马乳酒样乳杆菌(XL10)的分离、鉴定及对小鼠肠道菌群的影响
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摘要
以西藏开菲尔粒为原料,从中筛选出一株马乳酒样乳杆菌XL10,对其进行鉴定及生长特性研究;并以小鼠为研究对象,观察XL10对小鼠肠道菌群的影响。结果表明XL10菌株能够促进小鼠肠道有益菌生长,抑制有害菌繁殖。结果表明XL10菌株具有能够调节肠道菌群平衡,保护肠道健康的潜能。
Lactobacillus kefiranofaciens XL10 was screened from the Tibet Kefir grains. The growth characteristics and the effect of Lactobacillus kefiranofaciens XL10 on gut microbiota of mice were studied. The results showed that Lactobacillus kefiranofaciens XL10 could promote the growth of beneficial bacteria in the gut and inhibit the reproduction of harmful bacteria. In conclusion,Lactobacillus kefiranofaciens XL10 had the potential to regulate the balance of gut microbiota and protect the intestinal health.
Lactobacillus kefiranofaciens XL10 was screened from the Tibet Kefir grains. The growth characteristics and the effect of Lactobacillus kefiranofaciens XL10 on gut microbiota of mice were studied. The results showed that Lactobacillus kefiranofaciens XL10 could promote the growth of beneficial bacteria in the gut and inhibit the reproduction of harmful bacteria. In conclusion,Lactobacillus kefiranofaciens XL10 had the potential to regulate the balance of gut microbiota and protect the intestinal health.
引文
[1]Gao J,Gu F,Ruan H,et al.Culture conditions optimization of Tibetan kefir grains by response surface methodology[J].Procedia Engineering,2012,37(1):132-136
[2]Letie A M,Mayo B,Rachid C T,et al.Assessment of the microbial diversity of Brazilian kefir grainsby PCR-DGGE and pyrosequencing analysis[J].Food Microbiology,2012,31(2):215-221
[3]Wang S Y,Chen K N,Lo Y M,et al.Investigation of microorganisms involved in biosynthesis of the kefir grain[J].Food Microbiology,2012,32(2):1-12
[4]Hong W S,Chen Y P,Chen M J.The antiallergic effect of kefir lactobacilli[J].Food Sci,2010,75(8):H244-H253
[5]Hong W S,Chen Y P,Dai T Y,et al.Effect of heat-inactivated kefir-isolated Lactobacillus kefiranofaciens M1 on preventing an allergic airway response in mice[J].Journal of agricultural and food chemistry,2011,59(16):9022-9031
[6]Wang S Y,Ho Y F,Chen Y P,et al.Effects of a novel encapsulating technique on the temperature tolerance and anti-colitis activity of the probiotic bacterium Lactobacillus kefiranofaciens M1[J].Food Microbiology,2015,46:494-500
[7]Xing Z,Tang W,Yang Y,et al.Colonization and Gut Flora Modulation of Lactobacillus kefiranofaciens ZW3 in the Intestinal Tract of Mice[J].Probiotics and Antimicrobial Proteins,2017,10(2):1-9
[8]Rosenberg M,Gutnick D,Rosenberg E.Adherence of bacteria to hydrocarbons:A simple method for measuring cell-surface hydrophobicity[J].FEMS Microbiology Letters,1980,9(1):29-33
[9]Ward L J H,Brown J C S,Davey G P.Two methods for the genetic differentiation of Lactococcus lactis ssp.lactis and cremoris based on differences in the 16S rRNA gene sequence[J].FEMS Microbiology Letters,1998,166(1):15-20
[10]Hernandez-Sanchez H,Melgar-Lalanne G,Rivera-Espinoza Y,et al.Cell surface properties of halotolerant probiotic lactobacilli[J].Biotechnology advances,2015,4(3):404-413
[11]Ley R E,Turnbaugh P J,Klein S,et al.Microbial ecology:human gut microbes associated with obesity[J].Nature,2006,444(7122):1022-1023
[12]Schwiertz A,Taras D,Sch覿fer K,et al.Microbiota and SCFA in lean and overweight healthy subjects[J].Obesity,2009,18(1):190-195
[13]Arumugam M,Raes J,Pelletier E,et al.Enterotypes of the human gut microbiome[J].Nature,2011,473:174-180
[14]Amy B,Lucy S,Jeffrey B,et al.Untangling the genetic basis of fibrolytic specialization by Lachnospiraceae and Ruminococcaceae in diverse gut communities.Diversity,2013,5:627-640
[15]Kyung-Ah K,Wan G,In-Ah L,et al.High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the tlr4signaling pathway[J].PLoS ONE,2012,7(10):e47713
