黄淮海地区玉蜀黍黑粉菌群体遗传结构分析
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摘要
利用17个多态性ISSR引物对2017年在我国黄淮海地区采集的41株玉蜀黍黑粉菌Ustilago maydis样本进行了种群遗传结构分析。基于UPGMA法和贝叶斯模型对玉蜀黍黑粉菌群体遗传结构分析显示,采集的样本可以分为两大群,且种群的划分表现出了与来源地理纬度的一致性。因此按照各样本所属地理纬度将样本分为北纬33°–34°、北纬35°–36°、北纬37°–38°3大种群。结果表明,分离自北纬33°–34°地区的玉蜀黍黑粉菌遗传多样性最丰富,Nei’s基因多样性(H)为0.2216,Shannon指数(I)为0.3300。分子方差分析(AMOVA)显示,变异中来自种群内部的变异为86%,种群间变异14%(φpt=0.141)。
To determine the population genetic structure of Ustilago maydis, 41 isolates collected from Huang-Huai-Hai region in 2017 were analyzed by using 17 polymorphic ISSR primers. The unweighted pair group method with arithmetic mean(UPGMA) and the Bayesian analysis were used to analyse the population genetic structure of the collected isolates. It was indicated that the isolates could be divided into two geographical groups, and the groups showed clear relationship with the geographical origin of the isolates. Based on demarcation of geographical latitude, the isolates were clustered into 3 groups, 33°–34° N group, 35°–36° N group and 37°–38° N group. The genetic diversity of the 33°–34° N group was the highest. Nei's gene diversity(H)and Shannon index(I) was 0.2216 and 0.3300, respectively. An estimation of the genetic diversity using analysis of molecular variance(AMOVA) revealed that the highest variance mainly formed in intrapopulation(86%), and subordinately interpopulation(14%)(φpt=0.141).
To determine the population genetic structure of Ustilago maydis, 41 isolates collected from Huang-Huai-Hai region in 2017 were analyzed by using 17 polymorphic ISSR primers. The unweighted pair group method with arithmetic mean(UPGMA) and the Bayesian analysis were used to analyse the population genetic structure of the collected isolates. It was indicated that the isolates could be divided into two geographical groups, and the groups showed clear relationship with the geographical origin of the isolates. Based on demarcation of geographical latitude, the isolates were clustered into 3 groups, 33°–34° N group, 35°–36° N group and 37°–38° N group. The genetic diversity of the 33°–34° N group was the highest. Nei's gene diversity(H)and Shannon index(I) was 0.2216 and 0.3300, respectively. An estimation of the genetic diversity using analysis of molecular variance(AMOVA) revealed that the highest variance mainly formed in intrapopulation(86%), and subordinately interpopulation(14%)(φpt=0.141).
引文
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Evanno G,Regnaut S,Goudet J,2005.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study.Molecular Ecology,14(8):2611-2620
Gan QH,Wang M,Zhang SP,Wang FG,Zhao JR,Zheng YL,Xiao YN,2008.Analysis on genetic and genotypic diversity of populations of Sporisorium reilianum in northern China.Chinese Journal of Biochemistry and Molecular Biology,24(9):846-853(in Chinese)
Guo SB,Huang LL,Kang ZS,Cheng JJ,Lu NH,Yang ZW,Chen YC,2009.Efficacy and mechanism of control of wheat stripe rust by diversifying cultivars in mix-planting.Scientia Agricultura Sinica,10:3485-3492
Guo YY,Chen MG,Sun SL,Wu XF,Jiang K,Zhu ZD,Li HJ,He YQ,Wang XM,2013.Genetic diversity of Puccinia polysora Underw.in China.Scientia Agricultura Sinica,46(21):4523-4533(in Chinese)
Hartl DL,Clark AG,2015.Principles of population genetics.Journal of Animal Breeding&Genetics,117(3):143-144
