不同品种大豆蛋白酶抑制剂活性及其对大豆食心虫幼虫的影响
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摘要
选取吉农11、吉农18和吉育47抗性不同的3个大豆品种,研究了大豆蛋白酶抑制剂活性对大豆食心虫幼虫发育历期、幼虫重、危害程度及蛋白酶活性的影响。结果表明:吉农11胰蛋白酶抑制剂活性显著高于其他2个品种,胰凝乳蛋白酶抑制剂活性显著高于吉农18。大豆食心虫为害后,不同品种的大豆蛋白酶抑制剂活性均高于对照;幼虫取食吉农11与取食吉农18和吉育47相比,初期幼虫重明显较低,发育历期延长,幼虫蛀荚率和虫食率均降低;对大豆食心虫抗性表现为吉农11>吉育47>吉农18。取食吉农11的幼虫蛋白酶活性较高,其中胰凝乳蛋白酶活性在不同时间均明显高于取食吉农18的幼虫。大豆蛋白酶抑制剂能够影响大豆食心虫幼虫的生长发育,抑制剂活性越高,表现出的抗虫性越强。
Three soybean varieties,Jinong11,Jinong18 and Jiyu47 with different resistances to Leguminivora glycinivorella,were selected. The effects of soybean proteinase inhibitor activity on developmental duration,weight and damage degree of Leguminivora glycinivorella larvae and protease activity were studied. The results showed that trypsin inhibitor activity of Jinong 11 was significantly higher than that of the other two soybean varieties and chymotrypsin inhibitor activity was significantly higher than that of Jinong18. Protease inhibitor activity of different soybean varieties increased after Leguminivora glycinivorella damage. Compared with Jinong18 and Jiyu 47,larvae fed on Jinong11 had significantly lower weight during initial stage and longer developmental duration. Moreover,rate of harmed pod and rate of harmed seed were all decreased. The resistance to Leguminivora glycinivorella was Jinong11>Jiyu47 >Jinong18. Protease activity of larvae fed on Jinong11 was higher and chymotrypsin activity was significantly higher than that of larvae fed Jinong18 at different time. Soybean protease inhibitors could affect growth and development of Leguminivora glycinivorella larvae.The higher inhibitor activity,the stronger resistance of soybean to the insect.
Three soybean varieties,Jinong11,Jinong18 and Jiyu47 with different resistances to Leguminivora glycinivorella,were selected. The effects of soybean proteinase inhibitor activity on developmental duration,weight and damage degree of Leguminivora glycinivorella larvae and protease activity were studied. The results showed that trypsin inhibitor activity of Jinong 11 was significantly higher than that of the other two soybean varieties and chymotrypsin inhibitor activity was significantly higher than that of Jinong18. Protease inhibitor activity of different soybean varieties increased after Leguminivora glycinivorella damage. Compared with Jinong18 and Jiyu 47,larvae fed on Jinong11 had significantly lower weight during initial stage and longer developmental duration. Moreover,rate of harmed pod and rate of harmed seed were all decreased. The resistance to Leguminivora glycinivorella was Jinong11>Jiyu47 >Jinong18. Protease activity of larvae fed on Jinong11 was higher and chymotrypsin activity was significantly higher than that of larvae fed Jinong18 at different time. Soybean protease inhibitors could affect growth and development of Leguminivora glycinivorella larvae.The higher inhibitor activity,the stronger resistance of soybean to the insect.
引文
[1]付晓霞,徐伟,卢爱军,等.大豆植株挥发物组分变化与大豆食心虫为害的关系[J].吉林农业大学学报,2014,36(4):389-394.
[2]徐伟,付晓霞,田径,等.诱导因子对苜蓿夜蛾和大豆食心虫嗅觉与产卵选择的影响[J].吉林农业大学学报,2010,32(5):488-491,497.
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[4]程媛,韩岚岚,于洪春,等.性诱剂、赤眼蜂和化学药剂协同防治大豆食心虫的研究[J].应用昆虫学报,2016,53(4):752-758.
[5]胡代花,杨晓伟,冯俊涛,等.大豆食心虫性信息素的研究及应用进展[J].农药学学报,2014,16(3):235-244.
[6]史树森,徐伟,臧连生,等.大豆食心虫绿色防控技术[J].大豆科技,2015(4):39-40.
[7]王荣春,孙建华,何述栋,等.胰蛋白酶抑制剂的结构与功能研究进展[J].食品科学,2013,34(9):364-368.
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[9]吕品,柴晓杰,王丕武,等.大豆胰蛋白酶抑制剂KSTI3基因的克隆及其植物表达载体的构建[J].吉林农业大学学报,2007,29(3):275-278.