[16]Alkadhi S,Kunde D,Cheluvappa R,et al.The murine appendiceal microbiome is altered in spontaneous colitis and its pathological progression[J].Gut Pathogens,2014,6(1):25
[17]杨东升.益生菌双歧杆菌作用机制的研究[J].食品研究与开发,2004,25(4):61-65
[18]Liang X,Bushman F D,Fitzgerald G A.Rhythmicity of the intestinal microbiota is regulated by gender and the host circadian clock[J].Proceedings of the National Academy of Sciences,2015,112(33):10479-10484
[19]Christensen H,Kuhnert P.Pasteurellaceae:biology,genomics and molecular aspects[M].Norfolk:Caister Academic Press,2008
[20]Boeckaert C,Vlaeminck B,Dijkstra J.Accumulation of trans C18:1fatty acids in the rumen after dietary algal supplementation is associated with changes in the Butyrivibrio community[J].Applied microbiology,2008,74(22):6923-6930
[2]Letie A M,Mayo B,Rachid C T,et al.Assessment of the microbial diversity of Brazilian kefir grainsby PCR-DGGE and pyrosequencing analysis[J].Food Microbiology,2012,31(2):215-221
[3]Wang S Y,Chen K N,Lo Y M,et al.Investigation of microorganisms involved in biosynthesis of the kefir grain[J].Food Microbiology,2012,32(2):1-12
[4]Hong W S,Chen Y P,Chen M J.The antiallergic effect of kefir lactobacilli[J].Food Sci,2010,75(8):H244-H253
[5]Hong W S,Chen Y P,Dai T Y,et al.Effect of heat-inactivated kefir-isolated Lactobacillus kefiranofaciens M1 on preventing an allergic airway response in mice[J].Journal of agricultural and food chemistry,2011,59(16):9022-9031
[6]Wang S Y,Ho Y F,Chen Y P,et al.Effects of a novel encapsulating technique on the temperature tolerance and anti-colitis activity of the probiotic bacterium Lactobacillus kefiranofaciens M1[J].Food Microbiology,2015,46:494-500
[7]Xing Z,Tang W,Yang Y,et al.Colonization and Gut Flora Modulation of Lactobacillus kefiranofaciens ZW3 in the Intestinal Tract of Mice[J].Probiotics and Antimicrobial Proteins,2017,10(2):1-9
[8]Rosenberg M,Gutnick D,Rosenberg E.Adherence of bacteria to hydrocarbons:A simple method for measuring cell-surface hydrophobicity[J].FEMS Microbiology Letters,1980,9(1):29-33
[9]Ward L J H,Brown J C S,Davey G P.Two methods for the genetic differentiation of Lactococcus lactis ssp.lactis and cremoris based on differences in the 16S rRNA gene sequence[J].FEMS Microbiology Letters,1998,166(1):15-20
[10]Hernandez-Sanchez H,Melgar-Lalanne G,Rivera-Espinoza Y,et al.Cell surface properties of halotolerant probiotic lactobacilli[J].Biotechnology advances,2015,4(3):404-413
[11]Ley R E,Turnbaugh P J,Klein S,et al.Microbial ecology:human gut microbes associated with obesity[J].Nature,2006,444(7122):1022-1023
[12]Schwiertz A,Taras D,Sch覿fer K,et al.Microbiota and SCFA in lean and overweight healthy subjects[J].Obesity,2009,18(1):190-195
[13]Arumugam M,Raes J,Pelletier E,et al.Enterotypes of the human gut microbiome[J].Nature,2011,473:174-180
[14]Amy B,Lucy S,Jeffrey B,et al.Untangling the genetic basis of fibrolytic specialization by Lachnospiraceae and Ruminococcaceae in diverse gut communities.Diversity,2013,5:627-640
[15]Kyung-Ah K,Wan G,In-Ah L,et al.High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the tlr4signaling pathway[J].PLoS ONE,2012,7(10):e47713
[16]Alkadhi S,Kunde D,Cheluvappa R,et al.The murine appendiceal microbiome is altered in spontaneous colitis and its pathological progression[J].Gut Pathogens,2014,6(1):25
[17]杨东升.益生菌双歧杆菌作用机制的研究[J].食品研究与开发,2004,25(4):61-65
[18]Liang X,Bushman F D,Fitzgerald G A.Rhythmicity of the intestinal microbiota is regulated by gender and the host circadian clock[J].Proceedings of the National Academy of Sciences,2015,112(33):10479-10484
[19]Christensen H,Kuhnert P.Pasteurellaceae:biology,genomics and molecular aspects[M].Norfolk:Caister Academic Press,2008
[20]Boeckaert C,Vlaeminck B,Dijkstra J.Accumulation of trans C18:1fatty acids in the rumen after dietary algal supplementation is associated with changes in the Butyrivibrio community[J].Applied microbiology,2008,74(22):6923-6930