GA_3与ABA对黑穗醋栗二次萌芽总酚含量及代谢酶活性的影响
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摘要
【目的】了解黑穗醋栗发生二次萌芽过程中总酚含量及其合成代谢关键酶活性的变化规律,为揭示黑穗醋栗发生二次萌芽的内在机理及其人工调控提供科学依据。【方法】以易发生二次萌芽的黑穗醋栗品种亚德和不易发生二次萌芽的黑穗醋栗品种布劳德为试材,使用30.0 mg/L赤霉素(GA3)和50.0 mg/L脱落酸(ABA)进行叶面喷施处理,以喷清水为对照(CK1和CK2),利用Folin-酚比色法和酶联免疫分析试剂盒测定各处理黑穗醋栗品种二次萌芽的总酚含量及关键酶活性。【结果】在黑穗醋栗品种亚德和布劳德发生二次萌芽过程中,总酚含量及苯丙氨酸解氨酶(PAL)、查尔酮异构酶(CHI)和肉桂酸-4-羟化酶(C4H)活性降低,多酚氧化酶(PPO)活性升高;经30.0 mg/L GA3处理后亚德和布劳德二次萌芽的总酚含量明显低于CK1和CK2,PAL和CHI活性明显低于CK1和CK2,PPO活性明显高于CK1和CK2,C4H活性总体上低于CK1和CK2;经50.0 mg/L ABA处理后亚德二次萌芽的总酚含量显著高于GA3处理和CK1(P<0.05,下同),经50.0 mg/L ABA处理亚德各测定日期(除8月17日外)的总酚含量均显著高于GA3处理,且明显高于CK2;50.0 mg/L ABA处理的亚德和布劳德PAL活性明显高于GA3处理及CK1和CK2,PPO活性除8月17日外均显著低于GA3处理,且明显低于CK1和CK2,CHI和C4H活性总体上明显高于GA3处理及CK1和CK2。【结论】叶面喷施50.0 mg/L ABA可提高黑穗醋栗品种亚德和布劳德二次萌芽的代谢关键酶PAL、CHI和C4H活性,降低PPO活性,促进总酚合成并抑制其氧化分解,从而抑制黑穗醋栗品种亚德和布劳德发生二次萌芽。
【Objective】The aim of the study was to investigate the changes of total phenolic content and anabolic key enzyme activities during the secondary bud burst of black currant,and provide a scientific basis for revealing the internal mechanism and artificial regulation of secondary bud burst.【Method】The black currant variety Adelinia which was easily to be germinated twice and the black currant variety Brodtrop which was not easily to be germinated twice were used as test materials,and foliar spray treatment with 30.0 mg/L gibberellin(GA3)and 50.0 mg/L abscisic acid(ABA)was set,and water was used as control(CK). Folin-phenol colorimetric assay and enzyme-linked immunosorbent assay kit were used to determine the total phenolic content and its key enzyme activity in secondary bud burst.【Result】During the secondary bud burst of the black currant varieties Adelinia and Brodtrop,the total phenolic content decreased gradually,phenylalanine ammonia lyase(PAL),chalcone isomerase(CHI) and cinnamic acid-4-hydroxyl(C4 H)activities were decreased,and the activity of polyphenol oxidase(PPO)was increased. The decrease speed of total phenolic content of 30.0 mg/L GA3 treated Adelinia and Brodtrop was faster than that of CK1 and CK2,respectively. The activities of PAL and CHI were lower than that of CK1 and CK2. PPO activity was higher than CK1 and CK2,C4 H activity was lower than CK1 and CK2. The total phenolic content of 50.0 mg/L ABA treated Adelinia secondary bud burst was significantly higher than GA3 treatment and CK1(P<0.05,the same below),and total phenolic content on the date of determination(except Brodtrop on August 17)was significantly higher than that of GA3 treatment,and was also higher than CK2. The PAL activities of 50.0 mg/L ABA treated Adelinia and Brodtrop were higher than GA3 treatment,CK1 and CK2,PPO activity except on August 17 was significantly lower than GA3 treatment,was greatly lower than CK1 and CK2,CHI and C4 H activity was higher than GA3 treatment,CK1 and CK2.【Conclusion】Foliar application of 50.0 mg/L ABA inhibits the activity of key enzymes PAL,CHI and C4 H in the secondary bud burst of Adelinia and Brodtrop,promotes PPO activity of key enzymes,promotes total phenol synthesis and inhibits total phenol oxidation,thereby inhibiting the secondary bud burst of Adelinia and Brodtrop.
