多重耐药鲍曼不动杆菌携带金属酶与整合酶基因特征及同源性分析
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摘要
目的了解吉林地区多重耐药鲍曼不动杆菌(Multidrug-resistant Acinetobacter baumanni,MDRAB)金属酶与整合酶基因的类型,并对菌株进行同源性分析.方法收集吉林地区2所三级甲等医院临床标本分离鉴定的鲍曼不动杆菌38株.多重耐药株携带金属酶的初筛实验-双纸片协同试验:金属酶与整合酶基因的检测-聚合酶链反应(PCR),并对其进行同源性分析.结果在所有MDRAB菌株亚胺培南-EDTA协同试验中,15株(39.4%)阳性,16株(42.1%)携带整合酶基因Ⅰ,2株(5.2%)携带整合酶基因Ⅱ,2株(5.2%)携带blaVIM型金属酶基因,1株(2.6%)携带blaNDM-1型金属酶基因,未检测出金属酶基因blaIMP,blaSIM-1和整合酶基因Ⅲ.结论该地区临床分离的MDRAB耐药率高,可能与其携带金属酶基因和整合酶基因有关,A型MDRAB为医院感染主要流行株.
Objective To explore gene types of metallo-β-lactamases and integrase genes of multi-drug resistant Acinetobacter baumannii(MDRAB) in Jilin area,and to analyze the homology of all the MDRAB.Method 38 strains of MDRAB were isolated from two grade iii-a general hospital in Jilin area. Preliminary screening test of multiple resistant strains carrying metal enzyme-carbapenems-EDTA double disks testing; detection of metal enzyme and integrase gene-polymerase chain reaction(PCR); MDRAB were analyzed for homology by repetitive extragenic palindromic sequence-based PCR.Results 15 strains(39.4%) was positive by Imipenem-EDTA doubles disks test in all MDRAB strains.16 strains(42.1%) carried the intⅠand 2 strains(5.2%) carried the intⅡ.2 strains(5.2%) carried the blaVIMgene,1 strain(2. 6%) carried the blaNDM-1 gene,blaIMP,blaSIM-1 and int Ⅲ genes were undetected.Conclusion The drug resistance rate of clinical isolated MDRAB in this area is high,it may be related to carriage of metallo-β-lactamasesgenes and integrase genes.Type A MDRAB is the main epidemic strain.
Objective To explore gene types of metallo-β-lactamases and integrase genes of multi-drug resistant Acinetobacter baumannii(MDRAB) in Jilin area,and to analyze the homology of all the MDRAB.Method 38 strains of MDRAB were isolated from two grade iii-a general hospital in Jilin area. Preliminary screening test of multiple resistant strains carrying metal enzyme-carbapenems-EDTA double disks testing; detection of metal enzyme and integrase gene-polymerase chain reaction(PCR); MDRAB were analyzed for homology by repetitive extragenic palindromic sequence-based PCR.Results 15 strains(39.4%) was positive by Imipenem-EDTA doubles disks test in all MDRAB strains.16 strains(42.1%) carried the intⅠand 2 strains(5.2%) carried the intⅡ.2 strains(5.2%) carried the blaVIMgene,1 strain(2. 6%) carried the blaNDM-1 gene,blaIMP,blaSIM-1 and int Ⅲ genes were undetected.Conclusion The drug resistance rate of clinical isolated MDRAB in this area is high,it may be related to carriage of metallo-β-lactamasesgenes and integrase genes.Type A MDRAB is the main epidemic strain.
引文
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[8]胡宏章.鲍曼不动杆菌β-内酰胺酶基因型研究及同源性分析[D].南宁:广西医科大学,2009.
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[12]刘原,谭湘淑,韩新鹏.西安地区耐亚胺培南鲍曼不动杆菌的碳青霉烯酶及同源性研究[J].中国感染与化疗杂志,2009,9(1):37-41.
[13]吴春阳,顾国浩,钱雪峰.鲍曼不动杆菌耐药机制及其对策研究的新进展[J].国际检验医学杂志,2013,34(2):174-176.
[14]凌保东.鲍曼不动杆菌抗生素多重耐药性:耐药机制与感染治疗对策[J].中国抗生素杂志,2010,35(4):241-254.
