高效液相色谱法测定紫草油中4种植物成分的含量
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摘要
目的:建立高效液相色谱法同时测定紫草油中左旋紫草素、欧前胡素、异欧前胡素和绿原酸含量。方法:以Waters Symmetry C_(18)柱(250 mm×4.6 mm,5μm)为色谱柱,流动相为甲醇(A)-1%甲酸(B)梯度洗脱,流速1.0 ml·min~(-1),检测波长为300 nm和516 nm,柱温30℃,进样量10μl。结果:左旋紫草素、欧前胡素、异欧前胡素、绿原酸分别在10～120μg·ml~(-1)(r=0.998 9)、50～500μg·ml~(-1)(r=0.999 7)、50～500μg·ml~(-1)(r=0.999 6)、5～60μg·ml~(-1)(r=0.999 6)范围呈良好的线性关系。平均加样回收率分别为96.14%(RSD=1.02%)、95.38%(RSD=1.22%)、94.94%(RSD=0.96%)、90.64%(RSD=1.01%)(n=6)。结论:所建立的方法简单、准确、稳定,可用于同时测定紫草油中4种成分的含量。
Objective: To establish an HPLC method for the simultaneous determination of L-shikonin,imperatorin,isoimperatorin and chlorogenic acid in Arnebia root oil. Methods: A Waters Symmetry C_(18) column (250 mm×4.6 mm,5 μm) was used with the mobile phase of methanol (A)-1% formic acid (B) with gradient elution. The flow rate was 1.0 ml·min~(-1),the detection wavelengths were 300 nm and 516 nm performed at room temperature,and the injection volume was 10 μl.Results: L-Shikonin,imperatorin,isoimperatorin and chlorogenic acid showed good linear relationship within the range of 10-120 μg·ml~(-1) (r= 0.998 9),50-500 μg·ml~(-1) (r= 0.999 7),50-500μg·ml~(-1) (r= 0.999 6) and 5-60 μg·ml~(-1) (r = 0.999 6),respectively. The average recovery was 96.14% (RSD =1.02%),95.38% (RSD = 1.22%),94.94% (RSD = 0.96%) and 90.64% (RSD = 1.01%) (n = 6),respectively. Conclusion: The established method is simple,accurate and stable,which can be used for the simultaneous determination of 4 components in Arnebia root oil.
Objective: To establish an HPLC method for the simultaneous determination of L-shikonin,imperatorin,isoimperatorin and chlorogenic acid in Arnebia root oil. Methods: A Waters Symmetry C_(18) column (250 mm×4.6 mm,5 μm) was used with the mobile phase of methanol (A)-1% formic acid (B) with gradient elution. The flow rate was 1.0 ml·min~(-1),the detection wavelengths were 300 nm and 516 nm performed at room temperature,and the injection volume was 10 μl.Results: L-Shikonin,imperatorin,isoimperatorin and chlorogenic acid showed good linear relationship within the range of 10-120 μg·ml~(-1) (r= 0.998 9),50-500 μg·ml~(-1) (r= 0.999 7),50-500μg·ml~(-1) (r= 0.999 6) and 5-60 μg·ml~(-1) (r = 0.999 6),respectively. The average recovery was 96.14% (RSD =1.02%),95.38% (RSD = 1.22%),94.94% (RSD = 0.96%) and 90.64% (RSD = 1.01%) (n = 6),respectively. Conclusion: The established method is simple,accurate and stable,which can be used for the simultaneous determination of 4 components in Arnebia root oil.
引文
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2宓伟,王志强,邱世翠,等.紫草体外抑菌作用研究[J].时珍国医国药,2007,18(9):2217
3夏纯,戴明,游冬阁,等.复方紫草油临床应用探讨[J].世界中医药,2016,11(9):1895-1896
4李光河,杨戒骄.固相萃取-高效液相色谱法同时测定伤疡愈软膏中两种成分含量[J].中国药师,2015,8(18):1387-1389
5赵贺,任君刚,王树巍.紫草油膏的制备工艺筛选[J].哈尔滨商业大学学报,2018,34(5):533-536
6中央军委后勤保障部卫生局.解放军医疗机构制剂规范[M].北京:人民军医出版社,2016:61-63
7姚水宝,黄小黄,杨水新.紫草油膏中左旋紫草素的含量测定[J].中国医院药学杂志,2006,26(7):902-903
8于波涛,宋宗辉,呼永河,等.左旋紫草素体外稳定性研究[J].解放军药学学报,2016,32(1):25-27