DR患者外周血趋化因子MIP-1α和MIP-1β水平变化的临床意义
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摘要
目的:观察糖尿病视网膜病变患者外周血趋化因子巨噬细胞炎症蛋白-1α(macrophage inflammatory protein,MIP-1α)、MIP-1β水平变化,并分析其临床意义。方法:选取佛山市第五人民医院和佛山市南海区第四人民医院收治的糖尿病无视网膜病变患者(DM组)、非增殖期糖尿病视网膜病变患者(NPDR组)和增殖期糖尿病视网膜病变患者(PDR组),各50例;选取50例健康体检者作为对照组。观察不同组别患者外周血趋化因子MIP-1α、MIP-1β水平、血糖情况和细胞因子水平的差异,采用Pearson相关分析法分析糖尿病视网膜病变患者外周血趋化因子MIP-1α、MIP-1β水平与血糖和细胞因子水平的相关性。结果:DM组患者的MIP-1α、MIP-1β和平均血糖(mean blood glucose,MBG)水平均高于对照组,差异有统计学意义(t=6. 306、2. 954、3. 617,均P<0. 05); DM组患者的胰岛素样生长因子-1(insulin like growth factor-1,IGF-1)、成纤维细胞生长因子-21 (fibroblast growth factor-21,FGF-21)、血管内皮生长因子(VEGF)水平均高于对照组,差异有统计学意义(t=27. 216、7. 778、3. 214,均P <0. 05)。PDR组患者的MIP-1α、MIP-1β和MBG水平均高于NPDR组,差异有统计学意义(t=6. 620、3. 461、3. 378,均P<0. 05); PDR组患者的IGF-1、FGF-21、VEGF水平高于NPDR组,差异有统计学意义(t=10. 260、12. 611、4. 108,均P <0. 05)。糖尿病视网膜病变患者的MIP-1α、MIP-1β水平与MBG、IGF-1、FGF-21、VEGF水平呈正相关。结论:增殖期糖尿病视网膜病变患者的MIP-1α、MIP-1β水平较高,且与血糖和细胞因子水平呈正相关。
AIM: To observe the changes of chemokine macrophage inflammatory protein( MIP-1α) and MIP-1β in peripheral blood of patients with diabetic retinopathy and to analyze its clinical significance.METHODS: The patients with diabetic retinopathy( DM group),monproli fertive diabetic retinopathy( NPDR group) and proliferative diabetic retinopathy( PDR group) were selected in Foshan Fifth People's Hospital and the Fourth People's Hospital of Nanhai District,Foshan. There were 50 patients were treated in each group,and 50 healthy persons( control group) were selected for the same period. The differences in the levels of chemokine MIP-1 alpha,MIP-1 beta,blood glucose and cytokines in peripheral blood of patients with different groups were observed. The correlation between the levels of chemokine MIP-1α and MIP-1β ofperipheral blood in patients with diabetic retinopathy and the level of blood glucose and cytokines was analyzed by Pearson correlation analysis.RESULTS: The levels of MIP-1α,MIP-1β and mean blood glucose( MBG) in the DM group were higher than those in the control group( t = 6. 306,2. 954,3. 617; P<0. 05). The levels of insulin like growth factor-1( IGF-1),fibroblast growth factor-21( FGF-21) and vascular endothelial growth factor( VEGF) in DM group were higher than those in control group( t = 27. 216,7. 778,3. 214; P<0. 05). The levels of MIP-1α,MIP-1βand MBG in PDR group were higher than those in NPDR group( t = 6. 620,3. 461,3. 378; P<0. 05). The levels of IGF-1,FGF-21 and VEGF in PDR group were higher than those in NPDR group( t =10. 260,12. 611,4. 108; P<0. 05).The level of MIP-1α and MIP-1β in patients with diabetic retinopathy is positively correlated with the levels of MBG,IGF-1,FGF-21 and VEGF.CONCLUSION: The levels of MIP-1α and MIP-1β in patients with PDR are relatively high,and are positively correlated with blood glucose and cytokines levels.
