MDCK细胞外基质对HK-2细胞的增殖和迁移的影响
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摘要
目的研究MDCK细胞外基质对HK-2细胞的增殖和迁移的影响。方法用氨水制备MDCK细胞外间质,并且作为HK-2细胞培养基础,随机分为正常对照组和细胞外基质组。用酸性磷酸酶、细胞划痕实验观察细胞的增殖和迁移状况;细胞免疫荧光和Westen blot分析整合素α3和α6蛋白的表达变化。结果与正常对照组比较,MDCK细胞外基质能明显促进HK-2细胞的体外增殖和迁移,整合素α3和α6表达增加(P <0. 05)。结论 MDCK细胞外基质可促进体外培养的HK-2细胞的增殖和迁移。
Objective To study the effects of extracellular matrix on the proliferation and migration of HK-2 cells.Methods The MDCK extracellular matrix was used as the basis for the culture of HK-2 cells and randomly divided into normal control group and extracellular matrix group. The condition of cell proliferation and migration was observed with acid phosphatase and cell scratches; the expression of integrin α3 and α6 protein was analyzed by immunofluorescence and Western blot. Results Compared with normal group,MDCK extracellular matrix significantly promoted the proliferation and migration of HK-2 cells in vitro,and increased the expression of integrin α3 andα6(P < 0. 05). Conclution MDCK extracellular matrix could promote the proliferation and migration of HK-2 cells cultured in vitro.
Objective To study the effects of extracellular matrix on the proliferation and migration of HK-2 cells.Methods The MDCK extracellular matrix was used as the basis for the culture of HK-2 cells and randomly divided into normal control group and extracellular matrix group. The condition of cell proliferation and migration was observed with acid phosphatase and cell scratches; the expression of integrin α3 and α6 protein was analyzed by immunofluorescence and Western blot. Results Compared with normal group,MDCK extracellular matrix significantly promoted the proliferation and migration of HK-2 cells in vitro,and increased the expression of integrin α3 andα6(P < 0. 05). Conclution MDCK extracellular matrix could promote the proliferation and migration of HK-2 cells cultured in vitro.
引文
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[7]袁育珺,段惠芳,胡志坚,等.高糖诱导人肾近曲小管上皮细胞凋亡及其调控机制[J].安徽医科大学学报,2016,51(4):497-501.
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[10]胡红林,王共先.肾缺血再灌注损伤中细胞凋亡和氧化应激[J].中国现代医学杂志,2010,20(15):2279-83.
[11] Chan L S,Wang X S,Lapiere J C,et al. A newly identified 105-kD lower lamina lucida autoantigen is an acidic protein distinct from the 105-kD gamma 2 chain of laminin-5[J]. J Invet Dermatol,1995,105(1):75-9.
[12] Campbell I D,Humphries M J. Integrin structure,activation,and interactions[J]. Cold Spring Harb Perspect Biol,2011,3(3):a004994.
[13]李雪映,孙小然,张宏权.整合素相关微环境调控肿瘤转移[J].生物化学与生物物理进展,2017,44(8):672-7.
[14] Caswell P T,Vadrevu S,Norman J C. Integrins:Masters and slaves of endocytic transport[J]. Nat Rev Mol Cell Biol,2009,10(12):843-53.
[15] Lonqmate W M,Lyons S P,De Freest L,et al. Opposing roles of epidermal integrinsα3β1 andα9β1 in Regulation of m TLD/BMP-1-mediated laminin-γ2 processing during wound healing[J]. J Invest Dermatol,2018,38(2):444-51.
[2] Takahashi T,Friedmacher F,Zimmer J,et al. Decreased expression of integrin subunitsα3,α6,andα8 in the branching airway mesenchyme of nitrofen-induced hypoplastic lungs[J]. Eur J Pediatr Surq,2018,28(1):109-14.
[3] Liao H D,Mao Y,Ying Y G. The involvement of the laminin-integrinα7β1 signaling pathway in mechanical ventilation-induced pulmonary fibrosis[J]. J Thorac Dic,2017,9(10):3961-72.
[4] Schütte K,Schulz C,Link A,et al. Current biomarkers for hepatocellular carcinoma:surveillance,diagnosis and prediction of prognosis[J]. World J Hepatol,2015,7(2):139-49.
[5]汪渊,桂淑玉. 804G细胞外间质对体外培养牛角膜内皮细胞增殖的影响[J].中国药理学通报,1998,14(4):348-50.
[6]张金明,崔永言,徐路生.兔脱细胞尿道基质制备的可行性[J].中国临床康复,2005,9(42):48-9.
[7]袁育珺,段惠芳,胡志坚,等.高糖诱导人肾近曲小管上皮细胞凋亡及其调控机制[J].安徽医科大学学报,2016,51(4):497-501.
[8] De Nicola L,Gabbai F B,Liberti M E,et al. Sodium/glucose cotransporter2 inhibitors and prevention of diabetic nephropathy:targeting the renal tubule in diabetes[J]. Am J Kidney Dis,2014,64(1):16-24.
[9] Guo K,Lu J,Huang Y,et al. Protective role of PGC-1αin diabetic nephropathy is associated with the inhibition of ROS through mitochondrial dynamic remodeling[J]. PLo S One,2015,10(4):e0125176.
[10]胡红林,王共先.肾缺血再灌注损伤中细胞凋亡和氧化应激[J].中国现代医学杂志,2010,20(15):2279-83.
[11] Chan L S,Wang X S,Lapiere J C,et al. A newly identified 105-kD lower lamina lucida autoantigen is an acidic protein distinct from the 105-kD gamma 2 chain of laminin-5[J]. J Invet Dermatol,1995,105(1):75-9.
[12] Campbell I D,Humphries M J. Integrin structure,activation,and interactions[J]. Cold Spring Harb Perspect Biol,2011,3(3):a004994.
[13]李雪映,孙小然,张宏权.整合素相关微环境调控肿瘤转移[J].生物化学与生物物理进展,2017,44(8):672-7.
[14] Caswell P T,Vadrevu S,Norman J C. Integrins:Masters and slaves of endocytic transport[J]. Nat Rev Mol Cell Biol,2009,10(12):843-53.
[15] Lonqmate W M,Lyons S P,De Freest L,et al. Opposing roles of epidermal integrinsα3β1 andα9β1 in Regulation of m TLD/BMP-1-mediated laminin-γ2 processing during wound healing[J]. J Invest Dermatol,2018,38(2):444-51.