黑木相思无性系组培生根技术研究
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摘要
为探讨黑木相思组培苗生根的最佳条件,以黑木相思优良无性系组培苗FM25号为材料,采用单因素实验设计方法,分析生根促进剂、MS母液及用量、暗处理时间、温度和有机添加物对其生根情况的影响。结果表明:生根促进剂以IBA和ABT-1种类的处理效果最好;生根效果最佳的母液种类为1/2MS改良(2);暗处理时间以4 d为宜;组培环境的温度设为25℃最适合,生根率可达96.63%;胰蛋白胨、香蕉和椰汁等有机添加物的生根情况均不及对照。
In order to explore the optimal conditions for the rooting of tissue culture seedlings of Acacia melanoxylon, on tissue culture seedlings of superior clones A. melanoxylon, the study was to explore the effects of the rooting promoter, MS mother liquor and its dosage, dark treatment time, temperature and organic additives on its rooting condition in the single-factor experimental design and thus propose a rooting technique system of tissue culture seedlings on A. melanoxylon clones, indicating that IBA and ABT-1 were the rooting promoter with the best treatment; 1/2 MS improvement(2) was registered the best mother liquor for rooting; the dark treatment should be performed for 4 days; 25 ℃ offered the best tissue culture; rooting rate was 96.63%; and the rooting of organic additives such as tryptone, banana and coconut milk was inferior to the control.
In order to explore the optimal conditions for the rooting of tissue culture seedlings of Acacia melanoxylon, on tissue culture seedlings of superior clones A. melanoxylon, the study was to explore the effects of the rooting promoter, MS mother liquor and its dosage, dark treatment time, temperature and organic additives on its rooting condition in the single-factor experimental design and thus propose a rooting technique system of tissue culture seedlings on A. melanoxylon clones, indicating that IBA and ABT-1 were the rooting promoter with the best treatment; 1/2 MS improvement(2) was registered the best mother liquor for rooting; the dark treatment should be performed for 4 days; 25 ℃ offered the best tissue culture; rooting rate was 96.63%; and the rooting of organic additives such as tryptone, banana and coconut milk was inferior to the control.
引文
[1]蒋建.南方主要造林树种养分利用效率研究[D].福州:福建农林大学,2006.
[2]田大伦,沈燕,康文星,等.连栽第1和第2代杉木人工林养分循环的比较[J].生态学报,2011,31(17):5025-5032.
[3]胡峰,施琼,黄烈健.黑木相思愈伤组织诱导及植株再生[J].植物学报,2014,49(5):603-610.
[4]陆茜.杨树连栽对根际土壤环境演变的影响及其自毒效应研究[D].南京:南京林业大学,2016.
[5]黄为炳.建瓯市杉木林栽培存在的问题及对策[J].现代农业科技,2010(15):257-258.
[6]丁国昌,万晓华,杨起帆,等.亚热带树种转换对林地土壤微生物群落结构和功能的影响[J].应用生态学报,2017,28(11):3751-3758.
[7]Hussain M I,González L,Reigosa M J.Allelopathic potential of Acacia melanoxylon on the germination and root growth of native species[J].Weed Biology Management,2011,11:18-28.
[8]裘珍飞.黑木相思不同无性系生根性能比较[J].西部林业科学,2016,45(5):8-11.
[9]Jones C.Getting started in micropropagation of Tasmanian blackwood(Acacia melanoxylon)[J].Combined proceedings-International Plant Propagators’Society,1986,36:477-481.
[10]Meyer H J,Van Staden J.Regeneration of Acacia melanoxylon plantlets in vitro[J].South African journal of botany,1987,53:206-209.
[11]Jones C,Smith D.Effect of 6-benzylaminopurine and1-naphthlacetic acid on in vitro axillary bud development of mature Acacia melanoxylon[J].Combined proceedings-International Plant Propagators’Society,1988,38:389-393.
[12]王盼盼.黑木相思不同优良无性系组培快繁技术比较研究[D].福州:福建农林大学,2011.
[13]戴智明,梁日高,宗亦臣.黑木相思种子无菌体系建立的初探[J].林业实用技术,2012(12):35-36.
