鲁中肉羊BMP4基因g.63454744 T>G位点多态性与产羔数的关联分析
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摘要
为了探索骨形态发生蛋白4(Bone morphogenetic protein 4,BMP4)基因g.63454744T>G位点多态性与绵羊产羔数之间的关系,对前期重测序中筛选得到的BMP4基因编码区错义突变单核苷酸多态性位点(Single nucleotide polymorphism site, SNP)g.63454744T>G在384只鲁中肉羊中利用Sequenom MassARRAY~? SNP技术进行分型,再将分型结果与鲁中肉羊产羔数进行关联分析。结果表明:BMP4基因g.63454744T>G位点在鲁中肉羊中存在TT、GT和GG三种基因型;该位点在鲁中肉羊中表现为中度多态(0.250.05)。说明BMP4基因g.63454744T>G位点不适合用于鲁中肉羊产羔数选育。
In order to explore the relationship between the polymorphism of the g.63454744 T>G locus in bone morphogenetic protein 4(BMP4)gene and the litter size in sheep. In previous work,the locus of g.63454744 T>G in BMP4 gene was found by sheep genome resequencing. Then the genotypes of g.63454744 T>G were detected by Sequenom Mass ARRAY~? SNP technique in384 Luzhong Mutton sheep and the association between the mutation and litter size in Luzhong Mutton sheep was analyzed. The result of genotyping showed that three genotypes(TT,GT,GG)were found at g.63454744 T>G in BMP4 gene. Population genetic analysis showed that the g.63454744 T>G locus had a moderate polymorphism(0.25G locus was not under Hardy-Weinberg equilibrium(P<0.05) in this Luzhong Mutton sheep population. Association analysis indicated that the polymorphisms of g.63454744 T>G had no significantly associated with each parity litter size in Luzhong Mutton sheep(P >0.05).Therefore,the polymorphism of the locus of g.63454744 T>G in BMP4 gene had no significant effect on litter size of Luzhong Mutton sheep.
In order to explore the relationship between the polymorphism of the g.63454744 T>G locus in bone morphogenetic protein 4(BMP4)gene and the litter size in sheep. In previous work,the locus of g.63454744 T>G in BMP4 gene was found by sheep genome resequencing. Then the genotypes of g.63454744 T>G were detected by Sequenom Mass ARRAY~? SNP technique in384 Luzhong Mutton sheep and the association between the mutation and litter size in Luzhong Mutton sheep was analyzed. The result of genotyping showed that three genotypes(TT,GT,GG)were found at g.63454744 T>G in BMP4 gene. Population genetic analysis showed that the g.63454744 T>G locus had a moderate polymorphism(0.25
引文
[1]周梅,曹晓涵,贺小云,等.不同产羔数小尾寒羊FOXL2和FIGLA基因的表达研究[J].家畜生态学报,2018(1):14-18.
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[17]苏宁,李予,王文军,等.骨形态发生蛋白-4对人始基卵泡的作用[J].中山大学学报(医学科学版),2008,29(6):676-679.
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[19]Chu M X,Lu L,Feng T,et al.Polymorphism of bone morphogenetic protein 4 gene and its relationship with litter size of Jining Grey goats[J].Molecular Biology Reports,2011,38(7):4315-4320.
[20]孙振梅.黔北麻羊BMP4和BMPR-IB基因克隆表达及其多态性与繁殖性状的关联分析[D].贵阳:贵州大学,2017.
[21]Faure M,Nicol L,Fabre S,et al.BMP-4 inhibits follicle-stimulating hormone secretion in ewe pituitary[J].Journal of Endocrinology,2005,186(1):109-121.
[22]Piper L R,Bindon B M,Davis G H.The single gene inheritance of the high litter size of the Booroola Merino[C]//Land R B,Robinson D W(eds.).Genetics of Reproduction in Sheep.London:Butterworths,1985:115-125.
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[24]Souza C J H,Mac Dougall C,Campbell B K,et al.The Booroola(FecB)phenotype is associated with a mutation in the bone morphogenetic receptor type 1 B(BMPR1B)gene[J].Journal of Endocrinology,2001,169(2):R1-R6.
[25]Guo X,Wang X,Di R,et al.Metabolic effects of FecB gene on follicular fluid and ovarian vein serum in sheep(Ovis aries)[J].International Journal of Molecular Sciences,2018,19(2):539.
[2]徐敏丽,白莉雅,王建英,等.绵羊GDF9基因研究进展[J].山东农业科学,2016,48(5):148-152.
[3]Juengel J L,Davis G H,Mc Natty K P.Using sheep lines with mutations in single genes to better understand ovarian function[J].Reproduction,2013,146(4):R111-R123.