He XH,Yang J,Wang YY,Zhou HP,Chen JB,Li ZS,Li Y,Zhu YY,2003.Annalysis of genetic structure of Magnaporthe grisea in the fields of different rice varieties.Chinese Journal of Applied Ecology,14(5):733-736(in Chinese)
Jiménez-Becerril MF,Hernández-Delgado S,Solís-Oba M,Juan M,González P,2016.Analysis of mitochondrial genetic diversity of Ustilago maydis in Mexico.Mitochondrial DNA Part A,29(1):1-8
Lai GD,Liu WH,Zhang ZH,Feng X,Lin YL,Liu SC,Qi FB,Lai ZX,2013.Genetic structure of natural populations revealed by ISSR in Myrica rubra from Daiyunshan Mountains.Chinese Journal of Tropical Crops,34(10):1863-1870(in Chinese)
Li HQ,Zhang XW,Jiang XW,Shao XY,Zhao YM,Zhao MA,Song XY,Wang JH,2017.Study on seed quality of maize cultivated varieties in 2016.Seed,36(7):87-91(in Chinese)
Liang JM,2014.Analysis of population genetic structure and regional relationship of Puccinia striiformis f.sp.tritici in Gansu,Sichuan,Ningxia and Shaanxi Provinces of China.PhD Dissertation,China Agricultural University,Beijing.81-87(in Chinese)
Ma HX,Sun H,Guo N,Zhang HJ,Shi J,Chang JY,2018.Analysis of toxigenic chemotype and genetic diversity of the Fusarium graminearum species complex.Scientia Agricultura Sinica,51(1):82-95(in Chinese)
Martínez-Espinoza AD,León-Ramírez CG,Singh N,Ruiz-Herrera J,2003.Use of PCR to detect infection of differentially susceptible maize cultivars using Ustilago maydis strains of variable virulence.International Microbiology:the Official Journal of the Spanish Society for Microbiology,6(2):117-120
Nei M,1993.Analysis of gene diversity in subdivided populations.Proceedings of the National Academy of Sciences,70:3321-3323
Peakall R,Smouse PE,2006.GENALEX 6:genetic analysis in Excel.Population genetic software for teaching and research.Molecular Ecology Notes,6:288-295
Peakall R,Smouse PE,2012.GenAlEx 6.5:genetic analysis in Excel.Population genetic software for teaching and research-an update.Bioinformatics,28:2537-2539
Shi J,2009.Study on Ustilago maydis biology and resistant identification of maize.Master Thesis,Gansu Agriculture University,Lanzhou.1-12(in Chinese)
Shi J,Wang ZY,He KL,2005.Changes and occurrence trend of corn diseases and insect pests in Huang-Huai-Hai summer corn regions.Plant Disease,31(5):63-65(in Chinese)
Sun XS,Long ZW,Song GP,Chen CQ,2017.Effects of climate change on cropping pattern and yield of summer maize-winter wheat in Huang-Huai-Hai Plain.Scientia Agricultura Sinica,50(13):2476-2487(in Chinese)
Wachira FN,Waugh R,Hacker CA,Powell W,1995.Detection of genetic diversity in tea(Camellia sinensis)using RAPDmarkers.Genome,38:201-210
Wang JF,Lu NH,Chen CQ,Zhan GM,Huang LL,Kang ZS,2013.Analysis of population genetic structure of Puccinia striiformis f.sp.tritici in Shaanxi Province,China.Acta Phytopathologica Sinica,43(3):294-300(in Chinese)
Yan HH,Wang Y,Zhang RH,Xia SC,Wang ZK,Song XY,2018.Genetic diversity and deduction of primary infection source of Puccinia polysora in Shandong Province based on ISSR-PCR.Mycosystema,37(2):157-165(in Chinese)
Yang H,2009.Study on genetic diversity of Yunnan glutinous rice.PhD Dissertation,Yunnan Agricultural University,Kunming.24-25(in Chinese)
Yeh FC,Yang RC,Boyle TB,1997.Popgene,the user-friendly shareware for population genetic analysis.University of Alberta,Canada.112-118
Zhang CM,Liu YY,Shi J,Zhang LZ,2005.Inoculation technique for identification of corn resistance to common smut.Journal of Maize Sciences,13(3):109-111(in Chinese)