[15] Kouyama Y,Harada S,Ishii Y,et al. Molecular characterization of carbapenem-non-susceptible Acinetobacter spp. in Japan:predominance of multidrug-resistant Acinetobacter baumannii clonal complex 92 and IMPtype metallo-β-lactamase-producing non-baumannii Acinet obacter species[J]. J Infect Chemother,2012,18(4):522-528.
[16] Bonnin R A,Poirel L,Naas T,et al.Dissemination of New Delhi metallo-β-lactamase-1-producing Acinetobacter baumannii in Europe[J]. Clin Microbiol Infect,2012,18(9):E362-E365.
[17] Bakour S,Touati A,Bachiri T,et al. First report of16srRNA methylse Arm A-producing Acinetobacter baumannii[J].J Infect Chemother,2014,20(11):696-701.
[18]傅爱玲,于翠香,李希华,等.多重耐药鲍曼不动杆菌β-内酰胺酶基因和膜孔蛋白基因研究[J].中国抗生素杂志,2013,38(7):549-552.
[2]汪复.多重耐药铜绿假单胞菌与鲍曼不动杆菌严重感染的防治策略[J].中国感染与化疗杂志,2007,7(3):230-232.
[3]唐吉斌,宋有良.整合子与鲍曼不动杆菌多重耐药机制研究进展[J].医学综述,2009,15(7):984-987.
[4]武大伟,马全玲,魏殿军,等.鲍曼不动杆菌ESBLs和金属酶的基因型分析[J].国际检验医学杂志,2009,30(4):323-325.
[5] Chen Yan,Zhihui Zhou,Yan Jiang,et al. Emergence of NDM-1-producing Acinetobacter baumannii in China[J].Journal of Antimicrobial Chemotherapy,2011,66(6):1255-1259.
[6]卢焕.耐碳青霉烯类鲍曼不动杆菌金属酶基因检测及同源性分析[D].南宁:广西医科大学,2014.
[7]黄帆.鲍曼不动杆菌的耐药性与整合子的关系研究[D].南宁:广西医科大学,2014.
[8]胡宏章.鲍曼不动杆菌β-内酰胺酶基因型研究及同源性分析[D].南宁:广西医科大学,2009.
[9] Zong Z,Lu X,Valenzuela J K,et al. An outbreak of carbapenem-resistant Acinetobacter baumannii producing OXA-23 carbapenemase in western China[J].Int J Antimicrob Agents,2008:31(1):50-54.
[10] A mudhan S,Sekar U,Sekar B. OXA beta-lactamasemediated carbapenem resistance in Acinetobacter baumannii[J].Indian Journal of Medical Microbiology,2011,29(3):269-274.
[11] Verma V,Testero S A,Amini K,et al. Hydrolytic mechanism of OXA-58 enzyme,a carbapenem-hydrolyzing class Dβ-lactamase from Acinetobacter baumannii[J].Journal of Biological Chemistry,2011,286(43):37292-37303.
[12]刘原,谭湘淑,韩新鹏.西安地区耐亚胺培南鲍曼不动杆菌的碳青霉烯酶及同源性研究[J].中国感染与化疗杂志,2009,9(1):37-41.
[13]吴春阳,顾国浩,钱雪峰.鲍曼不动杆菌耐药机制及其对策研究的新进展[J].国际检验医学杂志,2013,34(2):174-176.
[14]凌保东.鲍曼不动杆菌抗生素多重耐药性:耐药机制与感染治疗对策[J].中国抗生素杂志,2010,35(4):241-254.
[15] Kouyama Y,Harada S,Ishii Y,et al. Molecular characterization of carbapenem-non-susceptible Acinetobacter spp. in Japan:predominance of multidrug-resistant Acinetobacter baumannii clonal complex 92 and IMPtype metallo-β-lactamase-producing non-baumannii Acinet obacter species[J]. J Infect Chemother,2012,18(4):522-528.
[16] Bonnin R A,Poirel L,Naas T,et al.Dissemination of New Delhi metallo-β-lactamase-1-producing Acinetobacter baumannii in Europe[J]. Clin Microbiol Infect,2012,18(9):E362-E365.
[17] Bakour S,Touati A,Bachiri T,et al. First report of16srRNA methylse Arm A-producing Acinetobacter baumannii[J].J Infect Chemother,2014,20(11):696-701.
[18]傅爱玲,于翠香,李希华,等.多重耐药鲍曼不动杆菌β-内酰胺酶基因和膜孔蛋白基因研究[J].中国抗生素杂志,2013,38(7):549-552.