HPLC法测定蒙药地格达-4汤中秦皮乙素的含量
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摘要
目的建立HPLC法测定蒙药地格达-4汤中秦皮乙素的含量。方法在单因素实验基础上,采用Box-Behnken响应面法优选出地格达-4汤中秦皮乙素的提取工艺,采用HPLC法测定地格达-4汤中秦皮乙素的含量。色谱条件:πNAP cosmosil C_(18)色谱柱(250 mm×4.6 mm,5μm);以甲醇-1 mL·L~(-1)磷酸(21∶79)为流动相;流速为1.0 mL·min~(-1);检测波长为345 nm;柱温为30℃。结果秦皮乙素质量浓度在3.47～888.00μg·mL~(-1)范围内线性关系良好(r=0.999 8);平均加样回收率为96.0%。结论该方法简便、准确,可有效控制地格达-4汤的质量。
Objective To establish an HPLC method for the determination of aesculetin in Mongolian medicine Digeda-4 Decoction.Methods Based on the results of single factor test,the Box-Behnken design-response surface method was used to optimize the extraction process of aesculetin from Digeda-4 Decoction.The content of aesculetin in Digeda-4 Decoction was determined by HPLC.The chromatographic conditions were as follows:a π NAP cosmosil C_(18)(250 mm×4.6 mm,5 μm) column was used,the mobile phase was a mixture of methanol-1 mL·L~(-1) phosphoric acid(21∶79),the flow rate was 1.0 mL·min~(-1),the detection wavelength was 345 nm,and the column temperature was 30 ℃.Results The method showed good linear relationship within the range of 3.47-888.00 μg·mL~(-1) for aesculetin(r=0.999 8),and the average recovery was 96.0%.Conclusion The method is simple and accurate,and can effectively control the quality of Digeda-4 Decoction.
Objective To establish an HPLC method for the determination of aesculetin in Mongolian medicine Digeda-4 Decoction.Methods Based on the results of single factor test,the Box-Behnken design-response surface method was used to optimize the extraction process of aesculetin from Digeda-4 Decoction.The content of aesculetin in Digeda-4 Decoction was determined by HPLC.The chromatographic conditions were as follows:a π NAP cosmosil C_(18)(250 mm×4.6 mm,5 μm) column was used,the mobile phase was a mixture of methanol-1 mL·L~(-1) phosphoric acid(21∶79),the flow rate was 1.0 mL·min~(-1),the detection wavelength was 345 nm,and the column temperature was 30 ℃.Results The method showed good linear relationship within the range of 3.47-888.00 μg·mL~(-1) for aesculetin(r=0.999 8),and the average recovery was 96.0%.Conclusion The method is simple and accurate,and can effectively control the quality of Digeda-4 Decoction.
引文
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[13] 李红娇,刘婷,孙兴姣,等.响应面法优化蒙药肋柱花总皂苷超声提取工艺[J].中国药业,2018,27(2):26-30.
[14] 徐东伟,王绍辉,刘同祥.Box-Behnken响应面法优化通关藤总皂苷的超声提取工艺及其纯化工艺研究[J].中国药房,2016,27(25):3535-3538.
[15] 马彦江,于兰兰,刘瑞霞,等.响应面法优化天麻多糖的超声提取工艺[J].中国现代中药,2018,20(5):599-603.
[16] 干红女,迟宗良.Box-Behnken响应面法优化四季青的提取工艺[J].中国药师,2017,20(12):2149-2152.
[17] 秦梅颂,周国梁,张丽娟,等.响应面法优化凤尾草中木犀草素的酶法提取工艺[J].中成药,2017,39(12):2622-2624.
[18] 黄庆勇,叶淼,李煌,等.响应面法优选天花粉总皂苷的提取工艺[J].中成药,2015,37(7):1595-1599.
[19] 高意,周光明,张彩虹,等.高效液相色谱法同时测定紫花地丁中6种活性成分[J].食品科学,2016,37(6):101-105.
[20] 陈胡兰,汤沛然,张梅,等.高效液相色谱法测定紫花地丁中秦皮乙素的含量[J].成都中医药大学学报,2007,30(3):57-58,61.
[2] 陈胡兰,董小萍,张梅,等.紫花地丁化学成分研究[J].中草药,2010,41(6):874-877.
[3] 肖苏萍.紫花地丁中的黄酮C-糖苷[J].国外医学中医中药分册,2004,26(6):355.
[4] Bo Qin,QingPing Chen,LuWen Shi,et al.Separation and quantitative determination of three coumarins in the Chinese traditional drug Zihua Diding,Herba Violae,by High Performance Liquid Chromatography[J].J Chin Pharm Sci,1994,3(2):157-163.
[5] 董爱文,朱声文,何征.紫花地丁在不同季节总黄酮含量的变化[J].中国医药工业杂志,2004,35(10):592-593.
[6] 楼之岑,秦波.常用中药材品种整理和质量研究[M].北京:北京医科大学出版社,2003.
[7] 王俊杰,刘弘,张玲昂,等.紫花地丁不同产地及不同部位中秦皮乙素含量比较[J].中国药师,2015,18(8):1400-1402.
[8] 邓晓鸿,董林,李毅.HPLC测定二丁颗粒中秦皮乙素的含量[J].华西药学杂志,2003,18(6):452-453.
[9] 宁火华,艾其茂,罗跃华.HPLC测定紫花地丁中秦皮乙素的含量[J].中国药品标准,2007,8(3):68-69.
[10] 郭晓秋.HPLC法测定紫花地丁中秦皮乙素的含量[J].江西中医药大学学报,2006,18(1):34.
[11] 巴俊杰,荀雅书,李树明.蒙药地格达-4的薄层色谱鉴别[J].时珍国医国药,2006,17(12):2548-2549.
[12] 张玉君,杨晓君,丁泽人,等.内聚能密度结合响应面法优化酿后葡萄皮渣中原花青素的提取工艺[J].西北药学杂志,2018,33(1):19-24.
[13] 李红娇,刘婷,孙兴姣,等.响应面法优化蒙药肋柱花总皂苷超声提取工艺[J].中国药业,2018,27(2):26-30.
[14] 徐东伟,王绍辉,刘同祥.Box-Behnken响应面法优化通关藤总皂苷的超声提取工艺及其纯化工艺研究[J].中国药房,2016,27(25):3535-3538.
[15] 马彦江,于兰兰,刘瑞霞,等.响应面法优化天麻多糖的超声提取工艺[J].中国现代中药,2018,20(5):599-603.
[16] 干红女,迟宗良.Box-Behnken响应面法优化四季青的提取工艺[J].中国药师,2017,20(12):2149-2152.
[17] 秦梅颂,周国梁,张丽娟,等.响应面法优化凤尾草中木犀草素的酶法提取工艺[J].中成药,2017,39(12):2622-2624.
[18] 黄庆勇,叶淼,李煌,等.响应面法优选天花粉总皂苷的提取工艺[J].中成药,2015,37(7):1595-1599.
[19] 高意,周光明,张彩虹,等.高效液相色谱法同时测定紫花地丁中6种活性成分[J].食品科学,2016,37(6):101-105.
[20] 陈胡兰,汤沛然,张梅,等.高效液相色谱法测定紫花地丁中秦皮乙素的含量[J].成都中医药大学学报,2007,30(3):57-58,61.