AIM: To observe the changes of chemokine macrophage inflammatory protein( MIP-1α) and MIP-1β in peripheral blood of patients with diabetic retinopathy and to analyze its clinical significance.METHODS: The patients with diabetic retinopathy( DM group),monproli fertive diabetic retinopathy( NPDR group) and proliferative diabetic retinopathy( PDR group) were selected in Foshan Fifth People's Hospital and the Fourth People's Hospital of Nanhai District,Foshan. There were 50 patients were treated in each group,and 50 healthy persons( control group) were selected for the same period. The differences in the levels of chemokine MIP-1 alpha,MIP-1 beta,blood glucose and cytokines in peripheral blood of patients with different groups were observed. The correlation between the levels of chemokine MIP-1α and MIP-1β ofperipheral blood in patients with diabetic retinopathy and the level of blood glucose and cytokines was analyzed by Pearson correlation analysis.RESULTS: The levels of MIP-1α,MIP-1β and mean blood glucose( MBG) in the DM group were higher than those in the control group( t = 6. 306,2. 954,3. 617; P<0. 05). The levels of insulin like growth factor-1( IGF-1),fibroblast growth factor-21( FGF-21) and vascular endothelial growth factor( VEGF) in DM group were higher than those in control group( t = 27. 216,7. 778,3. 214; P<0. 05). The levels of MIP-1α,MIP-1βand MBG in PDR group were higher than those in NPDR group( t = 6. 620,3. 461,3. 378; P<0. 05). The levels of IGF-1,FGF-21 and VEGF in PDR group were higher than those in NPDR group( t =10. 260,12. 611,4. 108; P<0. 05).The level of MIP-1α and MIP-1β in patients with diabetic retinopathy is positively correlated with the levels of MBG,IGF-1,FGF-21 and VEGF.CONCLUSION: The levels of MIP-1α and MIP-1β in patients with PDR are relatively high,and are positively correlated with blood glucose and cytokines levels.
引文
1 Boniakowski AE, Kimball AS, Jacobs BN, et al. Macrophagemediated inflammation in normal and diabetic wound healing. J Immunol2017; 199(1):17-24
2 Chen S,Li R,Cheng C,et al. Pseudomonas aeruginosa infection alters the macrophage phenotype switching process during wound healing in diabetic mice. Cell Biol Int 2018; 34(6):67-69
3 Lee ES, Lee MY, Kwon M, et al. Sarpogrelate hydrochloride ameliorates diabetic nephropathy associated with inhibition of macrophage activity and inflammatory reaction in db/db mice. PLoS One 2017; 12(6):221-223
4 樊伟,许春香,崔英,等.炎性因子水平与糖尿病肾病分期的关系.中国全科医学2017; 20(S1):21-23
5 覃艳,孙林,刘伏友,等.巨噬细胞介导的免疫炎性反应与糖尿病肾病关系的研究进展.中华肾脏病杂志2017; 33(3):236-240
6 Rojewska E,Zychowska M,Piotrowska A,et al. Involvement of macrophage inflammatory protein-1 family members in the development of diabetic neuropathy and their contribution to effectiveness of morphine.Front Immunol 2018; 9(4):34-36