【Objective】The aim of the study was to investigate the changes of total phenolic content and anabolic key enzyme activities during the secondary bud burst of black currant,and provide a scientific basis for revealing the internal mechanism and artificial regulation of secondary bud burst.【Method】The black currant variety Adelinia which was easily to be germinated twice and the black currant variety Brodtrop which was not easily to be germinated twice were used as test materials,and foliar spray treatment with 30.0 mg/L gibberellin(GA3)and 50.0 mg/L abscisic acid(ABA)was set,and water was used as control(CK). Folin-phenol colorimetric assay and enzyme-linked immunosorbent assay kit were used to determine the total phenolic content and its key enzyme activity in secondary bud burst.【Result】During the secondary bud burst of the black currant varieties Adelinia and Brodtrop,the total phenolic content decreased gradually,phenylalanine ammonia lyase(PAL),chalcone isomerase(CHI) and cinnamic acid-4-hydroxyl(C4 H)activities were decreased,and the activity of polyphenol oxidase(PPO)was increased. The decrease speed of total phenolic content of 30.0 mg/L GA3 treated Adelinia and Brodtrop was faster than that of CK1 and CK2,respectively. The activities of PAL and CHI were lower than that of CK1 and CK2. PPO activity was higher than CK1 and CK2,C4 H activity was lower than CK1 and CK2. The total phenolic content of 50.0 mg/L ABA treated Adelinia secondary bud burst was significantly higher than GA3 treatment and CK1(P<0.05,the same below),and total phenolic content on the date of determination(except Brodtrop on August 17)was significantly higher than that of GA3 treatment,and was also higher than CK2. The PAL activities of 50.0 mg/L ABA treated Adelinia and Brodtrop were higher than GA3 treatment,CK1 and CK2,PPO activity except on August 17 was significantly lower than GA3 treatment,was greatly lower than CK1 and CK2,CHI and C4 H activity was higher than GA3 treatment,CK1 and CK2.【Conclusion】Foliar application of 50.0 mg/L ABA inhibits the activity of key enzymes PAL,CHI and C4 H in the secondary bud burst of Adelinia and Brodtrop,promotes PPO activity of key enzymes,promotes total phenol synthesis and inhibits total phenol oxidation,thereby inhibiting the secondary bud burst of Adelinia and Brodtrop.
引文
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胡习祯,徐晓燕.2013.黑加仑的营养价值及产品开发[J].轻工科技,(1):8-9.[Hu X Z,Xu X Y.2013.Nutritional value and product development of blackcurrant[J].Light Industry Science and Technology,(1):8-9.]
霍俊伟,李著花,秦栋.2011.黑穗醋栗营养成分和保健功能及产业发展前景[J].东北农业大学学报,42(2):139-144.[Huo J W,Li Z H,Qin D.2011.Review of nutritional ingredients and health protectal function of black currant fruit and its prospect in industrial development[J].Journal of Northeast Agricultural University,42(2):139-144.]
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李芳晓,李曙雷,秦栋,战歌,霍俊伟,王欢欢,赵毅,韩伟,步志鹏.2014.黑穗醋栗ASA含量及其代谢酶活性差异分析[J].南方农业学报,45(7):1237-1241.[Li F X,Li SL,Qin D,Zhan G,Huo J W,Wang H H,Zhao Y,Han W,Bu Z P.2014.Ascorbic acid content and difference of related enzyme activities in fruit of blackcurrant[J].Journal of Southern Agriculture,45(7):1237-1241.]
