：15-1228/S 分类号：94-97

摘要

[目的]调查山东省聊城市规模化驴场中马流感病毒的感染情况,并分析其可能的来源。[方法]从聊城的规模化驴场采集病料和血清,通过HI试验检测驴血清中的马流感病毒H3N8亚型抗体的阳性率。使用RT-PCR技术扩增肺脏和鼻腔棉拭子样品中的马流感病毒M基因,对获得的马流感病毒M基因与不同流感病毒的M基因进行序列比对,推测其来源。[结果]HI试验表明,120个血清样品中马流感病毒H3N8亚型血清抗体阳性率为33.3%(40/120);其中,母驴的马流感病毒H3N8亚型血清抗体阳性率为42.5%(17/40)、公驴为32.5%(13/40)、驴驹为25.0%(10/40)。通过RT-PCR检测发现,32.3%(21/65)的样品可测出目的条带。通过序列比对得出,该试验获得的流感病毒M基因与马属动物的H3N8亚型流感病毒高度同源(CY032222、CY032318、CY028821等),同源性最高可达99.8%。[结论]马流感病毒在聊城周边的数个规模化养驴场发生流行。该研究从驴体内分离的流感病毒M基因属于马流感病毒H3N8亚型M基因。
        [Objective] To investigate the infection status of equine influenza virus in large-scale donkey farms in Liaocheng City of Shandong Province, and to identify the possible sources of the virus. [Method] Clinical samples were collected from the largescale donkey farms in Liaocheng City. The positive rate of serum antibodies against H3 N8 subtype of equine influenza virus was determined by HI test. RT-PCR was used to amplify the M gene of equine influenza virus in lungs and nasal swabs. The obtained M gene was sequenced and compared with the M gene of different influenza viruses to infer its origin. [Result] HI test showed that the positive rate of equine influenza virus H3 N8 subtype in 120 serum samples was 33.3%(40/120). The positive rate for female donkeys, male donkeys and donkey foals was 42.5%(17/40), 32.5%(13/40) and 25.0%(10/40), respectively. RT-PCR assay demonstrated that 32.3%(21/65) of the samples was detected as positive for the presence of M gene. The M gene obtained in this study is highly homologous to H3 N8 subtype influenza virus of equine(CY032222, CY032318, CY028821, etc.), with the highest homology of 99.8%. [Conclusion] Equine influenza virus is prevalent in several large-scale donkey farms around Liaocheng City. The M gene of influenza virus obtained from donkeys in this study belongs to the M gene of H3 N8 subtype of equine influenza virus.

引文

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