不同产地葛根与粉葛的HPLC指纹图谱及其多成分化学模式识别分析
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摘要
目的:建立葛根与粉葛的HPLC特征图谱,结合化学模式识别方法对不同产区的葛根与粉葛进行区分,并鉴别葛根产区,为科学评价和有效控制二者的质量提供参考。方法:利用HPLC检测23批葛根或粉葛药材,采用"中药色谱指纹图谱相似度评价系统"(2004A版)软件进行相似度评价,建立共有模式。以其色谱峰信息为数据来源,利用3种化学模式识别方法综合分析葛根和粉葛药材的质量。结果:除了样品S22(陕西产地)之外,不同产区的葛根和粉葛的相似度均> 0.9;说明葛根与粉葛药材的相似度良好,采用特征图谱相似度评价方法无法区分葛根与粉葛药材。利用主成分分析只能鉴别葛根与粉葛;偏最小二乘法-判别分析其模型解释度96.4%,预测度74.6%,能够区分葛根与粉葛,并能区分葛根的产区;系统聚类分析与偏最小二乘法-判别分析结果较为一致。结论:利用化学模式识别方法可快速区分葛根与粉葛,鉴别葛根产区,适用于葛根与粉葛的药材质量评价。
Objective:To establish HPLC specific chromatograms of Puerariae Lobatae Radix(PLR)and Puerariae Thomsonii Radix(PTR),and make a distinction about their species and different habitats of PLR by chemical pattern recognition,provide reliable methods for scientific evaluation and effective control of their quality.Method:HPLC was employed to determine the contents of chemical ingredients in 23 batches of PLR and PTR.The similarity analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica"(version of 2004 A),then a common pattern was established.Based on its chemical fingerprint information,the quality of PLR and PTR was comprehensively analyzed by three kinds of chemical pattern recognition methods.Result:In addition to sample S22(from Shaanxi province),the similarities of 23 batches of samples were more than 0.9,which showed that similarity of PLR and PTR was good,this method can not differentiate them.Principal component analysis(PCA) could only identify PLR and PTR,but partial least squares-discriminant analysis(PLS-DA) could distinguish PLR from PTR and the producing areas of PLR with model interpretation of 96.4% and prediction of 74.6%.The result of hierarchical cluster analysis(HCA) was consistent with PLS-DA.Conclusion:Chemical pattern recognition method can make a distinction between PLR and PTR,as well as different habitats of PLR;it is suitable for quality control of their medicinal materials.
Objective:To establish HPLC specific chromatograms of Puerariae Lobatae Radix(PLR)and Puerariae Thomsonii Radix(PTR),and make a distinction about their species and different habitats of PLR by chemical pattern recognition,provide reliable methods for scientific evaluation and effective control of their quality.Method:HPLC was employed to determine the contents of chemical ingredients in 23 batches of PLR and PTR.The similarity analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica"(version of 2004 A),then a common pattern was established.Based on its chemical fingerprint information,the quality of PLR and PTR was comprehensively analyzed by three kinds of chemical pattern recognition methods.Result:In addition to sample S22(from Shaanxi province),the similarities of 23 batches of samples were more than 0.9,which showed that similarity of PLR and PTR was good,this method can not differentiate them.Principal component analysis(PCA) could only identify PLR and PTR,but partial least squares-discriminant analysis(PLS-DA) could distinguish PLR from PTR and the producing areas of PLR with model interpretation of 96.4% and prediction of 74.6%.The result of hierarchical cluster analysis(HCA) was consistent with PLS-DA.Conclusion:Chemical pattern recognition method can make a distinction between PLR and PTR,as well as different habitats of PLR;it is suitable for quality control of their medicinal materials.
引文
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[2]邹东洋,董桂英.葛根治疗心脑血管病的研究进展[J].中西医结合心脑血管病杂志,2017,15(23):2992-2993.
[3]魏述永.葛根素心血管保护作用及其机制研究进展[J].中国中药杂志,2015,40(12):2278-2284.
[4]谭家林,文颖娟,樊一波.葛根及其复方治疗糖尿病心肌病研究进展[J].亚太传统医药,2018,14(1):76-78.
[5]李昕,潘俊娴,陈士国,等.两个品种葛根化学成分及体外抗氧化性的分析[J].中国食品学报,2017,17(2):274-280.
[6]于海帅.基于主成分分析、聚类分析和典型相关分析的漏芦抗胃癌谱效关系探索[J].中国实验方剂学杂志,2016,22(21):27-31.
[7]肖榕,林艳,雷思敏,等.UPLC指纹图谱结合模式识别分析不同产地生/制何首乌[J].中国中药杂志,2017,42(12):2305-2310.
[8]吴一凡,肖新立.不同产地甘草中主要微量元素及系统聚类分析[J].临床合理用药杂志,2017,10(27):96-97.
[9]陈安珍,蒋万枫,袁航,等.基于超高效液相色谱偏最小二乘判别分析法建立鉴别大黄真伪及种属预测模型的方法[J].中国药学杂志,2016,41(3):197-202.
[10]陈舒妤,石婧婧,邹立思,等.UFLC-Q-TRAP-MS/MS同时测定五味子中木脂素及有机酸类成分[J].中国中药杂志,2018,43(10):2104-2111.
[11]孙长海,杨伟丽,郑苹,等.细叶杜香挥发油GC-MS与直观推导式演进特征投影法分析[J].中草药,2009,40(10):1556-1558.
[12]韩胜男,张晓杭,周培培,等.化学计量学在中药组效关系研究中的应用进展[J].中国中药杂志,2014,39(14):2595-2602.
[13]徐彤,黄萌萌,陈家进,等.安宫牛黄丸HPLC指纹图谱及化学模式识别分析[J].中国实验方剂学杂志,2017,23(20):14-19.
[14]张晓冬,陈龙,白玉,等.近红外光谱结合主成分分析和聚类分析鉴别炉甘石生品、伪品和炮制品[J].中国实验方剂学杂志,2018,24(12):1-8.
[15]苏素娇,李娜,陈亮,等.藤茶的HPLC指纹图谱及化学模式识别研究[J].中国实验方剂学杂志,2013,19(22):144-147.
[16]李时珍.本草纲目[M].北京:人民卫生出版社,2004:780-945.