[14]施琼.三种相思的组织培养技术研究[D].北京:中国林业科学研究院,2014.
[15]王鸿.三种相思开放式组培快繁及扦插生根技术研究[D].北京:中国林业科学研究院,2017.
[16]裘珍飞,曾炳山,范春节.黑木相思优良无性系(SR17)组培苗茎段再生体系建立[J].分子植物育种,2018,16(15):5023-5028.
[17]刘丽.不同黑木相思优良无性系的组织培养关键技术优化[D].福州:福建农林大学,2013.
[18]范秀平.黑木相思优良无性系组培苗生根技术及其生根机理[D].福州:福建农林大学,2010.
[19]孙英坤,胡绍庆,庞基良,等.珍稀濒危物种堇叶紫金牛高效快繁体系的建立[J].植物学报,2017,52(6):764-773.
[20]张群,吕秀立,何小丽,等.海三棱藨草的组织培养与快繁体系[J].植物学报,2016,51(5):684-690.
[21]马艳,肖娅萍,王彩玲,等.苦皮藤试管苗生根培养研究[J].植物学通报,2004,21(3):332-336.
[2]田大伦,沈燕,康文星,等.连栽第1和第2代杉木人工林养分循环的比较[J].生态学报,2011,31(17):5025-5032.
[3]胡峰,施琼,黄烈健.黑木相思愈伤组织诱导及植株再生[J].植物学报,2014,49(5):603-610.
[4]陆茜.杨树连栽对根际土壤环境演变的影响及其自毒效应研究[D].南京:南京林业大学,2016.
[5]黄为炳.建瓯市杉木林栽培存在的问题及对策[J].现代农业科技,2010(15):257-258.
[6]丁国昌,万晓华,杨起帆,等.亚热带树种转换对林地土壤微生物群落结构和功能的影响[J].应用生态学报,2017,28(11):3751-3758.
[7]Hussain M I,González L,Reigosa M J.Allelopathic potential of Acacia melanoxylon on the germination and root growth of native species[J].Weed Biology Management,2011,11:18-28.
[8]裘珍飞.黑木相思不同无性系生根性能比较[J].西部林业科学,2016,45(5):8-11.
[9]Jones C.Getting started in micropropagation of Tasmanian blackwood(Acacia melanoxylon)[J].Combined proceedings-International Plant Propagators’Society,1986,36:477-481.
[10]Meyer H J,Van Staden J.Regeneration of Acacia melanoxylon plantlets in vitro[J].South African journal of botany,1987,53:206-209.
[11]Jones C,Smith D.Effect of 6-benzylaminopurine and1-naphthlacetic acid on in vitro axillary bud development of mature Acacia melanoxylon[J].Combined proceedings-International Plant Propagators’Society,1988,38:389-393.
[12]王盼盼.黑木相思不同优良无性系组培快繁技术比较研究[D].福州:福建农林大学,2011.
[13]戴智明,梁日高,宗亦臣.黑木相思种子无菌体系建立的初探[J].林业实用技术,2012(12):35-36.
[14]施琼.三种相思的组织培养技术研究[D].北京:中国林业科学研究院,2014.
[15]王鸿.三种相思开放式组培快繁及扦插生根技术研究[D].北京:中国林业科学研究院,2017.
[16]裘珍飞,曾炳山,范春节.黑木相思优良无性系(SR17)组培苗茎段再生体系建立[J].分子植物育种,2018,16(15):5023-5028.
[17]刘丽.不同黑木相思优良无性系的组织培养关键技术优化[D].福州:福建农林大学,2013.
[18]范秀平.黑木相思优良无性系组培苗生根技术及其生根机理[D].福州:福建农林大学,2010.
[19]孙英坤,胡绍庆,庞基良,等.珍稀濒危物种堇叶紫金牛高效快繁体系的建立[J].植物学报,2017,52(6):764-773.
[20]张群,吕秀立,何小丽,等.海三棱藨草的组织培养与快繁体系[J].植物学报,2016,51(5):684-690.
[21]马艳,肖娅萍,王彩玲,等.苦皮藤试管苗生根培养研究[J].植物学通报,2004,21(3):332-336.