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李雪,潘学军,张文娥.2016.UV辐射对植物多酚代谢的影响[J].山地农业生物学报,35(6):54-60.[Li X,Pan X J,Zhang W E.2016.Effect of UV radiation on the metabolism and the pathway of plant polyphenol[J].Journal of Mountain Agriculture and Biology,35(6):54-60.]
贾丽丽,路金才.2008.黑加仑的药用研究进展[J].中国中医药信息杂志,15(S):110-112.[Jia L L,Lu J C.2008.Medicinal research on black currant[J].Chinese Journal of Information on Traditional Chinese Medicine,15(S):110-112.]
马君兰,李成,魏颖,李莉,赵越.2007.异黄酮的生物合成途径及其调控[J].东北农业大学学报,38(5):692-696.[Ma J L,Li C,Wei Y,Li L,Zhao Y.2007.The pathway of isoflavone biosynthesis and its regulation[J].Journal of Northeast Agricultural University,38(5):692-696.]
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秦栋,张椿浩,刘庆帅,薛晓晓,霍俊伟,员盎然.2017.ABA和GA3对黑穗醋栗二次萌芽超微结构的影响[J].果树学报,34(10):1301-1308.[Qin D,Zhang C H,Liu Q S,Xue X X,Huo J W,Yuan A R.2017.Effect of GA3and ABA on cell ultra-structure of the secondary-bursting buds in black currant(Ribes nigrum)[J].Journal of Fruit Science,34(10):1301-1308.]
王海波.2006.桃芽自然休眠诱导与短时间高温破眠机制研究[D].乌鲁木齐:新疆农业大学.[Wang H B.2006.Study on the mechanisms of endodormancy induction and shortterm heating releasing endodormancy in peach(Prunus persica)bud[D].Urumqi:Xinjiang Agricultural University.]
王欢欢,霍俊伟,秦栋,谢福春,张永和,李兴国.2015.赤霉素与脱落酸对黑穗醋栗二次萌芽形态指标的影响[J].北方园艺,(18):13-17.[Wang H H,Huo J W,Qin D,Xie F C,Zhang Y H,Li X G.2015.Effect of GA3and ABAon morphological indexes of secondary germination in Ribes nigum L.[J].Northern Horticulture,(18):13-17.]
王瑞,于洪侠,祁永会.2016.黑穗醋栗开发利用现状及发展前景[J].现代农业研究,(1):30.[Wang R,Yu H X,Qi Y H.2016.Development and utilization of black currant(Ribes nigum L.)and its development prospects[J].Modern Agricultural Research,(1):30.]
魏海蓉,高东升,李宪利.2005a.甜樱桃芽酚类物质含量及相关酶活性变化与自然休眠的关系[J].园艺学报,32(2):197-201.[Wei H R,Gao D S,Li X L.2005a.The changes of phenolics and related enzymes activity in sweet cherry buds during endo-dormancy period[J].Acta Horticulturae Sinica,32(2):197-201.]
魏海蓉,高东升,李宪利.2005b.植物调节剂对甜樱桃休眠的调控及花芽酚类物质含量的影响[J].园艺学报,32(4):584-588.[Wei H R,Gao D S,Li X L.2005b.Effects of plant growth regulators on the content of phenolics in sweet cherry dormant flower buds and dormancy[J].Acta Horticulturae Sinica,32(4):584-588.]
魏海蓉,高东升,李宪利.2006.剥鳞和化学药剂处理对甜樱桃花芽休眠及酚类物质的影响[J].园艺学报,33(4):817-820.[Wei H R,Gao D S,Li X L.2006.The effects of bud-scale removing and chemical treatments on dormancy-release and phenolics of sweet cherry flower buds[J].Acta Horticulturae Sinica,33(4):817-820.]
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