7 Murai N,Ohtaki H,Watanabe J,et al. Intrapancreatic injection of human bone marrow-derived mesenchymal stem/stromal cells alleviates hyperglycemia and modulates the macrophage state in streptozotocininduced type 1 diabetic mice. PLoS One 2017; 12(10):663-664
8 杨健,杨凤娟,苗晓,等.糖尿病性视网膜病变发生机制研究进展.中国老年学杂志2017; 37(18):4680-4682
9 樊林花,刘建新,李丹,等.糖代谢异常对大鼠肝脏组织中巨噬细胞移动抑制因子及C-Jun氨基端激酶表达水平的影响.中国实验动物学报2015; 23(2):165-170
10 李疏凤,胡健艳,李婷婷,等.糖尿病视网膜病变神经损伤的病理改变及其相关分子机制的研究现状与进展.中华眼底病杂志2017;33(3):312-315
11 何星光,龚兰兰,余才翰.老年糖尿病视网膜病变患者血清ICAM-1、VEGF水平与微血管损伤的相关性分析.海南医学院学报2018; 24(2):235-237
12 朴天华,帅天姣,杨岚.糖尿病性视网膜病变的发生机制及研究进展.牡丹江医学院学报2015; 36(3):102-104
13 王月欣,陈松.糖尿病视网膜病变神经损伤的发病机制和保护防治研究进展.中华眼底病杂志2014; 30(2):209-211
14 Up Noh S, Lee W, Kim W, et al. Expression of macrophage migration inhibitory factor in footpad skin lesions with diabetic neuropathy. Mol Pain 2018; 14(6):844-846
15 吴红,梁尚栋.巨噬细胞对2型糖尿病免疫炎性反应作用的研究进展.重庆医学2015; 44(27):3861-3863
2 Chen S,Li R,Cheng C,et al. Pseudomonas aeruginosa infection alters the macrophage phenotype switching process during wound healing in diabetic mice. Cell Biol Int 2018; 34(6):67-69
3 Lee ES, Lee MY, Kwon M, et al. Sarpogrelate hydrochloride ameliorates diabetic nephropathy associated with inhibition of macrophage activity and inflammatory reaction in db/db mice. PLoS One 2017; 12(6):221-223
4 樊伟,许春香,崔英,等.炎性因子水平与糖尿病肾病分期的关系.中国全科医学2017; 20(S1):21-23
5 覃艳,孙林,刘伏友,等.巨噬细胞介导的免疫炎性反应与糖尿病肾病关系的研究进展.中华肾脏病杂志2017; 33(3):236-240
6 Rojewska E,Zychowska M,Piotrowska A,et al. Involvement of macrophage inflammatory protein-1 family members in the development of diabetic neuropathy and their contribution to effectiveness of morphine.Front Immunol 2018; 9(4):34-36
7 Murai N,Ohtaki H,Watanabe J,et al. Intrapancreatic injection of human bone marrow-derived mesenchymal stem/stromal cells alleviates hyperglycemia and modulates the macrophage state in streptozotocininduced type 1 diabetic mice. PLoS One 2017; 12(10):663-664
8 杨健,杨凤娟,苗晓,等.糖尿病性视网膜病变发生机制研究进展.中国老年学杂志2017; 37(18):4680-4682
9 樊林花,刘建新,李丹,等.糖代谢异常对大鼠肝脏组织中巨噬细胞移动抑制因子及C-Jun氨基端激酶表达水平的影响.中国实验动物学报2015; 23(2):165-170
10 李疏凤,胡健艳,李婷婷,等.糖尿病视网膜病变神经损伤的病理改变及其相关分子机制的研究现状与进展.中华眼底病杂志2017;33(3):312-315
11 何星光,龚兰兰,余才翰.老年糖尿病视网膜病变患者血清ICAM-1、VEGF水平与微血管损伤的相关性分析.海南医学院学报2018; 24(2):235-237
12 朴天华,帅天姣,杨岚.糖尿病性视网膜病变的发生机制及研究进展.牡丹江医学院学报2015; 36(3):102-104
13 王月欣,陈松.糖尿病视网膜病变神经损伤的发病机制和保护防治研究进展.中华眼底病杂志2014; 30(2):209-211
14 Up Noh S, Lee W, Kim W, et al. Expression of macrophage migration inhibitory factor in footpad skin lesions with diabetic neuropathy. Mol Pain 2018; 14(6):844-846
15 吴红,梁尚栋.巨噬细胞对2型糖尿病免疫炎性反应作用的研究进展.重庆医学2015; 44(27):3861-3863