GC一测多评法测定小儿健脾贴膏中丁香酚、异龙脑和龙脑的含量
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摘要
目的建立测定小儿健脾贴膏中丁香酚、异龙脑和龙脑3种成分含量的一测多评法。方法采用GC法,以萘为内标物、丁香酚为内参物,建立该成分与异龙脑和龙脑的相对校正因子,用该校正因子进行3种有效成分的含量测定,与内标法计算值进行比较。结果一测多评法计算值和内标法测得的异龙脑和龙脑的百分含量相对误差均小于2.0%。结论 GC一测多评法用于小儿健脾贴膏中丁香酚、异龙脑和龙脑3个有效成分的含量测定可行、准确。
Objective To establish a quantitative method by GC with quantitative analysis of multi-components by single-marker(QAMS)for the simultaneous determination of the content of eugenol,isoborneol and borneol in Xiaoerjianpi Plaster.Methods GC was used and naphthalene was served as the internal standard.Eugenol was used as the index,the relative correction factors(RCF)were used to calculate the contents of isoborneol,borneol,and the calculated values were compared with that of internal standard method.Results For isoborneol and borneol,the relative error of the calculated value of QAMS and the measured value of internal standard method was less than 2.0%.Conclusion The QAMS method is feasible and credible for the simultaneous determination of the 3 components in Xiaoerjianpi Plaster.
Objective To establish a quantitative method by GC with quantitative analysis of multi-components by single-marker(QAMS)for the simultaneous determination of the content of eugenol,isoborneol and borneol in Xiaoerjianpi Plaster.Methods GC was used and naphthalene was served as the internal standard.Eugenol was used as the index,the relative correction factors(RCF)were used to calculate the contents of isoborneol,borneol,and the calculated values were compared with that of internal standard method.Results For isoborneol and borneol,the relative error of the calculated value of QAMS and the measured value of internal standard method was less than 2.0%.Conclusion The QAMS method is feasible and credible for the simultaneous determination of the 3 components in Xiaoerjianpi Plaster.
引文
[1]WS3-B-1884-95,卫生部药品标准-中药成方制剂:第十册[S].小儿健脾贴膏.
[2]颜晓航,薛玉梅.小儿健脾贴膏质量标准研究[J].中国药业,2011,20(23):26-27.
[3]王智民,高慧敏,付雪涛,等.“一测多评”法中药质量评价模式方法学研究[J].中国中药杂志,2006,31(23):1925-1928.
[4]任睿,陈新国,李磊,等.GC法测定羚黄宝儿丸中麝香酮、冰片、丁香酚的含量[J].世界中医药,2015,10(3):420-422.
[5]成志平.气相色谱法测定肉豆蔻-8散中的丁香酚的含量[J].中国民族医药杂志,2016,8(8):70-72.
[6]郑明,宋剑锋,金情政,等.GC法同时测定丁桂儿脐贴中桂皮醛和丁香酚的含量[J].中国药师,2013,16(9):1345-1347.
[7]刘宏伟,周莉玲.气相色谱法同时测定活络喷雾剂中4种成分的含量[J].中国新药杂志,2009,18(6):556-559.
[8]陈在敏.气相色谱法检测麝珠明目滴眼液中冰片和麝香酮的含量[J].海峡药学,2011,23(3):54-56.
[9]付滢舟,冯有龙,曹玲,等.一测多评法测定速效救心丸中冰片的含量[J].药物分析杂志,2012,32(5):775-778,796.
[10]张伟,胡世强,陈娟,等.GC法测定肚痛健胃整肠丸中薄荷脑、愈创木酚和丁香酚[J].中成药,2013,35(2):286-289.
[11]徐国兵.GC法同时测定复方麝香注射液中薄荷脑、龙脑、百秋李醇和麝香酮的含量[J].中成药,2008,30(6):855-858.
[12]李爱红,陈伟健,胡文军.一测多评法测定银杏叶胶囊中总黄醇苷的含量[J].中国药房,2012,23(36):3446-3448.
