甜玉米双酶酶解取汁工艺优化
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摘要
以新鲜甜玉米为原料,中温α-淀粉酶及糖化酶为催化剂,采用双酶法分段酶解制备甜玉米汁。以可溶性固形物含量为评价依据,先考察了液化过程中液料比、中温α-淀粉酶用量、酶解温度和时间对酶解效果的影响,后考察了糖化过程中糖化酶的用量、糖化温度及时间对酶解效果的影响。在单因素试验基础上,通过正交试验优化了甜玉米双酶酶解取汁的最优工艺条件。结果显示最佳条件为:按照料液比为14(g/mL)制取甜玉米浆,中温α-淀粉酶用量为0.35%,于55℃液化25 min;糖化酶用量为0.15%,于55℃下糖化20min;最佳条件下制得的玉米汁可溶性固形物含量为3.90%。该方法具有操作简单、时间短,取汁效果好等优点,可在甜玉米饮料加工中推广和应用。
The sweet corn juice was prepared by using double enzymes method,i.e.catalyzed by medium temperatureα-amylase and saccharifying enzyme.The soluble solid matter content was analyzed as the basis of evaluation.The liquefaction process was investigated in solid- liquid ratio, medium temperature α-amylase dosage,enzymatic hydrolysis temperature and time on the amylolysis effect,and then the effects of the amount of saccharifying enzyme,saccharification temperature and time on the enzyme solution were also studied.On the basis of single factor experiments,the optimum technological conditions of double enzyme hydrolysis for sweet corn were optimized by orthogonal experiment.The results showed that the sweet corn pulp could be produced best,using the solid-liquid ratio of 14(g/mL)and liquefied at 55 ℃for 25 min with 0.35% of medium temperatureα-amylase,then saccharified for 20 min with 0.15% of saccharifying enzyme.The soluble solid matter content of the sweet corn juice prepared under the best condition was 3.90%.This method showed the advantages of simple operation,consuming short time,and effective juice extraction,etc.,and it could be popularized and applied in the processing of sweet corn beverage.
The sweet corn juice was prepared by using double enzymes method,i.e.catalyzed by medium temperatureα-amylase and saccharifying enzyme.The soluble solid matter content was analyzed as the basis of evaluation.The liquefaction process was investigated in solid- liquid ratio, medium temperature α-amylase dosage,enzymatic hydrolysis temperature and time on the amylolysis effect,and then the effects of the amount of saccharifying enzyme,saccharification temperature and time on the enzyme solution were also studied.On the basis of single factor experiments,the optimum technological conditions of double enzyme hydrolysis for sweet corn were optimized by orthogonal experiment.The results showed that the sweet corn pulp could be produced best,using the solid-liquid ratio of 14(g/mL)and liquefied at 55 ℃for 25 min with 0.35% of medium temperatureα-amylase,then saccharified for 20 min with 0.15% of saccharifying enzyme.The soluble solid matter content of the sweet corn juice prepared under the best condition was 3.90%.This method showed the advantages of simple operation,consuming short time,and effective juice extraction,etc.,and it could be popularized and applied in the processing of sweet corn beverage.
引文
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[2]杨婧曦,于海峰.酶法制备白果甜玉米澄清饮料的研究[J].食品科技,2013(9):76-78.
[3]刘晓涛.甜玉米的营养价值及其加工现状的研究[J].技术装备,2009(3):47-48.
[4]刘蔚楠,万忠,甘阳英,等.2015年广东甜玉米产业发展形势与对策建议[J].广东农业科学,2016(3):12-16.
[5]诚夫.世界饮料发展趋势[J].江西食品工业,2002(3):48.
[6]2012年全国饮料总产量为13 024.01万吨[J].饮料工业,2013,16(2):10.
[7]2012年1-12月全国饮料分月总产量及与上年同比[J].饮料工业,2013,16(3):50-52.
[8]谢红涛,余瑞婷,赵瑞娟,等.果蔬汁加工技术进展[J].农产品加工学刊,2010,1(1):76-80.
[9]徐玉娟,肖更生,李升锋,等.甜玉米汁酶解工艺条件的研究[J].广东农业科学,2006(11):48-50.
[10]李次力,缪铭.酶解甜玉米糖化液开发营养性饮料的研究[J].食品科学,2005,26(5):175-178.
[11]邓开野,麦伟浩.甜玉米饮料的加工工艺研究[J].广西轻工业,2009(2):1-2.
[12]陶兴无.α-淀粉酶在甜玉米饮料生产中的应用研究[J].湖北工业大学学报,2006,21(2):17-20.
[13]罗映英,宋小峰,王凤仙,等.甜玉米饮料的工艺配方优化[J].农产品工程,2015(7):55-57.