Zhang MJ,Chen YP,2015.Development of genomic SSRmarkers and analysis of genetic diversity of 40 haploid isolates of Ustilago maydis in China.International Journal of Agriculture and Biology,7:14-30
甘琴华,王明,张绍鹏,王凤格,赵久然,郑用琏,肖炎农,2008.北方玉米丝轴黑粉菌遗传多样性与遗传分化研究.中国生物化学与分子生物学报,24(9):846-853
郭云燕,陈茂功,孙素丽,武小菲,江凯,朱振东,李洪杰,何月秋,王晓明,2013.中国玉米南方锈病病原菌遗传多样性.中国农业科学,46(21):4523-4533
何霞红,杨静,王云月,周惠萍,陈建斌,李作森,李炎,朱有勇,2003.水稻品种多样性田间稻瘟病菌群体遗传结构分析.应用生态学报,14(5):733-736
赖恭梯,刘炜婳,张梓浩,冯新,林玉玲,刘生财,祁芳斌,赖钟雄,2013.戴云山野生杨梅自然群体遗传结构的ISSR分析.热带作物学报,34(10):1863-1870
李贺勤,张晓文,江绪文,邵晓宇,赵延明,赵美爱,宋希云,王建华,2017.2016年中国玉米主产区栽培品种种子质量研究--以黄淮海-华北-西南三区为例.种子,36(7):87-91
梁俊敏,2014.中国甘、川、宁、陕地区小麦条锈菌群体遗传结构及区间关系分析,中国农业大学博士学位论文,北京.81-87
马红霞,孙华,郭宁,张海剑,石洁,常佳迎,2018.禾谷镰孢复合种毒素化学型及遗传多样性分析.中国农业科学,51(1):82-95
石洁,王振营,何康来,2005.黄淮海地区夏玉米病虫害发生趋势与原因分析.植物保护,31(5):63-65
石菁,2009.瘤黑粉菌生物学及玉米抗性鉴定研究.甘肃农业大学硕士学位论文,兰州.1-12
孙新素,龙致炜,宋广鹏,陈长青,2017.气候变化对黄淮海地区夏玉米-冬小麦种植模式和产量的影响.中国农业科学,50(13):2476-2487
王建锋,陆宁海,陈长卿,詹刚明,黄丽丽,康振生,2013.陕西省小麦条锈菌群体遗传结构分析.植物病理学报,43(3):294-300
鄢洪海,王琰,张茹琴,夏淑春,王志奎,宋希云,2018.基于ISSR-PCR对山东玉米多堆柄锈菌遗传多样性的研究及其初侵染菌源的推测.菌物学报,37(2):157-165
杨慧,2009.云南糯稻遗传多样性研究.云南农业大学博士学位论文,昆明.24-25
张春民,刘玉英,石洁,张老章,2005.玉米瘤黑粉病抗性鉴定技术研究.玉米科学,3(3):109-111
Evanno G,Regnaut S,Goudet J,2005.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study.Molecular Ecology,14(8):2611-2620
Gan QH,Wang M,Zhang SP,Wang FG,Zhao JR,Zheng YL,Xiao YN,2008.Analysis on genetic and genotypic diversity of populations of Sporisorium reilianum in northern China.Chinese Journal of Biochemistry and Molecular Biology,24(9):846-853(in Chinese)
Guo SB,Huang LL,Kang ZS,Cheng JJ,Lu NH,Yang ZW,Chen YC,2009.Efficacy and mechanism of control of wheat stripe rust by diversifying cultivars in mix-planting.Scientia Agricultura Sinica,10:3485-3492
Guo YY,Chen MG,Sun SL,Wu XF,Jiang K,Zhu ZD,Li HJ,He YQ,Wang XM,2013.Genetic diversity of Puccinia polysora Underw.in China.Scientia Agricultura Sinica,46(21):4523-4533(in Chinese)
Hartl DL,Clark AG,2015.Principles of population genetics.Journal of Animal Breeding&Genetics,117(3):143-144
He XH,Yang J,Wang YY,Zhou HP,Chen JB,Li ZS,Li Y,Zhu YY,2003.Annalysis of genetic structure of Magnaporthe grisea in the fields of different rice varieties.Chinese Journal of Applied Ecology,14(5):733-736(in Chinese)
Jiménez-Becerril MF,Hernández-Delgado S,Solís-Oba M,Juan M,González P,2016.Analysis of mitochondrial genetic diversity of Ustilago maydis in Mexico.Mitochondrial DNA Part A,29(1):1-8
Lai GD,Liu WH,Zhang ZH,Feng X,Lin YL,Liu SC,Qi FB,Lai ZX,2013.Genetic structure of natural populations revealed by ISSR in Myrica rubra from Daiyunshan Mountains.Chinese Journal of Tropical Crops,34(10):1863-1870(in Chinese)
Li HQ,Zhang XW,Jiang XW,Shao XY,Zhao YM,Zhao MA,Song XY,Wang JH,2017.Study on seed quality of maize cultivated varieties in 2016.Seed,36(7):87-91(in Chinese)
Liang JM,2014.Analysis of population genetic structure and regional relationship of Puccinia striiformis f.sp.tritici in Gansu,Sichuan,Ningxia and Shaanxi Provinces of China.PhD Dissertation,China Agricultural University,Beijing.81-87(in Chinese)
Ma HX,Sun H,Guo N,Zhang HJ,Shi J,Chang JY,2018.Analysis of toxigenic chemotype and genetic diversity of the Fusarium graminearum species complex.Scientia Agricultura Sinica,51(1):82-95(in Chinese)