[10]韦双双,张英霞,贾万贤,等.红肉菠萝蜜种子胰蛋白酶抑制剂的分离纯化及其特性[J].吉林农业大学学报,2013,35(4):420-423.
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[13]王琛柱,项秀芬,张书芳,等.大豆胰蛋白酶抑制剂对棉铃虫幼虫消化生理和生长发育的影响[J].昆虫学报,1995,38(3):272-277.
[14]吴国昭,朱克岩,曾任森.大豆胰蛋白酶抑制剂对斜纹夜蛾生长发育的影响[J].生态环境学报,2013,22(8):1335-1340.
[15]赵爱平,孙聪,展恩玲,等.蛋白酶抑制剂对梨小食心虫幼虫中肠蛋白酶活性的影响[J].昆虫学报,2016,59(10):1069-1078.
[16] Tamhane V A,Giri A P,Sainani M N,et al.Diverse forms of Pin-Ⅱfamily proteinase inhibitors from Capsicum annuum adversely affect the growth and development of Helicoverpa armigera[J].Gene,2007,403(1/2):29-38.
[17]王克勤,李新民,刘春来,等.黑龙江省大豆品种对大豆食心虫抗性评价[J].大豆科学,2006(2):153-157.
[18]王继安,罗秋香.大豆食心虫抗性品种鉴定及抗性性状分析[J].中国油料作物学报,2001,23(2):58-60.
[19]张智勇,齐冰洁,张玉金.大豆不同品种豆荚细胞组织结构与抗食心虫关系的研究[J].内蒙古农业科技,2007(1):39,121.
[20]庄炳昌,岳德荣,王玉民,等.大豆不同品种次生代谢产物及相关酶类含量与抗食心虫的关系[J].中国油料,1992(3):20-22.
[21]樊艳平,张耀文,赵雪英,等.抗豆象绿豆胰蛋白酶抑制剂活性及理化性质[J].作物学报,2017,43(11):1696-1704.
[22] Upadhyay S K,Chandrashekar K. Interaction of salivary and midgut proteins of Helicoverpa armigera with soybean trypsin inhibitor[J]. Protein Journal,2012,31(3):259-264.
[23]钦俊德.植食性昆虫食性的生理基础[J].昆虫学报,1980(1):106-122.
[24] Faeth S H. Indirect interactions between temporally separated herbivores mediated by the host plant[J]. Ecology,1986,67(2):479-494.
[25]王琛柱,钦俊德.植物蛋白酶抑制素抗虫作用的研究进展[J].昆虫学报,1997,40(2):212-218.
[26]宗娜,阎云花,王琛柱.昆虫对植物蛋白酶抑制素的诱导及适应机制[J].昆虫学报,2003,46(4):533-539.
[27]卢晓风,夏玉先,裴炎.植物蛋白酶抑制剂在植物抗虫与抗病中的作用[J].生物化学与生物物理进展,1998(4):39-44.
[28]张钦威.芥菜抗虫性评价及抗性机理研究[D].杭州:浙江农林大学,2017.
[29]张华峰.桉树枝瘿姬小蜂侵害机理及寄主桉树化学防御研究[D].福州:福建农林大学,2013.
[30] Koiwa H,Bressan R A,Hasegawa P M. Regulation of protease inhibitors and plant defense[J]. Trends in Plant Science,1997,2(10):379-384.
[31]于飞,曾鑫年,熊忠华,等.蛋白酶抑制剂在害虫防治中的研究与应用[J].植物保护,2004(3):13-17.
[32]刘会香,张星耀.植物蛋白酶抑制剂及其在林木抗虫基因工程中的应用[J].林业科学,2005(3):148-157.
[2]徐伟,付晓霞,田径,等.诱导因子对苜蓿夜蛾和大豆食心虫嗅觉与产卵选择的影响[J].吉林农业大学学报,2010,32(5):488-491,497.
[3]史树森,崔娟,徐伟,等.温度对大豆食心虫卵和幼虫生长发育的影响[J].中国油料作物学报,2014,36(2):250-255.
[4]程媛,韩岚岚,于洪春,等.性诱剂、赤眼蜂和化学药剂协同防治大豆食心虫的研究[J].应用昆虫学报,2016,53(4):752-758.
[5]胡代花,杨晓伟,冯俊涛,等.大豆食心虫性信息素的研究及应用进展[J].农药学学报,2014,16(3):235-244.
[6]史树森,徐伟,臧连生,等.大豆食心虫绿色防控技术[J].大豆科技,2015(4):39-40.
