牛蒡苷元及牛蒡子提取物的急性毒性试验研究
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摘要
为评价牛蒡苷元及牛蒡子提取物在兽医临床使用中的安全性,根据兽药急性毒性试验(LD50测定)指导原则的要求,开展了牛蒡苷元及牛蒡子提取物在SPF级昆明小鼠经口给药的急性毒性试验研究。试验结果表明:小鼠灌胃给予牛蒡苷元的半数致死量为219.8 mg/kg,死亡均发生在3 h以内,存活动物均正常生长;鼠灌胃给予牛蒡子提取物后采用最大耐受量法测定发现,给药组与对照组雌雄小鼠生长良好,在14 d观察期内均未出现明显中毒症状和死亡情况,药物急性经口MTD≥36 g/kg.bw;根据兽药指导原则经口给药急性毒性试验剂量分级标准,牛蒡苷元属中等毒级,牛蒡子提取物为实际无毒级。
In order to evaluate the safety of arctiin and fructus lappa lappa extract in veterinary clinical use,the acute toxicity test of arctiin and fructus lappa extract in SPF grade kunming mice was conducted according to the requirements of the veterinary acute toxicity test( LD50 test) guidelines. The results showed that the lethal dose of arctium lappa was 219.8 mg/kg,all the deaths occurred within 3 h,and all the surviving animals grew normally.After the mice were given burdock extract by gavage,the method of maximum tolerance was adopted. It was found that both male and female mice in the administration group and the control group grew well,and no obvious poisoning symptoms or death occurred during the 14 d observation period. According to the veterinary drug guidelines for oral administration of acute toxicity test dose grading standards,burdock glycoside is of medium toxic grade,and burdock seed extract is actually non-toxic grade.
In order to evaluate the safety of arctiin and fructus lappa lappa extract in veterinary clinical use,the acute toxicity test of arctiin and fructus lappa extract in SPF grade kunming mice was conducted according to the requirements of the veterinary acute toxicity test( LD50 test) guidelines. The results showed that the lethal dose of arctium lappa was 219.8 mg/kg,all the deaths occurred within 3 h,and all the surviving animals grew normally.After the mice were given burdock extract by gavage,the method of maximum tolerance was adopted. It was found that both male and female mice in the administration group and the control group grew well,and no obvious poisoning symptoms or death occurred during the 14 d observation period. According to the veterinary drug guidelines for oral administration of acute toxicity test dose grading standards,burdock glycoside is of medium toxic grade,and burdock seed extract is actually non-toxic grade.
引文
[1]中国兽药典委员会.中华人民共和国兽药典(2015年版二部)[S].Commission of Chinese veterinary pharmacopoeia. People’s Republic of China Veterinary Pharmacopoeia(twoeditions of 2015)[S].
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[9]张亚君,刘文利,林明明,等.中药复方制剂“归芪饮”的急性毒性研究[J].山东畜牧兽医,2018,1(252):9-10.Zhang Y J,Liu W L,Lin M M,et al. Acute toxicity of guiqi decoction,a traditional Chinese medicine compound preparation[J]. Shandong Animal Husbandry and Veterinary, 2018,1(252):9-10.
[10]秦志华,王淑婷,郭沛,等.银黄连翘散的毒理学研究[J].青岛农业大学学报,2015,32(4):260-264.Qin Z H,Wang S T,Guo P,et al. Study on toxicology of Yinhuanglianqiao Powder[J],Journal of Qingdao Agricultural University,2015,32(4):260-264.
[2]齐艳明.牛蒡化学成分研究[D].齐齐哈尔:齐齐哈尔大学,2012.QI Y M. Study on the chemical constituents of burdock[D].Qiqiha:Qiqihar university,2012.
[3] Cho M K,Jang Y P,Kim Y C,et al. Arctigenin,a phenylpropanoid dibenzylbutyrolactone lignan,inhibits MAP kinases and AP-1 activation via potent MKK inhibition:the role in TNF-a inhibition. Int Immunopharmacol,2004,4(10):1419-1429
[4] Chen J,Li W T,Jin E G,et al.The antiviral activity of arctigenin in traditional Chinese medicine on porcine circovirus type2. Research in Veterinary Science. 2016,6(106):159-164
[5]张兴德,张彩琴,刘启迪,等.牛蒡子抗肿瘤活性成分及作用机制研究进展[J].中国现代中药,2012,14(12):12-15.Zhang X D,Zhang X Q,Liu Q D,et al. Research progress on antitumor constituents and mechanism of Arctium lappa L[J].Modern Chinese Medicine,2012,14(12):12-15.
[6]卢来春.牛蒡子苷分离、纯化及其对实验性糖尿病大鼠血管内皮细胞保护机制的初步探讨[D].重庆:第三军医大学,2007.LU L C. Study on the seperation and purification way of Arctiin from Fructus Arctii and study on the protrctive mechanisms on vascular endothelial cell of experimental diabetic rats with Arctiin[D].Chongqing:Third military medical university,2007.
[7]农业部兽药评审中心.兽药研究技术指导原则汇编[S].Veterinary Drug Evaluation Center of Ministry of Agriculture.Compilation of technical guidelines for veterinary drug research[S].
[8]周德刚,张晓会,刘兴金,等.双黄连口服液的急性毒性试验研究[J].中国兽药杂志,2011,45(12):29-30.Zhou D G,Zhang X H,Liu X J,et al. Research of acute toxicity of Shuanghuanglian Oral Liquid[J]. Chinese Journal of Veterinary Drug,2011,45(12):29-30.
[9]张亚君,刘文利,林明明,等.中药复方制剂“归芪饮”的急性毒性研究[J].山东畜牧兽医,2018,1(252):9-10.Zhang Y J,Liu W L,Lin M M,et al. Acute toxicity of guiqi decoction,a traditional Chinese medicine compound preparation[J]. Shandong Animal Husbandry and Veterinary, 2018,1(252):9-10.
[10]秦志华,王淑婷,郭沛,等.银黄连翘散的毒理学研究[J].青岛农业大学学报,2015,32(4):260-264.Qin Z H,Wang S T,Guo P,et al. Study on toxicology of Yinhuanglianqiao Powder[J],Journal of Qingdao Agricultural University,2015,32(4):260-264.
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