[13]郝美玲,籍学伟,王玉华.GC测定八味沉香胶囊中丁香酚含量[J].中成药,2010,32(1):166-168.
[14]王伟炳,朱华军.气相色谱法测定风痛灵中薄荷脑、冰片和樟脑的含量[J].中国中药杂志,2007,32(21):2319-2320.
[15]李彦超,宋汉敏,李振国.GC法同时测定通络祛痛膏中丁香酚等挥发性组分[J].南京中医药大学学报,2012,28(5):479-481.
[16]傅应华.毛细管气相色谱法同时测定少林风湿跌打膏中薄荷脑、水杨酸甲酯和冰片的含量[J].药物分析杂志,2011,31(6):1111-1115.
[17]张宏波,洪波.乙醇回流提取丁香酚的新方法[J].吉林大学自然科学学报,2001,(2):106-108.
[18]杨云,冯卫生.中药化学成分提取分离手册[M].北京:中国中医药出版社,1998:9-10.
[19]邓修.现代工艺与技术在中药制药工程领域中的应用[J].中成药,1999,21(10):537-539.
[2]颜晓航,薛玉梅.小儿健脾贴膏质量标准研究[J].中国药业,2011,20(23):26-27.
[3]王智民,高慧敏,付雪涛,等.“一测多评”法中药质量评价模式方法学研究[J].中国中药杂志,2006,31(23):1925-1928.
[4]任睿,陈新国,李磊,等.GC法测定羚黄宝儿丸中麝香酮、冰片、丁香酚的含量[J].世界中医药,2015,10(3):420-422.
[5]成志平.气相色谱法测定肉豆蔻-8散中的丁香酚的含量[J].中国民族医药杂志,2016,8(8):70-72.
[6]郑明,宋剑锋,金情政,等.GC法同时测定丁桂儿脐贴中桂皮醛和丁香酚的含量[J].中国药师,2013,16(9):1345-1347.
[7]刘宏伟,周莉玲.气相色谱法同时测定活络喷雾剂中4种成分的含量[J].中国新药杂志,2009,18(6):556-559.
[8]陈在敏.气相色谱法检测麝珠明目滴眼液中冰片和麝香酮的含量[J].海峡药学,2011,23(3):54-56.
[9]付滢舟,冯有龙,曹玲,等.一测多评法测定速效救心丸中冰片的含量[J].药物分析杂志,2012,32(5):775-778,796.
[10]张伟,胡世强,陈娟,等.GC法测定肚痛健胃整肠丸中薄荷脑、愈创木酚和丁香酚[J].中成药,2013,35(2):286-289.
[11]徐国兵.GC法同时测定复方麝香注射液中薄荷脑、龙脑、百秋李醇和麝香酮的含量[J].中成药,2008,30(6):855-858.
[12]李爱红,陈伟健,胡文军.一测多评法测定银杏叶胶囊中总黄醇苷的含量[J].中国药房,2012,23(36):3446-3448.
[13]郝美玲,籍学伟,王玉华.GC测定八味沉香胶囊中丁香酚含量[J].中成药,2010,32(1):166-168.
[14]王伟炳,朱华军.气相色谱法测定风痛灵中薄荷脑、冰片和樟脑的含量[J].中国中药杂志,2007,32(21):2319-2320.
[15]李彦超,宋汉敏,李振国.GC法同时测定通络祛痛膏中丁香酚等挥发性组分[J].南京中医药大学学报,2012,28(5):479-481.
[16]傅应华.毛细管气相色谱法同时测定少林风湿跌打膏中薄荷脑、水杨酸甲酯和冰片的含量[J].药物分析杂志,2011,31(6):1111-1115.
[17]张宏波,洪波.乙醇回流提取丁香酚的新方法[J].吉林大学自然科学学报,2001,(2):106-108.
[18]杨云,冯卫生.中药化学成分提取分离手册[M].北京:中国中医药出版社,1998:9-10.
[19]邓修.现代工艺与技术在中药制药工程领域中的应用[J].中成药,1999,21(10):537-539.