[4]Ampuja M,Kallioniemi A.Transcription factors-Intricate players of the bone morphogenetic protein signaling pathway[J].Genes Chromosomes Cancer,2018,57(1):3-11.
[5]Da C E,De Souza G B,Passos J R,et al.Effects of bone morphogenetic protein 4(BMP4)on in vitro development and survival of bovine preantral follicles enclosed in fragments ovarian tissue[J].Zygote,2017,25(3):256-264.
[6]Ikeda Y,Hasegawa A,Tsubamoto H,et al.Effects of gremlin-2 on the transition of primordial follicles during early folliculogenesis in the human ovary[J].European Journal of Obstetrics&Gynecology&Reproductive Biology,2016,203:72-77.
[7]Wozeny J M,Rosen V,Celeste A J,et al.Novel regulators of bone formation:molecular clones and activities[J].Science,1988,242(4885):1528-1534.
[8]Kijas J W,Menzies M,Ingham A.Sequence diversity and rates of molecular evolution between sheep and cattle genes[J].Animal Genetics,2010,37(2):171-174.
[9]Paulini F,Melo E O.The role of oocyte-secreted factors GDF9 and BMP15 in follicular development and oogenesis[J].Reproduction in Domestic Animals,2011,46(2):354-361.
[10]张驰,周建设,秦楠,等.骨形态发生蛋白4与哺乳动物卵泡发育的研究进展[J].中国畜牧兽医,2010,37(3):141-144.
[11]储明星,周文然,孙少华,等.小尾寒羊BMP4基因多态性及其与高繁殖力关系[J].农业生物技术学报,2008,16(2):237-241.
[12]Pan Z,Li S,Liu Q,et al.Whole-genome sequences of 89 Chinese sheep suggest role of RXFP2 in the development of unique horn phenotype as response to semi-feralization[J].Giga Science,2018,7(4):1-15.
[13]马晓萌,轩俊丽,王慧华,等.TXNRD1基因多态性与乌珠穆沁绵羊生长性状的关联分析[J].畜牧兽医学报,2016,47(5):909-921.
[14]Guo X F,Hu W P,Lang X Z,et al.Two single nucleotide polymorphisms sites inα1-AT gene and their association with somatic cell score in Chinese Holstein cows[J].Journal of Biological Research-Thessaloniki,2017,24:8.
[15]Shimasaki S,Zachow R J,Li D,et al.A functional bone morphogenetic protein system in the ovary[J].PNAS,1999,96(13):7282-7287.
[16]Tan S,Feng B,Yin M,et al.Stromal Senp1 promotes mouse early folliculogenesis by regulating BMP4 expression[J].Cell&Bioscience,2017,7(1):36-48.
[17]苏宁,李予,王文军,等.骨形态发生蛋白-4对人始基卵泡的作用[J].中山大学学报(医学科学版),2008,29(6):676-679.
[18]徐业芬.Smad信号转导通路相关基因表达与湖羊高繁殖力关系的研究[D].南京:南京农业大学,2010.
[19]Chu M X,Lu L,Feng T,et al.Polymorphism of bone morphogenetic protein 4 gene and its relationship with litter size of Jining Grey goats[J].Molecular Biology Reports,2011,38(7):4315-4320.
[20]孙振梅.黔北麻羊BMP4和BMPR-IB基因克隆表达及其多态性与繁殖性状的关联分析[D].贵阳:贵州大学,2017.
[21]Faure M,Nicol L,Fabre S,et al.BMP-4 inhibits follicle-stimulating hormone secretion in ewe pituitary[J].Journal of Endocrinology,2005,186(1):109-121.
[22]Piper L R,Bindon B M,Davis G H.The single gene inheritance of the high litter size of the Booroola Merino[C]//Land R B,Robinson D W(eds.).Genetics of Reproduction in Sheep.London:Butterworths,1985:115-125.
[23]Wilson T,Wu X Y,Juenge J L,et al.Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor(ALK-6)that is expressed in both oocytes and granulosa cells[J].Biology of Reproduction,2001,64(4):1225-1235.
[24]Souza C J H,Mac Dougall C,Campbell B K,et al.The Booroola(FecB)phenotype is associated with a mutation in the bone morphogenetic receptor type 1 B(BMPR1B)gene[J].Journal of Endocrinology,2001,169(2):R1-R6.
[25]Guo X,Wang X,Di R,et al.Metabolic effects of FecB gene on follicular fluid and ovarian vein serum in sheep(Ovis aries)[J].International Journal of Molecular Sciences,2018,19(2):539.