参黄降糖汤对成模糖尿病大鼠血糖血脂与内皮脂肪酶及大鼠胸主动脉内皮组织形态影响
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摘要
目的:探讨参黄降糖汤对成模糖尿病(DM)大鼠血糖(BG)血脂与内皮脂肪酶(EL)及大鼠胸主动脉内皮组织形态影响。方法:选取由本院实验动物中心提供的清洁剂Wistar雄性大鼠120只,依据随机数字表法将其分成四组,对照组(30只)、低剂量组(30只)、中剂量组(30只)与高剂量组(30只),使用普通饲料适应性喂养一周后通过注射链脲佐菌素(STZ)和喂食高糖高脂饲料造模;低剂量组、中剂量组和高剂量组大鼠使用参黄降糖汤灌胃,剂量依次是15g/kg、30g/kg、45g/kg,对照组大鼠灌胃使用等容积生理盐水,每天一次,共进行12周;观察大鼠一般状况,血清高密度脂蛋白(HDL)、BG、低密度脂蛋白(LDL)、胆固醇(TC)及EL含量及腹主动脉内皮组织状况。结果:模型组大鼠反应迟钝,精神萎靡,毛色枯黄,体重降低,出现显著多尿、多食及多饮状况;低剂量组大鼠比模型组大鼠各项症状稍好,但不太显著;中、高剂量组大鼠反应稍缓,精神活跃,毛色稍有光泽,尿及饮食降低,体重逐步升高;中、高剂量组大鼠HDL含量较对照组升高、LDL及TC含量较对照组降低,差异均有统计学意义(P<0. 05);中、高剂量大鼠血清EL、BG含量较对照组显著降低,差异有统计学意义(P<0. 05)。结论:参黄降糖汤可改善DM大鼠脂代谢、降低血糖与EL水平,扭转大鼠胸主动脉内皮组织受损情况。
Objective: To analyze the effect of Shenhuang Jiangtang Decoction on blood lipid(BG),lipopolysaccharide(LPS) and the morphology of thoracic aorta for rats with diabetes mellitus(DM). Methods: A total of 120 Wistar male rats were randomly divided into four groups: controlled group(30rats),low dose group(30rats) and middle dose group,30 rats in each group were fed with streptozotocin(STZ). The rats were given Shenhuang Jiangtang Decoction intragastrically in the order of 15g/kg,30g/kg and 45g/kg. The rats in the controlled group were given intragastric administration of normal saline once a day for12 weeks. General status,serum high density lipoprotein(HDL),BG,low density lipoprotein(LDL),cholesterol(TC) and EL content and abdominal aortic endothelium status. Results: The rats in the model group had unresponsiveness,sluggishness,yellowish coat,weight loss and significant polyuria,polyphagia and polyphagia. The symptoms of rats in the low-dose group were slightly better than those in the model group,but not significant. The rats in the high-dose group responded slowly,with active spirit,slightly shiny coat color,reduced urine and diet,and gradually increased body weight. The levels of HDL in middle and high dose groups were higher than those in the controlled group,and the levels of LDL and TC were lower than those in the controlled group(P<0. 05). The contents of EL and BG in the middle and high dose groups were higher than those in the controlled group significantly reduced,the difference was statistically significant(P<0. 05). Conclusion: Shenhuang Jiangtang Decoction can improve lipid metabolism,reduce blood glucose and EL levels,and reverse the damage of thoracic aorta endothelium in rats.
Objective: To analyze the effect of Shenhuang Jiangtang Decoction on blood lipid(BG),lipopolysaccharide(LPS) and the morphology of thoracic aorta for rats with diabetes mellitus(DM). Methods: A total of 120 Wistar male rats were randomly divided into four groups: controlled group(30rats),low dose group(30rats) and middle dose group,30 rats in each group were fed with streptozotocin(STZ). The rats were given Shenhuang Jiangtang Decoction intragastrically in the order of 15g/kg,30g/kg and 45g/kg. The rats in the controlled group were given intragastric administration of normal saline once a day for12 weeks. General status,serum high density lipoprotein(HDL),BG,low density lipoprotein(LDL),cholesterol(TC) and EL content and abdominal aortic endothelium status. Results: The rats in the model group had unresponsiveness,sluggishness,yellowish coat,weight loss and significant polyuria,polyphagia and polyphagia. The symptoms of rats in the low-dose group were slightly better than those in the model group,but not significant. The rats in the high-dose group responded slowly,with active spirit,slightly shiny coat color,reduced urine and diet,and gradually increased body weight. The levels of HDL in middle and high dose groups were higher than those in the controlled group,and the levels of LDL and TC were lower than those in the controlled group(P<0. 05). The contents of EL and BG in the middle and high dose groups were higher than those in the controlled group significantly reduced,the difference was statistically significant(P<0. 05). Conclusion: Shenhuang Jiangtang Decoction can improve lipid metabolism,reduce blood glucose and EL levels,and reverse the damage of thoracic aorta endothelium in rats.