[7]王荣春,孙建华,何述栋,等.胰蛋白酶抑制剂的结构与功能研究进展[J].食品科学,2013,34(9):364-368.
[8] Abd El-Hady E A,Habiba R A. Effect of soaking and extrusion conditions on antinutrients and protein digestibility of legume seeds[J]. LWT-Food Science and Technology,2003,36(3):285-293.
[9]吕品,柴晓杰,王丕武,等.大豆胰蛋白酶抑制剂KSTI3基因的克隆及其植物表达载体的构建[J].吉林农业大学学报,2007,29(3):275-278.
[10]韦双双,张英霞,贾万贤,等.红肉菠萝蜜种子胰蛋白酶抑制剂的分离纯化及其特性[J].吉林农业大学学报,2013,35(4):420-423.
[11] Schuler T H,Poppy G M,Kerry B R,et al. Insect resistant transgenic plants[J].Trends in Biotechnology,1998,16(4):168-175.
[12]柳武革,薛庆中.蛋白酶抑制剂及其在抗虫基因工程中的应用[J].生物技术通报,2000(1):21-24.
[13]王琛柱,项秀芬,张书芳,等.大豆胰蛋白酶抑制剂对棉铃虫幼虫消化生理和生长发育的影响[J].昆虫学报,1995,38(3):272-277.
[14]吴国昭,朱克岩,曾任森.大豆胰蛋白酶抑制剂对斜纹夜蛾生长发育的影响[J].生态环境学报,2013,22(8):1335-1340.
[15]赵爱平,孙聪,展恩玲,等.蛋白酶抑制剂对梨小食心虫幼虫中肠蛋白酶活性的影响[J].昆虫学报,2016,59(10):1069-1078.
[16] Tamhane V A,Giri A P,Sainani M N,et al.Diverse forms of Pin-Ⅱfamily proteinase inhibitors from Capsicum annuum adversely affect the growth and development of Helicoverpa armigera[J].Gene,2007,403(1/2):29-38.
[17]王克勤,李新民,刘春来,等.黑龙江省大豆品种对大豆食心虫抗性评价[J].大豆科学,2006(2):153-157.
[18]王继安,罗秋香.大豆食心虫抗性品种鉴定及抗性性状分析[J].中国油料作物学报,2001,23(2):58-60.
[19]张智勇,齐冰洁,张玉金.大豆不同品种豆荚细胞组织结构与抗食心虫关系的研究[J].内蒙古农业科技,2007(1):39,121.
[20]庄炳昌,岳德荣,王玉民,等.大豆不同品种次生代谢产物及相关酶类含量与抗食心虫的关系[J].中国油料,1992(3):20-22.
[21]樊艳平,张耀文,赵雪英,等.抗豆象绿豆胰蛋白酶抑制剂活性及理化性质[J].作物学报,2017,43(11):1696-1704.
[22] Upadhyay S K,Chandrashekar K. Interaction of salivary and midgut proteins of Helicoverpa armigera with soybean trypsin inhibitor[J]. Protein Journal,2012,31(3):259-264.
[23]钦俊德.植食性昆虫食性的生理基础[J].昆虫学报,1980(1):106-122.
[24] Faeth S H. Indirect interactions between temporally separated herbivores mediated by the host plant[J]. Ecology,1986,67(2):479-494.
[25]王琛柱,钦俊德.植物蛋白酶抑制素抗虫作用的研究进展[J].昆虫学报,1997,40(2):212-218.
[26]宗娜,阎云花,王琛柱.昆虫对植物蛋白酶抑制素的诱导及适应机制[J].昆虫学报,2003,46(4):533-539.
[27]卢晓风,夏玉先,裴炎.植物蛋白酶抑制剂在植物抗虫与抗病中的作用[J].生物化学与生物物理进展,1998(4):39-44.
[28]张钦威.芥菜抗虫性评价及抗性机理研究[D].杭州:浙江农林大学,2017.
[29]张华峰.桉树枝瘿姬小蜂侵害机理及寄主桉树化学防御研究[D].福州:福建农林大学,2013.
[30] Koiwa H,Bressan R A,Hasegawa P M. Regulation of protease inhibitors and plant defense[J]. Trends in Plant Science,1997,2(10):379-384.
[31]于飞,曾鑫年,熊忠华,等.蛋白酶抑制剂在害虫防治中的研究与应用[J].植物保护,2004(3):13-17.
[32]刘会香,张星耀.植物蛋白酶抑制剂及其在林木抗虫基因工程中的应用[J].林业科学,2005(3):148-157.