牛奶蛋白改性纤维免疫层析快速鉴定方法

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英文篇名：Rapid immunochromatographic identification methods for modified milk protein fibers 作者：张世伟 ; 王云发 ; 王士峰 ; 姚添淇 ; 冯荣虎 ; 杨国武 英文作者：Zhang Shiwei;Wang Yunfa;Wang Shifeng;Yao Tianqi;Feng Ronghu;Yang Guowu;Shenzhen Academy of Metrology and Quality Inspection,National Nutrition Food Testing Center; 关键词：牛奶蛋白改性纤维 ; 免疫层析法 ; 纤维鉴定 ; 特征肽段 英文关键词：modified milk protein fiber;;immunochromatography;;fiber identification;;characteristic peptide 中文刊名：CYYF 英文刊名：Technical Textiles 机构：深圳市计量质量检测研究院国家营养食品质量监督检验中心; 出版日期：2019-04-25 出版单位：产业用纺织品 年：2019 期：v.37;No.367 基金：国家质检总局科技计划资助项目(2017QK095) 语种：中文; 页：CYYF201904013 页数：6 CN：04 ISSN：31-1595/TS 分类号：48-53

摘要

建立了一种快速、简单、准确的牛奶蛋白改性纤维鉴定方法。使用ESI(Electron Spray Ionization)/TOF(Time of Flight)质谱对牛奶蛋白改性纤维胰蛋白酶解肽进行分析,确定了牛奶蛋白改性纤维特征肽段,使用该特征肽段制备得到特异性单克隆抗体;将酪蛋白包被于T线,建立了针对牛奶蛋白改性纤维的免疫层析快速鉴定方法。该方法以7M尿素为提取剂,检出限为氨基酸质量分数为1.00%的牛奶蛋白改性纤维(相当于混纺4.00%牛奶蛋白改性纤维的纱线),检测时间不超过20 min(含前处理)。该方法无需使用分析仪器,对常见的纤维没有非特异性识别,纤维的染色不会对检测结果造成干扰。
        A rapid, simple and accurate identification method for modified milk protein fibers was developed. The digested modified milk protein fiber was characterized by ESI(electron spray ionization)/TOF(time of flight) mass spectrometry and a characteristic peptide hapten was designed. Then the hapten was used to prepare special monoclonal antibody. The immunochromatographic identification method for modified milk protein fibers was developed by wrapping the casein on T line. The limit of detection(LOD) of the method was 1.00% mass fraction of amino acid of modified milk protein fiber(corresponding to the yarn blending with 4.00% modified milk protein fiber) with 7 M urea as an extraction solution. The total test time was less than 20 min. The method didn't need any analytical equipment and would not disturbe the fiber dyeing.

引文

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