引文
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[11]李彩,林雅军,郭立新.利拉鲁肽对瘦素受体缺陷大鼠主动脉内皮细胞的保护作用及机制[J].中国糖尿病杂志,2017,9(9):567~572
[12] Marques,Silvestre G,Lyra,et al. Bauhinia candicans improves the endothelium-dependent relaxation in aortic rings of alloxan-diabetic Rats/Bauhinia candicans mejora la relajación dependiente de endotelio en anillos de ratas diabético-aloxánicas[J]. Wiener Klinische Wochenschrift,2015,9(5):1~3
[13]胡丽叶,陈红军,宋光耀,等.内皮型一氧化氮合酶在2型糖尿病大鼠主动脉的表达及罗格列酮干预效果[J].解放军医药杂志,2015,3(2):42~46
[14] Li X F,Wan T T,Sun Y M,et al. Na+/Ca2+exchanger inhibitor restores endothelium-dependent relaxation in diabetic rat aorta[J].Exp Clin Endocrinol Diabetes,2015,23(7):394~397
[15]李吉萍,袁野,张文友.西洋参茎叶皂苷对糖尿病大鼠氧化损伤和血管内皮功能的影响[J].中国药理学通报,2017,33(12):173~177
[16]汤日宁,朱冬冬,韩雨晨,等.厄贝沙坦对糖尿病大鼠心肌内皮细胞转分化的作用[J].中华肾脏病杂志,2015,31(5):351~358
[2]仇冬峰,胡云辉,高照,等.多巴胺D 4受体对糖尿病血管内皮损伤的保护作用[J].中华高血压杂志,2016,4(3):226~230
[3] Tang S T,Su H,Zhang Q,et al. Sitagliptin inhibits endothelin-1expression in the aortic endothelium of rats with streptozotocin-induced diabetes by suppressing the nuclear factor-κB/IκBαsystem through the activation of AMP-activated protein kinase[J]. International Journal of Molecular Medicine,2016,37(6):1558~1566
[4]徐静婷,曹娅麟,郑文晖,等.加减一阴煎改善去势大鼠内皮功能紊乱[J].中国病理生理杂志,2015,31(6):1008~1013
[5]郭敏,李世平,张彤哲,等.缬沙坦通过PI3K/Akt/e NOS通路保护2型糖尿病大鼠主动脉内皮细胞免受氧化损伤的研究[J].中国医药导报,2016,13(19):18~21
[6] Chen F,Wu J L,Fu G S,et al. Chronic Treatment With Qiliqiangxin Ameliorates Aortic Endothelial Cell Dysfunction in Diabetic Rats.[J]. Journal of Cardiovascular Pharmacology Therapeutics, 2015, 20(2):230~240
[7]林清飞,王华军,林静,等.虾青素对糖尿病大鼠血管内皮功能的保护作用及机制[J].中华高血压杂志,2015,11(6):530~536
[8] Pramaningtyas M D,Utoro T,Purwono S,et al. Changes on aortic diameter and number of Endothelin-B receptor in aortic endothelium of diabetes mellitus rat model after exercise[J]. Bangladesh Journal of Medical Science,2016,15(3):381~385
[9]赵建刚,黄娜,侯宁宁,等.脂联素对2型糖尿病大鼠主动脉内皮功能影响的研究[J].中国糖尿病杂志,2015,23(3):267~270
[10]任亭,王倩倩,游玉明,等.辣椒素对糖尿病大鼠脂质代谢的影响[J].营养学报,2016,38(6):586~590
[11]李彩,林雅军,郭立新.利拉鲁肽对瘦素受体缺陷大鼠主动脉内皮细胞的保护作用及机制[J].中国糖尿病杂志,2017,9(9):567~572
[12] Marques,Silvestre G,Lyra,et al. Bauhinia candicans improves the endothelium-dependent relaxation in aortic rings of alloxan-diabetic Rats/Bauhinia candicans mejora la relajación dependiente de endotelio en anillos de ratas diabético-aloxánicas[J]. Wiener Klinische Wochenschrift,2015,9(5):1~3
[13]胡丽叶,陈红军,宋光耀,等.内皮型一氧化氮合酶在2型糖尿病大鼠主动脉的表达及罗格列酮干预效果[J].解放军医药杂志,2015,3(2):42~46
[14] Li X F,Wan T T,Sun Y M,et al. Na+/Ca2+exchanger inhibitor restores endothelium-dependent relaxation in diabetic rat aorta[J].Exp Clin Endocrinol Diabetes,2015,23(7):394~397
[15]李吉萍,袁野,张文友.西洋参茎叶皂苷对糖尿病大鼠氧化损伤和血管内皮功能的影响[J].中国药理学通报,2017,33(12):173~177
[16]汤日宁,朱冬冬,韩雨晨,等.厄贝沙坦对糖尿病大鼠心肌内皮细胞转分化的作用[J].中华肾脏病杂志,2015,31(5):351~358