Martínez-Espinoza AD,León-Ramírez CG,Singh N,Ruiz-Herrera J,2003.Use of PCR to detect infection of differentially susceptible maize cultivars using Ustilago maydis strains of variable virulence.International Microbiology:the Official Journal of the Spanish Society for Microbiology,6(2):117-120
Nei M,1993.Analysis of gene diversity in subdivided populations.Proceedings of the National Academy of Sciences,70:3321-3323
Peakall R,Smouse PE,2006.GENALEX 6:genetic analysis in Excel.Population genetic software for teaching and research.Molecular Ecology Notes,6:288-295
Peakall R,Smouse PE,2012.GenAlEx 6.5:genetic analysis in Excel.Population genetic software for teaching and research-an update.Bioinformatics,28:2537-2539
Shi J,2009.Study on Ustilago maydis biology and resistant identification of maize.Master Thesis,Gansu Agriculture University,Lanzhou.1-12(in Chinese)
Shi J,Wang ZY,He KL,2005.Changes and occurrence trend of corn diseases and insect pests in Huang-Huai-Hai summer corn regions.Plant Disease,31(5):63-65(in Chinese)
Sun XS,Long ZW,Song GP,Chen CQ,2017.Effects of climate change on cropping pattern and yield of summer maize-winter wheat in Huang-Huai-Hai Plain.Scientia Agricultura Sinica,50(13):2476-2487(in Chinese)
Wachira FN,Waugh R,Hacker CA,Powell W,1995.Detection of genetic diversity in tea(Camellia sinensis)using RAPDmarkers.Genome,38:201-210
Wang JF,Lu NH,Chen CQ,Zhan GM,Huang LL,Kang ZS,2013.Analysis of population genetic structure of Puccinia striiformis f.sp.tritici in Shaanxi Province,China.Acta Phytopathologica Sinica,43(3):294-300(in Chinese)
Yan HH,Wang Y,Zhang RH,Xia SC,Wang ZK,Song XY,2018.Genetic diversity and deduction of primary infection source of Puccinia polysora in Shandong Province based on ISSR-PCR.Mycosystema,37(2):157-165(in Chinese)
Yang H,2009.Study on genetic diversity of Yunnan glutinous rice.PhD Dissertation,Yunnan Agricultural University,Kunming.24-25(in Chinese)
Yeh FC,Yang RC,Boyle TB,1997.Popgene,the user-friendly shareware for population genetic analysis.University of Alberta,Canada.112-118
Zhang CM,Liu YY,Shi J,Zhang LZ,2005.Inoculation technique for identification of corn resistance to common smut.Journal of Maize Sciences,13(3):109-111(in Chinese)
Zhang MJ,Chen YP,2015.Development of genomic SSRmarkers and analysis of genetic diversity of 40 haploid isolates of Ustilago maydis in China.International Journal of Agriculture and Biology,7:14-30
甘琴华,王明,张绍鹏,王凤格,赵久然,郑用琏,肖炎农,2008.北方玉米丝轴黑粉菌遗传多样性与遗传分化研究.中国生物化学与分子生物学报,24(9):846-853
郭云燕,陈茂功,孙素丽,武小菲,江凯,朱振东,李洪杰,何月秋,王晓明,2013.中国玉米南方锈病病原菌遗传多样性.中国农业科学,46(21):4523-4533
何霞红,杨静,王云月,周惠萍,陈建斌,李作森,李炎,朱有勇,2003.水稻品种多样性田间稻瘟病菌群体遗传结构分析.应用生态学报,14(5):733-736
赖恭梯,刘炜婳,张梓浩,冯新,林玉玲,刘生财,祁芳斌,赖钟雄,2013.戴云山野生杨梅自然群体遗传结构的ISSR分析.热带作物学报,34(10):1863-1870
李贺勤,张晓文,江绪文,邵晓宇,赵延明,赵美爱,宋希云,王建华,2017.2016年中国玉米主产区栽培品种种子质量研究--以黄淮海-华北-西南三区为例.种子,36(7):87-91
梁俊敏,2014.中国甘、川、宁、陕地区小麦条锈菌群体遗传结构及区间关系分析,中国农业大学博士学位论文,北京.81-87
马红霞,孙华,郭宁,张海剑,石洁,常佳迎,2018.禾谷镰孢复合种毒素化学型及遗传多样性分析.中国农业科学,51(1):82-95
石洁,王振营,何康来,2005.黄淮海地区夏玉米病虫害发生趋势与原因分析.植物保护,31(5):63-65
石菁,2009.瘤黑粉菌生物学及玉米抗性鉴定研究.甘肃农业大学硕士学位论文,兰州.1-12
孙新素,龙致炜,宋广鹏,陈长青,2017.气候变化对黄淮海地区夏玉米-冬小麦种植模式和产量的影响.中国农业科学,50(13):2476-2487
王建锋,陆宁海,陈长卿,詹刚明,黄丽丽,康振生,2013.陕西省小麦条锈菌群体遗传结构分析.植物病理学报,43(3):294-300
鄢洪海,王琰,张茹琴,夏淑春,王志奎,宋希云,2018.基于ISSR-PCR对山东玉米多堆柄锈菌遗传多样性的研究及其初侵染菌源的推测.菌物学报,37(2):157-165
杨慧,2009.云南糯稻遗传多样性研究.云南农业大学博士学位论文,昆明.24-25
张春民,刘玉英,石洁,张老章,2005.玉米瘤黑粉病抗性鉴定技术研究.玉米科学,3(3):109-111